Genetic differentiation in Bonga shad along West African shoreline recovered with 7 EPIC loci.

<p>Admixture proportions at each sampling location (black dots) of the 4 genetic clusters recovered by TESS are shown in pies-charts. Gene flow barriers are highlighted using the spatial autocorrelation method, Barrier 2.2. are indicated by violet bars on the map, and their reliability was estimated using a bootstrap procedure on seven <i>F</i>_ST matrices (one per nuclear locus). Line thickness of gene flow barriers is proportional to the bootstrap value and green numbers indicate the number of loci that report significant genetic heterogeneity (i.e. number of loci supporting a genetic break).</p

Sampling locations for Bonga shad.

<p>MAU: Etoile Bay, Mauritania; SEN: St Louis, Senegal; SAL : Foundiougne, Saloum, Senegal ; GAM : Tendaba, The Gambia ; CAS : Diogue, Casamance, Senegal ; GUI : Conakry, Guinea ; ICA : Aby, Côte d’Ivoire; CON: Loango Bay, Congo; ANG: Luanda, Angola. Grey areas represent estuarine conditions according to Charles-Dominique and Albaret [34]. Red dots indicate scientific sampling stations where Bonga shad were identified.</p

Smelt AFLP data - Dodson et al. 2015 (PlosONE)

This file contains AFLP genotypes (29 loci) for 447 Rainbow smelt (Osmerus mordax). One sheet contains the data, one sheet establishes the correspondence between Site numbers in the publication (Table 2 and Figure 2) and codes used for indiividual genotypes

DodsonAl_PlosONE15_MorphoData

This files contains morphological data. One sheet contains the data itself, one sheet establishes the correspondence between sampling sites in Figure 1 and Table 1 with the codes used in the datasheet; one sheet details the meaning of trait variables. These traits are also presented in the DodsonAl_PlosONE15_DescriptionVariables.pdf fil

Highly-correlated morphological traits, composing the two main axes of variation of a DFA differentiating rainbow smelt grouped according to glacial race and genetic admixture.

<p>Significant loadings were greater than +0.152 or lower than -0.152 at the 1% level (Bonferroni correction, alpha = 0.01/(2 x 42) = 0.000119); Fisher’s inverse hyperbolic tangent transformation. Traits with a negative loading on DFA axis 1 have higher trait values for mixed- Atlantic populations (located at the negative end of axis 1) and lower trait values for all other groups (located at the positive end of axis 1). Conversely, a positive loading on this axis had a lower trait value for mixed-Atlantic populations. Traits with a positive loading on axis 2 have higher trait values for mixed-Acadian populations and lower trait values for all other groups. Conversely, a negative loading on this axis had a lower trait value for mixed- Acadian populations. l.- length, d.- depth, ct.- count. Traits in bold print signify transgressive traits (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123172#pone.0123172.s003" target="_blank">S1 Table</a> for values).</p><p>Highly-correlated morphological traits, composing the two main axes of variation of a DFA differentiating rainbow smelt grouped according to glacial race and genetic admixture.</p

Map illustrating the location of sites in North-east North America sampled to describe the morphological diversity and mtDNA lineage composition of rainbow smelt populations.

<p>Pies represent the relative proportions of the two glacial races in each sample (see table <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123172#pone.0123172.t001" target="_blank">1</a> for place names, sample sizes and exact proportions). The black sectors represent the proportional representation of the mtDNA Acadian race, the open sectors, the mtDNA Atlantic race.</p

Sample mean and S.E.M of (a) Hybrid index (<i>h</i>) and Bayesian admixture proportions (<i>q</i>), with (b) and without (c) Loc Prior.

<p>The 34 samples are arranged as per <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123172#pone.0123172.g003" target="_blank">Fig 3</a> in downstream order with spawning sites (1–16) followed by winter fishery samples (18–29) and the YOY samples (30–34).</p

Estimated population structure as obtained in structure with Loc Prior.

<p>Each individual is represented by a thin vertical line, which is partitioned into <i>K</i> colored segments that represent the individual's estimated ancestry within each <i>K</i> cluster. Two values of <i>K</i> are presented; upper panel: <i>K</i> = 2 (optimal <i>K</i> using Evanno’s criterion) and lower panel: <i>K</i> = 7 (optimal K using Pritchard’s criterion. Black lines separate individuals of different sampling sites. Population clusters are labeled above the Fig., individual sampling sites and sample category, below the Fig.</p

Region, sample IDs, sample site locations, and sample sizes for morphological and mtDNA analyses of rainbow smelt.

<p>All fish were adults and were sampled between February and September, 2002. Percent composition of mtDNA Acadian lineage from Barrette [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123172#pone.0123172.ref029" target="_blank">29</a>].</p><p>SLR- St. Lawrence River.</p

Discriminant function analysis significantly differentiating smelt from populations characterized by the absence or presence of genetic admixture of the two glacial races.

<p>Canonical axes have a relative discriminative power of 58.66% (axis 1; <i>P</i>< 0.0001) and 26.64% (axis 2; <i>P</i>< 0.0001); N = 576. For morphological traits that contribute significantly to the formation of each canonical axis, see table <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123172#pone.0123172.t004" target="_blank">4</a>. Each point represents the mean group value. Horizontal and vertical bars show the 95% confidence intervals around the mean value. Open symbols denote groups from the Acadian race (A) and filled symbols denote groups from the Atlantic race (B). Triangles denote fish from populations with no evidence of genetic admixture of the two races (A-pure, B-pure) and circles denote groups of hybridized fish of predominantly Acadian (A-Mixed) or Atlantic (B-Mixed) origin.</p