98 research outputs found
Phosphorylation-mediated unfolding of a KH domain regulates KSRP localization via 14-3-3 binding
The AU-rich element (ARE)-mediated mRNA-degradation activity of the RNA binding K-homology splicing regulator protein (KSRP) is regulated by phosphorylation of a serine within its N-terminal KH domain (KH1). In the cell, phosphorylation promotes the interaction of KSRP and 14-3-3ζ protein and impairs the ability of KSRP to promote the degradation of its RNA targets. Here we examine the molecular details of this mechanism. We report that phosphorylation leads to the unfolding of the structurally atypical and unstable KH1, creating a site for 14-3-3ζ binding. Using this site, 14-3-3ζ discriminates between phosphorylated and unphosphorylated KH1, driving the nuclear localization of KSRP. 14-3-3ζ âKH1 interaction regulates the mRNA-decay activity of KSRP by sequestering the protein in a separate functional pool. This study demonstrates how an mRNA-degradation pathway is connected to extracellular signaling networks through the reversible unfolding of a protein domain.European Molecular Biology Organization 240-2005Italian CIPE-200
Search for Neutral Heavy Leptons Produced in Z Decays
Weak isosinglet Neutral Heavy Leptons () have been searched for using data collected by the DELPHI detector corresponding to hadronic~Z decays at LEP1. Four separate searches have been performed, for short-lived production giving monojet or acollinear jet topologies, and for long-lived giving detectable secondary vertices or calorimeter clusters. No indication of the existence of these particles has been found, leading to an upper limit for the branching ratio Z of about at 95\% confidence level for masses between 3.5 and 50 GeV/. Outside this range the limit weakens rapidly with the mass. %Special emphasis has been given to the search for monojet--like topologies. One event %has passed the selection, in agreement with the expectation from the reaction: %. The results are also interpreted in terms of limits for the single production of excited neutrinos
Search for Lepton Flavour Number violating -Decays
A search for lepton flavour number violating decays in the channels \begin{center} ,\\ , \\ , \\ \end{center} using the DELPHI detector with data collected during the 1991--94 LEP runs, is described. No signal was found. Upper limits at 95\% confidence level for the respective branching fractions of , , and , were obtained
Search for neutral heavy leptons produced in decays
Weak isosinglet Neutral Heavy Leptons (Îœm) have been searched for using data collected by the DELPHI detector corresponding to 3.3 Ă 106 hadronic Z0 decays at LEP1. Four separate searches have been performed, for short-lived Îœm production giving monojet or acollinear jet topologies, and for long-lived Îœm giving detectable secondary vertices or calorimeter clusters. No indication of the existence of these particles has been found, leading to an upper limit for the branching ratio BR(Z0 â ÎœmÎœÌ) of about 1.3 Ă 10-6 at 95% confidence level for Îœm masses between 3.5 and 50 GeV/c2. Outside this range the limit weakens rapidly with the Îœm mass. The results are also interpreted in terms of limits for the single production of excited neutrinos. © Springer-Verlag 1997
Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6
International Consensus Statement on Rhinology and Allergy: Rhinosinusitis
Background: The 5 years since the publication of the first International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICARâRS) has witnessed foundational progress in our understanding and treatment of rhinologic disease. These advances are reflected within the more than 40 new topics covered within the ICARâRSâ2021 as well as updates to the original 140 topics. This executive summary consolidates the evidenceâbased findings of the document. Methods: ICARâRS presents over 180 topics in the forms of evidenceâbased reviews with recommendations (EBRRs), evidenceâbased reviews, and literature reviews. The highest grade structured recommendations of the EBRR sections are summarized in this executive summary. Results: ICARâRSâ2021 covers 22 topics regarding the medical management of RS, which are grade A/B and are presented in the executive summary. Additionally, 4 topics regarding the surgical management of RS are grade A/B and are presented in the executive summary. Finally, a comprehensive evidenceâbased management algorithm is provided. Conclusion: This ICARâRSâ2021 executive summary provides a compilation of the evidenceâbased recommendations for medical and surgical treatment of the most common forms of RS
High-Performance Capacitive Deionization of Copper Ions at Nanoporous ZnS-Decorated Carbon Felt
Extraction of dilute copper ions from wastewater is of great importance for simultaneous remediation of heavy-metal pollution and recovery of valuable metals. In this study, an efficient and selective method for copper recovery using capacitive deionization on ZnS-decorated carbon felt electrodes was developed and found suitable for use in HCl electrolytes. With the introduction of ZnS, the modified electrode exhibited a nanoporous structure with enhanced electrochemical capacitance, increased surface area and improved wettability. As a result, the copper ions were successfully removed via electrostatic interactions at low voltages of -0.2 V, in which the removal capacity of 27.4 mg.g(-1) is much better than the corresponding ones of FeS-decorated (18 mg.g(-1)) and pristine (12 mg.g(-1)) carbon felts. In addition to improved performance, enhanced adsorption-desorption stability and selectivity were observed during long-term tests in the presence of co-existing metallic ions and natural organic matter, offering promising potential as an alternative metal recycling and water purification technique. (C) 2019 The Electrochemical Society
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