564 research outputs found
A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding
In bacterial c-type cytochromes, the haem cofactor is covalently attached via two cysteine residues organized in a haem c-binding motif. Here, a novel octa-haem c protein, MccA, is described that contains only seven conventional haem c-binding motifs (CXXCH), in addition to several single cysteine residues and a conserved CH signature. Mass spectrometric analysis of purified MccA from Wolinella succinogenes suggests that two of the single cysteine residues are actually part of an unprecedented CX15CH sequence involved in haem c binding. Spectroscopic characterization of MccA identified an unusual high-potential haem c with a red-shifted absorption maximum, not unlike that of certain eukaryotic cytochromes c that exceptionally bind haem via only one thioether bridge. A haem lyase gene was found to be specifically required for the maturation of MccA in W. succinogenes. Equivalent haem lyase-encoding genes belonging to either the bacterial cytochrome c biogenesis system I or II are present in the vicinity of every known mccA gene suggesting a dedicated cytochrome c maturation pathway. The results necessitate reconsideration of computer-based prediction of putative haem c-binding motifs in bacterial proteomes
From M-ary Query to Bit Query: a new strategy for efficient large-scale RFID identification
The tag collision avoidance has been viewed as one of the most important research problems in RFID communications and bit tracking technology has been widely embedded in query tree (QT) based algorithms to tackle such challenge. Existing solutions show further opportunity to greatly improve the reading performance because collision queries and empty queries are not fully explored. In this paper, a bit query (BQ) strategy based Mary query tree protocol (BQMT) is presented, which can not only eliminate idle queries but also separate collided tags into many small subsets and make full use of the collided bits. To further optimize the reading performance, a modified dual prefixes matching (MDPM) mechanism is presented to allow multiple tags to respond in the same slot and thus significantly reduce the number of queries. Theoretical analysis and simulations are supplemented to validate the effectiveness of the proposed BQMT and MDPM, which outperform the existing QT-based algorithms. Also, the BQMT and MDPM can be combined to BQMDPM to improve the reading performance in system efficiency, total identification time, communication complexity and average energy cost
Observations of Hunter-Schreger Bands
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66575/2/10.1177_00220345560350052201.pd
Evolution of circular, non-equatorial orbits of Kerr black holes due to gravitational-wave emission: II. Inspiral trajectories and gravitational waveforms
The inspiral of a ``small'' () compact body into a
``large'' () black hole is a key source of
gravitational radiation for the space-based gravitational-wave observatory
LISA. The waves from such inspirals will probe the extreme strong-field nature
of the Kerr metric. In this paper, I investigate the properties of a restricted
family of such inspirals (the inspiral of circular, inclined orbits) with an
eye toward understanding observable properties of the gravitational waves that
they generate. Using results previously presented to calculate the effects of
radiation reaction, I assemble the inspiral trajectories (assuming that
radiation reacts adiabatically, so that over short timescales the trajectory is
approximately geodesic) and calculate the wave generated as the compact body
spirals in. I do this analysis for several black hole spins, sampling a range
that should be indicative of what spins we will encounter in nature. The spin
has a very strong impact on the waveform. In particular, when the hole rotates
very rapidly, tidal coupling between the inspiraling body and the event horizon
has a very strong influence on the inspiral time scale, which in turn has a big
impact on the gravitational wave phasing. The gravitational waves themselves
are very usefully described as ``multi-voice chirps'': the wave is a sum of
``voices'', each corresponding to a different harmonic of the fundamental
orbital frequencies. Each voice has a rather simple phase evolution. Searching
for extreme mass ratio inspirals voice-by-voice may be more effective than
searching for the summed waveform all at once.Comment: 15 pages, 11 figures, accepted for publication in PRD. This version
incorporates referee's comments, and is much less verbos
The dangers of resource myopia in work and organisational psychology: a plea for broadening and integration broadening
In this essay the limitations of the traditional quantitative approach in work and organisational psychology are put forward. It is argued that an extension of the methods, a broadening of the type of problems to be addressed, and a stronger integration with associated disciplines as well as with the application and implementation of the research findings are needed to ensure the usefulness and application of future W&O psychology. It is not suggested that micro-level problems should not be investigated, but it is postulated that W&O psychology should not be deprived of the opportunity to tackle other, and often more relevant, meso-and macro-level issues because we lack appropriate tools for attacking them
Relativistic Calculation of the Meson Spectrum: a Fully Covariant Treatment Versus Standard Treatments
A large number of treatments of the meson spectrum have been tried that
consider mesons as quark - anti quark bound states. Recently, we used
relativistic quantum "constraint" mechanics to introduce a fully covariant
treatment defined by two coupled Dirac equations. For field-theoretic
interactions, this procedure functions as a "quantum mechanical transform of
Bethe-Salpeter equation". Here, we test its spectral fits against those
provided by an assortment of models: Wisconsin model, Iowa State model,
Brayshaw model, and the popular semi-relativistic treatment of Godfrey and
Isgur. We find that the fit provided by the two-body Dirac model for the entire
meson spectrum competes with the best fits to partial spectra provided by the
others and does so with the smallest number of interaction functions without
additional cutoff parameters necessary to make other approaches numerically
tractable. We discuss the distinguishing features of our model that may account
for the relative overall success of its fits. Note especially that in our
approach for QCD, the resulting pion mass and associated Goldstone behavior
depend sensitively on the preservation of relativistic couplings that are
crucial for its success when solved nonperturbatively for the analogous
two-body bound-states of QED.Comment: 75 pages, 6 figures, revised content
Multihospital outbreak of Clostridium difficile ribotype 027 infection: Epidemiology and analysis of control measures
Scale-free static and dynamical correlations in melts of monodisperse and Flory-distributed homopolymers: A review of recent bond-fluctuation model studies
It has been assumed until very recently that all long-range correlations are
screened in three-dimensional melts of linear homopolymers on distances beyond
the correlation length characterizing the decay of the density
fluctuations. Summarizing simulation results obtained by means of a variant of
the bond-fluctuation model with finite monomer excluded volume interactions and
topology violating local and global Monte Carlo moves, we show that due to an
interplay of the chain connectivity and the incompressibility constraint, both
static and dynamical correlations arise on distances . These
correlations are scale-free and, surprisingly, do not depend explicitly on the
compressibility of the solution. Both monodisperse and (essentially)
Flory-distributed equilibrium polymers are considered.Comment: 60 pages, 49 figure
Distribution and fluctuations of backswimmers (Notonectidae) in a tropical shallow lake and predation on microcrustaceans
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