Direct observation of significant hot carrier cooling suppression in a two-dimensional silicon phononic crystal

Finding hot carrier cooling suppression in new material structures is fundamentally important for developing promising technological applications. These phenomenona have not been reported for crystalline silicon phononic crystals. Herein, we experimentally design two-dimensional (2D) silicon samples consisting of airy hole arrays in a crystalline silicon matrix. For reference, the determined hot carrier cooling times were 0.45 ps and 0.37 ps, respectively, at probe wavelengths of 1080 nm and 1100 nm. Surprisingly, when the 2D structured silicon possessed the properties of a phononic crystal, significant suppression of hot carrier cooling was observed. In these cases, the observed hot carrier cooling times were as long as 15.9 ps and 10.7 ps at probe wavelengths of 1080 nm and 1100 nm, respectively, indicating prolongation by orders of magnitude. This remarkable enhancement was also observed with other probe wavelengths. The present work presents experimental evidence for hot carrier cooling suppression in 2D silicon phononic crystals and opens opportunities for promising applications

Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)

The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat

Nature-Based Solutions in “Forest–Wetland” Spatial Planning Strategies to Promote Sustainable City Development in Tianjin, China

Nature-based solutions are some of the most effective strategies to promote sustainable city development; however, existing research on NbS is mostly comprised of single variable studies rather than multiple variables. The purpose of this study was to explore the possibility of extending the NbS of a single variable to two variables for the better development of sustainable cities. Both forestation and wetland restoration are regarded as NbS for sustainable city development. The research approach of “forest–wetland” NbS was proposed and centers on the process and core issues of traditional NbS. Taking Tianjin as an example, the spatial patterns of forests and wetlands, correlation between the spatial distribution of forests and wetlands, and spatial correlation between the areas of forest growth and wetland growth within a certain distance in different years were studied using a spatial distribution pattern analysis, geographic concentration analysis, kernel density estimation and spatial autocorrelation analysis. Based on the core issues of NbS and the above spatial analysis, a “forest–wetland” spatial planning strategy was formulated. The main conclusions are as follows: forest and wetland were negatively correlated in the whole area of Tianjin, forest resources w mainly located in north, while wetland resources were mainly located in south. Compared with forests, the spatial distribution of wetlands in Tianjin was more balanced. There exist synergy and trade-offs between forest and wetland area under certain circumstances. Growth of forests was positively correlated with the growth of wetlands, within a distance of 0–400 m from 2000 to 2010, and within a distance of 0–600 m from 2010 to 2020. An increase in forest area will lead to an increase in evaporation, which in turn will hinder the growth of wetlands in Tianjin. Forest–wetland ecological network could promote synergistic between forest and wetland, and grey infrastructure to reduce potential trade-off between forest and wetland

Enhanced hydrogen evolution from CuOx-C/TiO2 with multiple electron transport pathways.

Titanium dioxide nanoparticles co-modified with CuOx (0≤x≤2) and carbonaceous materials were prepared with a simple hydrolysis and photo-reduction method for photocatalytic hydrogen generation. SEM/TEM and XPS analysis indicated that the carbonaceous materials were mostly coated on the TiO2 surface and clearly revealed that the Cu species exhibited multivalence states, existing as CuOx (0≤x≤2). The optimal catalyst showed a 56-fold enhanced hydrogen evolution rate compared with that of the pure C/TiO2 catalyst. Further, an intensive multiple electron transfer effect originating from CuOx and the carbonaceous materials is proposed to be responsible for the elevated photoactivity. CuOx species serve as electron donors facilitating charge carrier transfer and proton reduction sites. The carbonaceous materials function as the "bridge" that transfers the electrons of TiO2 to the CuOx species, which provides a new route for electron transfer and reinforces the effect of CuOx as a co-catalyst. In this study, the CuOx and C co-modified TiO2 catalyst was prepared with multiple electron transport pathways and enhanced hydrogen production evolution, which provides a deep understanding for the design of co-catalyst-based photocatalysts

CRISPR/Cas9: A powerful tool for crop genome editing

The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA (usually about 20 nucleotides) that is complementary to a target gene or locus and is anchored by a protospacer-adjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks (DSBs), which are subsequently repaired by non-homologous end joining (NHEJ) or homology-directed repair (HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions, whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement

The soft glumes of common wheat are sterile-lemmas as determined by the domestication gene Q

The Q gene in common wheat encodes an APETALA2 (AP2) transcription factor that causes the free threshing attribute. Wheat spikelets bearing several florets are subtended by a pair of soft glumes that allow free liberation of seeds. In wild species, the glumes are tough and rigid, making threshing difficult. However, the nature of these “soft glumes”, caused by the domestication allele Q is not clear. Here, we found that over expression of Q in common wheat leads to homeotic florets at glume positions. We provide phenotypic, microscopy, and marker genes evidence to demonstrate that the soft glumes of common wheat are in fact lemma-like organs, or so-called sterile-lemmas. By comparing the structures subtending spikelets in wheat and other crops such as rice and maize, we found that AP2 genes may play conserved functions in grasses by manipulating vestigial structures, such as floret-derived soft glumes in wheat and empty glumes in rice. Conversion of these seemingly vegetative organs to reproductive organs may be useful in yield improvement of crop species. Keywords: Floret development, Spike morphology, Sterile lemma, Whea