49 research outputs found
Streptococcus parasanguinis: new pathogen associated with asymptomatic mastitis in sheep.
We describe two unusual cases in sheep of subclinical mastitis caused by Streptococcus parasanguinis. This bacterium has been associated with the development of experimental endocarditis; its presence at relatively high concentrations in apparently healthy sheep milk may pose a health risk in persons with predisposing heart lesions
Lactococcus lactis subsp. lactis infection in waterfowl: first confirmation in animals.
We report the first description, confirmed by bacteriologic and molecular (polymerase chain reaction and pulsed-field gel electrophoresis) analysis, of an infection in animals caused by Lactococcus lactis subsp. lactis, affecting waterfowl
Multi-Etiological Nature of Tuberculosis-Like Lesions in Condemned Pigs at the Slaughterhouse
Tuberculosis-like lesions (TBL) in pigs have been associated with microorganisms other
than mycobacteria. In this work a histopathological and microbiological evaluation of TBL in
pigs is shown. A total of 352 samples belonging to 171 pigs totally condemned at slaughterhouse
due to generalized TBL were sampled and selected for analysis. Pyogranulomatous
(56.2%) and granulomatous lesions (20.2%) were observed in all analysed organs. Most of
the granulomas observed in both lymph nodes and lungs belonged to more advanced
stages of development (stages III and IV) whereas in the liver and the spleen most of lesions
belonged to intermediate stages (stages II and III). Different microorganisms were simultaneously
detected from TBL in the 42.7% of the animals. Mycobacterium tuberculosis complex
(MTC) (38%), coryneform bacteria (40.3%) and streptococci (28.1%) were the main
groups of microorganisms detected after bacteriological analysis, with Trueperella pyogenes
and Streptococcus suis as the most frequently isolated species. Mycobacteria
belonging to MTC were the most frequently detected pathogens in granulomatous and pyogranulomatous
lesions in submandibular lymph nodes (32.7%) and coryneform bacteria
were the microorganisms more frequently isolated from lungs (25.9%) and spleen samples
(37.2%). These results may provide new insights into the pathogenesis and diagnosis of
this pathology. The importance of coryneform bacteria and streptococci in such processes
must be evaluated in future studie
Isolation of Corynebacterium xerosis from Animal Clinical Specimens
This article describes the first identification of Corynebacterium xerosis from animal clinical specimens, which was confirmed by microbiological and molecular genetic (16S rRNA gene sequencing) methods
Multiplex PCR Assay for Detection of Bacterial Pathogens Associated with Warm-Water Streptococcosis in Fish
A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Streptococcus parauberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex PCR using purified DNA was 25 pg for S. iniae, 12.5 pg for S. difficilis, 50 pg for S. parauberis, and 30 pg for L. garvieae. The multiplex PCR assay was useful for the specific detection of the four species of bacteria not only in pure culture but also in inoculated fish tissue homogenates and naturally infected fish. Therefore, this method could be a useful alternative to the culture-based method for the routine diagnosis of warm-water streptococcal infections in fish
Characterization of Aerococcus viridans Isolates from Swine Clinical Specimens▿
We present here the biochemical and genetic characterization and antimicrobial susceptibility of 58 isolates of Aerococcus viridans isolated in pure culture from different clinical specimens of normally sterile body sites of pigs. A. viridans isolates were commonly susceptible to β-lactam antimicrobials and exhibited a great genetic heterogeneity as determined by pulsed-field gel electrophoresis typing. The results indicate that A. viridans might be included in the list of possible etiological agents causing disease in pigs