Expression profiling of metalloproteinases and tissue inhibitors of metalloproteinases in normal and degenerate human achilles tendon

To profile the messenger RNA (mRNA) expression for the 23 known genes of matrix metalloproteinases (MMPs), 19 genes of ADAMTS, 4 genes of tissue inhibitors of metalloproteinases (TIMPs), and ADAM genes 8, 10, 12, and 17 in normal, painful, and ruptured Achilles tendons. Tendon samples were obtained from cadavers or from patients undergoing surgical procedures to treat chronic painful tendinopathy or ruptured tendon. Total RNA was extracted and mRNA expression was analyzed by quantitative real-time reverse transcription–polymerase chain reaction, normalized to 18S ribosomal RNA. In comparing expression of all genes, the normal, painful, and ruptured Achilles tendon groups each had a distinct mRNA expression signature. Three mRNA were not detected and 14 showed no significant difference in expression levels between the groups. Statistically significant (P < 0.05) differences in mRNA expression, when adjusted for age, included lower levels of MMPs 3 and 10 and TIMP-3 and higher levels of ADAM-12 and MMP-23 in painful compared with normal tendons, and lower levels of MMPs 3 and 7 and TIMPs 2, 3, and 4 and higher levels of ADAMs 8 and 12, MMPs 1, 9, 19, and 25, and TIMP-1 in ruptured compared with normal tendons. The distinct mRNA profile of each tendon group suggests differences in extracellular proteolytic activity, which would affect the production and remodeling of the tendon extracellular matrix. Some proteolytic activities are implicated in the maintenance of normal tendon, while chronically painful tendons and ruptured tendons are shown to be distinct groups. These data will provide a foundation for further study of the role and activity of many of these enzymes that underlie the pathologic processes in the tendon

The Paradox of Parkour: Conformity, Resistance and Spatial Exclusion

Drawing upon two years of ethnographic research into the spatially transgressive practice of parkour and freerunning, this chapter attempts to explain and untangle some of the contradictions that surround this popular lifestyle sport and its exclusion from our hyper-regulated cities. While the existing criminological wisdom suggests that these practices are a form of politicised resistance, this chapter positions parkour and freerunning as hyper-conformist to the underlying values of consumer capitalism and explains how late capitalism has created a contradiction for itself in which it must stoke desire for these lifestyle practices whilst also excluding their free practice from central urban spaces. Drawing on the emergent deviant leisure perspective’s interest in issues of infantilisation and adultification, this chapter explores the lifeworlds of young people who are attempting to navigate the challenges and anxieties of early adulthood. For the young people in this study, consumer capitalism’s commodification of rebellious iconography offered unique identities of ‘cool individualism’ and opportunities for flexibilised employment, while the post-industrial ‘creative city’ attempted to harness parkour’s practice, prohibitively if necessary, into approved spatial contexts under the buzzwords of ‘culture’ and ‘creativity’. Therefore, this chapter engages in a critical criminological reappraisal of issues of transgression, deviance and resistance in urban space under consumer capitalism

Rare Cause of a Chiasmal Syndrome

A 44-year old woman with visual loss, fatigue, weight loss, headache and personality change.20/400 OD, Counting fingers OSSuprasellar vascular mas

Model-Based Insights into Multi-Host Transmission and Control of Schistosomiasis

The authors discuss a new study that helps to elucidate the determinants of schistosomiasis transmission in an endemic area of the Philippines