Baroclinic geostrophic adjustment in a rotating circular basin

Baroclinic geostrophic adjustment in a rotating circular basin is investigated in a laboratory study. The adjustment process consists of a linear phase before advective and dissipative effects dominate the response for longer time. This work describes in detail the hydrodynamics and energetics of the linear phase of the adjustment process of a two-layer fluid from an initial step height discontinuity in the density interface DeltaH to a final response consisting of both geostrophic and fluctuating components. For a forcing lengthscale r(f) equal to the basin radius R-0, the geostrophic component takes the form of a basin-scale double gyre while the fluctuating component is composed of baroclinic Kelvin and Poincare waves. The Burger number S=R/r(f) (R is the baroclinic Rossby radius of deformation) and the dimensionless forcing amplitude epsilon = DeltaH/H-1 (H-1 is the upper-layer depth) characterize the response of the adjustment process. In particular, comparisons between analytical solutions and laboratory measurements indicate that for time tau: 1 < tau < S-1 (tau is time scaled by the inertial period 2pi/f), the basin-scale double gyre is established, followed by a period where the double gyre is sustained, given by S-1 < tau < 2epsilon(-1) for a moderate forcing and S-1 < tau < tau(D) for a weak forcing (tau(D) is the dimensionless dissipation timescale due to Ekman damping). The analytical solution is used to calculate the energetics of the baroclinic geostrophic adjustment. The results are found to compare well with previous studies with partitioning of energy between the geostrophic and fluctuating components exhibiting a strong dependence on S. Finally, the outcomes of this study are considered in terms of their application to lakes influenced by the rotation of the Earth

Alternative Splicing in the Differentiation of Human Embryonic Stem Cells into Cardiac Precursors

The role of alternative splicing in self-renewal, pluripotency and tissue lineage specification of human embryonic stem cells (hESCs) is largely unknown. To better define these regulatory cues, we modified the H9 hESC line to allow selection of pluripotent hESCs by neomycin resistance and cardiac progenitors by puromycin resistance. Exon-level microarray expression data from undifferentiated hESCs and cardiac and neural precursors were used to identify splice isoforms with cardiac-restricted or common cardiac/neural differentiation expression patterns. Splice events for these groups corresponded to the pathways of cytoskeletal remodeling, RNA splicing, muscle specification, and cell cycle checkpoint control as well as genes with serine/threonine kinase and helicase activity. Using a new program named AltAnalyze (http://www.AltAnalyze.org), we identified novel changes in protein domain and microRNA binding site architecture that were predicted to affect protein function and expression. These included an enrichment of splice isoforms that oppose cell-cycle arrest in hESCs and that promote calcium signaling and cardiac development in cardiac precursors. By combining genome-wide predictions of alternative splicing with new functional annotations, our data suggest potential mechanisms that may influence lineage commitment and hESC maintenance at the level of specific splice isoforms and microRNA regulation

ISSN exercise & sport nutrition review: research & recommendations

Sports nutrition is a constantly evolving field with hundreds of research papers published annually. For this reason, keeping up to date with the literature is often difficult. This paper is a five year update of the sports nutrition review article published as the lead paper to launch the JISSN in 2004 and presents a well-referenced overview of the current state of the science related to how to optimize training and athletic performance through nutrition. More specifically, this paper provides an overview of: 1.) The definitional category of ergogenic aids and dietary supplements; 2.) How dietary supplements are legally regulated; 3.) How to evaluate the scientific merit of nutritional supplements; 4.) General nutritional strategies to optimize performance and enhance recovery; and, 5.) An overview of our current understanding of the ergogenic value of nutrition and dietary supplementation in regards to weight gain, weight loss, and performance enhancement. Our hope is that ISSN members and individuals interested in sports nutrition find this review useful in their daily practice and consultation with their clients