2 research outputs found
Heteromultivalent DNA Enhances the Assembly Yield of Hybrid Nanoparticles and Facilitates Dynamic Disassembly for Bioanalysis Using ICP–MS
To
obtain enhanced physical and biological properties, various
nanoparticles are typically assembled into hybrid nanoparticles through
the binding of multiple homologous DNA strands to their complementary
counterparts, commonly referred to as homomultivalent assembly. However,
the poor binding affinity and limited controllability of homomultivalent
disassembly restrict the assembly yield and dynamic functionality
of the hybrid nanoparticles. To achieve a higher binding affinity
and flexible assembly choice, we utilized the paired heteromultivalency
DNA to construct hybrid nanoparticles and demonstrate their excellent
assembly characteristics and dynamic applications. Specifically, through
heteromultivalency, DNA-functionalized magnetic beads (MBs) and gold
nanoparticles (AuNPs) were efficiently assembled. By utilizing ICP–MS,
the assembly efficiency of AuNPs on MBs was directly monitored, enabling
quantitative analysis and optimization of heteromultivalent binding
events. As a result, the enhanced assembly yield is primarily attributed
to the fact that heteromultivalency allows for the maximization of
effective DNA probes on the surface of nanoparticles, eliminating
steric hindrance interference. Subsequently, with external oligonucleotides
as triggers, it was revealed that the disassembly mechanism of hybrid
nanoparticles was initiated, which was based on an increased local
concentration rather than toehold-mediated displacement of paired
heteromultivalency DNA probes. Capitalizing on these features, an
output platform was then established based on ICP–MS signals
that several Boolean operations and analytical applications can be
achieved by simply modifying the design sequences. The findings provide
new insights into DNA biointerface interaction, with potential applications
to complex logic operations and the construction of large DNA nanostructures
Data_Sheet_1_Association between microbiological risk factors and neurodegenerative disorders: An umbrella review of systematic reviews and meta-analyses.PDF
The role of microbiological factors in the development of neurodegenerative diseases is attracting increasing attention, while the relationship remains debated. This study aimed to comprehensively summarize and evaluate the associations between microbiological factors and the risk of neurodegenerative disorders with an umbrella review. PubMed, Embase, and the Cochrane library were used to search for papers from the earliest to March 2021 for identifying meta-analyses and systematic reviews that examined associations between microbiological factors and neurodegenerative diseases. AMSTAR2 tool was employed to evaluate the methodical quality of systematic reviews and meta-analyses. The effect size and 95% confidence interval (95% CI) were recalculated with a random effect model after the overlap was recognized by the corrected covered area (CCA) method. The heterogeneity of each meta-analysis was measured by the I2 statistic and 95% prediction interval (95% PI). Additionally, publication bias and the quality of evidence were evaluated for all 37 unique associations. Only 4 associations had above the medium level of evidence, and the rest associations presented a low level of evidence. Among them, helicobacter pylori (HP), infection, and bacteria are associated with Parkinson's disease (PD), and the other one verifies that periodontal disease is a risk factor for all types of dementia. Following the evidence of our study, eradication of HP and aggressive treatment of periodontitis are beneficial for the prevention of PD and dementia, respectively. This umbrella review provides comprehensive quality-grade evidence on the relationship between microbial factors and neurodegenerative disease. Regardless of much evidence linking microbial factors to neurodegenerative diseases, these associations are not necessarily causal, and the evidence level is generally low. Thus, more effective studies are required.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#searchadvanced, PROSPERO, identifier: CRD42021239512.</p