Ultrafast Dynamics of Metal Complexes of Tetrasulfonated Phthalocyanines at Biological Interfaces: Comparison between Photochemistry in Solutions, Films, and Noncancerous and Cancerous Human Breast Tissues

International audienceA promising material in medicine, electronics, opto-electronics, electrochemistry, catalysis, and photophysics, Al(III) phthalocyanine chloride tetrasulfonic acid (AlPcS4) is investigated at biological interfaces of human breast tissue by means of steady-state and time-resolved pump?probe spectroscopies: IR, Raman, UV?vis, fluorescence, and electronic transient absorption by pump?probe spectroscopy. Spectrally resolved pump?probe data were recorded on time scales ranging from femtoseconds to nanoseconds and give insight into molecular interactions and primary events in the interfacial region. The nature of these fast processes and pathways of the competing relaxation processes from the initially excited electronic states in AlPcS4 films and at biological interfaces of human breast cancerous and noncancerous tissues is studied. Comparison between photochemical dynamics in the biological environment of the human breast tissues and that occurring in aqueous solutions is presented. The excited-state absorption (ESA) decays and bleaching recovery of the ground state have been fitted in the time window extending to nanoseconds (0?1 ns). We found that the excited-state dynamics of AlPcS4 at biological interfaces of human breast tissue is extremely sensitive to the biological environment and differs drastically from that observed in solutions and films. We demonstrated that the ultrafast dynamics at biological interfaces is described by three time constants in the ranges of 110?170 fs, 1?7 ps, and 20?60 ps. We were able to ascribe these three time constants to the primary events occurring in phthalocyanine at biological interfaces. The shortest time constants have been assigned to vibrational wavepacket dynamics in the Franck?Condon region down to the local minimum of the excited-state S1. The 1?7 ps components have been assigned to vibrational relaxation in the excited and ground electronic states. In contrast to the dynamics observed in aqueous solutions with the components in the range of 150?500 ps assigned to decay from S1 to the ground electronic state, these slow components have not been recorded in human breast tissue. We have shown that the lifetimes characterizing the first excited-state S1 in the interfacial regions of the breast tissue are markedly shorter than those in solution. It suggests that molecular structures responsible for harvesting of the light energy in biological tissue find their own ways for recovery through some special features of the potential energy surfaces such as conical intersections, which facilitate the rate of radiationless transitions. We found that the dynamics of photosensitizers in normal (noncancerous) breast tissue is markedly faster than that in cancerous tissue

Ultrafast Dynamics of Metal Complexes of Tetrasulphonated Phthalocyanines

International audienceA promising material in medicine, electronics, optoelectronics, electrochemistry, catalysis, and photophysics, tetrasulphonated aluminum phthalocyanine (AlPcS4), is investigated by means of steady-state and time-resolved pump?probe spectroscopies. Absorption and steady-state fluorescence spectroscopy indicate that AlPcS4 is essentially monomeric. Spectrally resolved pump-probe data are recorded on time scales ranging from femtoseconds to nanoseconds. The nature of these fast processes and pathways of the competing relaxation processes from the initially excited electronic states in aqueous and organic (dimethyl sulfoxide) solutions are discussed. The decays and bleaching recovery have been fitted in the ultrafast window (0-10 ps) and later time window extending to nanoseconds (0-1 ns). While the excited-state dynamics have been found to be sensitive to the solvent environment, we were able to show that the fast dynamics is described by three time constants in the ranges of 115-500 fs, 2-25 ps, and 150-500 ps. We were able to ascribe these three time constants to different processes. The shortest time constants have been assigned to vibrational wavepacket dynamics. The few picosecond components have been assigned to vibrational relaxation in the excited electronic states. Finally, the 150-500 ps components represent the decay from S1 to the ground state. The experimental and theoretical treatment proposed in this paper provides a basis for a substantial revision of the commonly accepted interpretation of the Soret transition (B transition) that exists in the literature

Impact of whole-body computed tomography on mortality and surgical management of severe blunt trauma

International audienceIntroductionThe mortality benefit of whole-body computed tomography (CT) in early trauma management remains controversial and poorly understood. The objective of this study was to assess the impact of whole-body CT compared with selective CT on mortality and management of patients with severe blunt trauma.MethodsThe FIRST (French Intensive care Recorded in Severe Trauma) study is a multicenter cohort study on consecutive patients with severe blunt trauma requiring admission to intensive care units from university hospital trauma centers within the first 72 hours. Initial data were combined to construct a propensity score to receive whole-body CT and selective CT used in multivariable logistic regression models, and to calculate the probability of survival according to the Trauma and Injury Severity Score (TRISS) for 1,950 patients. The main endpoint was 30-day mortality.ResultsIn total, 1,696 patients out of 1,950 (87%) were given whole-body CT. The crude 30-day mortality rates were 16% among whole-body CT patients and 22% among selective CT patients (p = 0.02). A significant reduction in the mortality risk was observed among whole-body CT patients whatever the adjustment method (OR = 0.58, 95% CI: 0.34-0.99 after adjustment for baseline characteristics and post-CT treatment). Compared to the TRISS predicted survival, survival significantly improved for whole-body CT patients but not for selective CT patients. The pattern of early surgical and medical procedures significantly differed between the two groups.ConclusionsDiagnostic whole-body CT was associated with a significant reduction in 30-day mortality among patients with severe blunt trauma. Its use may be a global indicator of better management

Impact of emergency medical helicopter transport directly to a university hospital trauma center on mortality of severe blunt trauma patients until discharge

International audienceIntroductionThe benefits of transporting severely injured patients by helicopter remain controversial. This study aimed to analyze the impact on mortality of helicopter compared to ground transport directly from the scene to a University hospital trauma center.MethodsThe French Intensive Care Research for Severe Trauma cohort study enrolled 2,703 patients with severe blunt trauma requiring admission to University hospital intensive care units within 72 hours. Pre-hospital and hospital clinical data, including the mode of transport, (helicopter (HMICU) versus ground (GMICU), both with medical teams), were recorded. The analysis was restricted to patients admitted directly from the scene to a University hospital trauma center. The main endpoint was mortality until ICU discharge.ResultsOf the 1,958 patients analyzed, 74% were transported by GMICU, 26% by HMICU. Median injury severity score (ISS) was 26 (interquartile range (IQR) 19 to 34) for HMICU patients and 25 (IQR 18 to 34) for GMICU patients. Compared to GMICU, HMICU patients had a higher median time frame before hospital admission and were more intensively treated in the pre-hospital phase. Crude mortality until hospital discharge was the same regardless of pre-hospital mode of transport. After adjustment for initial status, the risk of death was significantly lower (odds ratio (OR): 0.68, 95% confidence interval (CI) 0.47 to 0.98, P = 0.035) for HMICU compared with GMICU. This result did not change after further adjustment for ISS and overall surgical procedures.ConclusionsThis study suggests a beneficial impact of helicopter transport on mortality in severe blunt trauma. Whether this association could be due to better management in the pre-hospital phase needs to be more thoroughly assessed

Evaluation hémodynamique comparative entre le sytème de compression active (Lucas®) et le massage manuel chez les patients victimes d'un arrêt cardiaque extra-hospitalier

Résumé françaisDIJON-BU Médecine Pharmacie (212312103) / SudocSudocFranceF

Apport de la mesure temps réel de l'activité du système nerveux autonome dans l'aide à la détection de la mort encéphalique (résultats préliminaires)

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Etude de l'action antioxydante des anesthésiques locaux sur un modèle de globules rouges soumis à une agression radicalaire

@LORSQUE DES GLOBULES ROUGES SONT SOUMIS A UNE AGRESSION RADICALAIRE, ON OBSERVE UNE OXYDATION DES LIPIDES ET PROTEINES MEMBRANAIRES RESPONSABLE D'UNE FUITE POTASSIQUE ET D'UNE HEMOLYSE. LE BUT DU PRESENT TRAVAIL ETAIT D'ETUDIER LES EFFETS ANTIOXYDANTS DES ANESTHESIQUES LOCAUX SUR DES GLOBULES ROUGES HUMAINS SOUMIS A UNE AGRESSION RADICALAIRE. DANS LES CONDITIONS EXPERIMENTALES, LA LIDOCAINE DIMINUAIT L'EFFET PO ASSIQUE ET L'HEMOLYSE, MAIS ETAIT DENUEE D'EFFET PIEGEUR DE RADICAUX LIBRES. L'ACTION DE L'OUBAINE, DU LEMAKALIM ET DU FUROSEMIDE A ETE EGALEMENT TESTEE. L'OUBAINE ET LE LEMAKALIM ETAIENT SANS EFFET, CE QUI ELIMINAIT UNE ACTION IMPLIQUANT UNE INHIBITION DE LA Na-K ATPase OU UNE OUVERTURE DES CANAUX Katp. LE FUROSEMIDE SE COMPORTANT COMME UN PIEGEUR DE RADICAUX LIBRES, IL N'A PAS ETE POSSIBLE DE PRECISER LE ROLE DE L'ECHANGEUR Na-K-Cl. LA COMPARAISON DES EFFETS DE LA LIDOCAINE AVEC CEUX DE LA BUPIVACAINE ET DE LA ROPIVACAINE A MONTRE QUE LA LIDOCAINE ETAIT PLUS EFFICACE QUE LES DEUX AUTRES MOLECULES, CE QUI ECARTAIT L'HYPOTHESE D'UN MECANISME IMPLIQUANT LE CANAL SODIQUE ET L'EVENTUEL INFLUENCE DE LA STEREOISOMERIE. SECONDAIREMENT, CE MODELE D'ETUDE A ETE UTILISE POUR VERIFIER IN VITRO L'INTERACTION ENTRE LE FLUMAZENIL ET LA BUPIVACAINE. CETTE INTERACTION A ETE RETROUVEE , CONFIRMANT AINSI QU'ELLE SIEGEAIT AU NIVEAU DE LA MEMBRANE CELLULAIRE.ENFIN, IL A ETE MONTRE QUE LA LIDOCAINE DIMINUAIT L'EFFLUX POTASSIQUE ET L'HEMOLYSE LORSQUE DES GLOBULES ROUGES ETAIENT CONSERVES PENDANT UNE SEMAINE. TOUS CES RESULTATS CONFIRMENT LA VALIDITE DE CE MODELE POUR L'ETUDE DES EFFETS DE MOLECULES SUR LA MEMBRANE CELLULAIRE.LA LODOCAINE POSSEDE UN PUISSANT EFFET PROTECTEUR , IMPLIQUANT VRAISEMBLABLEMENT UNE ACTION DIRECTE AU SEIN DE LA MEMBRANE CELLULAIRE.DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Evaluation de la régulation libérale des appels de permanence de soins en Côte d'Or par une triple enquête prospective de satisfaction

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

DEPISTAGE DES LESIONS CERVICALES CHEZ LE PATIENT TRAUMATISE INCONSCIENT (INTERET DE LA TOMODENSITOMETRIE ET DES CLICHES STANDARDS)

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF