144 research outputs found
Synthesis of Oligodeoxyriboâ and Oligoribonucleotides According to the HâPhosphonate Method
Oligonucleotides can be synthesized by condensing a protected nucleoside Hâphosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside Hâphosphonate diester. This can then be converted to a dinucleoside phosphate or to a backboneâmodified analog such as a phosphorothioate or phosphoramidite. This unit discusses four alternative methods for synthesizing nucleoside Hâphosphonate monoesters. The methods are efficient and experimentally simple, and use readily available reagents. The unit describes the activation of the monoesters, as well as competing acylation and other potential side reactions.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143594/1/cpnc0304.pd
Synthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyriboâ and Oligoribonucleotides According to the H
Oligonucleotides can be synthesized by condensing a protected nucleoside Hâphosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside Hâphosphonate diester. This can then be converted to a dinucleoside phosphate or to a backboneâmodified analog such as a phosphorothioate or phosphoramidite. This unit discusses four alternative methods for synthesizing nucleoside Hâphosphonate monoesters. The methods are efficient and experimentally simple, and use readily available reagents. The unit describes the activation of the monoesters, as well as competing acylation and other potential side reactions.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143625/1/cpnc0304.pd
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