Selenium Biotransformations in an Engineered Aquatic Ecosystem for Bioremediation of Agricultural Wastewater via Brine Shrimp Production

An engineered aquatic ecosystem was specifically designed to bioremediate selenium (Se), occurring as oxidized inorganic selenate from hypersalinized agricultural drainage water while producing brine shrimp enriched in organic Se and omega-3 and omega-6 fatty acids for use in value added nutraceutical food supplements. Selenate was successfully bioremediated by microalgal metabolism into organic Se (seleno-amino acids) and partially removed via gaseous volatile Se formation. Furthermore, filterfeeding brine shrimp that accumulated this organic Se were removed by net harvest. Thriving in this engineered pond system, brine shrimp (Artemia franciscana Kellogg) and brine fly (Ephydridae sp.) have major ecological relevance as important food sources for large populations of waterfowl, breeding, and migratory shore birds. This aquatic ecosystem was an ideal model for study because it mimics trophic interactions in a Se polluted wetland. Inorganic selenate in drainage water was metabolized differently in microalgae, bacteria, and diatoms where it was accumulated and reduced into various inorganic forms (selenite, selenide, or elemental Se) or partially incorporated into organic Se mainly as selenomethionine. Brine shrimp and brine fly larva then bioaccumulated Se from ingesting aquatic microorganisms and further metabolized Se predominately into organic Se forms. Importantly, adult brine flies, which hatched from aquatic larva, bioaccumulated the highest Se concentrations of all organisms tested

Formation of Zn–Ca phyllomanganate nanoparticles in grass roots

International audienceIt is now well established that a number of terrestrial and aquatic microorganisms have the capacity to oxidize and precipitate Mn as phyllomanganate. However, this biomineralization has never been shown to occur in plant tissues, nor has the structure of a natural Mn(IV) biooxide been characterized in detail. We show that the graminaceous plant Festuca rubra (red fescue) produces a Zn-rich phyllomanganate with constant Zn:Mn and Ca:Mn atomic ratios (0.46 and 0.38, respectively) when grown on a contaminated sediment. This new phase is so far the Zn-richest manganate known to form in nature (chalcophanite has a Zn:Mn ratio of 0.33) and has no synthetic equivalent. Visual examination of root fragments under a microscope shows black precipitates about ten to several tens of microns in size, and their imaging with backscattered and secondary electrons demonstrates that they are located in the root epidermis. In situ measurements by Mn and Zn K-edge extended X-ray absorption fine structure (EXAFS) spectroscopy and X-ray diffraction (XRD) with a micro-focused beam can be quantitatively described by a single-phase model consisting of Mn(IV) octahedral layers with 22% vacant sites capped with tetrahedral and octahedral Zn in proportions of 3:1. The layer charge deficit is also partly balanced by interlayer Mn and Ca. Diffracting crystallites have a domain radius of 33 Å in the ab plane and contain only 1.2 layers (not, vert, similar8.6 Å) on average. Since this biogenic Mn oxide consists mostly of isolated layers, basal 00l reflections are essentially absent despite its lamellar structure. Individual Mn layers are probably held together in the Mn–Zn precipitates by stabilizing organic molecules. Zinc biomineralization by plants likely is a defense mechanism against toxicity induced by excess concentrations of this metal in the rhizosphere

Evolution of CODYRUN from Thermal Simulation to Coupled Thermal and Daylight Simulation Software

AbstractCODYRUN is a multi-zone software integrating thermal building simulation, airflow, and pollutant transfer. Described in numerous publications, this software was originally used for the passive design of buildings, both for research and teaching purposes. In this context, the data treated were mainly concerned with volumes (zones), surfaces and thicknesses (walls and windows), materials, and systems, with the aim to determine temperatures, heat fluxes, energy consumed, air transfers, and so on.The question thus arose as to the integration of indoor lighting conditions into the simulation. Hence, previous data structures had to be amended to incorporate the spatial positioning of entities (walls, windows, and artificial lighting sources) through vertexes. A set of procedures was also developed for polygons as well as calculating natural and artificial lighting.The results of this new daylighting module were then compared with other results of simulation codes and experimental cases both in artificial and natural environments. Excellent agreements were obtained, such as the values for luminous efficiencies in a tropical and humid climate.A simulation exercise was conducted in a classroom located in Reunion Island (French overseas territory in the Indian Ocean), thus confirming the interest for thermal and daylighting designs in low-energy buildings

Development of a Displacement Sensor for the CERN-LHC Superconducting Cryodipoles

One of the main challenges of the Large Hadron Collider (LHC), the particle accelerator under construction at CERN (the European Organization for Nuclear Research) in Geneva, resides in the design and production of the superconducting dipoles used to steer the particles around a 27 km underground tunnel. These so-called cryodipoles are composed of an evacuated cryostat and a cold mass, that contains the particle tubes and the superconducting dipole magnet and is cooled by super uid Helium at 1.9 K. The particle beam must be centred within the dipole magnetic field with a sub-millimetre accuracy, this requires in turn that the relative displacements between the cryostat and the cold mass must be monitored with accuracy. Because of the extreme environmental conditions (the displacement measurements must be made in vacuum and between two points at a temperature difference of about 300 degrees) no adequate existing monitoring system was found for this application. It was therefore decided to develop an optical sensor suitable for this application. This contribution describes the development of this novel sensor and the first measurements performed on the LHC cryodipoles

Heat Transfer in Buildings: Application to Solar Air Collector and Trombe Wall Design

The aim of this paper is to briefly recall heat transfer modes and explain their integration within a software dedicated to building simulation (CODYRUN). Detailed elements of the validation of this software are presented and two applications are finally discussed. One concerns the modeling of a flat plate air collector and the second focuses on the modeling of Trombe solar walls. In each case, detailed modeling of heat transfer allows precise understanding of thermal and energetic behavior of the studied structures. Recent decades have seen a proliferation of tools for building thermal simulation. These applications cover a wide spectrum from very simplified steady state models to dynamic simulation ones, including computational fluid dynamics modules (Clarke, 2001). These tools are widely available in design offices and engineering firms. They are often used for the design of HVAC systems and still subject to detailed research, particularly with respect to the integration of new fields (specific insulation materials, lighting, pollutants transport, etc.). Available from: http://www.intechopen.com/books/evaporation-condensation-and-heat-transfer/heat-transfer-in-buildings-application-to-solar-air-collector-and-trombe-wall-designComment: Available from: http://www.intechopen.com/books/evaporation-condensation-and-heat-transfer/heat-transfer-in-buildings-application-to-solar-air-collector-and-trombe-wall-desig

Iron Speciation in PM2.5 from Urban, Agriculture, and Mixed Environments in Colorado, USA

Atmospheric iron solubility varies depending on whether the particles are collected in rural or urban areas, with urban areas showing increased iron solubility. In this study, we investigate if the iron species present in different environments affects its ultimate solubility. Field data are presented from the Platte River Air Pollution and Photochemistry Experiment (PRAPPE), aimed at understanding the interactions between organic carbon and trace elements in atmospheric particulate matter (PM). 24-hr PM2.5 samples were collected during the summer and winter (2016–2017), at three different sites on the Eastern Colorado plains: an urban, agricultural, and a mixed site. Downtown Denver had an average total and water-soluble iron air concentration of 181.2 and 7.7 ng m−3, respectively. Platteville, the mixed site, had an average of total iron of 76.1 ng m−3, with average water-soluble iron concentration of 9.1 ng m−3. Jackson State Park (rural/agricultural) had the lowest total iron average of 31.5 ng m−3 and the lowest water-soluble iron average, 1.3 ng m−3. The iron oxidation state and chemical speciation of 97 samples across all sites and seasons was probed by X-ray absorption near edge structure (XANES) spectroscopy. The most common iron phases observed were almandine (Fe₃Al₂Si₃O₁₂) (Denver 21%, Platteville 16%, Jackson 24%), magnetite (Fe3O4) (Denver 9%, Platteville 4%, Jackson 5%) and Fe (III)dextran (Denver 5%, Platteville 13%, Jackson 5%), a surrogate for Fe-organic complexes. Additionally, native iron [Fe(0)] was found in significant amounts at all sites. No correlation was observed between iron solubility and iron oxidation state or chemical speciation

Zinc distribution and speciation in Arabidopsis halleri x Arabidopsis lyrata progenies presenting various zinc accumulation capacities

The definitive version is available at http://www.blackwell-synergy.com/loi/nphInternational audienceThe purpose of this study was to investigate the relationship between the chemical form and localization of zinc (Zn) in plant leaves and their Zn accumulation capacity. * An interspecific cross between Arabidopsis halleri sp. halleri and Arabidopsis lyrata sp. petrea segregating for Zn accumulation was used. Zinc (Zn) speciation and Zn distribution in the leaves of the parent plants and of selected F1 and F2 progenies were investigated by spectroscopic and microscopic techniques and chemical analyses. * A correlation was observed between the proportion of Zn being in octahedral coordination complexed to organic acids and free in solution (Zn-OAs + Znaq) and Zn content in the leaves. This pool varied between 40% and 80% of total leaf Zn depending on the plant studied. Elemental mapping of the leaves revealed different Zn partitioning between the veins and the leaf tissue. The vein : tissue fluorescence ratio was negatively correlated with Zn accumulation. * The higher proportion of Zn-OAs + Znaq and the depletion of the veins in the stronger accumulators are attributed to a higher xylem unloading and vacuolar sequestration in the leaf cells. Elemental distributions in the trichomes were also investigated, and results support the role of carboxyl and ⁄ or hydroxyl groups as major Zn ligands in these cells