Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation

BACKGROUND: Ischemia within the optic nerve head (ONH) may contribute to retinal ganglion cell (RGC) loss in primary open angle glaucoma (POAG). Ischemia has been reported to increase neurotrophin and high affinity Trk receptor expression by CNS neurons and glial cells. We have previously demonstrated neurotrophin and Trk expression within the lamina cribrosa (LC) region of the ONH. To determine if ischemia alters neurotrophin and Trk protein expression in cells from the human LC, cultured LC cells and ONH astrocytes were exposed to 48 hours of oxygen-glucose deprivation (OGD). Also cells were exposed to 48 hours of OGD followed by 24 hours of recovery in normal growth conditions. Cell number, neurotrophin and Trk receptor protein expression, neurotrophin secretion, and Trk receptor activation were examined. RESULTS: Cell number was estimated using an assay for cell metabolism following 24, 48 and 72 hours of OGD. A statistically significant decrease in LC and ONH astrocyte cell number did not occur until 72 hours of OGD, therefore cellular protein and conditioned media were collected at 48 hours OGD. Protein expression of NGF, BDNF and NT-3 by LC cells and ONH astrocytes increased following OGD, as did NGF secretion. Recovery from OGD increased BDNF protein expression in LC cells. In ONH astrocytes, recovery from OGD increased NGF protein expression, and decreased BDNF secretion. Trk A expression and activation in LC cells was increased following OGD while expression and activation of all other Trk receptors was decreased. A similar increase in Trk A expression and activation was observed in ONH astrocytes following recovery from OGD. CONCLUSIONS: In vitro conditions that mimic ischemia increase the expression and secretion of neurotrophins by cells from the ONH. Increased Trk A expression and activation in LC cells following OGD and in ONH astrocytes following recovery from OGD suggest autocrine/paracrine neurotrophin signaling could be a response to ONH ischemia in POAG. Also, the increase in NGF, BDNF and NT-3 protein expression and NGF secretion following OGD also suggest LC cells and ONH astrocytes may be a paracrine source of neurotrophins for RGCs

Matrix models as solvable glass models

We present a family of solvable models of interacting particles in high dimensionalities without quenched disorder. We show that the models have a glassy regime with aging effects. The interaction is controlled by a parameter $p$. For $p=2$ we obtain matrix models and for $p>2$ `tensor' models. We concentrate on the cases $p=2$ which we study analytically and numerically.Comment: 10 pages + 2 figures, Univ.Roma I, 1038/94, ROM2F/94/2

On the relation between effective supersymmetric actions in different dimensions

We make two remarks: (i) Renormalization of the effective charge in a 4--dimensional (supersymmetric) gauge theory is determined by the same graphs and is rigidly connected to the renormalization of the metric on the moduli space of the classical vacua of the corresponding reduced quantum mechanical system. Supersymmetry provides constraints for possible modifications of the metric, and this gives us a simple proof of nonrenormalization theorems for the original 4-dimensional theory. (ii) We establish a nontrivial relationship between the effective (0+1)-dimensional and (1+1)-dimensional Lagrangia (the latter represent conventional Kahlerian sigma models).Comment: 15 pages, 2 figure

Gravitational Violation of R Parity and its Cosmological Signatures

The discrete R-parity ($R_P$) usually imposed on the Supersymmetric (SUSY) models is expected to be broken at least gravitationally. If the neutralino is a dark matter particle its decay channels into positrons, antiprotons and neutrinos are severely constrained from astrophysical observations. These constraints are shown to be violated even for Planck-mass-suppressed dimension-five interactions arising from gravitational effects. We perform a general analysis of gravitationally induced $R_P$ violation and identify two plausible and astrophysically consistent scenarios for achieving the required suppression.Comment: 10 pages, no figure

Solutions to the cosmological constant problems

We critically review several recent approaches to solving the two cosmological constant problems. The "old" problem is the discrepancy between the observed value of $\Lambda$ and the large values suggested by particle physics models. The second problem is the "time coincidence" between the epoch of galaxy formation $t_G$ and the epoch of $\Lambda$-domination t_\L. It is conceivable that the "old" problem can be resolved by fundamental physics alone, but we argue that in order to explain the "time coincidence" we must account for anthropic selection effects. Our main focus here is on the discrete-$\Lambda$ models in which $\Lambda$ can change through nucleation of branes. We consider the cosmology of this type of models in the context of inflation and discuss the observational constraints on the model parameters. The issue of multiple brane nucleation raised by Feng {\it et. al.} is discussed in some detail. We also review continuous-\L models in which the role of the cosmological constant is played by a slowly varying potential of a scalar field. We find that both continuous and discrete models can in principle solve both cosmological constant problems, although the required values of the parameters do not appear very natural. M-theory-motivated brane models, in which the brane tension is determined by the brane coupling to the four-form field, do not seem to be viable, except perhaps in a very tight corner of the parameter space. Finally, we point out that the time coincidence can also be explained in models where $\Lambda$ is fixed, but the primordial density contrast $Q=\delta\rho/\rho$ is treated as a random variable.Comment: 30 pages, 3 figures, two notes adde

Bounds on charged higgs boson in the 2HDM type III from Tevatron

We consider the Two Higgs Doublet Model (2HDM) of type III which leads to Flavour Changing Neutral Currents (FCNC) at tree level. In the framework of this model we can use an appropriate form of the Yukawa Lagrangian that makes the type II model limit of the general type III couplings apparent. This way is useful in order to compare with the experimental data which is model dependent. The analytical expressions of the partial width $\Gamma (t \to H^+ b)$ are derived and we compare with the data available at this energy range. We examine the limits on the new parameters $\lambda_{ij}$ from the validness of perturbation theory.Comment: 14 pages, 4 figures, Universidad Nacional de Colombia. typos correcte