Fidelización de usuarios mayores en centros de fitness: gestión de clientes por programas de actividad física

Los centros de fitness, son hoy en día lugares donde los socios realizan actividad física «controlada», donde mejoran su estado físico y mental, incluso en donde se socializan con otros socios de todas las edades. Estos centros, han sido normalmente gestionados por personas sin formación en la materia y con el único interés de tener socios, sin tener en cuenta la repercusión a largo plazo. Este interés, sumado a problemas de gestión del centro, y a problemas personales de los socios, ha propiciado que existan altas tasas de deserción en este tipo de centros indoor, con la consecuente elevación de los gastos en marketing, no obteniendo así, una rentabilidad sostenible. Proponemos, un programa de actividad física con el que se podría mejorar estas tasas de fidelización, siendo el punto central de la gestión de los centros de hoy en día, la satisfacción y la lealtad a los centros de fitnes

Heparan sulfate proteoglycans undergo differential expression alterations in left sided colorectal cancer, depending on their metastatic character

Abstract Background Heparan sulfate proteoglycans (HSPGs) are complex molecules which play a role in the invasion and growth and metastatic properties of cancerous cells. In this work we analyze changes in the patterns of expression of HSPGs in left sided colorectal cancer (LSCRC), both metastatic and non-metastatic, and the results are also compared with those previously obtained for right sided tumors (RSCRCs). Methods Eighteen LSCRCs were studied using qPCR to analyze the expression of both the proteoglycan core proteins and the enzymes involved in heparan sulfate chain biosynthesis. Certain HSPGs also carry chondroitin sulfate chains and so we also studied the genes involved in its biosynthesis. The expression of certain genes that showed significant expression differences were also analysed using immunohistochemical techniques. Results Changes in proteoglycan core proteins were dependent on their location, and the main differences between metastatic and non-metastatic tumors affected cell-surface glypicans, while other molecules were quite similar. Glypicans were also responsible for the main differences between RS- and LS- malignances. Regarding the biosynthesis of heparan sulfate chains, differential alterations in transcription depending on the presence or not of metastasis affected genes involved in the modification of uronic acid (epimerization and 2-O sulfation), and some isoforms responsible for sulfation of glucosamine (NDST1, HS6ST1). Moreover, in RSCRCs differences were preferentially found in the expression of genes involved in C6 and C3 sulfation of glucosamine, but not in NDSTs or SULFs. Finally, synthesis of chondroitin sulfate showed some alterations, which affected various steps, including polimerization and the modification of chains, but the main variations dependent on the presence of metastases were epimerization and 6C sulfation; however, when compared with RSCRCs, the essential divergences affected polymerization of the chains and the 6C sulfation of the galactosamine residue. Conclusions We evidenced alterations in the expression of HSPGs, including the expression of cell surface core proteins, many glycosiltransferases and some enzymes that modify the GAG chains in LSCRCs, but this was dependent on the metastatic nature of the tumor. Some of these alterations are shared with RSCRCs, while others, focused on specific gene groups, are dependent on tumor localization

New system for measuring impact vibration on floor decking sheets

The present paper exposes an alternative system more simple and economic, consisting of a predefined beating device and a sensor able to determine, once produced the hit, the energy absorbed by the plate. After the impact being produced, the plate undergoes a deformation which absorbs part of the energy, being the reminding one transmitted to the slab and, at the same time, causing induced airborne noise in the adjoining room

Professional preferences of students in physical education and sport sciences

The actual context has enhanced job opportunities in the field of sport in order to respond to the current market demand. Thus, Physical Education and Sport Science graduates who begin to do differents jobs to the traditional ones but relate to their study field. The aim of this study was to guess which are the job preferences of the students of Physical Education and Sport Science of Seville University by gender and age doing the second cycle of their college degree and determine if there are significant differences. A descriptive analysis was carried out, using a questionnaire based on several researches, it was related to professional opportunities in sport sciences. The sample was of 118 students which represented 40.7% of the overall registered students. Results shown that sport management is the most preferable professional opportunity for women and men of the total sample, following in second place by teaching in secondary school for people older than 25 years of both sexes and teaching in primary school for the younger than 25 years. These findings announce changes in occupational trends in sports, to be taken into account in the framework of the European higher education (Degree of Science in Sport and Physical Activity, own US Masters and Official, lifelong learning programs...)

Género y sexo como variables moduladoras del comportamiento sexual en jóvenes universitarios

En este estudio se pretende indagar la posible relación de las variables sexo y género con el comportamiento sexual en personas universitarias. Para ello se aplicaron a 306 estudiantes universitarios tres cuestionarios: el Inventario de Roles Sexuales de Bem (BSRI, 1974), para operativizar la variable género, y otros dos, elaborados a partir del Inventario Sexual utilizado por Hsu et al. (1994), sobre conductas y fantasías sexuales. Se analizaron las propiedades estructurales y psicométricas de los cuestionarios, obteniéndose óptimos resultados, en concordancia con los referidos en la literatura. Se confirma la relación existente entre el género (masculino, femenino, andrógino e indiferenciado), el sexo (hombre-mujer) y los comportamientos sexuales, si bien esta relación es inferior a la esperada. Se señalan algunas limitaciones del estudio y se plantean propuestas para la investigación futura

Strategies to mitigate the impact of shifting from a milk and forage regimen to a high-concentrate diet in intensively reared beef cattle

El cebo de terneros en España se lleva a cabo mediante el aporte de dietas con un elevado porcentaje de concentrado en la ración y una mínima parte de forraje, con el fin de lograr un elevado crecimiento diario y acortar el ciclo de producción. Estas dietas son altamente fermentables en el rumen y pueden causar acidosis ruminal. El período de transición de los terneros a las dietas de cebo intensivo es una fase crítica, ya que habitualmente estos animales se destetan de forma abrupta e inmediatamente entran a cebadero. En el contexto de la presente tesis doctoral, se diseñaron tres experimentos con el fin de estudiar la efectividad de algunas estrategias para reducir el riesgo de padecer acidosis ruminal durante el período de transición de terneros de pasteros al cebo intensivo.El Experimento 1 se llevó a cabo para determinar el manejo alimentario previo al destete más adecuado (Leche, Leche+Heno o Leche+Pienso), en términos de desarrollo del rumen, rendimiento animal y rentabilidad económica, para terneros que son destetados de forma abrupta e inmediatamente introducidos al sistema de cebo intensivo.Teniendo en cuenta todo el ciclo de producción, la suplementación previa al destete con concentrado es la opción más interesante para los terneros de carne que van a entrar a cebadero ya que promovió una mayor adapatación del rumen y un mayor margen económico. El objetivo del Experimento 2 fue determinar el efecto de la sustitución parcial de la cebada por maíz y pulpa de remolacha en la dieta de adaptación de los terneros durante la transición a una ración de cebo intensivo sobre la ingestión y el rendimiento de los animales, la fermentación ruminal y la composición microbiana del rumen. El análisis de todas variables registradas denotó una falta de efecto tanto en los rendimientos, como en la fermentación ruminal y poblaciones microbianas. Por lo tanto, El uso de cebada o maíz como cereal predominante, o la inclusión de pulpa de remolacha, no tienen ningún efecto sobre la fermentación ruminal o las poblaciones microbianas en los terneros de engorde, y su uso dependerá del precio o la disponibilidad en el mercado de estos ingredientes.En el Experimento 3 se evaluó el efecto de la inclusión de una mezcla (65:35) de taninos de castaño y quebracho o de ácidos grasos de cadea media, como moduladores de la fermentación ruminal en la dieta de adaptación de terneros de carne, sobre la ingesta de alimento, el rendimiento animal, la fermentación ruminal y la composición microbiana del rumen. A las dosis utilizadas en el presente experimento, los taninos (20g/kg) y los AGCM (6g/kg) no ejercieron ningún efecto sobre la ingesta, el rendimiento animal, la fermentación ruminal y la población microbiana.<br /

El uso de los medios de comunicación de Twitter, sobre el coronavirus.

pre-print2827 K

Raising the Efficiency Limit of the GaAs-based Intermediate Band Solar Cell Through the Implementation of a Mololithic Tandem with an AlGaAs top Cell.

The high efficiency limit of the intermediate band solar cell (IBSC) corresponds to the case of using as intermediate band (IB) host material a semiconductor with gap in the range of 2 eV. Traditional photovoltaic materials, such as Si and GaAs, are not appropriate to produce IB devices because their gaps are too narrow. To overcome this problem, we propose the implementation of a multi-junction device consisting of an IBSC combined with a single gap cell. We calculate the efficiency limits using the detailed balance model and conclude that they are very high (> 60% under maximum concentration) for any fundamental bandgap from 0.7 to 3.6 eV in the IBSC inserted in the tandem. In particular, the two-terminal tandem of a GaAs-based IBSC current matched to an optimized AlGaAs top cell has an efficiency limit as high as 64%

<i>Phaeocystis globosa</i> Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair

Phaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER). Among them is a gene coding for a bimodular protein consisting of an N-terminal Polβ-like core fused to a C-terminal domain (PgVPolX), which shows homology with NAD+-dependent DNA ligases. Analysis of the biochemical features of the purified enzyme revealed that PgVPolX is a multifunctional protein that could act as a “Swiss army knife” enzyme during BER since it is endowed with: 1) a template-directed DNA polymerization activity, preferentially acting on DNA structures containing gaps; 2) 5′-deoxyribose-5-phosphate (dRP) and abasic (AP) site lyase activities; and 3) an NAD+-dependent DNA ligase activity. We show how the three activities act in concert to efficiently repair BER intermediates, leading us to suggest that PgVPolX may constitute, together with the viral AP-endonuclease, a BER pathway. This is the first time that this type of protein fusion has been demonstrated to be functional