Determinación no destructiva de parámetros de calidad de frutas y hortalizas mediante espectroscopía de reflectancia en el infrarrojo cercano

El objetivo general de la presente Tesis Doctoral era el desarrollo y evaluación de una metodología de control analítico rápida, económica, no contaminante y precisa, basada en la aplicación de la Tecnología NIRS, para el control de calidad de forma no destructiva de frutas frescas de pepita y hueso En base a ello, se puso a punto la metodología adecuada para llevar a cabo el análisis NIRS para la predicción de la composición química, y de parámetros de calidad físicos como la textura (firmeza), el peso y el calibre de dichos tipos de fruta, en forma intacta. La posibilidad de conocer si el análisis NIRS de frutas de forma intacta era viable reviste gran importancia por la posibilidad de incrementar la rapidez de los controles y agilizar la toma de decisiones tanto a nivel de campo (momento óptimo de cosecha) como a nivel de industria transformadora. Se desarrollaron asimismo, modelos NIRS de predicción cualitativos destinados a la determinación de tiempos de almacenamiento poscosecha en refrigeración y en estantería con el fin de establecer la vida útil o comercial de frutas frescas. Finalmente, se han seleccionado para las distintas aplicaciones desarrolladas en este Trabajo de Investigación los instrumentos NIRS más idóneos en función tanto de las necesidades del campo y de la industria de transformación como de los parámetros de interés elegidos y de las características intrínsecas de las frutas de pepita y hueso estudiadas. Los resultados obtenidos muestran que la tecnología NIRS se puede configurar como una técnica de análisis instantánea destinada a la determinación de la calidad y vida comercial de frutas frescas de pepita y de hueso en forma intacta e individualizada, posibilitando el análisis de lotes completos de producció

Commentary: Consumer Reports of "Keto Flu" Associated With the Ketogenic Diet

CIBER Fisiopatología Obesidad y Nutrición (CIBEROBN) Consorcio Centro de Investigación Biomédica en Red, M.P. (CIBER) Instituto de Salud Carlos II

Apreciación de la estética dental e impacto psicosocial asociados a la presencia de alteraciones cromáticas y de oclusión en jóvenes estudiantes de ciencias de la salud, Lima – 2017

Objective. To establish the association between the appreciation and psychosocial impact of dental aesthetics with the presence of chromatic alterations and occlusion in young students of health sciences. Material and methods. The study was prospective, cross-sectional, observational and correlational. The sample size was 326 students from 16 to 28 years of age belonging of the Federico Villarreal National University. The subjective evaluations were measured through the Psychosocial Impact Questionnaire of the dental aesthetics (PIDAQ) and for the appreciation, dental photographs of the previous sector were taken and these were presented on a digital tablet, where the participants rated the aesthetics of the color and position of your teeth by Likert scal. Likewise, alterations in occlusion were evaluated using the Dental Aesthetic Index (DAI) and chromatic alterations, caused by enamel defects, using the Enamel Defects Index (IDDEm). Results. The chromatic characteristics, of irregularity and dental occlusion on the esthetic appreciation, were statistically significant (p<0.01); however, with the characteristics of spacing, not association was found (p=1.00). The PIDAQ increased with the DAI score, showing a very significant positive relationship (p<0.01). The IDDEm with PIDAQ, there was an association (p<0.01) except for the "Social" dimension. Conclusion. Minor irregularities are better accepted; as these are more severe, an unpleasant appreciation increases, generating even a greater psychosocial impact on the quality of life.Objetivo. Establecer la asociación entre la apreciación e impacto psicosocial de la estética dental con la presencia de alteraciones cromáticas y de oclusión en jóvenes estudiantes de ciencias de la salud. Material y métodos. El estudio fue prospectivo, transversal, observacional y correlacional. La muestra fue de 326 estudiantes de 16 a 28 años de edad pertenecientes a la Universidad Nacional Federico Villarreal. Las evaluaciones subjetivas fueron medidas a través del Cuestionario de Impacto psicosocial de la estética dental (PIDAQ) y para la apreciación, se les tomaron fotografías dentales del sector anterior y éstas se presentaron en una tableta digital, donde los participantes calificaron la estética del color y posición de sus dientes mediante escala Likert. Así mismo se evaluó alteraciones de oclusión mediante el Índice de Estética dental (DAI) y alteraciones cromáticas, causadas por defectos del esmalte, mediante el Índice de Defectos del Esmalte (IDDEm). Resultados. Las características cromáticas, de irregularidad y oclusión dental sobre la apreciación estética, fueron estadísticamente significativos (p<0.01); no obstante, con las características de espaciamiento no se halló asociación (p=1.00). El PIDAQ aumento con el puntaje DAI, mostrando una relación positiva muy significativa (p<0.01). El IDDEm con PIDAQ, hubo asociación (p<0.01) excepto con la dimensión “Social”. Conclusión. Las irregularidades mínimas son mejor aceptadas; a medida que éstas son más severas, aumenta una apreciación desagradable generando incluso un mayor impacto psicosocial en la calidad de vida

Autism Spectrum Disorder (ASD) with and without Mental Regression Is Associated with Changes in the Fecal Microbiota

New microbiome sequencing technologies provide novel information about the potential interactions among intestinal microorganisms and the host in some neuropathologies as autism spectrum disorders (ASD). The microbiota–gut–brain axis is an emerging aspect in the generation of autistic behaviors; evidence from animal models suggests that intestinal microbial shifts may produce changes fitting the clinical picture of autism. The aim of the present study was to evaluate the fecal metagenomic profiles in children with ASD and compare them with healthy participants. This comparison allows us to ascertain how mental regression (an important variable in ASD) could influence the intestinal microbiota profile. For this reason, a subclassification in children with ASD by mental regression (AMR) and no mental regression (ANMR) phenotype was performed. The present report was a descriptive observational study. Forty-eight children aged 2–6 years with ASD were included: 30 with ANMR and 18 with AMR. In addition, a control group of 57 normally developing children was selected andmatched to the ASD group by sex and age. Fecal samples were analyzed with a metagenomic approach using a next-generation sequencing platform. Several differences between children with ASD, compared with the healthy group, were detected. Namely, Actinobacteria and Proteobacteria at phylum level, as well as, Actinobacteria, Bacilli, Erysipelotrichi, and Gammaproteobacteria at class level were found at higher proportions in children with ASD. Additionally, Proteobacteria levels showed to be augmented exclusively in AMR children. Preliminary results, using a principal component analysis, showed differential patterns in children with ASD, ANMR and AMR, compared to healthy group, both for intestinal microbiota and food patterns. In this study, we report, higher levels of Actinobacteria, Proteobacteria and Bacilli, aside from Erysipelotrichi, and Gammaproteobacteria in children with ASD compared to healthy group. Furthermore, AMR children exhibited higher levels of Proteobacteria. Further analysis using these preliminary results and mixing metagenomic and other “omic” technologies are needed in larger cohorts of children with ASD to confirm these intestinal microbiota changes.This study was supported by the FUNDACIÓ AGRUPACIÓ Àmbit de la Infància, 404 Research Grant INVEST from the Spanish Society of Pediatrics and Red de Salud Materno Infantil (RED SAMID). The funding bodies did not have any role in the design, collection, analyses, or interpretation of data or in writing the manuscript.Julio Plaza-Diaz is part of University of Granada, Plan Propio de Investigación 2016, Excellence actions: Units of Excellence; Unit of Excellence on Exercise and Health (UCEES). Patricio Solis-Urra was supported by a grant from CONICYT/BECAS Chile/72180543

Evaluation of the safety, tolerance and efficacy of 1-year consumption of infant formula supplemented with Lactobacillus fermentum CECT5716 Lc40 or Bifidobacterium breve CECT7263: a randomized controlled trial

Background: The microorganism present in breast milk, added to other factors, determine the colonization of infants. The objective of the present study is to evaluate the safety, tolerance and effects of the consumption of a milk formula during the first year of life that is supplemented with L. fermentum CECT5716 or Bifidobacterium breve CECT7263, two strains originally isolated from breast milk. Results: One hundred and eighty-nine infants completed the eleven months of intervention (61 in control group, 65 in Lf group and 63 in Bb group). The growth of infants in the three groups was consistent with standards. No significant differences were observed in the main outcome, weight-gain (Control group: 5.77 Kg ± 0.95, Lf group: 5.77 Kg ± 1.31, Bb group: 5.58 Kg ± 1.10; p = 0.527). The three milk formulae were well tolerated, and no adverse effects were related to the consumption of any of the formula. Infants receiving B. breve CECT7263 had a 1.7 times lower risk of crying than the control group (OR = 0.569, CI 95% 0.568–0.571; p = 0.001). On the other hand, the incidence of diarrhoea in infants receiving the formula supplemented with L. fermentum CECT5716 was a 44% lower than in infants receiving the control formula (p = 0.014). The consumption of this Lactobacillus strain also reduced the duration of diarrhoea by 2.5 days versus control group (p = 0.044). Conclusions: The addition of L. fermentum CECT5716 Lc40 or B. breve CECT7263, two probiotic strains naturally found in breast milk, to infant formulae is safe and induces beneficial effects on the health of infants.This work was funded by Biosearch Life supported by a grant from the Agency of Innovation and Development of Andalusia (IDEA-Spain), Cofinanced by European Regional Development Fund (EC). Project Tittle: “New applications of probiotic strains and derived compounds with biological activity (POSTBIO)” and partially funded by Lactalis-Puleva (Granada, Spain)

Longitudinal associations between cardiovascular biomarkers and metabolic syndrome during puberty: the PUBMEP study

Puberty has been described as a life stage of considerable metabolic risk specially for those with obesity. The low-grade systemic inflammatory status associated with obesity could be one of the connections with metabolic syndrome (MetS). Thus, we aimed to assess the relationship between inflammatory and cardiovascular biomarkers and the development of MetS during puberty. Seventy-five children from the PUBMEP study (33 females), aged 4–18 years, were included. Cardiovascular and inflammatory biomarkers were measured in the prepubertal and pubertal stage, including high-sensitivity C-reactive protein (CRP), leptin, tumor necrosis factor-alpha (TNFα), interleukin 8 (IL8), monocyte chemoattractant protein 1 (MCP-1), total plasminogen activator inhibitor-1 (tPAI), resistin, adiponectin, myeloperoxidase (MPO), and soluble intercellular adhesion molecule-1 (sICAM-1). MetS was diagnosed at each measurement point. Mixed-effects and logistic regressions were performed. Those children with MetS in puberty presented higher prepubertal values of several cardiometabolic biomarkers in comparison to those without MetS (z-score body mass index (zBMI), waist circumference, insulin, HOMA-IR, leptin, and tPAI (p < 0.05)). For prepubertal children with obesity, the odds of developing MetS in puberty were significantly higher in those having high zBMI (OR = 4.27; CI: 1.39–22.59) or high concentrations of tPAI (OR = 1.19; CI: 1.06–1.43)Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by the Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + I), Instituto de Salud Carlos III-Health Research Funding (FONDOS FEDER) (PI11/01425, PI11/02042, PI11/02059, PI16/01301, PI16/01205, PI16/00871 and PI20/00563); CIBEROBN Network (CB15/00131, CB15/00043); and Redes temáticas de investigación cooperativa RETIC (Red SAMID RD12/0026/0015)S

Longitudinal associations between cardiovascular biomarkers and metabolic syndrome during puberty: the PUBMEP study

Puberty has been described as a life stage of considerable metabolic risk specially for those with obesity. The low-grade systemic inflammatory status associated with obesity could be one of the connections with metabolic syndrome (MetS). Thus, we aimed to assess the relationship between inflammatory and cardiovascular biomarkers and the development of MetS during puberty. Seventy-five children from the PUBMEP study (33 females), aged 4–18 years, were included. Cardiovascular and inflammatory biomarkers were measured in the prepubertal and pubertal stage, including high-sensitivity C-reactive protein (CRP), leptin, tumor necrosis factor-alpha (TNFα), interleukin 8 (IL8), monocyte chemoattractant protein 1 (MCP-1), total plasminogen activator inhibitor-1 (tPAI), resistin, adiponectin, myeloperoxidase (MPO), and soluble intercellular adhesion molecule-1 (sICAM-1). MetS was diagnosed at each measurement point. Mixed-effects and logistic regressions were performed. Those children with MetS in puberty presented higher prepubertal values of several cardiometabolic biomarkers in comparison to those without MetS (z-score body mass index (zBMI), waist circumference, insulin, HOMA-IR, leptin, and tPAI (p < 0.05)). For prepubertal children with obesity, the odds of developing MetS in puberty were significantly higher in those having high zBMI (OR = 4.27; CI: 1.39–22.59) or high concentrations of tPAI (OR = 1.19; CI: 1.06–1.43)Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by the Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + I), Instituto de Salud Carlos III-Health Research Funding (FONDOS FEDER) (PI11/01425, PI11/02042, PI11/02059, PI16/01301, PI16/01205, PI16/00871 and PI20/00563); CIBEROBN Network (CB15/00131, CB15/00043); and Redes temáticas de investigación cooperativa RETIC (Red SAMID RD12/0026/0015)S

Impaired Antioxidant Defence Status Is Associated With Metabolic-Inflammatory Risk Factors in Preterm Children With Extrauterine Growth Restriction: The BIORICA Cohort Study

Introduction: An impaired antioxidant status has been described during foetal growth restriction (FGR). Similarly, the antioxidant defence system can be compromised in preterm children with extrauterine growth restriction (EUGR). The aim of this prospective study was to evaluate the antioxidant status in prepubertal children with a history of prematurity without FGR, with and without EUGR, compared to a healthy group. Methods: In total, 211 children were recruited and classified into three groups: 38 with a history of prematurity and EUGR; 50 with a history of prematurity and adequate extrauterine growth (AEUG); and 123 control children born at term. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) activities were assessed in lysed erythrocytes with spectrophotometric methods. Plasma levels of the antioxidants α-tocopherol, retinol and β-carotene were determined through solvent extraction and ultra-high-pressure liquid chromatography coupled to mass spectrometry. Results: Children with the antecedent of EUGR and prematurity had lower CAT activity than the other two groups and lower GPx activity than the control children. Lower SOD, GPx and GR activities were observed in the AEUG group compared to the controls. However, higher concentrations of α-tocopherol and β-carotene were found in the EUGR group compared to the other groups; retinol levels were also higher in EUGR than in AEUG children. In EUGR and AEUG children, enzymatic antioxidant activities and plasma antioxidants were associated with metabolic syndrome components and pro-inflammatory biomarkers. Conclusions: This study reveals, for the first time, that the EUGR condition and prematurity appear to be linked to an impairment of the antioxidant defence status, which might condition an increased risk of adverse metabolic outcomes later in life.This study was funded by the Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (Iþ DþI), Instituto de Salud Carlos III-Fondo de Investigación Sanitaria Project No. PI13/01245 from the Spanish Ministry of Health and Consumer Affairs and co-financed by the Consejería de Innovación y Ciencia, Junta de Andalucía, PI-0480-2012, Spain. ÁG was funded by the Research Plan of the Vice-Rectorate of Research and Transfer of the University of Granada, Spain. This paper will be included in MO-D's doctorate under the Biomedicine Program at the University of Córdoba, Spain. The funding bodies did not partake in the design, collection, analyses, or interpretation of the data or in writing the manuscript. Maternal-Infant and Developmental Health Network (SAMID), RETICS Carlos III Health Institute (ISCIII), Madrid, Spain (Red SAMID RD12/0026/0015).Ye

Corrigendum: Reliability and validation of the child eating behavior questionnaire in 3- to 6-year-old Spanish children

A corrigendum on Reliability and validation of the child eating behavior questionnaire in 3- to 6-year-old Spanish children by Jimeno-Martínez, A., Maneschy, I., Moreno, L. A., Bueno-Lozano, G., De Miguel-Etayo, P., Flores-Rojas, K., Jurado-Castro, J. M., de Lamas, C., Vázquez-Cobela, R., Martinez-Lacruz, R., Portoles, O., Martínez, J. A., Navas-Carretero, S., Schröder, H., Fitó, M., Babio, N., Salas-Salvadó, J., Leis, R., Gil-Campos, M., and Rupérez, A. I. (2022). Front. Psychol. 13:705912. doi: 10.3389/fpsyg.2022.70591

Predicción de color de la pulpa en melones enteros mediante espectroscopía de reflectancia en el infrarrojo cercano

El color de la pulpa del melón es uno de los índices utilizados para determinar el grado de madurez y calidad del mismo, siendo necesario disponer de medidas no destructivas que impidan el daño y la depreciación del producto. La tecnología NIRS ofrece enormes expectativas en este terreno, particularmente derivadas de su carácter de no destructiva, rápida y con posibilidad de ser incorporada a nivel de la línea de producción. El objetivo de este trabajo de investigación fue evaluar un instrumento NIRS de red de diodos para estimar el color del mesocarpio (a*: variación verde-rojo y b*: variación amarillo-azul) de melones intactos. El desarrollo de los modelos de predicción de color se realizó utilizando un colectivo constituido por 158 muestras (vars. Cantaloupe y Galia) procedentes de El Ejido (Almería). Los valores del coeficiente de determinación (r2) y del error típico de validación cruzada (ETVC) obtenidos para los parámetros a* (0,97, 1,98) y b* (0,88, 3,17) en la fruta intacta indican la viabilidad inicial del empleo de la tecnología NIRS para la determinación de la madurez y calidad de frutos de melón enteros