Multiple Transmitter Receptors in Regions and Layers of the Human Cerebral Cortex

We measured the densities (fmol/mg protein) of 15 different receptors of various transmitter systems in the supragranular, granular and infragranular strata of 44 areas of visual, somatosensory, auditory and multimodal association systems of the human cerebral cortex. Receptor densities were obtained after labeling of the receptors using quantitative in vitro receptor autoradiography in human postmortem brains. The mean density of each receptor type over all cortical layers and of each of the three major strata varies between cortical regions. In a single cortical area, the multi-receptor fingerprints of its strata (i.e., polar plots, each visualizing the densities of multiple different receptor types in supragranular, granular or infragranular layers of the same cortical area) differ in shape and size indicating regional and laminar specific balances between the receptors. Furthermore, the three strata are clearly segregated into well definable clusters by their receptor fingerprints. Fingerprints of different cortical areas systematically vary between functional networks, and with the hierarchical levels within sensory systems. Primary sensory areas are clearly separated from all other cortical areas particularly by their very high muscarinic M2 and nicotinic α4β2 receptor densities, and to a lesser degree also by noradrenergic α2 and serotonergic 5-HT2 receptors. Early visual areas of the dorsal and ventral streams are segregated by their multi-receptor fingerprints. The results are discussed on the background of functional segregation, cortical hierarchies, microstructural types, and the horizontal (layers) and vertical (columns) organization in the cerebral cortex. We conclude that a cortical column is composed of segments, which can be assigned to the cortical strata. The segments differ by their patterns of multi-receptor balances, indicating different layer-specific signal processing mechanisms. Additionally, the differences between the strata-and area-specific fingerprints of the 44 areas reflect the segregation of the cerebral cortex into functionally and topographically definable groups of cortical areas (visual, auditory, somatosensory, limbic, motor), and reveals their hierarchical position (primary and unimodal (early) sensory to higher sensory and finally to multimodal association areas).HighlightsDensities of transmitter receptors vary between areas of human cerebral cortex.Multi-receptor fingerprints segregate cortical layers.The densities of all examined receptor types together reach highest values in the supragranular stratum of all areas.The lowest values are found in the infragranular stratum.Multi-receptor fingerprints of entire areas and their layers segregate functional systemsCortical types (primary sensory, motor, multimodal association) differ in their receptor fingerprints

Creating hope in dystopia: Utopia as Method as social pedagogy in early childhood studies

Those who choose to engage with the academic world of early childhood are frequently caught between encouraging students to advocate for children and contribute to the construction of a good life and navigating the regulatory frameworks that shape future practice. In short, we must prepare students for the highly skilled work of supporting people who live their lives in day-to-day actions that are underpaid, under-resourced and overlooked. Those who prepare students for this reality are tasked with developing programmes that both instil hope and pragmatism that will sustain them when faced with these everyday realities. This article outlines how the authors addressed this through an adapted use of Utopia as Method in a module on an early childhood degree. By following its distinct modes, students are guided to position themselves not as passive observants of a childhood that is socially constructed around them, but as social and political actors engaged with making human beings human. Among other issues the article evaluates the intersection between social pedagogy, utopia and the future of early childhood. Based on explorations undertaken for this article, we argue that the imaginative reconstruction of childhood through higher education is at ease with the values and purpose of social pedagogy. We reflect that, while the method employed as part of a module was useful in terms of personal development and future-oriented practice, the need to include children’s voices is yet to be developed

Cosmic Evolution of Black Holes and Spheroids. IV. The BH Mass - Spheroid Luminosity Relation

From high-resolution images of 23 Seyfert-1 galaxies at z=0.36 and z=0.57 obtained with the Near Infrared Camera and Multi-Object Spectrometer on board the Hubble Space Telescope (HST), we determine host-galaxy morphology, nuclear luminosity, total host-galaxy luminosity and spheroid luminosity. Keck spectroscopy is used to estimate black hole mass (M_BH). We study the cosmic evolution of the M_BH-spheroid luminosity (L_sph) relation. In combination with our previous work, totaling 40 Seyfert-1 galaxies, the covered range in BH mass is substantially increased, allowing us to determine for the first time intrinsic scatter and correct evolutionary trends for selection effects. We re-analyze archival HST images of 19 local reverberation-mapped active galaxies to match the procedure adopted at intermediate redshift. Correcting spheroid luminosity for passive luminosity evolution and taking into account selection effects, we determine that at fixed present-day V-band spheroid luminosity, M_BH/L_sph \propto (1+z)^(2.8+/-1.2). When including a sample of 44 quasars out to z=4.5 taken from the literature, with luminosity and BH mass corrected to a self-consistent calibration, we extend the BH mass range to over two orders of magnitude, resulting in M_BH/L_sph \propto (1+z)^(1.4+/-0.2). The intrinsic scatter of the relation, assumed constant with redshift, is 0.3+/-0.1 dex (<0.6 dex at 95% CL). The evolutionary trend suggests that BH growth precedes spheroid assembly. Interestingly, the M_BH-total host-galaxy luminosity relation is apparently non-evolving. It hints at either a more fundamental relation or that the spheroid grows by a redistribution of stars. However, the high-z sample does not follow this relation, indicating that major mergers may play the dominant role in growing spheroids above z~1.Comment: 39 pages, 11 figures. Accepted for publication in the Astrophysical Journa

Deep Chandra Monitoring Observations of NGC 4278 : Catalog of Source Properties

We present the properties of the discrete X-ray sources detected in our monitoring program of the globular cluster (GC) rich elliptical galaxy, NGC 4278, observed with Chandra ACIS-S in six separate pointings, resulting in a co-added exposure of 458 ks. From this deep observation, 236 sources have been detected within the region overlapped by all observations, 180 of which lie within the D 25 ellipse of the galaxy. These 236 sources range in L X from 3.5 × 1036 erg s-1 (with 3σ upper limit 1 × 1038 erg s-1. From X-ray source photometry, it has been determined that the majority of the 236 point sources that have well-constrained colors have values that are consistent with typical low-mass X-ray binary spectra, with 29 of these sources expected to be background objects from the log N-log S relation. There are 103 sources in this population that exhibit long-term variability, indicating that they are accreting compact objects. Three of these sources have been identified as transient candidates, with a further three possible transients. Spectral variations have also been identified in the majority of the source population, where a diverse range of variability has been identified, indicating that there are many different source classes located within this galaxy.Peer reviewe

The natural axis of transmitter receptor distribution in the human cerebral cortex

Transmitter receptors constitute a key component of the molecular machinery for intercellular communication in the brain. Recent efforts have mapped the density of diverse transmitter receptors across the human cerebral cortex with an unprecedented level of detail. Here, we distill these observations into key organizational principles. We demonstrate that receptor densities form a natural axis in the human cerebral cortex, reflecting decreases in differentiation at the level of laminar organization and a sensory-to-association axis at the functional level. Along this natural axis, key organizational principles are discerned: progressive molecular diversity (increase of the diversity of receptor density); excitation/inhibition (increase of the ratio of excitatory-to-inhibitory receptor density); and mirrored, orderly changes of the density of ionotropic and metabotropic receptors. The uncovered natural axis formed by the distribution of receptors aligns with the axis that is formed by other dimensions of cortical organization, such as the myelo- and cytoarchitectonic levels. Therefore, the uncovered natural axis constitutes a unifying organizational feature linking multiple dimensions of the cerebral cortex, thus bringing order to the heterogeneity of cortical organization

“When You Move You Have Fun”: Perceived Barriers, and Facilitators of Physical Activity From a Child's Perspective

In Northern Ireland (NI), many children do not meet the recommended levels of physical activity (PA). To reduce the prevalence of physical inactivity and associated health conditions, it is important to understand the influences on children's PA, which in turn has the potential to inform future intervention design. The purpose of this formative study was to examine the current views, barriers, facilitators, experiences, and perceptions of children in relation to PA in the classroom, school, and home environments, and to assess the acceptability of components for a school-based intervention. Write and draw tasks and semi-structured focus groups (n = 10) were conducted with 50 children aged 7–9 years (22 boys, 28 girls) from six primary schools. Focus groups were recorded, transcribed, and analyzed thematically. Pen profiles were constructed from the transcripts in a deductive manner and represent key emergent themes. Results indicated that children's perception and knowledge of PA was mainly structured and sport-based, while some referred to fun, play and health. Fun, social support and outdoor activity were identified as key facilitators. Barriers included parental restrictions, lack of time and space in the different environments. The acceptability of intervention components was examined, children recognized the potential benefits of additional movement in the classroom, but opinions differed on the sit-to-stand desks. Findings contribute to a more detailed understanding of children's perceptions of context specific PA, the barriers they face, in addition to factors that support them to lead a physically active lifestyle, which may inform future PA promotion strategies

A human approach to restructuring the education system: why schools in England need social pedagogy.

In this article, we contend that the current schools' system in England needs to be carefully reconsidered if we are to offer opportunities for success (in its broadest sense) to those whom our current, technocratic education system excludes. With a focus on social pedagogy and human-centred learning systems, we argue that continued modifications to the existing education system are no longer sufficient and that an ideology overhaul is needed before any significant positive progress can be made. To this end, we suggest various ways that schools might work towards developing healthier and more inclusive communities, built on the key social pedagogical foundations of positive relationships, democracy, inclusion, creativity and pedagogical love. We also make recommendations for an education system in which the teacher, as a highly trained professional, can enjoy a professional autonomy commensurate with their level of qualification. Finally, we dispel some of the myths that have prevented radical, community-focused change to date

Acetylcholine Neurotransmitter Receptor Densities in the Striatum of Hemiparkinsonian Rats Following Botulinum Neurotoxin-A Injection

Cholinergic neurotransmission has a pivotal function in the caudate-putamen, and is highly associated with the pathophysiology of Parkinson's disease. Here, we investigated long-term changes in the densities of the muscarinic receptor subtypes M1, M2, M3 (mAchRs) and the nicotinic receptor subtype α4β2 (nAchRs) in the striatum of the 6-OHDA-induced hemiparkinsonian (hemi-PD) rat model using quantitative in vitro receptor autoradiography. Hemi-PD rats exhibited an ipsilateral decrease in striatal mAchR densities between 6 and 16%. Moreover, a massive and constant decrease in striatal nAchR density by 57% was found. A second goal of the study was to disclose receptor-related mechanisms for the positive motor effect of intrastriatally injected Botulinum neurotoxin-A (BoNT-A) in hemi-PD rats in the apomorphine rotation test. Therefore, the effect of intrastriatally injected BoNT-A in control and hemi-PD rats on mAchR and nAchR densities was analyzed and compared to control animals or vehicle-injected hemi-PD rats. BoNT-A administration slightly reduced interhemispheric differences of mAchR and nAchR densities in hemi-PD rats. Importantly, the BoNT-A effect on striatal nAchRs significantly correlated with behavioral testing after apomorphine application. This study gives novel insights of 6-OHDA-induced effects on striatal mAchR and nAchR densities, and partly explains the therapeutic effect of BoNT-A in hemi-PD rats on a cellular level

Data management and wearables in older adults: A systematic review.

Background: Wearable trackers as research or clinical tools are increasingly used to support the care of older adults, due to their practicality in self-monitoring and potential to promote healthy lifestyle behaviours. However, limited understanding of appropriate data collection methods and analysis for different contexts still exists. Aim: To summarise evidence on wearable data generation and management in older adults, focusing on physical activity (PA),electrocardiogram (ECG), and vital signs monitoring. In addition to examine the accuracy and utility of incorporating wearable trackers into care of older people. Methods: A systematic search of CINAHL, MEDLINE, PubMed and a manual search were conducted. Twenty studies targeting wearable tracker use in older adults met inclusion criteria.Results: Methodological designs for data collection and analysis were heterogeneous with diverse definitions of wear and no-wear time, the number and type of valid days, and proprietary algorithms. Wearable trackers had adequate accuracy for measuring step counts, moderate to vigorous physical activity (MVPA), ECG and heart rate (HR), but notfor respiratory rate. Participants reported ease of use and had high-level adherence over daily long-term use.Moreover, wearable trackers encouraged users to increase their daily PA level and decrease waist circumference, facilitating atrial fibrillation (AF) diagnoses and predicting length of stay. Conclusion: Wearable trackers are multidimensional technologies offering a viable and promising approach for sustained and scaled monitoring of older people’s health. Framework and/or guidelines including standards for the design, data management and application of use specifically for older adults is required to enhance validity and reliability