Improved distance determination to M51 from supernovae 2011dh and 2005cs

The appearance of two recent supernovae, SN 2011dh and 2005cs, both in M51, provides an opportunity to derive an improved distance to their host galaxy by combining the observations of both SNe. We apply the Expanding Photosphere Method to get the distance to M51 by fitting the data of these two SNe simultaneously. In order to correct for the effect of flux dilution, we use correction factors (zeta) appropriate for standard type II-P SNe atmospheres for 2005cs, but find zeta ~ 1 for the type IIb SN 2011dh, which may be due to the reduced H-content of its ejecta. The EPM analysis resulted in D_M51 = 8.4 +/- 0.7 Mpc. Based on this improved distance, we also re-analyze the HST observations of the proposed progenitor of SN 2011dh. We confirm that the object detected on the pre-explosion HST-images is unlikely to be a compact stellar cluster. In addition, its derived radius (~ 277$ R_sun) is too large for being the real (exploded) progenitor of SN 2011dh. The supernova-based distance, D = 8.4 Mpc, is in good agreement with other recent distance estimates to M51.Comment: 6 pages, 5 figures, accepted for publication in A&

Effect of X-Irradiation on Adenylate Cyclase Activity and Cyclic AMP Content of Primary Human Fibroblasts

Ionizing radiation provokes an increase of the cAMP level in several organs and body fluids. After reviewing the relevant literature we present the results of our own experiments on primary human fibroblasts. X-irradiation at doses of 0.5 and 2.5 Gy in vitro evoked a rapid and reversible increase of adenylate cyclase enzyme activity. A significant increase in cAMP level of these cells was also observed. Adenylate cyclase was usually localized basolaterally on the surface of unirradiated cells, while irradiation resulted in a modification of distribution, i.e., the enzyme activity also appeared in apical localization

Optical photometry and spectroscopy of the 1987A-like supernova 2009mw

We present optical photometric and spectroscopic observations of the 1987A-like supernova (SN) 2009mw. Our $BVRI$ and $g'r'i'z'$ photometry covers 167 days of evolution, including the rise to the light curve maximum, and ends just after the beginning of the linear tail phase. We compare the observational properties of SN 2009mw with those of other SNe belonging to the same subgroup, and find that it shows similarities to several objects. The physical parameters of the progenitor and the SN are estimated via hydrodynamical modelling, yielding an explosion energy of $1$ foe, a pre-SN mass of $19\,{\rm M_{\odot}}$, a progenitor radius as $30\,{\rm R_{\odot}}$ and a $^{56}$Ni mass as $0.062\,{\rm M_{\odot}}$. These values indicate that the progenitor of SN 2009mw was a blue supergiant star, similar to the progenitor of SN 1987A. We examine the host environment of SN 2009mw and find that it emerged from a population with slightly sub-solar metallicty.Comment: 11 pages, 12 figures, accepted for publication in MNRA

X-Irradiation-Induced Changes of the Prelysosomal and Lysosomal Compartments and Proteolysis in HT-29 Cells

As a consequence of external and internal ionizing radiation, lysosome-like bodies have been observed to increase both in size and number in some cell types. We investigated this process by morphological methods (electron microscopy, cationized ferritin uptake, acid phosphatase histochemistry, morphometry) in cultured HT-29 cells. In parallel with these studies, we measured the rate of protein degradation on the basis of 14C-valine release from prelabeled cellular proteins. We found that at 2 and 4 Gy doses of X-irradiation the volume of the vacuolar (probably lysosomal) compartment increased without detectable changes of acid phosphatase activity. A 2 Gy irradiation dose did not change protein degradation rate. However, 4 Gy caused a significant inhibition of 14C-valine release from prelabeled proteins. Our results indicate, that the radiation induced expansion of the lysosomal compartment is not necessarily accompanied by increased lytic activity of HT-29 cells

SN 2009ib: A Type II-P supernova with an unusually long plateau

We present optical and near-infrared photometry and spectroscopy of SN 2009ib, a Type II-P supernova in NGC 1559. This object has moderate brightness, similar to those of the intermediate-luminosity SNe 2008in and 2009N. Its plateau phase is unusually long, lasting for about 130 days after explosion. The spectra are similar to those of the subluminous SN 2002gd, with moderate expansion velocities. We es- timate the 56Ni mass produced as 0.046 ± 0.015 M⊙. We determine the distance to SN 2009ib using both the expanding photosphere method (EPM) and the standard candle method. We also apply EPM to SN 1986L, a type II-P SN that exploded in the same galaxy. Combining the results of different methods, we conclude the distance to NGC 1559 as D = 19.8 ± 3.0 Mpc. We examine archival, pre-explosion images of the field taken with the Hubble Space Telescope, and find a faint source at the position of the SN, which has a yellow colour ((V − I)0 = 0.85 mag). Assuming it is a single star, we estimate its initial mass as MZAMS = 20 M⊙. We also examine the possibility, that instead of the yellow source the progenitor of SN 2009ib is a red supergiant star too faint to be detected. In this case we estimate the upper limit for the initial zero-age main sequence mass of the progenitor to be ∼ 14 − 17 M⊙. In addition, we infer the physical properties of the progenitor at the explosion via hydrodynamical modelling of the observables, and estimate the total energy as ∼ 0.55 × 1051 erg, the pre-explosion radius as ∼ 400 R⊙, and the ejected envelope mass as ∼ 15 M⊙, which implies that the mass of the progenitor before explosion was ∼ 16.5 − 17 M

Extensive Spectroscopy and Photometry of the Type IIP Supernova 2013ej

We present extensive optical ($UBVRI$, $g'r'i'z'$, and open CCD) and near-infrared ($ZYJH$) photometry for the very nearby Type IIP SN ~2013ej extending from +1 to +461 days after shock breakout, estimated to be MJD $56496.9\pm0.3$. Substantial time series ultraviolet and optical spectroscopy obtained from +8 to +135 days are also presented. Considering well-observed SNe IIP from the literature, we derive $UBVRIJHK$ bolometric calibrations from $UBVRI$ and unfiltered measurements that potentially reach 2\% precision with a $B-V$ color-dependent correction. We observe moderately strong Si II $\lambda6355$ as early as +8 days. The photospheric velocity ($v_{\rm ph}$) is determined by modeling the spectra in the vicinity of Fe II $\lambda5169$ whenever observed, and interpolating at photometric epochs based on a semianalytic method. This gives $v_{\rm ph} = 4500\pm500$ km s$^{-1}$ at +50 days. We also observe spectral homogeneity of ultraviolet spectra at +10--12 days for SNe IIP, while variations are evident a week after explosion. Using the expanding photosphere method, from combined analysis of SN 2013ej and SN 2002ap, we estimate the distance to the host galaxy to be $9.0_{-0.6}^{+0.4}$ Mpc, consistent with distance estimates from other methods. Photometric and spectroscopic analysis during the plateau phase, which we estimated to be $94\pm7$ days long, yields an explosion energy of $0.9\pm0.3\times10^{51}$ ergs, a final pre-explosion progenitor mass of $15.2\pm4.2$~M$_\odot$ and a radius of $250\pm70$~R$_\odot$. We observe a broken exponential profile beyond +120 days, with a break point at +$183\pm16$ days. Measurements beyond this break time yield a $^{56}$Ni mass of $0.013\pm0.001$~M$_\odot$.Comment: 29 pages, 23 figures, 15 tables, Published in The Astrophisical Journa

Mass spectrometry imaging of cassette-dosed drugs for higher throughput pharmacokinetic and biodistribution analysis

Cassette dosing of compounds for preclinical drug plasma pharmacokinetic analysis has been shown to be a powerful strategy within the pharmaceutical industry for increasing throughput while decreasing the number of animals used. Presented here for the first time is data on the application of a cassette dosing strategy for label-free tissue distribution studies. The aim of the study was to image the spatial distribution of eight nonproprietary drugs (haloperidol, bufuralol, midazolam, clozapine, terfenadine, erlotinib, olanzapine, and moxifloxacin) in multiple tissues after oral and intravenous cassette dosing (four compounds per dose route). An array of mass spectrometry imaging technologies, including matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI), liquid extraction surface analysis tandem mass spectrometry (LESA-MS/MS), and desorption electrospray ionization mass spectrometry (DESI-MS) was used. Tissue analysis following intravenous and oral administration of discretely and cassette-dosed compounds demonstrated similar relative abundances across a range of tissues indicating that a cassette dosing approach was applicable. MALDI MSI was unsuccessful in detecting all of the target compounds; therefore, DESI MSI, a complementary mass spectrometry imaging technique, was used to detect additional target compounds. In addition, by adapting technology used for tissue profiling (LESA-MS/MS) low spatial resolution mass spectrometry imaging (∼1 mm) was possible for all targets across all tissues. This study exemplifies the power of multiplatform MSI analysis within a pharmaceutical research and development (R&D) environment. Furthermore, we have illustrated that the cassette dosing approach can be readily applied to provide combined, label-free pharmacokinetic and drug distribution data at an early stage of the drug discovery/development process while minimizing animal usage

Unique metabolites protect earthworms against plant polyphenols

All higher plants produce polyphenols, for defence against above-ground herbivory. These polyphenols also influence the soil micro- and macro-fauna that break down plant leaf litter. Polyphenols therefore indirectly affect the fluxes of soil nutrients and, ultimately, carbon turnover and ecosystem functioning in soils. It is unknown how earthworms, the major component of animal biomass in many soils, cope with high-polyphenol diets. Here, we show that earthworms possess a class of unique surface-active metabolites in their gut, which we term ‘drilodefensins’. These compounds counteract the inhibitory effects of polyphenols on earthworm gut enzymes, and high-polyphenol diets increase drilodefensin concentrations in both laboratory and field populations. This shows that drilodefensins protect earthworms from the harmful effects of ingested polyphenols. We have identified the key mechanism for adaptation to a dietary challenge in an animal group that has a major role in organic matter recycling in soils worldwide