Display of a Maize cDNA library on baculovirus infected insect cells

<p>Abstract</p> <p>Background</p> <p>Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies for efficient proteomic analysis are needed. Baculovirus expression systems have been used for the last twenty years to express in insect cells a wide variety of eukaryotic proteins that require complex folding or extensive posttranslational modification. More recently, baculovirus display technologies based on the expression of foreign sequences on the surface of <it>Autographa californica </it>(AcMNPV) have been developed. We investigated the potential of a display methodology for a cDNA library of maize young seedlings.</p> <p>Results</p> <p>We constructed a full-length cDNA library of young maize etiolated seedlings in the transfer vector pAcTMVSVG. The library contained a total of 2.5 × 10⁵independent clones. Expression of two known maize proteins, calreticulin and auxin binding protein (ABP1), was shown by western blot analysis of protein extracts from insect cells infected with the cDNA library. Display of the two proteins in infected insect cells was shown by selective biopanning using magnetic cell sorting and demonstrated proof of concept that the baculovirus maize cDNA display library could be used to identify and isolate proteins.</p> <p>Conclusion</p> <p>The maize cDNA library constructed in this study relies on the novel technology of baculovirus display and is unique in currently published cDNA libraries.</p> <p>Produced to demonstrate proof of principle, it opens the way for the development of a eukaryotic <it>in vivo </it>display tool which would be ideally suited for rapid screening of the maize proteome for binding partners, such as proteins involved in hormone regulation or defence.</p

The influence of Survivor stories and a virtual reality representation of a residential school on reconciliation in Canada

Indigenous Peoples in Canada have endured many genocidal efforts, such as residential schools. Across the country, initiatives to promote critical historical education about residential schools are underway, ranging in duration, content, and immersion. In this study, we tested whether a promising high-immersion approach, a virtual reality residential school, could improve non-Indigenous participants' attitudes and feelings toward Indigenous people. We compared the effects of the virtual residential school to a transcript condition, in which participants read the transcripts of the narration that accompanied the virtual residential school, and an empty control condition. The study had three time points: Baseline (N = 241), intervention (N = 241), and follow-up (N = 132). Immediately following the intervention, what participants learned about the residential school, both through virtual reality and reading the transcripts, increased non-Indigenous participants' empathy, political solidarity, and outgroup warmth for Indigenous people, relative to the control. The virtual reality school, but not transcripts, also increased privity relative to the control. These effects decreased over time. In summary, though both written and virtual reality forms of critical historical education were effective in the short term, to maintain the long-term effects of critical historical education, ongoing or recurring education is likely necessary. These results extend the virtual reality literature to unstudied concepts (political solidarity, privity) and critical historical education literature to a new form of media (virtual reality). We discuss the findings in relation to literature on critical historical education and virtual reality as well as outline future directions

The stranding anomaly as population indicator: the case of Harbour Porpoise <i>Phocoena phocoena</i> in North-Western Europe

Ecological indicators for monitoring strategies are expected to combine three major characteristics: ecological significance, statistical credibility, and cost-effectiveness. Strategies based on stranding networks rank highly in cost-effectiveness, but their ecological significance and statistical credibility are disputed. Our present goal is to improve the value of stranding data as population indicator as part of monitoring strategies by constructing the spatial and temporal null hypothesis for strandings. The null hypothesis is defined as: small cetacean distribution and mortality are uniform in space and constant in time. We used a drift model to map stranding probabilities and predict stranding patterns of cetacean carcasses under H-0 across the North Sea, the Channel and the Bay of Biscay, for the period 1990-2009. As the most common cetacean occurring in this area, we chose the harbour porpoise <i>Phocoena phocoena</i> for our modelling. The difference between these strandings expected under H-0 and observed strandings is defined as the stranding anomaly. It constituted the stranding data series corrected for drift conditions. Seasonal decomposition of stranding anomaly suggested that drift conditions did not explain observed seasonal variations of porpoise strandings. Long-term stranding anomalies increased first in the southern North Sea, the Channel and Bay of Biscay coasts, and finally the eastern North Sea. The hypothesis of changes in porpoise distribution was consistent with local visual surveys, mostly SCANS surveys (1994 and 2005). This new indicator could be applied to cetacean populations across the world and more widely to marine megafauna

Testing Multiple Coordination Constraints with a Novel Bimanual Visuomotor Task

The acquisition of a new bimanual skill depends on several motor coordination constraints. To date, coordination constraints have often been tested relatively independently of one another, particularly with respect to isofrequency and multifrequency rhythms. Here, we used a new paradigm to test the interaction of multiple coordination constraints. Coordination constraints that were tested included temporal complexity, directionality, muscle grouping, and hand dominance. Twenty-two healthy young adults performed a bimanual dial rotation task that required left and right hand coordination to track a moving target on a computer monitor. Two groups were compared, either with or without four days of practice with augmented visual feedback. Four directional patterns were tested such that both hands moved either rightward (clockwise), leftward (counterclockwise), inward or outward relative to each other. Seven frequency ratios (3∶1, 2∶1, 3∶2, 1∶1, 2∶3. 1∶2, 1∶3) between the left and right hand were introduced. As expected, isofrequency patterns (1∶1) were performed more successfully than multifrequency patterns (non 1∶1). In addition, performance was more accurate when participants were required to move faster with the dominant right hand (1∶3, 1∶2 and 2∶3) than with the non-dominant left hand (3∶1, 2∶1, 3∶2). Interestingly, performance deteriorated as the relative angular velocity between the two hands increased, regardless of whether the required frequency ratio was an integer or non-integer. This contrasted with previous finger tapping research where the integer ratios generally led to less error than the non-integer ratios. We suggest that this is due to the different movement topologies that are required of each paradigm. Overall, we found that this visuomotor task was useful for testing the interaction of multiple coordination constraints as well as the release from these constraints with practice in the presence of augmented visual feedback

H.E.S.S. observations of PKS 2155-304

The high-frequency peaked BL Lac PKS 2155-304 at redshift z = 0.117 has been detected with high significance (∼45σ) at energies greater than 160 GeV, using the H.E.S.S. stereoscopic array of imaging air-Cherenkov telescopes in Namibia. A strong signal is found in each of the data sets corresponding to the dark periods of July and October, 2002, and June-September, 2003. The observed flux of VHE gamma rays shows variability on time scales of months, days, and hours. The monthly-averaged integral flux above 300 GeV varies between 10% and 60% of the flux observed from the Crab Nebula. Energy spectra are measured for these individual periods of data taking and are characterized by a steep power law with a time-averaged photon index of Γ = 3.32 ± 0.06. An improved x2 per degree of freedom is found when either a power law with an exponential cutoff energy or a broken power law are fit to the time-averaged energy spectrum. However, the significance of the improvement is marginal (∼2σ). The suggested presence of features in the energy spectrum may be intrinsic to the emission from the blazar, or an indication of absorption of TeV gamma rays by the extragalactic infrared background light.F. Aharonian, A. G. Akhperjanian, K.-M. Aye, A. R. Bazer-Bachi, M. Beilicke, W. Benbow, D. Berge, P. Berghaus, K. Bernlöhr, O. Bolz, C. Boisson, C. Borgmeier, F. Breitling, A. M. Brown, J. Bussons Gordo, P. M. Chadwick, V. R. Chitnis, L.-M. Chounet, R. Cornils, L. Costamante, B. Degrange, A. Djannati-Ataï, L.O'C. Drury, T. Ergin, P. Espigat, F. Feinstein, P. Fleury, G. Fontaine, S. Funk, Y. A. Gallant, B. Giebels, S. Gillessen, P. Goret, J. Guy, C. Hadjichristidis, M. Hauser, G. Heinzelmann, G. Henri, G. Hermann, J. A. Hinton, W. Hofmann, M. Holleran, D. Horns, O. C. de Jager, I. Jung, B. Khélifi, Nu. Komin, A. Konopelko, I. J. Latham, R. Le Gallou, M. Lemoine, A. Lemière, N. Leroy, T. Lohse, A. Marcowith, C. Masterson, T. J. L. McComb, M. de Naurois, S. J. Nolan, A. Noutsos, K. J. Orford, J. L. Osborne, M. Ouchrif, M. Panter, G. Pelletier, S. Pita, M. Pohl, G. Pühlhofer, M. Punch, B. C. Raubenheimer, M. Raue, J. Raux, S. M. Rayner, I. Redondo, A. Reimer, O. Reimer, J. Ripken, M. Rivoal, L. Rob, L. Rolland, G. Rowell, V. Sahakian, L. Saugé, S. Schlenker, R. Schlickeiser, C. Schuster, U. Schwanke, M. Siewert, H. Sol, R. Steenkamp, C. Stegmann, J.-P. Tavernet, C. G. Théoret, M. Tluczykont, D. J. van der Walt, G. Vasileiadis, P. Vincent, B. Visser, H. J. Völk and S. J. Wagne

Pensin monitoimipaarien ergonominen ja monipuolinen käyttö

Opinnäytetyön tarkoitus on, että Pensin monitoimipaarien käyttöön suunnitellaan opas. Toteutus on tehty yhteistyössä monitoimipaarien valmistajan Pensi Rescue Oy:n kanssa. Pensin monitoimipaarit ovat kotimaiset ja laajalti käytössä Suomessa ja muuallakin. Opinnäytetyön tuote on n. 30 sivuinen opas nimeltään Pensiapuopas. Se on tarkoitettu 9Lives Sastamalan yksikön ensihoidon opiskelijoille sekä sen uusille työntekijöille. Kokeneemmille ensihoitajille opas toimii muistutuksena ja antaa syventäviä tietoja Pensin paarien monipuolisesta käytöstä. Ensihoidon koulutuksessa ergonomiaa ja paarien turvallista käyttöä käsitellään vain vähän. Kuitenkin ensihoitotyössä paarityöskentely on keskeistä. Paarien oikea ja monipuolinen käyttö ansaitsee enemmän huomiota. Siksi paarien tarkoituksenmukainen käsittely työ- ja potilasturvallisuuden kannalta on otettu esille. Ensihoitotyö on fyysisesti kuormittavaa potilaan nostojen ja siirtojen vuoksi. Siirrot vaativat ripeyttä, varmuutta, tarkkuutta ja hyvää suunnittelua. Potilas voi olla vakavasti sairas tai loukkaantunut eikä häneltä voi vaatia siirtotilanteissa omaa suorituskykyä. Lisäksi haasteena on vaihteleva ja usein ahdas toimintaympäristö. Tästä johtuen siirtovälineen hallinta on keskeistä ensihoidon koulutuksessa ja työtehtävien turvallisessa suorittamisessa. Pensin paarien monipuolisesta käytöstä on Pensi Rescue Oy julkaissut opastusvideon. Pensiapuopas täydentää valmistajan tekemää videota ja palvelee muun muassa 9Lives Sastamalan työntekijöiden käyttökoulutustarpeita.This Bachelor`s thesis is a functional thesis. The purpose of this study was to produce a practical instructions manual called "Pensiapuopas" for the paramedic students and new employees working in the 9Lives organization in Sastamala. The handbook helps as well as the permanent staff to update their knowledge. The manual consists of the use of Pensi`s multifunctional stretcher. The Pensi stretcher is a Finnish product and widely used. The manual was generated in co-operation with Pensi Rescue Ltd. There is only a little information on the ergonomics and the use of the stretcher in emergency care training. However, the essential part of work focuses on the use of stretcher`s. The correct handling of the stretcher needs more attention because of the paramedic and the patient safety. Critical care is a heavy duty due to the lifting and the transferring. They require accuracy, confidence, briskness and good planning. The patient might be seriously ill or injured and he/she cannot assist. Furthermore, the environment can be challenging by limited and varied spaces. Therefore, it is essential to learn how to master the handling of the tool and accomplish the task safely. Pensi Rescue Ltd has published a video. The guidebook "Pensiapuaopas" completes this video and serves the educational needs of the personnel working in the 9Lives organization in Sastamala

Genetic diversity of genotype 6 HCV infections in France: Epidemiology and consequences for treatment strategy

International audienceGenotype-6 hepatitis C virus (GT6-HCV) exhibits a high genetic diversity. GT6 prevalence, diversity and real-life response to treatment were studied among 14 603 HCV mono-infected patients from the French ANRS-CO22-Hepather cohort. NS3, NS5A and NS5B-HCV genes were amplified and sequenced for all GT6-infections identified in the database. Following phylogenic characterization, resistance-associated substitution polymorphisms were identified. GT6-infected patients (n = 36; 0.25%) did not differ from patients infected with other genotypes with regard to gender, age or liver fibrosis. GT6e was the most prevalent (27.8%), followed by 6a (22.2%), 6q (11.1%) and 6o (8.3%). Each subtype p and xc were found in two patients (5.6%) and subtypes f/h/r and t were each detected in one patient. Four strains (11.1%) clustered with unclassified reference sequences. Concordant genotype determination throughout NS3, NS5A and NS5B-genes is consistent with lack of recombination within this genomic region. All, but three patients were born in Asia, Cambodia (44.4%), Vietnam (38.9%) or Laos (8.3%). GT6a were found in 42.8% of Vietnamese and 6e in 37.5% of Cambodian. GT6q, 6p and 6r were only found in Cambodian patients. Resistance-associated polymorphisms for each DAA classes were identified in baseline sequences. Twenty-seven patients were treated with sofosbuvir-based combinations and 3 with glecaprevir/pibrentasvir. All treated patients, whether naive or previously treated, achieved a sustained viral response. In conclusion, GT6-infections are uncommon in France and their genetic diversity likely reflects infection within the country of origin. Despite residue variability at DAA resistance-associated positions, sustained viral response was obtained in all treated patients