Evaluation and comparison of the Amino acid composition of three varieties of Lagerena siceraria melon seed flours

Amino acids of three varieties of Lageneria siceraria seed flours were determined using standard methods of analysis. The three varieties are rich in essential amino acids needed for human health and growth, with a mean of 53.15%. Sulphur amino acid and aromatic amino acid              of the seeds ranged from 17.06 mg/g protein to 24.10 mg/g protein and from 80.22mg/g protein to 89.00 mg/g protein respectively. The varieties of these melon seed flours are closely related; however, there are significant differences (P?0.05) in Cystine, showing that the varieties have some genetic differences. Key Words: Lageneria siceraria seed flours, Essential amino acid, Genetics, Aginine,  Cystine

Sustainable Facilities Management Practices in Selected Universities in Ado-Odo/Ota Local Government Area, Ogun State, Nigeria

Sustainable development is being pursued at a global level and acknowledged across all sectors of the economy including universities. In order to apply sustainable measures, universities need to be vigilant in their management approaches. This study aimed at assessing the extent to which the selected universities in Ado-Oda/Ota Local Government Area of Ogun State have incorporated sustainability principles into their facility management practices. The study adopted a qualitative case study approach where interviews were conducted with experts from Covenant University (CU) and Bells University of Technology (BUT) who were purposefully sampled. The study population was comprised of facility managers from the physical planning and development units of the two universities who were chosen because of their expertise and responsibility. The interview guide contained questions about the types of buildings in the universities, sustainable facilities management (SFM) strategies being used by the universities, factors influencing the adoption of SFM strategies at the universities and challenges to the adoption of SFM practices. Data was analysed using conventional content approach. The study found that the universities have partially implemented SFM practices. The common practice adopted by the universities includes energy management. The universities have also done well in waste management. Legislation and cost savings were the drivers for the adoption of SFM practices. Despite such being the case, there are challenges to the adoption and implementation of SFM. These include resource constraints, design constraints, and lack of knowledge about SFM. Therefore, the study recommends development of a system that monitors and evaluates progress of sustainable facilities management practices in the Universities in Ado-Odo/Ota LGA, Ogun State

GENDER ANALYSIS: INFORMATION AND COMMUNICATION TECHNOLOGY (ICT) ADOPTION AMONG RURAL FARM DWELLERS IN OYO STATE, NIGERIA

The high illiteracy level in most rural communities of Nigeria is not representative of positive impacts that Information and Communication Technologies (ICTs) have had on Nigerian rural agriculture in terms of productivity, growth and development. The study investigated the adoption of ICTs among rural farm dwellers in Iseyin Local Government Area of Oyo state, Nigeria, using gender analysis. A well-structured interview schedule was employed to elicit quantitative information from 60 male and 60 female rural farm dwellers from Serafu, Ado-awaye and Osoogun in Iseyin Local Government Areas, using the two stage random sampling procedure. Results showed that 55% of the male respondents and 46.7% of the female respondents adopted various selected ICTs in general. Analysis of Variance (ANOVA) at <0.05 level of significance resulted that a significant difference exists in the adoption of the ICTs of both male and female respondents (F= 4.198). The findings revealed that at <0.05 level of significance, significant difference existed in the level of income of the male and female respondents (F= 4.079). The study revealed that male farmers adopted ICTs more than their female counterparts, and there were significant gender gaps in ICT adoption among rural farm dwellers in Nigeria. Therefore, in order to bridge the gender gap in the level of adoption of ICTs, among rural dwellers, it was recommended that various ICTs should be made available for rural dwellers at subsidized rate because larger percentage of them are aware of the importance and benefits of ICTs but lack the financial capacity to acquire them. The government should formulate policy that will enhance continuous development, dissemination, and utilization of gender responsive ICTs for farming activities and for development of rural livelihood, in Nigeria

Phytochemical screening and proximate analysis of young Cola acuminata leaves

Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. Phytochemical screening and proximate analysis of dried young cola nitida leaves used in folklore medicine were carried out. The result revealed the presence of bioactive components comprising of alkaloids, phenolic acid, carotenoids, and flavonoid. Quantitative phytochemical analyses showed that alkaloid have the highest concentration. Proximate analysis of the sample showed the moisture content of the sample is (23.10±0.12), carbohydrate (36.80±0.25), crude fibre (19.20±0.1), crude protein (13.50±0.10), and ash content (6.70±0.07). The presence of secondary metabolites in this plant is indicative that if well researched, novel bioactive compounds can be discovered in it

Measurement and Comparison of Total Electron Content for Assessment of Ionospheric Models during April 7, 2000 Geomagnetic Storms

Ionospheric modelling is a major approach to predicting the behavior of the ionosphere particularly in regions where Global Positioning Systems (GPS) are not readily available. Hence, the objective of this paper is to measure and compare Total Electron Content (TEC) for Assessment of Ionospheric Models during April 7, 2000 Geomagnetic Storms. Measured Total Electron Content (TEC) from experimental records (April 5 - 9, 2000) were compared with those predicted by the improved versions of the International Reference Ionosphere (IRI-2012 and IRI-Plas2015) and the NeQuick models. The mean values of TEC in five days of the months were plotted against the hours of the same day and the root mean square error of the models which shows their deviations from the GPS data were used to observe the diurnal variations in TEC and the performances of the ionospheric models respectively. The data obtained confirmed that TEC has their highest values during the midnight period and lowest values during the sunset period at the Australian stations and we also confirmed that European stations had their highest TEC values during the daytime and their lowest values during the night time. We affirmed that the North American station in USA had its highest TEC values during the night time and lowest values during day time. The Asian station had its highest TEC values during the day time and lowest values during the midnight period. However, NeQuick, IRIPlas2015, and NeQ-IRI produced better estimate of TEC than the IRI-2001 and IRI-2001COR at all locations during the phases of the geomagnetic storm

Novel functional insights into ischemic stroke biology provided by the first genome-wide association study of stroke in indigenous Africans

\ua9 The Author(s) 2024. Background: African ancestry populations have the highest burden of stroke worldwide, yet the genetic basis of stroke in these populations is obscure. The Stroke Investigative Research and Educational Network (SIREN) is a multicenter study involving 16 sites in West Africa. We conducted the first-ever genome-wide association study (GWAS) of stroke in indigenous Africans. Methods: Cases were consecutively recruited consenting adults (aged &gt; 18 years) with neuroimaging-confirmed ischemic stroke. Stroke-free controls were ascertained using a locally validated Questionnaire for Verifying Stroke-Free Status. DNA genotyping with the H3Africa array was performed, and following initial quality control, GWAS datasets were imputed into the NIH Trans-Omics for Precision Medicine (TOPMed) release2 from BioData Catalyst. Furthermore, we performed fine-mapping, trans-ethnic meta-analysis, and in silico functional characterization to identify likely causal variants with a functional interpretation. Results: We observed genome-wide significant (P-value &lt; 5.0E−8) SNPs associations near AADACL2 and miRNA (MIR5186) genes in chromosome 3 after adjusting for hypertension, diabetes, dyslipidemia, and cardiac status in the base model as covariates. SNPs near the miRNA (MIR4458) gene in chromosome 5 were also associated with stroke (P-value &lt; 1.0E−6). The putative genes near AADACL2, MIR5186, and MIR4458 genes were protective and novel. SNPs associations with stroke in chromosome 2 were more than 77 kb from the closest gene LINC01854 and SNPs in chromosome 7 were more than 116 kb to the closest gene LINC01446 (P-value &lt; 1.0E−6). In addition, we observed SNPs in genes STXBP5-AS1 (chromosome 6), GALTN9 (chromosome 12), FANCA (chromosome 16), and DLGAP1 (chromosome 18) (P-value &lt; 1.0E−6). Both genomic regions near genes AADACL2 and MIR4458 remained significant following fine mapping. Conclusions: Our findings identify potential roles of regulatory miRNA, intergenic non-coding DNA, and intronic non-coding RNA in the biology of ischemic stroke. These findings reveal new molecular targets that promise to help close the current gaps in accurate African ancestry-based genetic stroke’s risk prediction and development of new targeted interventions to prevent or treat stroke

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke — the second leading cause of death worldwide — were conducted predominantly in populations of European ancestry1,2. Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis3, and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach4, we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry5. Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke - the second leading cause of death worldwide - were conducted predominantly in populations of European ancestry(1,2). Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis(3), and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach(4), we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry(5). Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries.</p

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke — the second leading cause of death worldwide — were conducted predominantly in populations of European ancestry1,2. Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis3, and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach4, we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry5. Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries

Fakunle,et al,Paste Production Paste Production From Synodontis Membranaceus Using Different Percentages of Ginger (Zingiber officinale)

Abstract: Synodontis membranaceus was subjected to fermentation (34 +-3 o C) for a period of 4-weeks (one month) with varying concentrations of ginger (5%, 10%, 15%, 20%) and 20% salt as spices to produce paste. The samples were analyzed for proximate composition, pH, microbial load and organoleptic properties at the beginning and end of the fermentation. Results showed steady increase in nutrients such as crude fat, ash, and NFE (nitrogen free extract) but decrease in crude fibre. There were fluctuations in the crude protein of the fermented fish samples. There was an increase in the microbial load of the fermented fish and a slight decrease in the moisture content. The organoleptic test showed preference for taste, aroma, and overall acceptability for fish fermented with 20% ginger