Electromyographical Analysis of Lower Extremity Muscle Activation During Variations of the Loaded Step-Up Exercise

The loaded step-up exercise allows strength and conditioning practitioners to incorporate a unilateral resistance for athletes while performing extension at the hip, knee, and plantar flexion at the ankle. This study evaluated the activation of the biceps femoris (BF), gluteus maximus (GMx), gluteus medius (GMe), rectus femoris, semitendinosus (ST), vastus lateralis, and vastus medialis during 4 variations of the step-up exercise to assess the specific muscle training stimulus of each exercise variation. The exercises included the step-up, crossover step-up, diagonal step-up, and lateral step-up. Fifteen women who regularly engaged in lower body resistance training performed the 4 exercises with 6 repetition maximum loads on a 45.72-cm (18-in.) plyometric box. Data were collected with a telemetered electromyography (EMG) system, and root mean square values were calculated for EMG data for eccentric and concentric phases. Results of a repeated-measures analysis of variance revealed a variety of differences in muscle activation between the exercises (

FOURTH NORTH AMERICAN SURVEY OF UNDERGRADUATE BIOMECHANICS INSTRUCTION IN KINESIOLOGY/EXERCISE SCIENCE

A web-based survey of introductory biomechanics instructors in Kinesiology/Exercise Science departments from North America was conducted to document current instructor and course characteristics. Responses from 165 faculty from the USA and Canada were compiled and compared to previous surveys. Survey respondents tended to have doctoral training in biomechanics and reported nominally larger percentages, relative to previous surveys, of courses entitled “biomechanics” and mechanical content. The respondents also reported greater diversity of course prerequisites, less emphasis on anatomy, and less familiarity with NASPE guidelines for the course than reported in previous surveys

THE EFFECT OF PLYOMETRIC TRAINING VOLUME ON JUMPING PERFORMANCE

This study compared high and low daily volume periodized plyometric training programs and their effect on countermovement jump (CMJ) performance over a 2 week testing time course after training. Thirty-five male subjects participated. Subjects CMJ was tested on a force platform prior to and at 2, 6, 10 and 14 days post training. Peak vertical ground reaction force (GRF), reactive strength index-modified (RSI mod), jump height (JH), and body mass were assessed. No differences were found between the high and low volume training groups. Subjects GRF, RSI mod, and JH performance was improved in a range of 6 to 14 days post- training. Low and high volume periodized plyometric training programs produced equal results. Six to 14 days of recovery post training was required to accrue the training benefit

Diurnal Variation in Stretch Shortening Cycle and Non-Stretch Shortening Cycle Jumping Performance

This study assessed the diurnal variations, as well as the subjects’ perceptions of how time of day affects their jumping performance. Eighteen subjects participated in testing sessions at 7 a.m., noon, and 5 p.m., on the same day. Subjects performed squat jumps (SJ) and countermovement jumps (CMJ) on a force platform with peak ground reaction force (GRF), jump height, and reactive strength index-modified (RSImod) obtained for each jump. Diurnal differences for the CMJ were found for GRF (P = 0.048). Diurnal differences for the SJ were found in RSImod (P= 0.04). Subjective assessment of jumping explosiveness was significantly different across time of day (P = 0. 047). Lower body power training such as plyometrics, may be more effective at mid day than early morning or late afternoon

ANTAGONIST CONDITIONING CONTRACTIONS IMPAIR AGONIST FUNCTIONING

This study assessed the effect of antagonist conditioning contractions (ACC) on the subsequent force and electromyography of an agonist. Twelve subjects performed isokinetic elbow flexion on a dynamometer in 4 test conditions including a baseline condition without, and 1, 3 and 6 seconds after, isometric triceps extension. Average peak torque (T), peak torque/body weight (T:BW), average power (P), and rate of torque development (RTD) were assessed. Electromyographic data were obtained from elbow extensors and flexors. A repeated measures ANOVA with post hoc analysis demonstrated that T, T:BW, P, and RTD were higher in the baseline, compared to the post ACC conditions (P ≤ 0.05), and appears to be due to higher brachioradialis activation in the baseline condition in compared to some post ACC conditions (P ≤ 0.05)

The APOEε3/ε4 Genotype Drives Distinct Gene Signatures in the Cortex of Young Mice

Introduction: Restrictions on existing APOE mouse models have impacted research toward understanding the strongest genetic risk factor contributing to Alzheimer\u27s disease (AD) and dementia, APOEε4 , by hindering observation of a key, common genotype in humans - APOEε3/ε4 . Human studies are typically underpowered to address APOEε4 allele risk as the APOEε4/ε4 genotype is rare, which leaves human and mouse research unsupported to evaluate the APOEε3/ε4 genotype on molecular and pathological risk for AD and dementia. Methods: As a part of MODEL-AD, we created and validated new versions of humanized APOEε3/ε3 and APOEε4/ε4 mouse strains that, due to unrestricted breeding, allow for the evaluation of the APOEε3/ε4 genotype. As biometric measures are often translatable between mouse and human, we profiled circulating lipid concentrations. We also performed transcriptional profiling of the cerebral cortex at 2 and 4 months (mos), comparing APOEε3/ε4 and APOEε4/ε4 to the reference APOEε3/ε3 using linear modeling and WGCNA. Further, APOE mice were exercised and compared to litter-matched sedentary controls, to evaluate the interaction between APOEε4 and exercise at a young age. Results: Expression of human APOE isoforms were confirmed in APOEε3/ε3, APOEε3/ε4 and APOEε4/ε4 mouse brains. At two mos, cholesterol composition was influenced by sex, but not APOE genotype. Results show that the APOEε3/ε4 and APOEε4/ε4 genotype exert differential effects on cortical gene expression. APOEε3/ε4 uniquely impacts \u27hormone regulation\u27 and \u27insulin signaling,\u27 terms absent in APOEε4/ε4 data. At four mos, cholesterol and triglyceride levels were affected by sex and activity, with only triglyceride levels influenced by APOE genotype. Linear modeling revealed APOEε3/ε4 , but not APOEε4/ε4 , affected \u27extracellular matrix\u27 and \u27blood coagulation\u27 related terms. We confirmed these results using WGCNA, indicating robust, yet subtle, transcriptional patterns. While there was little evidence of APOE genotype by exercise interaction on the cortical transcriptome at this young age, running was predicted to affect myelination and gliogenesis, independent of APOE genotype with few APOE genotype-specific affects identified. Discussion: APOEε4 allele dosage-specific effects were observed in circulating lipid levels and cortical transcriptional profiles. Future studies are needed to establish how these data may contribute to therapeutic development in APOEε3/ε4 and APOEε4/ε4 dementia patients

Usefulness and limitation of dobutamine stress echocardiography to predict acute response to cardiac resynchronization therapy.

peer reviewedBackground: It has been hypothesized that a long-term response to cardiac resynchronization therapy (CRT) could correlate with myocardial viability in patients with left ventricular (LV) dysfunction. Contractile reserve and viability in the region of the pacing lead have not been investigated in regard to acute response after CRT. Methods: Fifty-one consecutive patients with advanced heart failure, LV ejection fraction ≤ 35%, QRS duration > 120 ms, and intraventricular asynchronism ≥ 50 ms were prospectively included. The week before CRT implantation, the presence of viability was evaluated using dobutamine stress echocardiography. Acute responders were defined as a ≥15% increase in LV stroke volume. Results: The average of viable segments was 5.8 ± 1.9 in responders and 3.9 ± 3 in nonresponders (P = 0.03). Viability in the region of the pacing lead had an excellent sensitivity (96%), but a low specificity (56%) to predict acute response to CRT. Mitral regurgitation (MR) was reduced in 21 patients (84%) with acute response. The presence of MR was a poor predictor of response (sensibility 93% and specificity 17%). However, combining the presence of MR and viability in the region of the pacing lead yields a sensibility (89%) and a specificity (70%) to predict acute response to CRT. Conclusion: Myocardial viability is an important factor influencing acute hemodynamic response to CRT. In acute responders, significant MR reduction is frequent. The combined presence of MR and viability in the region of the pacing lead predicts acute response to CRT with the best accuracy

A novel systems biology approach to evaluate mouse models of late-onset Alzheimer\u27s disease.

BACKGROUND: Late-onset Alzheimer\u27s disease (LOAD) is the most common form of dementia worldwide. To date, animal models of Alzheimer\u27s have focused on rare familial mutations, due to a lack of frank neuropathology from models based on common disease genes. Recent multi-cohort studies of postmortem human brain transcriptomes have identified a set of 30 gene co-expression modules associated with LOAD, providing a molecular catalog of relevant endophenotypes. RESULTS: This resource enables precise gene-based alignment between new animal models and human molecular signatures of disease. Here, we describe a new resource to efficiently screen mouse models for LOAD relevance. A new NanoString nCounter® Mouse AD panel was designed to correlate key human disease processes and pathways with mRNA from mouse brains. Analysis of the 5xFAD mouse, a widely used amyloid pathology model, and three mouse models based on LOAD genetics carrying APOE4 and TREM2*R47H alleles demonstrated overlaps with distinct human AD modules that, in turn, were functionally enriched in key disease-associated pathways. Comprehensive comparison with full transcriptome data from same-sample RNA-Seq showed strong correlation between gene expression changes independent of experimental platform. CONCLUSIONS: Taken together, we show that the nCounter Mouse AD panel offers a rapid, cost-effective and highly reproducible approach to assess disease relevance of potential LOAD mouse models