A Human-Relevant 3D In Vitro Platform for an Effective and Rapid Simulation of Workplace Exposure to Nanoparticles: Silica Nanoparticles as Case Study

In this contribution, we show the suitability of a 3D airway model, when coupled with a nebulizer system, for simulating workplace exposure to nanoparticles. As a proof of concept, workplace exposure to silica nanoparticles was experimentally measured in an occupational facility where nanoparticles are produced weekly, and compared with the official limit value for bulk silica materials. These values of potential exposure were simulated in a 3D airway model by nebulizing low doses (from 0.90 to 55 µg/cm2) of silica nanoparticles over a prolonged period (12 weeks of repeated exposure, 5 days per week). Overall, the results suggest the efficiency of the defense mechanisms of the respiratory system and the clearance of the breathed silica nanoparticles by the mucociliary apparatus in accordance with the recent in vivo data. This in vitro platform shows that the doses tested may correlate with the occupational exposure limit values. Such relationship could provide regulatory-oriented data useful for risk classification of nanomaterials

Contribution of ICOH to Address the Different OSH Needs among Countries: Results of a Survey

The aim of this study is to map the coverage of occupational safety and health (OSH) rules and provisions and their enforcement at a country level worldwide. Members’ participation in the International Commission on Occupational Health (ICOH) activities was also investigated. We used a questionnaire-based survey to collect data. An online questionnaire was administered from February 14 to March 18, 2018 to all ICOH members for the triennium 2015 to 2017 (n = 1929). We received 384 completed questionnaires from 79 countries, with a 20% response rate. To synthesize information about the coverage of OSH rules and provisions and their level of enforcement, a synthetic coverage index was calculated and combined with country, gross domestic product (GDP) per capita and the human development index (HDI). We used multiple correspondence analysis (MCA) to analyze the members’ participation in ICOH activities. More than 90.0% of the sample declared that in their own country there is a set of rules and provisions regulating OSH in the workplace, and training procedures and tools to improve workers’ awareness. However, these rules and training procedures are mainly “partially” enforced and utilized (39.0% and 45.4%). There was no statistically significant association between country and GDP per capita and the synthetic coverage index, whilst controlling for HDI. The level of engagement in ICOH activities is higher in senior members (aged 65 years or older), coming from high-income countries, having held a position within ICOH, with a higher level of education and a researcher position. An integrated and multidisciplinary approach, which includes research, education and training, is needed to address OSH issues and their impact both at global and country level

Nanomateriali e salute sul lavoro. Il contributo dell'INAIL alla ricerca per l'innovazione responsabile e la tutela dei lavoratori in Italia

Le nanotecnologie sono in costante evoluzione e grazie alle loro enormi potenzialità i prodotti che le utilizzano sono in continuo aumento. Sono sempre più frequentemente impiegate nella nostra vita quotidiana, trovando applicazione in molteplici settori come quelli rappresentati dal mercato tessile, automobilistico, elettronico, alimentare, ecc. Nel 2012 la Commissione Europea ha incluso le nanotecnologie nelle cinque Key Enabling Technologies (KETs), che forniscono la base per l’innovazione e la creazione di nuovi prodotti. Il crescente interesse industriale e produttivo ha portato inevitabilmente ad un incremento del numero di lavoratori esposti, nelle fasi di sintesi e produzione fino alle fasi di smaltimento o recupero del rifiuto. Contemporaneamente alla crescita della produzione globale stimata in circa 11 milioni di tonnellate annue, sono cresciute anche le preoccupazioni riguardo i possibili effetti negativi sulla salute umana, focalizzando l’attenzione della comunità scientifica, in particolare sul tema della salute e sicurezza negli ambienti di lavoro. Anche in Italia il mercato industriale si è evoluto nella stessa direzione con importanti investimenti economici sia pubblici che privati ed un conseguente coinvolgimento di numerosi gruppi di ricerca afferenti ad Università, Istituti ed Enti di Ricerca nello studio delle principali tematiche relative allo sviluppo responsabile dei nanomateriali. In tale contesto, il presente studio intende evidenziare l’impegno della ricerca Italiana nell’ambito della salute e sicurezza dei nanomateriali ingegnerizzati, attraverso il contributo e le iniziative dell’INAIL per promuovere lo sviluppo sostenibile delle nanotecnologie

Occupational Exposure to Silica Nanoparticles: Evaluation of Emission Fingerprints by Laboratory Simulations

Silica nanoparticles (SiO2 NPs), due to their chemical-physical properties, are among the most widely produced nanomaterials (NMs) in the world, and therefore used in a wide range of industries. Such widespread use, however, draws attention to the health of workers during the production of such NMs and the need for techniques to assess occupational exposure. In the present study, laboratory simulation techniques were used to reproduce a critical work activity in a controlled environment in order to identify emission profiles useful for studying exposure during NM handling in the workplace. Weighing activity inside a glove box isolated from the external environment background and any pollutants was simulated. Real-time instrumentation was used to calculate the concentration, size distribution and surface area of the particles generated during the simulation, and time-integrated instrumentation was used to collect dust for off-line analysis

Risk assessment at work and prevention strategies on COVID-19 in Italy.

The COVID-19 pandemic has spread worldwide, with considerable public health and socio-economic impacts that are seriously affecting health and safety of workers, as well as their employment stability. Italy was the first of many other western countries to implement extended containment measures. Health workers and others employed in essential sectors have continued their activity, reporting high infection rate with many fatalities. The epidemiological trend highlighted the importance of work as a substantial factor to consider both when implementing strategies aimed at containing the pandemic and shaping the lockdown mitigation strategy required for sustained economic recovery. To support the decision-making process, we have developed a strategy to predict the risk of infection by SARS-CoV-2 in the workplace based on the analysis of the working process and proximity between employees; risk of infection connected to the type of activity; involvement of third parties in the working processes and risk of social aggregation. We applied this approach to outline a risk index for each economic activity sector, with different levels of detail, also considering the impact on mobility of the working population. This method was implemented into the national epidemiological surveillance model in order to estimate the impact of re-activation of specific activities on the reproduction number. It has also been adopted by the national scientific committee set up by the Italian Government for action-oriented policy advice on the COVID-19 emergency in the post lockdown phase. This approach may play a key role for public health if associated with measures for risk mitigation in enterprises through strategies of business process re-engineering. Furthermore, it will make a contribution to reconsidering the organization of work, including also innovation and fostering the integration with the national occupational safety and health (OSH) system

Workers’ Exposure Assessment during the Production of Graphene Nanoplatelets in R&D Laboratory

Widespread production and use of engineered nanomaterials in industrial and research settings raise concerns about their health impact in the workplace. In the last years, graphene-based nanomaterials have gained particular interest in many application fields. Among them, graphene nanoplatelets (GNPs) showed superior electrical, optical and thermal properties, low-cost and availability. Few and conflicting results have been reported about toxicity and potential effects on workers’ health, during the production and handling of these nanostructures. Due to this lack of knowledge, systematic approaches are needed to assess risks and quantify workers’ exposure to GNPs. This work applies a multi-metric approach to assess workers’ exposure during the production of GNPs, based on the Organization for Economic Cooperation and Development (OECD) methodology by integrating real-time measurements and personal sampling. In particular, we analyzed the particle number concentration, the average diameter and the lung deposited surface area of airborne nanoparticles during the production process conducted by thermal exfoliation in two different ways, compared to the background. These results have been integrated by electron microscopic and spectroscopic analysis on the filters sampled by personal impactors. The study identifies the process phases potentially at risk for workers and reports quantitative information about the parameters that may influence the exposure in order to propose recommendations for a safer design of GNPs production process