Antioxidants in Animal Nutrition: UHPLC-ESI-QqTOF Analysis and Effects on In Vitro Rumen Fermentation of Oak Leaf Extracts

: The genus Quercus supplies a large amount of residual material (e.g., bark, acorns, leaves, wood), the valorization of which can favor a supply of antioxidant polyphenols to be used in the pharmaceutical, nutraceutical, or cosmeceutical sector. The recovery of specialized metabolites could also benefit livestock feeding, so much so that polyphenols have gained attention as rumen fermentation modifiers and for mitigating the oxidative imbalance to which farm animals are subject. In this context, leaves of Quercus robur L. from Northern Germany were of interest and the alcoholic extract obtained underwent an untargeted profiling by means of ultra-high-performance liquid chromatography/high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) techniques. As triterpenes and fatty acids occurred, the alcoholic extract fractionation pointed out the obtainment of a polyphenol fraction, broadly constituted by coumaroyl flavonol glycosides and condensed tannins. Total phenol, flavonoid and condensed tannins content assays, as well as antiradical (DPPH● and ABTS+●) and reducing activity (PFRAP) were carried out on the alcoholic extract and its fractions. When the effects on rumen liquor was evaluated in vitro in terms of changes in fermentation characteristics, it was observed that oak leaf extract and its fractions promoted an increase in total volatile fatty acids and differently modulated the relative content of each fatty acid

Castanea sativa Mill. Leaf: UHPLC-HR MS/MS Analysis and Effects on In Vitro Rumen Fermentation and Methanogenesis

: Castanea sativa Mill. (Fagaceae) is a deciduous tree grown for its wood and edible fruits. Chestnut processing produces residues (burs, shells, and leaves) exploitable for their diversity in bioactive compounds in animal nutrition. In fact, plant-specialized metabolites likely act as rumen modifiers. Thus, the recovery of residual plant parts as feed ingredients is an evaluable strategy. In this context, European chestnut leaves from northern Germany have been investigated, proving to be a good source of flavonoids as well as gallo- and ellagitannins. To this purpose, an alcoholic extract was obtained and an untargeted profiling carried out, mainly by means of ultra-high-performance liquid chromatography/high-resolution tandem mass spectrometry (UHPLC-HR MS/MS) techniques. To better unravel the polyphenol constituents, fractionation strategies were employed to obtain a lipophilic fraction and a polar one. This latter was highly responsive to total phenolic and flavonoid content analyses, as well as to antiradical (DPPH● and ABTS+●) and reducing activity (PFRAP) assays. The effect of the alcoholic extract and its fractions on rumen liquor was also evaluated in vitro in terms of fermentative parameter changes and impact on methanogenesis. The data acquired confirm that chestnut leaf extract and the fractions therefrom promote an increase in total volatile fatty acids, while decreasing acetate/propionate ratio and CH4 production

Penile cancer: prognostic and predictive factors in clinical decision-making

: Penile cancer (PC) is a typical tumor of non-industrialized countries. The incidence is 20-30 times higher in Africa and South America, considering the elevated prevalence of sexually transmitted diseases. Histologically, PC includes squamous cell carcinoma (SCPC), the most frequent, and nonsquamous carcinoma (NSCPC). Early diagnosis is the goal, whereas later diagnosis relates to poor functional outcomes and worse prognosis. The 5-year survival rate is 85% for patients with histologically regional negative lymph nodes, compared to 29%-40% for those with histologically regional positive lymph nodes. To date no new drugs are approved, and there are few new data about molecular mechanisms underlying tumorigenesis. The SCPC remains a rare tumor and the current therapeutic algorithm is based principally on retrospective analysis and less on prospective trials. In this review article, biomarkers of prognosis and efficacy of current treatments are summarized with a focus on those that have the potential to affect treatment decision-making in SCPC

Measurement of boron and carbon fluxes in cosmic rays with the PAMELA experiment

The propagation of cosmic rays inside our galaxy plays a fundamental role in shaping their injection spectra into those observed at Earth. One of the best tools to investigate this issue is the ratio of fluxes for secondary and primary species. The boron-to-carbon (B/C) ratio, in particular, is a sensitive probe to investigate propagation mechanisms. This paper presents new measurements of the absolute fluxes of boron and carbon nuclei, as well as the B/C ratio, from the PAMELA space experiment. The results span the range 0.44 - 129 GeV/n in kinetic energy for data taken in the period July 2006 - March 2008

The cosmic-ray positron energy spectrum measured by PAMELA

Precision measurements of the positron component in the cosmic radiation provide important information about the propagation of cosmic rays and the nature of particle sources in our Galaxy. The satellite-borne experiment PAMELA has been used to make a new measurement of the cosmic-ray positron flux and fraction that extends previously published measurements up to 300 GeV in kinetic energy. The combined measurements of the cosmic-ray positron energy spectrum and fraction provide a unique tool to constrain interpretation models. During the recent solar minimum activity period from July 2006 to December 2009 approximately 24500 positrons were observed. The results cannot be easily reconciled with purely secondary production and additional sources of either astrophysical or exotic origin may be required.Comment: 14 pages, 4 figures, 1 table. Accepted for publication in Physical Review Letters. Corrected a typo in the flux units of Table

PAMELA's measurements of geomagnetic cutoff variations during solar energetic particle events

Data from the PAMELA satellite experiment were used to measure the geomagnetic cutoff for high-energy ($\gtrsim$ 80 MeV) protons during the solar particle events on 2006 December 13 and 14. The variations of the cutoff latitude as a function of rigidity were studied on relatively short timescales, corresponding to single spacecraft orbits (about 94 minutes). Estimated cutoff values were cross-checked with those obtained by means of a trajectory tracing approach based on dynamical empirical modeling of the Earth's magnetosphere. We find significant variations in the cutoff latitude, with a maximum suppression of about 6 deg for $\sim$80 MeV protons during the main phase of the storm. The observed reduction in the geomagnetic shielding and its temporal evolution were compared with the changes in the magnetosphere configuration, investigating the role of IMF, solar wind and geomagnetic (Kp, Dst and Sym-H indexes) variables and their correlation with PAMELA cutoff results.Comment: Conference: The 34th International Cosmic Ray Conference (ICRC2015), 30 July - 6 August, 2015, The Hague, The Netherlands, Volume: PoS(ICRC2015)28

Time dependence of the e^- flux measured by PAMELA during the July 2006 - December 2009 solar minimum

Precision measurements of the electron component in the cosmic radiation provide important information about the origin and propagation of cosmic rays in the Galaxy not accessible from the study of the cosmic-ray nuclear components due to their differing diffusion and energy-loss processes. However, when measured near Earth, the effects of propagation and modulation of galactic cosmic rays in the heliosphere, particularly significant for energies up to at least 30 GeV, must be properly taken into account. In this paper the electron (e^-) spectra measured by PAMELA down to 70 MeV from July 2006 to December 2009 over six-months time intervals are presented. Fluxes are compared with a state-of-the-art three-dimensional model of solar modulation that reproduces the observations remarkably well.Comment: 40 pages, 18 figures, 1 tabl

Search for anisotropies in cosmic-ray positrons detected by the PAMELA experiment

The PAMELA detector was launched on board of the Russian Resurs-DK1 satellite on June 15, 2006. Data collected during the first four years have been used to search for large-scale anisotropies in the arrival directions of cosmic-ray positrons. The PAMELA experiment allows for a full sky investigation, with sensitivity to global anisotropies in any angular window of the celestial sphere. Data samples of positrons in the rigidity range 10 GV $\leq$ R $\leq$ 200 GV were analyzed. This article discusses the method and the results of the search for possible local sources through analysis of anisotropy in positron data compared to the proton background. The resulting distributions of arrival directions are found to be isotropic. Starting from the angular power spectrum, a dipole anisotropy upper limit \delta = 0.166 at 95% C.L. is determined. Additional search is carried out around the Sun. No evidence of an excess correlated with that direction was found.Comment: The value of the dipole anisotropy upper limit has been changed. The method is correct but there was a miscalculation in the relative formul

Solar energetic particle events: trajectory analysis and flux reconstruction with PAMELA

The PAMELA satellite experiment is providing first direct measurements of Solar Energetic Particles (SEPs) with energies from about 80 MeV to several GeV in near-Earth space, bridging the low energy data by other space-based instruments and the Ground Level Enhancement (GLE) data by the worldwide network of neutron monitors. Its unique observational capabilities include the possibility of measuring the flux angular distribution and thus investigating possible anisotropies. This work reports the analysis methods developed to estimate the SEP energy spectra as a function of the particle pitch-angle with respect to the Interplanetary Magnetic Field (IMF) direction. The crucial ingredient is provided by an accurate simulation of the asymptotic exposition of the PAMELA apparatus, based on a realistic reconstruction of particle trajectories in the Earth's magnetosphere. As case study, the results for the May 17, 2012 event are presented.Comment: Conference: The 34th International Cosmic Ray Conference (ICRC2015), 30 July - 6 August, 2015, The Hague, The Netherlands, Volume: PoS(ICRC2015)08