Current perspectives of the signaling pathways directing neural crest induction.

The neural crest is a migratory population of embryonic cells with a tremendous potential to differentiate and contribute to nearly every organ system in the adult body. Over the past two decades, an incredible amount of research has given us a reasonable understanding of how these cells are generated. Neural crest induction involves the combinatorial input of multiple signaling pathways and transcription factors, and is thought to occur in two phases from gastrulation to neurulation. In the first phase, FGF and Wnt signaling induce NC progenitors at the border of the neural plate, activating the expression of members of the Msx, Pax, and Zic families, among others. In the second phase, BMP, Wnt, and Notch signaling maintain these progenitors and bring about the expression of definitive NC markers including Snail2, FoxD3, and Sox9/10. In recent years, additional signaling molecules and modulators of these pathways have been uncovered, creating an increasingly complex regulatory network. In this work, we provide a comprehensive review of the major signaling pathways that participate in neural crest induction, with a focus on recent developments and current perspectives. We provide a simplified model of early neural crest development and stress similarities and differences between four major model organisms: Xenopus, chick, zebrafish, and mouse

Neural networks and spectra feature selection for retrival of hot gases temperature profiles

Proceeding of: International Conference on Computational Intelligence for Modelling, Control and Automation, 2005 and International Conference on Intelligent Agents, Web Technologies and Internet Commerce, Vienna, Austria 28-30 Nov. 2005Neural networks appear to be a promising tool to solve the so-called inverse problems focused to obtain a retrieval of certain physical properties related to the radiative transference of energy. In this paper the capability of neural networks to retrieve the temperature profile in a combustion environment is proposed. Temperature profile retrieval will be obtained from the measurement of the spectral distribution of energy radiated by the hot gases (combustion products) at wavelengths corresponding to the infrared region. High spectral resolution is usually needed to gain a certain accuracy in the retrieval process. However, this great amount of information makes mandatory a reduction of the dimensionality of the problem. In this sense a careful selection of wavelengths in the spectrum must be performed. With this purpose principal component analysis technique is used to automatically determine those wavelengths in the spectrum that carry relevant information on temperature distribution. A multilayer perceptron will be trained with the different energies associated to the selected wavelengths. The results presented show that multilayer perceptron combined with principal component analysis is a suitable alternative in this field.Publicad

Solid flow drives surface nanopatterning by ion-beam irradiation

Ion Beam Sputtering (IBS) is known to produce surface nanopatterns over macroscopic areas on a wide range of materials. However, in spite of the technological potential of this route to nanostructuring, the physical process by which these surfaces self-organize remains poorly under- stood. We have performed detailed experiments of IBS on Si substrates that validate dynamical and morphological predictions from a hydrodynamic description of the phenomenon. Our results elucidate flow of a nanoscopically thin and highly viscous surface layer, driven by the stress created by the ion-beam, as a description of the system. This type of slow relaxation is akin to flow of macroscopic solids like glaciers or lead pipes, that is driven by defect dynamics.Comment: 12 pages, 4 figure

Mesenchymal stem cells and their use as cell replacement therapy and disease modelling tool.

Mesenchymal stem cells (MSCs) from adult somatic tissues may differentiate in vitro and in vivo into multiple mesodermal tissues including bone, cartilage, adipose tissue, tendon, ligament or even muscle. MSCs preferentially home to damaged tissues where they exert their therapeutic potential. A striking feature of the MSCs is their low inherent immunogenicity as they induce little, if any, proliferation of allogeneic lymphocytes and antigen-presenting cells. Instead, MSCs appear to be immunosuppressive in vitro. Their multilineage differentiation potential coupled to their immuno-privileged properties is being exploited worldwide for both autologous and allogeneic cell replacement strategies. Here, we introduce the readers to the biology of MSCs and the mechanisms underlying immune tolerance. We then outline potential cell replacement strategies and clinical applications based on the MSCs immunological properties. Ongoing clinical trials for graft-versus-host-disease, haematopoietic recovery after co-transplantation of MSCs along with haematopoietic stem cells and tissue repair are discussed. Finally, we review the emerging area based on the use of MSCs as a target cell subset for either spontaneous or induced neoplastic transformation and, for modelling non-haematological mesenchymal cancers such as sarcomas

Thermal diagnostic of the Optical Window on board LISA Pathfinder

Vacuum conditions inside the LTP Gravitational Reference Sensor must comply with rather demanding requirements. The Optical Window (OW) is an interface which seals the vacuum enclosure and, at the same time, lets the laser beam go through for interferometric Metrology with the test masses. The OW is a plane-parallel plate clamped in a Titanium flange, and is considerably sensitive to thermal and stress fluctuations. It is critical for the required precision measurements, hence its temperature will be carefully monitored in flight. This paper reports on the results of a series of OW characterisation laboratory runs, intended to study its response to selected thermal signals, as well as their fit to numerical models, and the meaning of the latter. We find that a single pole ARMA transfer function provides a consistent approximation to the OW response to thermal excitations, and derive a relationship with the physical processes taking place in the OW. We also show how system noise reduction can be accomplished by means of that transfer function.Comment: 20 pages, 14 figures; accepted for publication in Class. Quantum Gra

The computer in a roofing slate quarry

[Resumen] Sencillas configuraciones informáticas en base a ordenadores personales con programas comerciales de modelización y diseño asistido por ordenador, facilitan el reiterativo trabajo de planificación minera de una explotación de pizarra a cielo abierto. Su utilización es aplicable a tres fases del proyecto minero: Modelización del yacimiento (investigación), proyecto de explotación (viabilidad) y labores mineras (ejecución).[Abstract] Simple integrated systems based on personal computers and cornmercial programmes about modelling and CAD, make easy the reiterative work on exploitation planning in roofing slate quarries. We can use this systems in the three phases of the mining project: bed modelling (Research), exploitation (Viability)wand mining (Performance)

Linear programming of a slate quarry

[Abstract] A new exploitation of roofing slate has several possibilities of marketing depending on the sizes of the pieces that it makes. Three sizes has been chosen among aH the possibilities in base of the conditions and the production of the rock in the quarry, the marketing limitation and the final price of the producto We've procces aH these data to obtain the optimun output, with the simplex algorithm. The objective function (week invoicing) and the restrictions of the problem are created in canonical and standard formo Optimun solution has been obtained among aH the basic and practical ones, using the graphic and the analytic method. Sorne conclusions come from the algorithm matrix about the outputs for each marketing option

Pre-Calculated Duty Cycle Control Implemented in FPGA for Power Factor Correction

A power factor correction (PFC) technique based on pre-calculated duty cycle values is presented in this paper. In this method the duty ratios for half a line period are calculated in advance and stored in a memory. By synchronizing the memory with the line, near unity power factors can be achieved in a specific operating point. The main advantage of this technique is that neither current measurement nor current loop are needed. To obtain stable output voltages a voltage loop is included. A boost converter prototype controlled by an FPGA evaluation board has been implemented in order to verify the functionality of the proposed method. Both the simulation and experimental results show that near unity power factor can be achieved with this PFC strategy