Análisis comparativo de la tecnomorfología cerámica de los sitios de la cuenca media e inferior del Río Coronda

En este trabajo se presentan los resultados preliminares del estudio de la producción tecnológica cerámica elaborada por grupos cazadores-recolectores que habitaron sitios ubicados en la Cuenca Media e Inferior del río Coronda (provincia de Santa Fe). A partir del análisis macroscópico de los fragmentos recuperados se espera aportar a la comprensión de las elecciones tecnológicas cerámicas así como también a las diversas manifestaciones conductuales humanas que produjeron estos registros. El poder analizar registros que provienen de diferentes sitios comprendidos dentro de la misma zona permite obtener un panorama general respecto de las similitudes- diferencias en las elecciones realizadas para dar respuesta a las necesidades que plantea un ambiente particular.In this paper we present the first results about the study of pottery realized by hunters-gatherers that inhabited the sites of the Lower Basin of the Coronda river (province of Santa Fe). The macroscopic analysis of the sherds was done in order to know which elections were carry out about the pottery production and others behaviors associated with this activity. The analysis of this archaeological record that come from different sites in the zone allows us to have a general view about the similarities and differences in the choices made by people who lived in this particular environment in the past.Neste artigo, apresentamos resultados preliminares da produção tecnológica de cerâmica produzidas pelos caçadores-recolectores que habitavam locais no Médio e Baixo rio Bacia Coronda (Santa Fe). A partir da análise macroscópica dos fragmentos recuperados devem contribuir para a compreensão das escolhas de tecnologia cerâmica, bem como as diversas manifestações do comportamento humano que produziu esses registros. O poder de analisar os registros de sites diferentes que se encontram dentro da mesma área genera a posibilidade de ter uma visão geral sobre as semelhanças e as diferenças nas escolhas feitas para atender às necessidades decorrentes de um ambiente particular.Fil: Sartori, Julieta Isabel. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Humanidades y Artes; ArgentinaFil: Scuizzatto, Franca. Universidad Nacional de Rosario. Facultad de Humanidades y Artes; ArgentinaFil: Galligani, Paula Elisabet. Universidad Nacional de Rosario. Facultad de Humanidades y Artes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Toxic cyanobacteria strains isolated from blooms in the Guadiana River (southwestern Spain)

This paper describes the occurrence of toxic cyanobacteria along the Guadiana River over its course between Mérida and Badajoz (Extremadura, Spain). Water sampling for phytoplankton quantification and toxin analysis was carried out regularly between 1999 and 2001 in six different locations, including two shallow, slow-flowing river sites, two streamed river sites and two drinking water reservoirs. The cyanobacterial community differed significantly between these locations, especially during the summer. The predominant genera were Microcystis, Oscillatoria, Aphanizomenon and Anabaena. Using an ELISA assay the total microcystin contents of natural water samples from the most eutrophic locations ranged from 0.10 – 21.86 µg mcyst-LR equivalent·L-1 in Valdelacalzada and 0.10-11.3 µg mcyst-LR equivalent·L-1 in Vitonogales, and a seasonal variation of toxin content was observed. The amount of microcystins produced by each strain was determined by ELISA assay and the detection and identification of microcystin variants of three toxic strains of Microcystis aeruginosa was performed by high performance liquid chromatography (HPLC). The analysis of microcystins of the cultured strains revealed that toxin production was variable among different strains of M. aeruginosa isolated either from different blooms or from the same bloom.Junta de Andalucía (CTS358

Improving the use of natural and semi-natural grasslands in Mediterranean ruminant systems: issues, options and perspectives

Mediterranean small-ruminant systems (MSRS) have undergone an intensification process triggered by local and global changes. Recent research suggests that such tendency should be reversed. This paper provides insight about how increasing the contribution of grasslands (including natural and semi-natural, improved or temporary grasslands) to the feeding system can improve MSRS’ sustainability. Products from grazing animals are ethically appreciated and display good nutritional quality, specific organoleptic characteristics and possibly a longer shelf-life; they can be traced, and thus differentiated. The large surface area of natural and semi-natural grasslands (including areas with shrubs and trees) gives MSRS a head start in terms of carbon sequestration, which can be increased further by an appropriate grazing and feeding management. Technical options to increase grazed grass in MSRS include: (1) producing locally-adapted seed mixtures for grasslands; (2) increasing the proportion of legumes in grasslands; (3) managing grazing in order to maximize the amount and quality of intake; (4) matching the diversity of natural and semi-natural grasslands to animal requirements and/or providing strategic supplementation; (5) reconsidering the value of trees and the interest of foliage as forage. Digital technologies can help to improve farmers’ working conditions and build the ecological knowledge necessary to implement adaptive management strategies. At a socio-political level, it is important to recognize the peculiarities of silvopastoral systems, value the ecosystem services provided, foster collective management solutions and facilitate interconnection with other activities.ForageGrasslandsFeeding systemManagementAnimal performanceProduct qualityEcosystem servicePublishe

EMDR in pediatric hospital setting: a case report of an adolescent with cancer

IntroductionChildhood cancer is rare, but it is the most frequent serious event with a high risk of traumatization for children, adolescents, parents and siblings. EMDR is widely studied as clinical intervention that addressed cancer-related stressors among cancer adult population, but to our knowledge, no researches have been conducted among children and adolescent with cancer.MethodsThe purpose of this case study is to describe for the first time the application of the EMDR protocol in a pediatric hospital setting with a 17-years-old Italian adolescent who received a diagnosis of leukemia. He accessed the psychological support service complaining of feelings of anxiety and general discomfort. EMDR protocol started after the diagnosis and ended after the usual eight phases. The Impact of Event Scale—Revised (IES-R) was used to assess stress disorders symptoms as outcome at the baseline (before the First Phase) and at the end of the EMDR protocol (after the Eight Phase).ResultsBy using EMDR protocol the patient reported a decrease of emotional activation after a few EMDR sessions.ConclusionEMDR protocol may be effective for pediatric cancer patients in treating stress disorders symptoms and it can be proposed immediately after diagnosis as a standard care also in pediatric hospital setting

MLL5 improves ATRA driven differentiation and promotes xenotransplant engraftment in acute promyelocytic leukemia model

Although the mixed lineage leukemia 5 (MLL5) gene has prognostic implications in acute promyelocyte leukemia (APL), the underlying mechanism remains to be elucidated. Here, we demonstrate the critical role exerted by MLL5 in APL regarding cell proliferation and resistance to drug-induced apoptosis, through mtROS regulation. Additionally, MLL5 overexpression increased the responsiveness of APL leukemic cells to all-trans retinoic acid (ATRA)-induced differentiation, via regulation of the epigenetic modifiers SETD7 and LSD1. In silico analysis indicated that APL blasts with MLL5(high) transcript levels were associated with retinoic acid binding and downstream signaling, while MLL5(low) blasts displayed decreased expression of epigenetic modifiers (such as KMT2C, PHF8 and ARID4A). Finally, APL xenograft transplants demonstrated improved engraftment of MLL5-expressing cells and increased myeloid differentiation over time. Concordantly, evaluation of engrafted blasts revealed increased responsiveness of MLL5-expressing cells to ATRA-induced granulocytic differentiation. Together, we describe the epigenetic changes triggered by the interaction of MLL5 and ATRA resulting in enhanced granulocytic differentiation

que apoio ao diagnóstico de Borreliose de Lyme?

No diagnóstico de borreliose de Lyme, infecção sub-diagnosticada em Portugal, o apoio laboratorial é crucial para o tratamento atempado dos doentes, sob risco destes evoluírem para fases crónicas, resistentes à terapêutica. A difícil confirmação do agente etiológico através da cultura, aliada ao conhecimento actual sobre as elevadas taxas de falsos negativos nos testes serológicos de rastreio, bem como de casos seronegativos de doença, torna da maior importância a implementação da nova tecnologia de amplificação (PCR) de DNA borreliano no diagnóstico de rotina desta patologia. Tratando-se de uma técnica mais rápida e sensível tem-se revelado de maior sucesso do que a cultura. O presente trabalho compara a sensibilidade destas duas técnicas de detecção directa dos agentes do complexo Borrelia burgdorferi sensu lato, em 96 doentes com suspeita clínica desta afecção e em 569 vectores (ixodídeos) capturados em território nacional. Obteve-se o isolamento de borrélias em 1,3% das biópsias cutâneas e em 1,2% dos ixodídeos (carraças) não se tendo registado isolados noutros materiais biológicos humanos. A aplicação da técnica de nested PCR confirmou a presença de DNA de B. burgdorferi sensu lato em 24,8% das amostras analisadas. A identificação dos agentes patogénicos, por técnicas de hibridação (Reverse Line Blot), sequenciação e/ou RFLP, revelou a presença de quatro espécies genómicas: B. garinii, B. lusitaniae, B. valaisiana e B. afzelii. Em conclusão, a amplificação de DNA borreliano permite uma resposta mais rápida e sensível do que a cultura, contribuindo para um diagnóstico laboratorial mais eficaz da borreliose de Lyme humana. Lyme borreliosis (LB) is an under-diagnosed zoonosis in Portugal, specially due to the absence of specific clinical signs. The role of the laboratory diagnosis, together with an epidemiological information, is extremely important for the correct treatment of patients with this pathology. The sensitivity of two laboratory techniques (culture and DNA amplification by nested PCR) for direct detection of Borrelia burgdorferi sensu lato complex agents was evaluated in samples from 96 clinically suspected patients, as also in 569 vector ticks collected throughout Portugal. Borrelia genospecies were identified in 1.3% of the skin biopsies and in 1.2% of the vectors, after growth in selective culture medium (BSK). No growth was obtained from other type of samples. B.burgdorferi sensu lato DNA was present in 24.8% of analyzed samples, as per intergenic rRNA 5S-23S (rrf–rrl) spacer amplification by nested PCR. Four genomic species (B.garinii, B.lusitaniae, B.valaisiana and B.afzelii) were identified by DNA hybridization (Reverse-Line-Blot), sequencing and/or RFLP. In conclusion and taking into account the results found in this study, it seems that Borrelia DNA amplification technique is quicker and present higher sensitivity than culture, providing a more effective laboratory diagnosis of LB in human populations and in vectors.publishe

Sobrevivencia de corderos con servicio diferido : Incremento de la sobrevivencia perinatal en pariciones tardías

Un servicio diferido dentro de la estación reproductiva permite que la mayor oferta forrajera coincida con la preñez avanzada y la lactancia, y asegura la parición de corderos en una época climáticamente más favorable. Las experiencias con servicios diferidos de julio registran una elevada sobrevivencia perinatal, evidenciando la importancia de evaluar la implementación de esta práctica en los establecimientos ganaderos.Estación Experimental Agropecuaria BarilocheFil: Cueto, Marcela Isabel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; ArgentinaFil: Bidinost, Franca. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural Bariloche; ArgentinaFil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal. Grupo de Reproducción y Genética; ArgentinaFil: Villagra, Edgar Sebastian. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; ArgentinaFil: Villagra, Edgar Sebastian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentin

The impact of human breast milk components on the infant metabolism

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