Effect of three commercial mouth rinses on cultured human gingival fibroblast: An <i> in vitro</i> study

<b>Aim:</b> To examine the effect of three commercial mouth rinses (Hexidine 0.2&#x0025;, Listerine Cool Mint, Betadine 1&#x0025;) upon cultured human gingival fibroblast proliferation. <b> Materials and Methods:</b> Human gingival fibroblasts were cultured and incubated in Dulbecco&#x2032;s Minimum Eagle&#x2032;s Medium containing Chlorhexidine, Listerine, Povidone-Iodine at varying concentrations (1&#x0025;, 2&#x0025;, 5&#x0025;, 10&#x0025;, 20&#x0025; and 100&#x0025; of the given solution) at 37&#x00B0;C for 1, 5 and 15 min. Control cells received an equal volume of Dulbecco&#x2032;s Minimum Eagle&#x2032;s Medium without adding mouth rinses, for similar duration of exposure at 37&#x00B0;C. Following incubation the media were removed, cells were washed twice with medium, supplemented with 10&#x0025; Fetal Bovine Serum, and fibroblasts in the test and control group were allowed to recover in the same media for 24 h. <b> Results:</b> In all the three groups, the proliferation inhibition was dependent on the concentration of solublized mouth rinses in the cell culture but independent of the duration of exposure to all three mouth rinses. The results showed that all three solutions were toxic to cultured human gingival fibroblasts, Chlorhexidine being the most cytotoxic. It was seen that at dilute concentrations (1&#x0025; and 2&#x0025; of given solutions) Listerine was more cytotoxic than Chlorhexidine and Povidone-Iodine. <b> Conclusion:</b> These results suggest that Chlorhexidine, Listerine and Povidone-Iodine are capable of inducing a dose-dependent reduction in cellular proliferation of fibroblasts. The results presented are interesting, but to know the clinical significance, further studies are needed

Fibrin glue application in conjunction with tetracycline hydrochloride root conditioning and semilunar coronally repositioned flap in the treatment of gingival recession

The purpose of this case report is to present the results of Fibrin Adhesive System (FAS) application, a topical biological tissue adhesive in the treatment of maxillary buccal recessions. A 40-year-old male patient presented with a pair of class I buccal recession defects on maxillary cuspids. Clinical parameters were recorded at baseline, 1 month, and 3 months. Semilunar coronally repositioned flap (Tarnow's technique), root debridement, root conditioning with tetracycline hydrochloride solution and one of the defect was treated with FAS application. Significant recession, height reduction, and attachment gain were observed with the FAS application defect site. About 50% of root coverage was noted in the FAS-treated defect and almost no root coverage in the defect site with no FAS application. Semilunar coronally repositioned flap with FAS application is an effective procedure for root coverage in anterior teeth. The addition of FAS improves the amount of root coverage, especially in relatively shallow defects