THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS

Objective: This study aims to evaluate the relation between hypercholesterolemia and aging, beside the role of Ulva fasciata polysaccharides (UFP)aqueous extracts in lowering cholesterol in aged hypercholesterolemia-induced rats was demonstrated.Method: A total of 140 male Wister rats weighing 120±10 g, 6-9 months old were used. Hypercholesterolemia was induced in rats by feeding ratshigh-fat diet (cholesterol), cholesterol was orally administrated at a dose of (30 mg/0.3 ml olive oil/1 kg animal) 5 times a week for 12 consecutiveweeks, lard fat was mixed with normal diet (1 kg of animal lard was added to 5 kg of normal diet), the occurrence of hypercholesterolemia wasdetermined by measuring the lipid profile (TC, LDL-C, high-density lipoprotein-cholesterol [HDL-C], triglyceride [TG]), the old hypercholesterolemic(HC) rats were only used.Results: The antihypercholesterolemic (HC) effects of ulvan, the sulfated polysaccharide extracted from the green alga Ulvafasciata, in aged rats,were studied. Algal treatment declared a significant reduction in serum total lipid level while, elevation of high-density lipoprotein-cholesterol levelwas noticed in HC rats. Moreover, the algal treatment significantly decreased serum liver and kidney functions biomarkers and improved the hepaticantioxidant levels in hyperlipidemic aged rats. In addition, ulvan administration significantly suppressed the expression of tumor necrosis factoralpha,myeloperoxidaseand cell vascularand intracellularadhesionmolecules-1,whileincreasedthe anti-inflammatorycytokinelevel;interleukin-10.Furthermore,the histopathologicalexaminationof aorta,liverandkidneyof HC-treatedratsindicatedthatthe Ulvafasciatapolysaccharides(UFP),isapotentnaturalhypolipidemicnutraceuticalforthe ameliorationof hyperlipidemiain aged rats.Conclusion: It could be concluded that, in comparison with the standard anti-HC drug (fluvastatin) used in this study, both cold and hot UFP algalextracts of U. fasciata demonstrated appreciable anti-hypercholesterolemic property, in addition to their antioxidant activity even in the old HC stressed rats. Thus, it could be used as a natural lipid regulator.Keywords: Ulvafasciata, Hypercholesterolemia, Polysaccharides, Rats, Sulfated polysaccharides, Aging, Hyper-cholesterolemia

SULPHATED POLYSACCHARIDES (SPS) FROM THE GREEN ALGA ULVA FASCIATA EXTRACT MODULATES LIVER AND KIDNEY FUNCTION IN HIGH FAT DIET-INDUCED HYPERCHOLESTEROLEMIC RATS

Objective: Hypercholesterolemia (HC) was frequently associated with oxidative stress, and release of inflammatory cytokines is to determine the hypolipidemic effects of sulphated polysaccharides from seaweed Ulva fasciata algal extracts through measuring the activities of some parameters related to liver and kidney functions in the serum of hypercholesterolemic rats as compared to normal one.Methods: Different groups of rats were administered a high cholesterol diet. Liver and kidney functions, inflammatory cytokines (TNF-α, CRP, MPO and IL-10), oxidative stress (GSH, MDA and NO), in addition to cell adhesion molecules (ICAM-1 and VCAM-1) were assessed before and after treatment with the algal polysaccharides. In addition, histological examination of liver and kidney were performed to confirm the biochemical findings.Results: The obtained results showed that oxidative stress and inflammatory markers associated with hypercholesterolemia were significantly increased in HC-rats. The histopathological examination of liver and kidney demonstrated severe degeneration with diffuse vacuolar degeneration, necrosis and the presence of fatty droplets. In addition; nephron-histological examination revealed, mild glomerular injury with mild vascular and inflammatory changes. Treatment with the algal sulphated polysaccharides effectively improved these disorders and diminished the formation of fatty liver, as well as renal dysfunction more than the reference drug; fluvastatin. Conclusion: It could be concluded that the consumption of UFP (Ulva fasciata polysaccharides), may be associated with attenuation of inflammatory markers, amelioration of fatty liver and improvement of renal dysfunction, that in turn lead to counteract hypercholesterolemia and its related disorders; such as obesity, and heart disease.Keywords: Non-alcoholic fatty liver disease, Seaweed, Ulva fasciata, Hypercholesterolemia, Hypolipidemic activity, Sulphated polysaccharides (SPs