Hurricane Irma induces divergent behavioral and hormonal impacts on an urban and forest population of invasive Anolis lizards: evidence for an urban resilience hypothesis

Hurricanes can have both profound short-term effects on animal populations and serve as long-term drivers of evolutionary change. Animals inhabiting varying habitats may differ in their response to hurricane impacts. Increasing evidence sug- gests that animals from urban areas exhibit different behavioral and physiological traits compared to rural counterparts, in- cluding attenuated hormonal stress responses and a lowered propensity for flight behavior. A unique opportunity was pre- sented when Hurricane Irma hit Florida on 10 September 2017 and interrupted a study of invasive brown anoles (Anolis sagrei) at an urban and a forest. Using data collected before and after Hurricane Irma, we documented that forest anoles exhibited a greater avoidance of people and more male territorial behavior for a longer period of time following the hurri- cane. Post-hurricane both populations increased corticosterone concentrations post-capture stress, but urban anoles recov- ered 2 weeks faster than forest conspecifics. A dexamethasone suppression experiment suggested that these population dif- ferences were the result of forest anoles having a less effective negative feedback regulating corticosterone secretion. In the brain, forest anoles had higher corticosterone concentrations within the amygdala and parts of the cortex associated with stress than urban lizards. One explanation may be Hurricane Irma brought flooding and debris that altered the landscape leading to behavioral instability, and urban lizards already exhibited ecological adjustments that permitted a more rapid re- covery (i.e. the ‘urban resilience’ hypothesis). Testing if urban animals are more resilient to natural disasters can inform conservationists interested in understanding their role in facilitating invasive species expansion and what their increasing presence may indicate for animal populations

Distribution of Bats in Bottomland Hardwood Forests of the Arkansas Delta Region

Bat distribution data is incomplete for the delta region of Arkansas. We extensively surveyed 16 counties within the Mississippi alluvial plain that comprises the delta from late spring to early fall 2004 using mist nets. We obtained 44 new county records for 9 species: Myotis lucifigus, M. austroriparius, Pipistrellus subflavus, Eptesicus fuscus, Lasiurus seminolus, L. borealis, L. cinereus, Nycticeius humeralis, and Corynorhinus rafinesquii. We generated updated distribution maps for these species and eastward Arkansas range expansions were documented for L.seminolus. Possible sampling concerns and research directions are discussed in relation to the needs of bats inhabiting bottomland forests of the delta, particularly M. austroriparius and C. rafinesquii

Eating increases oxidative damage in a reptile

While eating has substantial benefits in terms of both nutrient and energy acquisition, there are physiological costs associated with digesting and metabolizing a meal. Frequently, these costs have been documented in the context of energy expenditure while other physiological costs have been relatively unexplored. Here, we tested whether the seemingly innocuous act of eating affects either systemic pro-oxidant (reactive oxygen metabolite, ROM) levels or antioxidant capacity of corn snakes (Pantherophis guttatus) by collecting plasma during absorptive (peak increase in metabolic rate due to digestion of a meal) and non-absorptive (baseline) states. When individuals were digesting a meal, there was a minimal increase in antioxidant capacity relative to baseline (4%), but a substantial increase in ROMs (nearly 155%), even when controlling for circulating nutrient levels. We report an oxidative cost of eating that is much greater than that due to long distance flight or mounting an immune response in other taxa. This result demonstrates the importance of investigating non-energetic costs associated with meal processing, and it begs future work to identify the mechanism(s) driving this increase in ROM levels. Because energetic costs associated with eating are taxonomically widespread, identifying the taxonomic breadth of eating-induced ROM increases may provide insights into the interplay between oxidative damage and life history theory

Assessing chronic stress in wild mammals using claw-derived cortisol: a validation using European badgers (Meles meles)

Measuring stress experienced by wild mammals is increasingly important in the context of human-induced rapid environmental change and initiatives to mitigate human-wildlife conflicts. Glucocorticoids (GC), such as cortisol, mediate responses by promoting physiological adjustments during environmental perturbations. Measuring cortisol is a popular technique; however, this often reveals only recent short-term stress such as that incurred by restraining the animal to sample blood, corrupting the veracity of this approach. Here we present a protocol using claw cortisol, compared with hair cortisol, as a long-term stress bio-indicator, which circumvents this constraint, where claw tissue archives the individual's GC concentration over preceding weeks. We then correlate our findings against detailed knowledge of European badger life history stressors. Based on a solid-phase extraction method, we assessed how claw cortisol concentrations related to season and badger sex, age and body-condition using a combination of generalized linear mixed models (GLMM) (n = 668 samples from 273 unique individuals) followed by finer scale mixed models for repeated measures (MMRM) (n = 152 re-captured individuals). Claw and hair cortisol assays achieved high accuracy, precision and repeatability, with similar sensitivity. The top GLMM model for claw cortisol included age, sex, season and the sex*season interaction. Overall, claw cortisol levels were significantly higher among males than females, but strongly influenced by season, where females had higher levels than males in autumn. The top fine scale MMRM model included sex, age and body condition, with claw cortisol significantly higher in males, older and thinner individuals. Hair cortisol was more variable than claw; nevertheless, there was a positive correlation after removing 34 outliers. We discuss strong support for these stress-related claw cortisol patterns from previous studies of badger biology. Given the potential of this technique, we conclude that it has broad application in conservation biology

Urban bat pups take after their mothers and are bolder and faster learners than rural pups.

Background: Urbanization is rapidly changing our planet and animals that live in urban environments must quickly adjust their behavior. One of the most prevalent behavioral characteristics of urban dwelling animals is an increased level of risk-taking. Here, we aimed to reveal how urban fruitbats become risk-takers, and how they differ behaviorally from rural bats, studying both genetic and non-genetic factors that might play a role in the process. We assessed the personality of newborn pups from both rural and urban colonies before they acquired experience outdoors, examining risk-taking, exploration, and learning rates. Results: Urban pups exhibited significantly higher risk-taking levels, they were faster learners, but less exploratory than their rural counterparts. A cross-fostering experiment revealed that pups were more similar to their adoptive mothers, thus suggesting a non-genetic mechanism and pointing towards a maternal effect. We moreover found that lactating urban mothers have higher cortisol levels in their milk, which could potentially explain the transmission of some personality traits from mother to pup. Conclusions: Young bats seem to acquire environment suitable traits via post-birth non-genetic maternal effects. We offer a potential mechanism for how urban pups can acquire urban-suitable behavioral traits through hormonal transfer from their mothers

Up to the challenge?: hormonal and behavioral responses of free-ranging male Cassin's sparrows, Peucaea cassinii, to conspecific song playback

The Challenge Hypothesis postulates that male vertebrates can respond to social challenges, such as simulated territorial intrusions (STI), by rapidly increasing their concentrations of plasma androgens, such as testosterone (T). This increase may facilitate the expression of aggressive behavior and lead to persistence of this behavior even after withdrawal of the challenge, thus potentially promoting territoriality and the probability of winning future challenges. The validity of the Challenge Hypothesis was investigated in socially monogamous free-ranging male Cassin’s Sparrows, Peucaea cassinii. Exposure to STI at the beginning of the vernal nesting season stimulated aggressive behavior but did not increase plasma T. Furthermore, plasma T did not correlate with the duration of exposure to STI and the behavioral response to STI did not differ in males that were challenged a second time shortly after the first challenge. As birds were investigated at a stage of their reproductive cycle when plasma T is presumably seasonally high due to photostimulation, the lack of hormonal response to STI may have been due to the hypothalamo-pituitary-gonadal axis secreting hormones at maximum rates. This was not the case, however, because administration of gonadotropin-releasing hormone I (GnRH-I) rapidly stimulated the secretion of luteinizing hormone (LH) and T, and treatment with ovine LH rapidly stimulated T secretion

Advanced seasonal reproductive development in a male urban bird is reflected in earlier plasma luteinizing hormone rise but not energetic status

AbstractUrban animals inhabit an environment considerably different than do their non-urban conspecifics, and to persist urban animals must adjust to these novel environments. The timing of seasonal reproductive development (i.e., growth of gonads and secondary sex organs) is a fundamental determinant of the breeding period and is frequently advanced in urban bird populations. However, the underlying mechanism(s) by which birds adjust the timing of reproductive development to urban areas remain(s) largely unknown. Here, we compared the timing of vernal reproductive development in free-ranging urban and non-urban male Abert’s Towhees, Melozone aberti, in Phoenix, Arizona, USA, and tested the non-mutually exclusive hypotheses that earlier reproductive development is due to improved energetic status and/or earlier increase in endocrine activity of the reproductive system. We found that urban birds initiated testicular development earlier than non-urban birds, but this disparity was not associated with differences in body condition, fat stores, or innate immune performance. These results provide no support for the hypothesis that energetic constraints are responsible for delayed reproductive development of non-urban relative to urban male Abert’s Towhees. Urban birds did, however, increase their plasma luteinizing hormone, but not plasma testosterone, earlier than non-urban birds. These findings suggest that adjustment to urban areas by Abert’s Towhees involves increases in the endocrine activity of the anterior pituitary gland and/or hypothalamus earlier than non-urban towhees