Impact of field headlands on wheat and barley performance in a cool Atlantic climate as assessed in 40 Irish tillage fields

peer reviewedThe reduction in cereal crop yields on field headlands has previously been examined in other geographical regions, with research focusing on the relationship between yield and the distance from the crop edge. Headlands are subject to greater machinery trafficking than the centre of the field and the level of traffic imposed depends on the machine size and adopted turning practice. The aim of this work was to examine the impact of turning headlands on crop performance in a survey of 40 field sites in Irish conditions. The headland was categorised into three distinct zones: the area next to the field edge subject to moderate traffic intensities (field edge); the main headland area of greatest turning traffic (turning) and a transition zone (transition). An in-field zone (in-field) in which no machinery turns occur was also included. The 2-year survey included sites from three regions, four soil texture classes and had crops established with plough-based systems. Crop measurements, including plant densities, shoot counts and light interception, and yields were recorded at each site and included winter barley (WB), spring barley (SB) and winter wheat (WW) crops. The yield response of each crop type varied with sample zone, region and soil texture. There were significant (P < 0.001) yield differences recorded between the turning area and in-field zone for all three crops. Winter barley yields were reduced by 1.3 t/ha in the turning zone compared with the in-field section, while SB and WW had yield reductions of 2.08 and 4.04 t/ha, respectively, between these two field zones

Improved Technologies For Baled Silage.

End of Project ReportThis present report summaries the findings of the more recent research on baled silage.The contents of this report are presented under the following headings:1. Characteristics of baled silage on Irish farms - a survey 2. Mechanisation in the field 3. Forage and plastic 4. Schzophyllum commune 5. Preventing wildlife damage 6. Growth by cattl

HIF1α drives chemokine factor pro-tumoral signaling pathways in acute myeloid leukemia

Approximately 80% of patients diagnosed with acute myeloid leukemia (AML) die as a consequence of failure to eradicate the tumor from the bone marrow microenvironment. We have recently shown that stroma-derived interleukin-8 (IL-8) promotes AML growth and survival in the bone marrow in response to AML-derived macrophage migration inhibitory factor (MIF). In the present study we show that high constitutive expression of MIF in AML blasts in the bone marrow is hypoxia-driven and, through knockdown of MIF, HIF1α and HIF2α, establish that hypoxia supports AML tumor proliferation through HIF1α signaling. In vivo targeting of leukemic cell HIF1α inhibits AML proliferation in the tumor microenvironment through transcriptional regulation of MIF, but inhibition of HIF2α had no measurable effect on AML blast survival. Functionally, targeted inhibition of MIF in vivo improves survival in models of AML. Here we present a mechanism linking HIF1α to a pro-tumoral chemokine factor signaling pathway and in doing so, we establish a potential strategy to target AML

Prostate Cancer Cell Lines under Hypoxia Exhibit Greater Stem-Like Properties

Hypoxia is an important environmental change in many cancers. Hypoxic niches can be occupied by cancer stem/progenitor-like cells that are associated with tumor progression and resistance to radiotherapy and chemotherapy. However, it has not yet been fully elucidated how hypoxia influences the stem-like properties of prostate cancer cells. In this report, we investigated the effects of hypoxia on human prostate cancer cell lines, PC-3 and DU145. In comparison to normoxia (20% O2), 7% O2 induced higher expressions of HIF-1α and HIF-2α, which were associated with upregulation of Oct3/4 and Nanog; 1% O2 induced even greater levels of these factors. The upregulated NANOG mRNA expression in hypoxia was confirmed to be predominantly retrogene NANOGP8. Similar growth rates were observed for cells cultivated under hypoxic and normoxic conditions for 48 hours; however, the colony formation assay revealed that 48 hours of hypoxic pretreatment resulted in the formation of more colonies. Treatment with 1% O2 also extended the G0/G1 stage, resulting in more side population cells, and induced CD44 and ABCG2 expressions. Hypoxia also increased the number of cells positive for ABCG2 expression, which were predominantly found to be CD44bright cells. Correspondingly, the sorted CD44bright cells expressed higher levels of ABCG2, Oct3/4, and Nanog than CD44dim cells, and hypoxic pretreatment significantly increased the expressions of these factors. CD44bright cells under normoxia formed significantly more colonies and spheres compared with the CD44dim cells, and hypoxic pretreatment even increased this effect. Our data indicate that prostate cancer cells under hypoxia possess greater stem-like properties

Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation

Cord blood (CB) has been used as a viable source of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) in over 35,000 clinical hematopoietic cell transplantation (HCT) efforts to treat the same variety of malignant and non-malignant disorders treated by bone marrow (BM) and mobilized peripheral blood (mPB) using HLA-matched or partially HLA-disparate related or unrelated donor cells for adult and children recipients. This review documents the beginning of this clinical effort that started in the 1980's, the pros and cons of CB HCT compared to BM and mPB HCT, and recent experimental and clinical efforts to enhance the efficacy of CB HCT. These efforts include means for increasing HSC numbers in single CB collections, expanding functional HSCs ex vivo, and improving CB HSC homing and engraftment, all with the goal of clinical translation. Concluding remarks highlight the need for phase I/II clinical trials to test the experimental procedures that are described, either alone or in combination

An inherited defect in the C3 convertase, C3b,Bb, associated with glomerulonephritis

An inherited defect in the C3 convertase, C3b,Bb, associated with glomerulonephritis. The control of the amplification C3 convertase, C3b,Bb, of the serum complement system has been found to be defective in five members of a family spanning three generations. One of the five has membranoproliferative glomerulonephritis (MPGN) type III and another has mild idiopathic rapidly progressive glomerulonephritis. The defect is manifested by low serum concentrations of C3 and usually factor B with normal levels of the proteins which control the convertase, H and I. C3 nephritic factor (C3NeF) was not demonstrable. Enhanced C3 conversion was produced by the incubation of their serum at 37°C for 30min. This conversion was further accelerated by incubation after increasing the serum magnesium concentration by increments ranging from 0.25 to 1.9mM. Incremental additions of H to serum depleted of H indicated that the amplification convertase of affected family members required more H for its inhibition than did that of normal subjects. This requirement was reduced by the addition of purified normal C3 but not by the addition of purified C3 of the propositus. It is postulated that affected family members are heterozygous for a gene producing an abnormal C3 which, as a constituent of the amplification convertase, C3b,Bb, confers resistance to H. Investigation of this apparently nephritogenic defect may provide insight into the pathogenesis of these glomerulonephritides.Déficit héréditaire de la C3 convertase, C3b,Bb, associé à une glomérulonéphrite. Le contrôle de la convertase d'amplification de C3, C3b,Bb, du complément sérique a été trouvé défectueux chez cinq membres d'une famille couvrant trois générations. Un des cinq avait une glomérulonéphrite membrano-proliférative (MPGN) de type III et un autre avait une glomérulonéphrite idiopathique rapidement progressive. Le défaut se manifeste par des concentrations faibles de C3 et habituellement de facteur B sériques avec des niveaux normaux des protéines contrôlant la convertase H et I. Le facteur néphritique C3 (C3NeF) n'était pas démontrable. Une augmentation de la C3 conversion a été produite par l'incubation de leur sérum à 37°C pendant 30min. Cette conversion était encore plus accélérée par incubation avec augmentation de la concentration de magnésium sérique dans des valeurs comprises entre 0,25 et 1,9mM. Des augmentations progressives d'H au sérum dépiété en H ont montré que la convertase d'amplification des membres de la famille touchée nécessitait plus d'H pour son inhibition que cela était nécessaire aux sujets normaux. Ce besoin était réduit en ajoutant du C3 normal purifié, mais non du C3 purifié des propositus. Il est postulé que les membres des familles affectées sont hétérozygotes pour un gène produisant un C3 anormal, lequel, étant un constituant de la convertase d'amplification C3b,Bb, confère une résistance à H. L'investigation de ce défaut en apparence néphritogène pourrait fournir des informations sur la pathogénie de ces glomérulonéphrites

First Detection and Characterization of Cross- and Multiple Resistance to Acetyl-CoA Carboxylase (ACCase)- and Acetolactate Synthase (ALS)-Inhibiting Herbicides in Black-Grass (Alopecurus myosuroides) and Italian Ryegrass (Lolium multiflorum) Populations from Ireland

Understanding the resistance spectrum and underlying genetic mechanisms is critical for managing herbicide-resistant populations. In this study, resistance to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors was investigated in four suspected resistant populations of Alopecurus myosuroides (ALOMY-001 to ALOMY-004) and Lolium multiflorum (LOLMU-001 to LOLMU-004), collected from cereal production fields in Ireland. Glasshouse assays with three ALOMY-active herbicides [propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron (ALS)] or five LOLMU-active herbicides [pinoxaden, propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron, pyroxsulam (ALS)], and target-site resistance mechanism studies, based on pyrosequencing, were carried out in each of those populations. For A. myosuroides, Ile-1781-Leu ACCase mutation contributed to propaquizafop and cycloxydim resistance (shoot dry weight GR50 resistance factor (RF) = 7.5&ndash;35.5) in all ALOMY populations, and the independent Pro-197-Thr or Pro-197-Ser ALS mutation contributed to mesosulfuron + iodosulfuron resistance (RF = 3.6&ndash;6.6), in ALOMY-002 to ALOMY-004. Most of the analyzed plants for these mutations were homo/heterozygous combinations or only heterozygous. For L. multiflorum, phenotypic resistance to mesosulfuron + iodosulfuron (RF = 11.9&ndash;14.6) and pyroxsulam (RF = 2.3&ndash;3.1) was noted in all LOLMU populations, but the Pro-197-Gln or Pro-197-Leu ALS mutation (mostly in homozygous status) was identified in LOLMU-001, LOLMU-002 and LOLMU-004 only. Additionally, despite no known ACCase mutations in any LOLMU populations, LOLMU-002 survived pinoxaden and propaquizafop application (RF = 3.4 or 1.3), and LOLMU-003 survived pinoxaden (RF = 2.3), suggesting the possibility of non-target-site resistance mechanisms for ACCase and/or ALS resistance in these populations. Different resistance levels, as evidenced by a reduction in growth as dose increased above field rates in ALOMY and LOLMU, were due to variations in mutation rate and the level of heterozygosity, resulting in an overall resistance rating of low to moderate. This is the first study confirming cross- and multiple resistance to ACCase- and ALS-inhibiting herbicides, highlighting that resistance monitoring in A. myosuroides and L. multiflorum in Ireland is critical, and the adoption of integrated weed management strategies (chemical and non-chemical/cultural strategies) is essential

Improved Technologies For Baled Silage.

End of Project ReportThis present report summaries the findings of the more recent research on baled silage.The contents of this report are presented under the following headings:1. Characteristics of baled silage on Irish farms - a survey 2. Mechanisation in the field 3. Forage and plastic 4. Schzophyllum commune 5. Preventing wildlife damage 6. Growth by cattl