LESSON STUDY DESIGNED TO INFLUENCE TEACHERS’ KNOWLEDGE AND BELIEFS RELATED TO BOYS AND LITERACY

Teacher knowledge and beliefs related to boys and literacy may contribute to the literacy achievement gap between boys and girls. After a review of literature related to this achievement gap and interventions designed to address it, I determined that boys’ literacy outcomes may be improved through professional development designed to influence teachers’ knowledge and beliefs related to boys and literacy. Ecological systems theory served as the theoretical framework and teacher pedagogical knowledge and skills (TPK&S) the conceptual framework. I conducted a mixed methods case study to explore the effects of professional learning rooted in lesson study on teachers’ knowledge and beliefs related to boys and literacy with the intention of ultimately improving boys’ literacy outcomes. The four participants in the study attended 11 sessions over 14 weeks during which they participated in a lesson study and integrated components of gender-relevant pedagogy and balanced literacy into the lesson they designed. Semi-structured interviews, participant journal entries, a researcher’s diary, field notes, the Beliefs about Boys and Reading Instruction survey, the Knowledge about Boys and Reading Instruction quiz, exit tickets, and an observation protocol were used to assess process and outcome objectives. There was a statistically significant shift in participants’ knowledge, but quantitative measures indicated no definitive changes to beliefs. Qualitative data suggests that participants knowledge and beliefs shifted in the areas of student-centered instructional design, personalization, and student-centered classroom practices, and changes to participants’ knowledge of the gender literacy gap, providing students with choice, and designing for boys and girls were detected

Evaluating measures of optimism and sport confidence

The psychometric properties of the Life Orientation Test-Revised (LOT-R), the Sport Confidence Inventory (SCI), and the Carolina SCI (CSCI) were examined in a study involving 260 athletes. The study aimed to test the dimensional structure, convergent and divergent validity, and invariance over competition level of scores generated by these instruments. Exploratory structural equation modeling (ESEM) supported the measurement models for the SCI and CSCI but favoured a two-dimensional model for the LOT-R. ESEM analyses of the total pool of 33 items indicated satisfactory divergent validity among these optimism and sport confidence measures with the only overlap occurring between the LOT-R and CSCI measures of optimism. The SCI discriminated among athletes participating at different competitive levels and emerged as the most suitable instrument for measuring individual differences in sport confidence. The CSCI emerged as a better measure of optimism in a sporting context than the more general LOT-R

Dopamine transporter endocytic trafficking in striatal dopaminergic neurons: differential dependence on dynamin and the actin cytoskeleton

Dopaminergic signaling profoundly impacts rewarding behaviors, movement, and executive function. The presynaptic dopamine (DA) transporter (DAT) recaptures released DA, thereby limiting synaptic DA availability and maintaining dopaminergic tone. DAT constitutively internalizes and PKC activation rapidly accelerates DAT endocytosis, resulting in DAT surface loss. Longstanding evidence supports PKC-stimulated DAT trafficking in heterologous expression studies. However, PKC-stimulated DAT internalization is not readily observed in cultured dopaminergic neurons. Moreover, conflicting reports implicate both classic and nonclassic endocytic mechanisms mediating DAT trafficking. Prior DAT trafficking studies relied primarily upon chronic gene disruption and dominant-negative protein expression, or were performed in cell lines and cultured neurons, yielding results difficult to translate to adult dopaminergic neurons. Here, we use newly described dynamin inhibitors to test whether constitutive and PKC-stimulated DAT internalization are dynamin-dependent in adult dopaminergic neurons. Ex vivo biotinylation studies in mouse striatal slices demonstrate that acute PKC activation drives native DAT surface loss, and that surface DAT surprisingly partitions between endocytic-willing and endocytic-resistant populations. Acute dynamin inhibition reveals that constitutive DAT internalization is dynamin-independent, whereas PKC-stimulated DAT internalization is dynamin-dependent. Moreover, total internal reflection fluorescence microscopy experiments demonstrate that constitutive DAT internalization occurs equivalently from lipid raft and nonraft microdomains, whereas PKC-stimulated DAT internalization arises exclusively from lipid rafts. Finally, DAT endocytic recycling relies on a dynamin-dependent mechanism that acts in concert with the actin cytoskeleton. These studies are the first comprehensive investigation of native DAT trafficking in ex vivo adult neurons, and reveal that DAT surface dynamics are governed by complex multimodal mechanisms

Toward cyberinfrastructure to facilitate collaboration and reproducibility for marine integrated ecosystem assessments

Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Earth Science Informatics 10 (2017): 85-97, doi:10.1007/s12145-016-0280-4.There is a growing need for cyberinfrastructure to support science-based decision making in management of natural resources. In particular, our motivation was to aid the development of cyberinfrastructure for Integrated Ecosystem Assessments (IEAs) for marine ecosystems. The IEA process involves analysis of natural and socio-economic information based on diverse and disparate sources of data, requiring collaboration among scientists of many disciplines and communication with other stakeholders. Here we describe our bottom-up approach to developing cyberinfrastructure through a collaborative process engaging a small group of domain and computer scientists and software engineers. We report on a use case evaluated for an Ecosystem Status Report, a multi-disciplinary report inclusive of Earth, life, and social sciences, for the Northeast U.S. Continental Shelf Large Marine Ecosystem. Ultimately, we focused on sharing workflows as a component of the cyberinfrastructure to facilitate collaboration and reproducibility. We developed and deployed a software environment to generate a portion of the Report, retaining traceability of derived datasets including indicators of climate forcing, physical pressures, and ecosystem states. Our solution for sharing workflows and delivering reproducible documents includes IPython (now Jupyter) Notebooks. We describe technical and social challenges that we encountered in the use case and the importance of training to aid the adoption of best practices and new technologies by domain scientists. We consider the larger challenges for developing end-to-end cyberinfrastructure that engages other participants and stakeholders in the IEA process.Support for this research was provided by the U. S. National Science Foundation #0955649 with additional support to SB by the Investment in Science Fund at Woods Hole Oceanographic Institution

3C 273 Host Galaxy with Hubble Space Telescope Coronagraphy

The close-in regions of bright quasars' host galaxies have been difficult to image due to the overwhelming light from the quasars. With coronagraphic observations in visible light using the Space Telescope Imaging Spectrograph (STIS) on the Hubble Space Telescope, we removed 3C 273 quasar light using color-matching reference stars. The observations revealed the host galaxy from 60" to 0.2" with nearly full angular coverage. Isophote modeling revealed a new core jet, a core blob, and multiple smaller-scale blobs within 2.5". The blobs could potentially be satellite galaxies or infalling materials towards the central quasar. Using archival STIS data, we constrained the apparent motion of its large scale jets over a 22 yr timeline. By resolving the 3C 273 host galaxy with STIS, our study validates the coronagraph usage on extragalactic sources in obtaining new insights into the central ~kpc regions of quasar hosts.Comment: 13 pages, 11 figures, 2 tables, A&A Letters accepte

Impact of Eastern Redcedar Proliferation on Water Resources in the Great Plains USA—Current State of Knowledge

In the Great Plains of the central United States, water resources for human and aquatic life rely primarily on surface runoff and local recharge from rangelands that are under rapid transformation to woodland by the encroachment of Eastern redcedar (redcedar; Juniperus virginiana) trees. In this synthesis, the current understanding and impact of redcedar encroachment on the water budget and water resources available for non-ecosystem use are reviewed. Existing studies concluded that the conversion from herbaceous-dominated rangeland to redcedar woodland increases precipitation loss to canopy interception and vegetation transpiration. The decrease of soil moisture, particularly for the subsurface soil layer, is widely documented. The depletion of soil moisture is directly related to the observed decrease in surface runoff, and the potential of deep recharge for redcedar encroached watersheds. Model simulations suggest that complete conversion of the rangelands to redcedar woodland at the watershed and basin scale in the South-central Great Plains would lead to reduced streamflow throughout the year, with the reductions of streamflow between 20 to 40% depending on the aridity of the climate of the watershed. Recommended topics for future studies include: (i) The spatial dynamics of redcedar proliferation and its impact on water budget across a regional hydrologic network; (ii) the temporal dynamics of precipitation interception by the herbaceous canopy; (iii) the impact of redcedar infilling into deciduous forests such as the Cross Timbers and its impact on water budget and water availability for non-ecosystem use; (iv) land surface and climate interaction and cross-scale hydrological modeling and forecasting; (v) impact of redcedar encroachment on sediment production and water quality; and (vi) assessment and efficacy of different redcedar control measures in restoring hydrological functions of watershed

A randomized, seven-day study to assess the efficacy and safety of a glycopyrrolate/formoterol fumarate fixed-dose combination metered dose inhaler using novel Co-Suspension™ Delivery Technology in patients with moderate-to-very severe chronic obstructive pulmonary disease

Abstract Background Long-acting muscarinic antagonist/long-acting β 2 -agonist combinations are recommended for patients whose chronic obstructive pulmonary disease (COPD) is not managed with monotherapy. We assessed the efficacy and safety of glycopyrrolate (GP)/formoterol fumarate (FF) fixed-dose combination delivered via a Co-Suspension™ Delivery Technology-based metered dose inhaler (MDI) (GFF MDI). Methods This was a Phase IIb randomized, multicenter, placebo-controlled, double-blind, chronic-dosing (7 days), crossover study in patients with moderate-to-very severe COPD (NCT01085045). Treatments included GFF MDI twice daily (BID) (GP/FF 72/9.6 μg or 36/9.6 μg), GP MDI 36 μg BID, FF MDI 7.2 and 9.6 μg BID, placebo MDI, and open-label formoterol dry powder inhaler (FF DPI) 12 μg BID or tiotropium DPI 18 μg once daily. The primary endpoint was forced expiratory volume in 1 s area under the curve from 0 to 12 h (FEV 1 AUC 0–12 ) on Day 7 relative to baseline FEV 1 . Secondary endpoints included pharmacokinetics and safety. Results GFF MDI 72/9.6 μg or 36/9.6 μg led to statistically significant improvements in FEV 1 AUC 0–12 after 7 days’ treatment versus monocomponent MDIs, placebo MDI, tiotropium, or FF DPI (p ≤ 0.0002). GFF MDI 36/9.6 μg was non-inferior to GFF MDI 72/9.6 μg and monocomponent MDIs were non-inferior to open-label comparators. Pharmacokinetic results showed glycopyrrolate and formoterol exposure were decreased following administration via fixed-dose combination versus monocomponent MDIs; however, this was not clinically meaningful. GFF MDI was well tolerated. Conclusions GFF MDI 72/9.6 μg and 36/9.6 μg BID improve lung function and are well tolerated in patients with moderate-to-very severe COPD. Trial registration ClinicalTrials.gov NCT01085045. Registered 9 March 2010

Study protocol: can a school gardening intervention improve children's diets?

BACKGROUND: The current academic literature suggests there is a potential for using gardening as a tool to improve children's fruit and vegetable intake. This study is two parallel randomised controlled trials (RCT) devised to evaluate the school gardening programme of the Royal Horticultural Society (RHS) Campaign for School Gardening, to determine if it has an effect on children's fruit and vegetable intake. METHOD/DESIGN: Trial One will consist of 26 schools; these schools will be randomised into two groups, one to receive the intensive intervention as "Partner Schools" and the other to receive the less intensive intervention as "Associate Schools". Trial Two will consist of 32 schools; these schools will be randomised into either the less intensive intervention "Associate Schools" or a comparison group with delayed intervention. Baseline data collection will be collected using a 24-hour food diary (CADET) to collect data on dietary intake and a questionnaire exploring children's knowledge and attitudes towards fruit and vegetables. A process measures questionnaire will be used to assess each school's gardening activities. DISCUSSION: The results from these trials will provide information on the impact of the RHS Campaign for School Gardening on children's fruit and vegetable intake. The evaluation will provide valuable information for designing future research in primary school children's diets and school based interventions. TRIAL REGISTRATION: ISRCTN11396528

DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos

Deletion of S phase disrupts mitotic timing in maternally regulated cycles, but it doesn't alter the cell cycle once zygotic transcription has begun