NICU nurses' perceptions of nursing practice based on family-centered care

目的 : わが国では，1970年代終わりに新生児看護実践にfamily-centered careが取り入れられ始め，その後，family-centered developmental careの普及とともに，family-centeredであることに関心が向けられるようになった．しかし今日，NICU看護師が，自分自身のFCCに基づいた実践をどのように捉え，かつ，実践を困難にさせる理由をどのように捉えているかは十分明らかにされていない．そこで本研究では，FCCに基づいた看護実践に関するNICU看護師の認識を調査し，FCCを推進するための課題を考察することとした． 方法 : 無記名式の構成型質問紙調査を郵送法によって行った．対象者は日本新生児看護学会員674名とし，平成23年4月と平成24年5月に実施した．質問内容は，回答者の背景,FCCに基づいた看護実践，FCCに基づいた看護実践が困難な理由とした．分析は，基本統計量の算出，t検定，一元配置分散分析によって行った． 結果 : 340名から回答を得た(51.4%)．1)各基本概念の平均得点は，「心がけている」「実践している」とも，有意に「参加」が高く，「協働」が低かった．2)基本概念のいずれにおいても，「実践している」より「心がけている」の得点が有意に高かった．3)FCCに基づいた看護実践が困難な理由は，知識や実務の側面が上位を占め，最も多かったのは「医師，看護スタッフ・師長が，FCCの理念を実践化する方法を知らない」であった．4)FCCに基づいた看護実践が困難な理由は，年齢，総臨床経験年数NICU経験年数，他領域経験年数と関連した． 結論 : FCCの推進には，ガイドラインの作成および教育・訓練が重要な課題であることが考えられた．Objectives: Family-centered care was introduced into neonatal nursing practice in Japan at the end of the 1970s. It attracted much interest with the spread of family-centered development care. Today, however, what NICU nurses think about their practice based on FCC, and the reasons it is difficult to practice are not clearly understood. We therefore surveyed the perceptions of NICU nurses on nursing practice based on FCC, and discussed ways of promoting FCC. Methods: An anonymous structured question survey was sent by mail to 674 members of the Japan Academy of Neonatal Nursing in April 2011 and May 2012. Questions asked were on the respondents' background, nursing practice based on family-centered care, and reasons why practicing FCC was difficult. Data were descriptively analyzed and then a t - test and a one- way ANOVA were carried out. Results: 340 valid responses were received (51.4%). 1) Concerning practicing or being mindful of the basic concept of FCC, "participation" scored significantly highly, while the score for "collaboration" was low. 2) For all the basic concepts, the response "mindful" scored significantly higher than "practice". 3) As for the reasons why nursing practice based on FCC is difficult, most issues were related to knowledge and practice. The most frequent response was "doctors, nursing staff, and head nurses do not know how to practice the concept of FCC". 4) The difficulty of nursing practice based on FCC correlated with age and years of total clinical experience, NICU experience, and experience in other areas. Conclusion: Developing the Guidelines and providing education and training are considered necessary to promote nursing practice based on FCC.本研究は日本学術振興会科学研究費補助金基盤研究(B)22390430の助成を受けた研究の一部である

Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells

The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS

シェーグレン症候群の新規自己抗原であるREG 蛋白の唾液導管細胞における発現誘導にはIL-6/STAT 経路が重要である

The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS.博士（医学）・甲第641号・平成27年11月27日Copyright © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

「看護の将来を考える会」の活動報告

紀要委員会企画Special Articles　看護をとりまく環境の激的な変化に関する情報を得て、看護学部教員の有志が領域を超えて看護の将来について自由に語り合う場として、2017 年12 月から「看護の将来を考える会」の活動を開始した。毎月開催し、2018 年12 月で第11 回を数え、延べ115 人が参加した。活動実績と成果について「看護の将来考える会」の世話人として報告する

Genome-wide DNA methylation profiles in both precancerous conditions and clear cell renal cell carcinomas are correlated with malignant potential and patient outcome

To clarify genome-wide DNA methylation profiles during multistage renal carcinogenesis, bacterial artificial chromosome array-based methylated CpG island amplification (BAMCA) was performed. Non-cancerous renal cortex tissue obtained from patients with clear cell renal cell carcinomas (RCCs) (N) was at the precancerous stage where DNA hypomethylation and DNA hypermethylation on multiple bacterial artificial chromosome (BAC) clones were observed. By unsupervised hierarchical clustering analysis based on BAMCA data for their N, 51 patients with clear cell RCCs were clustered into two subclasses, Clusters AN (n = 46) and BN (n = 5). Clinicopathologically aggressive clear cell RCCs were accumulated in Cluster BN, and the overall survival rate of patients in Cluster BN was significantly lower than that of patients in Cluster AN. By unsupervised hierarchical clustering analysis based on BAMCA data for their RCCs, 51 patients were clustered into two subclasses, Clusters AT (n = 43) and BT (n = 8). Clinicopathologically aggressive clear cell RCCs were accumulated in Cluster BT, and the overall survival rate of patients in Cluster BT was significantly lower than that of patients in Cluster AT. Multivariate analysis revealed that belonging to Cluster BT was an independent predictor of recurrence. Cluster BN was completely included in Cluster BT, and the majority of the BAC clones that significantly discriminated Cluster BN from Cluster AN also discriminated Cluster BT from Cluster AT. In individual patients, DNA methylation status in N was basically inherited by the corresponding clear cell RCC. DNA methylation alterations in the precancerous stage may generate more malignant clear cell RCCs and determine patient outcome

特定行為研修における３年間の取り組み

紀要委員会企画Special Articles　2018 年に本学は看護師特定行為研修指定研修機関に指定され、2019 年から研修を開始した。３年間の研修で 14 名の研修生が、総合病院や訪問看護事業所等において適切な医療を提供するために、特定行為を実施する看護師を目指し、指定の講義・演習・実習を履修した。　本報告では、特定行為実施が望まれる社会的背景、医療現場で求められる特定行為、看護師が行う特定行為の意味、そして特定行為研修の今後の発展等について述べる

Characteristics of Training Center for Nurses Pertaining to Specified Medical Practice at Seirei Christopher University

P(論文)本学は2018 年に看護師特定行為研修指定研修機関に指定され、2019 年から研修を開始した。開講区分は「栄養及び水分管理に係る薬剤投与関連」の1 区分で、4 年間で16 名の修了者（見込み含む） を輩出した。2023 年度からは「在宅・慢性期領域パッケージ」が追加となる。本報告では、本学における特定行為研修の実際と特色についてまとめ、高度実践看護師育成に向けた今後の課題、方向性について述べる。紀要委員会企画Special Articlesdepartmental bulletin pape

Autoantibodies against matrix metalloproteinase-1 in patients with localized scleroderma

Background: Localized scleroderma (LSc) is characterized by cutaneous fibrosis and various autoantibodies. Objective: To determine the presence or levels of antibodies (Abs) against matrix metalloproteinase (MMP)-1 and their clinical relevance in LSc. Methods: Anti-MMP-1 Ab was examined by ELISA (Enzyme-Linked ImmunoSorbent Assay) and immunoblotting using human recombinant MMP-1. MMP-1 collagenase activity was determined using biotinylated collagen as substrate and the amount of cleaved biotinylated fragments of collagen by MMP-1 was measured by ELISA. Results: LSc patients exhibited significantly elevated IgG anti-MMP-1 Ab levels relative to normal controls at similar level of patients with systemic sclerosis (SSc). However, IgG anti-MMP-1 Ab levels were comparable among the 3 LSc subgroups: morphea, linear scleroderma, and generalized morphea. When absorbance values higher than the mean + 2S.D. of normal controls were considered positive, IgG or IgM anti-MMP-1 Ab was found in 46% and 49% of total LSc patients and SSc patients, respectively. Anti-MMP-1 Ab was detected most frequently in morphea patients (60%), followed by linear scleroderma patients (47%) and then generalized morphea patients (25%). LSc patients positive for IgG anti-MMP-1 Ab had elevated levels of IgG anti-single-stranded DNA Ab, IgG anti-nucleosome Ab, and shorter disease duration relative to those negative. The presence of anti-MMP-1 Ab in LSc patients was confirmed by immunoblotting. IgG isolated from LSc patients\u27 sera positive for IgG anti-MMP-1 Ab by ELISA inhibited MMP-1 collagenase activity. Conclusion: These results suggest that anti-MMP-1 autoantibody is a novel autoantibody in LSc. c 2008 Japanese Society for Investigative Dermatology

Hitomi (ASTRO-H) X-ray Astronomy Satellite

The Hitomi (ASTRO-H) mission is the sixth Japanese x-ray astronomy satellite developed by a large international collaboration, including Japan, USA, Canada, and Europe. The mission aimed to provide the highest energy resolution ever achieved at E  >  2  keV, using a microcalorimeter instrument, and to cover a wide energy range spanning four decades in energy from soft x-rays to gamma rays. After a successful launch on February 17, 2016, the spacecraft lost its function on March 26, 2016, but the commissioning phase for about a month provided valuable information on the onboard instruments and the spacecraft system, including astrophysical results obtained from first light observations. The paper describes the Hitomi (ASTRO-H) mission, its capabilities, the initial operation, and the instruments/spacecraft performances confirmed during the commissioning operations for about a month

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection