Neurospora crassa qa-2 gene fragment.

Neurospora crassa qa-2 gene fragment

A solid-reagent dispenser for use in the azocoll protease assay (and other insoluble substrate analyses).

A solid-reagent dispenser for use in the azocoll protease assay (and other insoluble substrate analyses)

Alpha-particle emitting 213Bi-anti-EGFR immunoconjugates eradicate tumor cells independent of oxygenation

Hypoxia is a central problem in tumor treatment because hypoxic cells are less sensitive to chemo- and radiotherapy than normoxic cells. Radioresistance of hypoxic tumor cells is due to reduced sensitivity towards low Linear Energy Transfer (LET) radiation. High LET α-emitters are thought to eradicate tumor cells independent of cellular oxygenation. Therefore, the aim of this study was to demonstrate that the cell-bound α-particle emitting 213Bi immunoconjugates efficiently kill hypoxic just like normoxic CAL33 tumor cells. For that purpose CAL33 cells were incubated with 213Bianti- EGFR-MAb or irradiated with photons with a nominal energy of 6 MeV both under hypoxic and normoxic conditions. Oxygenation of cells was checked via the hypoxia-associated marker HIF-1α. Survival of cells was analysed using the clonogenic assay. Cell viability was monitored with the WST colorimetric assay. Results were evaluated statistically using a t-test and a Generalized Linear Mixed Model (GLMM). Survival and viability of CAL33 cells decreased both after incubation with increasing 213Bi-anti-EGFR-MAb activity concentrations (9.25 kBq/ml – 1.48 MBq/ml) and irradiation with increasing doses of photons (0.5 – 12 Gy). Following photon irradiation survival and viability of normoxic cells were significantly lower than those of hypoxic cells at all doses analysed. In contrast, cell death induced by 213Bianti- EGFR-MAb turned out to be independent of cellular oxygenation. These results demonstrate for the first time that α-particle emitting 213Bi-immunoconjugates eradicate hypoxic tumor cells as effective as normoxic cells. Therefore, 213Biradioimmunotherapy seems to be an appropriate strategy for treatment of hypoxic tumors.JRC.E.5-Nuclear chemistr

Group status drives majority and minority integration preferences

WOS:000300955100009 (Nº de Acesso Web of Science)“Prémio Científico ISCTE-IUL 2013”This research examined preferences for national-and campus-level assimilative and pluralistic policies among Black and White students under different contexts, as majority-and minority-group members. We targeted attitudes at two universities, one where 85% of the student body is White, and another where 76% of students are Black. The results revealed that when a group constituted the majority, its members generally preferred assimilationist policies, and when a group constituted the minority, its members generally preferred pluralistic policies. The results support a functional perspective: Both majority and minority groups seek to protect and enhance their collective identities

Accounting for fishing days without a fishing set in the CPUE standardization of yellowfin tuna in free schools for the EU purse seine fleet operating in the eastern Atlantic Ocean during the 1993-2018 period

The time series of EU purse seine fleet catches per unit effort (CPUE) of yellowfin tuna (YFT) from the Atlantic Ocean were standardized using an extension of the Delta-lognormal GLMM to three components. The aim was to depict the trend in abundance for adult YFT observed in free schools (FSC). The originality of this work relied on the inclusion of i) null sets, considered as presence of YFT FSC, ii) fishing days without set, considered as absence of FSC, iii) EU fishing agreement in the exclusive economic zones driving EU purse seine fleet presence in these areas, and iv) time spent by centroid cell by boat by day to constrain detectability. Standardized CPUE for FSC was thus defined as the product of the number of set (positive and null) by spatio-temporal strata, the proportion of sets with large YFT (>10 kg) and the catch per large YFT set. To detect strata without sets, all activities recorded in captain logbooks were used for the period 1993-2018. This new standardization approach, therefore, represents a significant advance over previous efforts, though there are a number of avenues for future progress.Versión del edito

On pancultural self-enhancement: well-adjusted Taiwanese self-enhance on personally valued traits

Taiwanese participants made better-than-average judgments on collectivistic and individualistic traits, evaluated the personal importance of those traits, and completed measures of psychological adjustment (depression, perceived stress, subjective well-being, and satisfaction with life). Replicating findings from other East Asian samples, participants self-enhanced (i.e., regarded the self as superior to peers) more on collectivistic than individualistic attributes and assigned higher personal importance to the former than the latter. Moreover, better adjusted participants manifested a stronger tendency to self-enhance on personally important attributes. These data are consistent with the view that self-enhancement is a universal human motive that is expressed tactically and at odds with the assertion that self-enhancement is a uniquely Western phenomenon. <br/

Repairing Socially Aggregated Ontologies Using Axiom Weakening

Ontologies represent principled, formalised descriptions of agents’ conceptualisations of a domain. For a community of agents, these descriptions may differ among agents. We propose an aggregative view of the integration of ontologies based on Judgement Aggregation (JA). Agents may vote on statements of the ontologies, and we aim at constructing a collective, integrated ontology, that reflects the individual conceptualisations as much as possible. As several results in JA show, many attractive and widely used aggregation procedures are prone to return inconsistent collective ontologies. We propose to solve the possible inconsistencies in the collective ontology by applying suitable weakenings of axioms that cause inconsistencies

Intergroup contact and social change: Implications of negative and positive contact for collective action in advantaged and disadvantaged groups

Previous research has shown that (1) positive intergroup contact with an advantaged group can discourage collective action among disadvantaged-group members and (2) positive intergroup contact can encourage advantaged-group members to take action on behalf of disadvantaged outgroups. Two studies investigated the effects of negative as well as positive intergroup contact. Study 1 (N = 482) found that negative but not positive contact with heterosexual people was associated with sexual-minority students’ engagement in collective action (via group identification and perceived discrimination). Among heterosexual students, positive and negative contact were associated with, respectively, more and less LGB activism. Study 2 (N = 1,469) found that only negative contact (via perceived discrimination) predicted LGBT students’ collective action intentions longitudinally while only positive contact predicted heterosexual/cisgender students’ LGBT activism. Implications for the relationship between intergroup contact, collective action, and social change are discussed