Inteligencias múltiples en educación preescolar: un análisis desde la perspectiva del Ministerio de Educación Pública, Centro de Educación Especial, Ministerio de Salud, Centros Privados y Escuela Científica Universidad Nacional Sede Región Brunca, en el cantón de Pérez Zeledón durante el II semestre del 2009 y I semestre del 2010

Seminario de Licenciatura presentado en la división de Educación Básica Centro de Investigación y Docencia en Educación Universidad Nacional Sede Región Brunca, para optar el grado de Licenciatura en Pedagogía con Énfasis en Educación PreescolarAnaliza las Inteligencias Múltiples desde la perspectiva del Ministerio de Educación Pública, Centro de Educación Especial, Ministerio de Salud, Centros Privados y Escuela Científica Universidad Nacional Sede Región Brunca, en el cantón de Pérez Zeledón, durante el II semestre del 2009 y el I semestre del 2010. En este seminario se determina el conocimiento que posee la docente sobre los elementos de las inteligencias múltiples, se identifica el papel de las inteligencias múltiples en la práctica pedagógica y también los factores sociales que inciden en relación a las inteligencias múltiples. Para la muestra se eligieron 2 aulas, 2 docentes y 2 hogares del ciclo de materno infantil de las instituciones públicas; 2 aulas, 2 docentes y 2 hogares del ciclo de transición igualmente de la institución pública. Además se trabajó con 2 aulas, 2 docentes y 4 hogares del Centro de Educación Especial, también se tomó en cuenta, 1 aula del centro privado, 1 docente y 4 hogares; del sector salud se escogieron 2 aulas, 2 docentes y 4 hogares y por último de la escuela científica 1 aula, 1 docente y 4 hogares. Es una investigación de tipo descriptivo con enfoque cualitativo. Los instrumentos utilizados fueron la entrevista aplicada a docentes y padres de familia además de una observación participante en las aulas de preescolar.It analyzes the Multiple Intelligences from the perspective of the Ministry of Public Education, Special Education Center, Ministry of Health, Private Centers and Science School National University Headquarters Brunca Region, in the canton of Perez Zeledon, during the second semester of 2009 and the first semester of 2010. In this seminar the knowledge that the teacher has about the elements of multiple intelligences is determined, the role of multiple intelligences in the pedagogical practice is identified and also the social factors that have an impact on multiple intelligences. For the sample, 2 classrooms, 2 teachers and 2 homes were chosen from the mother-child cycle of public institutions; 2 classrooms, 2 teachers and 2 homes from the transition cycle were also chosen from the public institution. In addition, we worked with 2 classrooms, 2 teachers and 4 homes from the Special Education Center, 1 classroom from the private center, 1 teacher and 4 homes; from the health sector we chose 2 classrooms, 2 teachers and 4 homes and finally from the science school 1 classroom, 1 teacher and 4 homes. It is a descriptive type of research with a qualitative approach. The instruments used were the interview applied to teachers and parents in addition to a participant observation in the preschool classrooms.Universidad Nacional, Costa RicaSede Regional Brunca, Campus Pérez Zeledó

Alternative and canonical NF-kB pathways DNA-binding hierarchies networks define Hodgkin lymphoma and Non-Hodgkin diffuse large B Cell lymphoma respectively

Purpose: Despite considerable evidence that supports the NF-kB role in the immune system and lymphomagenesis, it is unclear whether specific NF-kB dimers control a particular set of genes that account for their biological functions. Our previous work showed that Hodgkin Lymphoma (HL) is unique, among germinal center (GC)-derived lymphomas, with respect to its dependency on Rel-B to survive. In contrast, diffuse large B-Cell lymphoma (DLBCL) including both Activated B-Cell-Like and Germinal Center B-Cell-Like, requires cREL and Rel-A to survive and it is not affected by Rel-B depletion. These findings highlighted the activity of specific NF-kB subunits in different GC-derived lymphomas. Methods: Sequenced chromatin immunoprecipitated DNA fragments (ChIP-Seq) analysis revealed an extensive NF-kB DNA-binding network in DLBCL and HL. The ChIP-Seq data was merged with microarray analysis following the Rel-A, Rel-B or cRel knockdown to determine effectively regulated genes. Results: Downstream target analysis showed enrichment for cell cycle control, among other signatures. Rel-B and cRel controlled different genes within the same signature in HL and DLBCL, respectively. BCL2 was exclusively controlled by Rel-B in HL. Both mRNA and protein levels decreased following Rel-B depletion meanwhile there was no change upon cRel knock-down. BCL2 exogenous expression partially rescued the death induced by decreased Rel-B in HL cells. Conclusion: The Rel-B hierarchical network defined HL and the cRel hierarchical network characterized DLBCL. Each Rel member performs specific functions in distinct GC-derived lymphomas. This result should be considered for the development of targeted therapies that are aimed to selectively inhibit individual NF-kB dimers.Fil: Gamboa Cedeño, Angélica María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; ArgentinaFil: Castillo, Mariángeles. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; ArgentinaFil: Wenming, Xiao. National Institutes of Health; Estados UnidosFil: Waldmann, Thomas. National Institutes of Health; Estados UnidosFil: Ranuncolo, Stella Maris. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; Argentin

Apoptotic regulator BCL-2 blockade aS a potential therapy in classical Hodgkin Lymphoma

The challenge in classical Hodgkin Lymphoma (cHL) management is the 30–40% of refractory/relapsed cases. Aims: The aim of this work was to determine whether NIK and BCL-2 could be useful as prognosis biomarkers in cHL. In addition, we evaluated BCL-2 as a directed-therapy in cHL cell lines using venetoclax. Main methods: We evaluated NIK and BCL-2 expression in 112 untreated cHL patients' lymph-node biopsies by immunohistochemistry. cHL cell lines were treated with venetoclax alone or combined with vincristine or doxorubicin. Cell viability, metabolic activity and cell death were analyzed by trypan-blue exclusion method, MTS assay and FDA/IP staining respectively. Key findings: No correlation between NIK or BCL-2 expression and the majority of the clinical parameters was found. Patients with ≥60% BCL-2+ HRS-cells had a shorter disease-free survival (DFS) and overall survival (OS) (p = 0.002, p = 0.02 respectively). A decision tree analysis, in a 30 patients subgroup, showed that patients with <60% NIK+ HRS-cells but with ≥60% BCL-2+ HRS-cells had a worse outcome in terms of DFS and OS. These parameters performed better as prognosis indicators as compared to the diagnosis bone marrow status. Human cHL cell lines U-H01, KM-H2, L1236, SUPHD1, L540 showed sensitivity to venetoclax. The co-treatment effect of venetoclax and vincristine or doxorubicin on cell viability was diverse depending on the cell line evaluated. Significance: BCL-2 should be considered as a prognosis biomarker as well as a potential new therapeutic target in cHL. We report for the first time the cytotoxic effect of venetoclax in human cHL cell lines.Fil: Gamboa Cedeño, Angélica María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional e Ingeniería Biomédica - Hospital Italiano. Instituto de Medicina Traslacional e Ingeniería Biomédica.- Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional e Ingeniería Biomédica; ArgentinaFil: Díaz, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Cristaldo, Nancy. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Otero, Victoria. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Schutz, Natalia. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Fantl, Dorotea. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Cugliari, María Silvana. Universidad de Buenos Aires; ArgentinaFil: Zerga, Marta Elisa. Universidad de Buenos Aires; ArgentinaFil: Rojas Bilbao, Érica. Universidad de Buenos Aires; ArgentinaFil: Jauk, Federico. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Garcia Rivello, Hernan Jorge. Instituto Universidad Escuela de Medicina del Hospital Italiano; ArgentinaFil: Núñez, Myriam Carmen. Universidad de Buenos Aires; ArgentinaFil: Ranuncolo, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional e Ingeniería Biomédica - Hospital Italiano. Instituto de Medicina Traslacional e Ingeniería Biomédica.- Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional e Ingeniería Biomédica; Argentin