Labeling of human erythrocyte membrane proteins by photoactivatable radioiodinated phosphatidylcholine and phosphatidylserine A search for the aminophospholipid translocase

AbstractWe have synthesized radioiodinated photoactivatable phosphatidylcholine (125-N3-PC) and phosphatidylserine (125I-N3-PS). After incubation with red blood cells in the dark, the labeled PC could be extracted but not the corresponding PS molecule, indicating that the latter was transported by the aminophospholipid translocase, but not the former. When irradiated immediately after incorporation, N3-PS, but not N3-PC, partially blocked subsequent translocation of spin-labeled aminophospholipids. Analysis of probe distribution by SDSpolyacrylamide gel electrophoresis revealed that 125I-N3-PS labeled seven membrane bound components with molecular masses between 140 and 27 kDa: one (or several) of these components should correspond to the aminophospholipid translocase

Paramètres influençant la structure et la fonction du globule rouge chez le cheval

The erythrocyte, as an oxygen carrier, is submitted to areas where oxidative stress is important. The maintenance of the structure and fluidity of its membrane is essential to its function. Indeed, oxygen must diffuse through the membrane and the deformability of the cell is essential to its progression through capillaries. The structure and, as a consequence, the fluidity of the membrane influence these properties. The red blood cell presents antioxydant capacities, but in some cases, free radical production is increased and exceeds antiradical defences leading to irreversible defects of the membrane, and, as a consequence, of its function. Horse erythrocyte seems to be more sensitive to oxidative stress than other species and resulting hemorheological changes could have tissular and organic consequences.Le globule rouge, de par sa fonction de transport de l’oxygène, est soumis à des milieux où le stress oxydant est important. Le maintien de la structure et de la dynamique de sa membrane est essentiel à la réalisation de sa fonction. En effet, l’oxygène doit pouvoir diffuser à travers la membrane et la cellule doit être capable de se déformer pour progresser dans les capillaires. La structure et, en conséquence, la fluidité de la membrane influencent ces propriétés. Le globule rouge présente des défenses antioxydantes importantes, mais dans certaines situations la production de radicaux libres est accrue et dépasse ces défenses aboutissant à des lésions irréversibles de la membrane cellulaire et donc de la fonction. L’érythrocyte équin semble plus sensible au stress oxydant que celui des autres espèces et les modifications hemorhéologiques qui en résultent peuvent avoir des conséquences au niveau tissulaire et organique.Peer reviewe

Scenar 2030 - Pathways for the European agriculture and food sector beyond 2020 (Summary report)

Scenar 2030 aims at identifying major future trends and driving factors for European agriculture and rural regions and the perspectives and challenges resulting from them. The use of a suite of economic simulation models allows for the construction of a well-founded and plausible reference scenario (baseline) and different policy scenarios resulting in a comprehensive set of outcomes depicting economic, social and environmental indicators.JRC.D.4-Economics of Agricultur

A Comparative Analysis Shows Morphofunctional Differences between the Rat and Mouse Melanin-Concentrating Hormone Systems

Sub-populations of neurons producing melanin-concentrating hormone (MCH) are characterized by distinct projection patterns, birthdates and CART/NK3 expression in rat. Evidence for such sub-populations has not been reported in other species. However, given that genetically engineered mouse lines are now commonly used as experimental models, a better characterization of the anatomy and morphofunctionnal organization of MCH system in this species is then necessary. Combining multiple immunohistochemistry experiments with in situ hybridization, tract tracing or BrdU injections, evidence supporting the hypothesis that rat and mouse MCH systems are not identical was obtained: sub-populations of MCH neurons also exist in mouse, but their relative abundance is different. Furthermore, divergences in the distribution of MCH axons were observed, in particular in the ventromedial hypothalamus. These differences suggest that rat and mouse MCH neurons are differentially involved in anatomical networks that control feeding and the sleep/wake cycle

Navigating the evolving landscape of atopic dermatitis: Challenges and future opportunities: The 4th Davos declaration.

The 4th Davos Declaration was developed during the Global Allergy Forum in Davos which aimed to elevate the care of patients with atopic dermatitis (AD) by uniting experts and stakeholders. The forum addressed the high prevalence of AD, with a strategic focus on advancing research, treatment, and management to meet the evolving challenges in the field. This multidisciplinary forum brought together top leaders from research, clinical practice, policy, and patient advocacy to discuss the critical aspects of AD, including neuroimmunology, environmental factors, comorbidities, and breakthroughs in prevention, diagnosis, and treatment. The discussions were geared towards fostering a collaborative approach to integrate these advancements into practical, patient-centric care. The forum underlined the mounting burden of AD, attributing it to significant environmental and lifestyle changes. It acknowledged the progress in understanding AD and in developing targeted therapies but recognized a gap in translating these innovations into clinical practice. Emphasis was placed on the need for enhanced awareness, education, and stakeholder engagement to address this gap effectively and to consider environmental and lifestyle factors in a comprehensive disease management strategy. The 4th Davos Declaration marks a significant milestone in the journey to improve care for people with AD. By promoting a holistic approach that combines research, education, and clinical application, the Forum sets a roadmap for stakeholders to collaborate to improve patient outcomes in AD, reflecting a commitment to adapt and respond to the dynamic challenges of AD in a changing world

High-resolution 31 P- 1 H two-dimensional Nuclear Magnetic Resonance spectra of unsonicated lipid mixtures spinning at the magic-angle

International audienc

Morphofunctional Organization of the Connections From the Medial and Intermediate Parts of the Central Nucleus of the Amygdala Into Distinct Divisions of the Lateral Hypothalamic Area in the Rat

International audienc

The MCH neuron population as a model for the development and evolution of the lateral and dorsal hypothalamus

International audienc