56 research outputs found

    Characterization of dedifferentiating human mature adipocytes from the 6 visceral and subcutaneous fat compartments : fibroblast-activation protein 7 alpha and Dipeptidyl peptidase 4 as major components of matrix remodeling

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    Mature adipocytes can reverse their phenotype to become fibroblast-like cells. This is achieved by ceiling culture and the resulting cells, called dedifferentiated fat (DFAT) cells, are multipotent. Beyond the potential value of these cells for regenerative medicine, the dedifferentiation process itself raises many questions about cellular plasticity and the pathways implicated in cell behavior. This work has been performed with the objective of obtaining new information on adipocyte dedifferentiation, especially pertaining to new targets that may be involved in cellular fate changes. To do so, omental and subcutaneous mature adipocytes sampled from severely obese subjects have been dedifferentiated by ceiling culture. An experimental design with various time points along the dedifferentiation process has been utilized to better understand this process. Cell size, gene and protein expression as well as cytokine secretion were investigated. Il-6, IL-8, SerpinE1 and VEGF secretion were increased during dedifferentiation, whereas MIF-1 secretion was transiently increased. A marked decrease in expression of mature adipocyte transcripts (PPARγ2, C/EBPα, LPL and Adiponectin) was detected early in the process. In addition, some matrix remodeling transcripts (FAP, DPP4, MMP1 and TGFβ1) were rapidly and strongly up-regulated. FAP and DPP4 proteins were simultaneously induced in dedifferentiating mature adipocytes supporting a potential role for these enzymes in adipose tissue remodeling and cell plasticity

    Assortiment : comment passer d'une logique nationale à une offre locale ?

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    Les problématiques liées aux assortiments se veulent nombreuses et complexes. Nous allons centrer l'analyse sur une question singulière et encore peu traitée dans la littérature: comment les enseignes passent-elles d'une démarche nationale à une offre locale, contextualisée ? Nous commencerons tout d'abord par quelques rappels sur la démarche de construction des assortiments. Puis, après avoir décrit comment les grands distributeurs passent d'un marketing national à une déclinaison locale des assortiments, nous aborderons une étude de cas réelle, celle des magasins Champio

    Management de la distribution

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    Cet ouvrage offre une synthèse sur le management de la distribution et analyse à la fois les différentes formes de canaux, les stratégies spécifiques des entreprises de distribution et les problématiques propres au management de ce secteur très important de l'économi
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