Epidemiology, prevention and control of oral diseases in pediatric subjects

Caries and periodontal diseases are a neglected epidemic by millions of Italians who suffer unnecessarily from them. Both diseases are preventable and the combination of high prevalence, high morbidity and relative inattention from the National Health Service (NHS), makes caries and periodontal disease a significant public health problem. In this perspective, the World Health Organization and the University of Milan have promoted a national epidemiological study aimed at a sample of 4, 6 and 12-years-old students from schools in the North, Central and South of Italy. The study provides for the administration of a questionnaire to parents and a dental examination carried out at school by dentists trained by the WHO. In our town the epidemiological study planning and the survey were carried out by professors of the School of Specialization in "Pediatric Dentistry" (Prof. G. Giuliana and Prof. G. Pizzo) supported by specialist dentists from the same School, calibrated according to the WHO methodology. The survey was carried out between 2017 and 2019 involving 2701 children attending schools in the 8 districts of the town. The parents were sent a letter which explained the nature of the survey and contained a pre-printed form for the issue of informed consent to participate in the survey with a questionnaire regarding the family nuclear, the oral hygiene and eating habits of the minor and the child's previous dental experiences. The ICDAS (International Caries Detection and Assessment System) was used to detect the prevalence of carious lesions. The dentist used for each child visited a dental mirror and the CPI (Community Periodontal Index) index for periodontal assessment. Preliminary results showed that 25.3% of 12-years-old students had gingival bleeding and 41.1% had tartar. The caries index (ICDAS) shows us that as early as 4 years, 20% of children have initial carious lesions, 37% have dentine caries and that 34.38% have highly destructive caries. At 12 years, 34.95% had initial carious lesions, 46% had cavities that already affected dentin and that 42.44% had highly destructive cavities. The exigence to implement a program of prevention / early treatment of caries/gingivitis in pediatric subjects living in Palermo, since the first months of child's life, was born from the data analysis. The project "Un sorriso per 54 tutti i bambini", carried out at the U.O. of "Pediatric Dentistry" of Policlinico Paolo Giaccone in Palermo, was therefore finalised in order to 1) improve efficiency and effectiveness in the promotion of the oral health of minors, through the creation of a preventive / assistance route of significant social health impact; 2) assess the effectiveness of the preventive methodologies adopte

New radio halos and relics in clusters of galaxies

We present here new images of relics and halo sources in rich cluster of galaxies and the correlation between the halo radio surface brightness versus the cluster bolometric X-ray luminosity.Comment: 2 pages, 4 figures. To appear in the Proceedings of the International Conference: "The Origin and Evolution of Cosmic Magnetism"; Bologna 29 August - 2 September 2005; eds R. Beck, G. Brunetti, L. Feretti, and B. Gaensler (Astronomische Nachrichten, 2006

Stream dynamics between 1 AU and 2 AU: A detailed comparison of observations and theory

A radial alignment of three solar wind stream structures observed by IMP-7 and -8 (at 1.0 AU) and Voyager 1 and 2 (in the range 1.4 to 1.8 AU) in late 1977 is presented. It is demonstrated that several important aspects of the observed dynamical evolution can be both qualitatively and quantitatively described with a single-fluid 2-D MHD numerical model of quasi-steady corotating flow, including accurate prediction of: (1) the formation of a corotating shock pair at 1.75 AU in the case of a simple, quasi-steady stream; (2) the coalescence of the thermodynamic and magnetic structures associated with the compression regions of two neighboring, interacting, corotating streams; and (3) the dynamical destruction of a small (i.e., low velocity-amplitude, short spatial-scale) stream by its overtaking of a slower moving, high-density region associated with a preceding transient flow. The evolution of these flow systems is discussed in terms of the concepts of filtering and entrainment

Invariant NKT cells and rheumatic disease: Focus on primary sjogren syndrome

Primary Sjogren syndrome (pSS) is a complex autoimmune disease mainly affecting salivary and lacrimal glands. Several factors contribute to pSS pathogenesis; in particular, innate immunity seems to play a key role in disease etiology. Invariant natural killer (NK) T cells (iNKT) are a T-cell subset able to recognize glycolipid antigens. Their function remains unclear, but studies have pointed out their ability to modulate the immune system through the promotion of specific cytokine milieu. In this review, we discussed the possible role of iNKT in pSS development, as well as their implications as future markers of disease activity

Diffuse radio emission in the complex merging galaxy cluster Abell 2069

Galaxy clusters with signs for a recent merger show in many cases extended diffuse radio features. This emission originates from relativistic electrons which suffer synchrotron losses due to the intra-cluster magnetic field. The mechanisms of the particle acceleration and the properties of the magnetic field are still poorly understood. We search for diffuse radio emission in galaxy clusters. Here, we study the complex galaxy cluster Abell 2069, for which X-ray observations indicate a recent merger. We investigate the cluster's radio continuum emission by deep Westerbork Synthesis Radio Telescope (WSRT) observations at 346 MHz and a Giant Metrewave Radio Telescope (GMRT) observation at 322 MHz. We find an extended diffuse radio feature roughly coinciding with the main component of the cluster. We classify this emission as a radio halo and estimate its lower limit flux density to 25 +/- 9 mJy. Moreover, we find a second extended diffuse source located at the cluster's companion and estimate its flux density to 15 +/- 2 mJy. We speculate that this is a small halo or a mini-halo. If true, this cluster is the first example of a double-halo in a single galaxy cluster.Comment: 6 pages, 3 figures, accepted for publication in A&

Chelation of cytoplasmic Ca2+ increases plasma membrane permeability in murine macrophages.

Cytoplasmic free Ca2+ (Ca2+i) was chelated to 10-20 nM in the macrophage cell line J774 either by incubation with quin2 acetoxymethyl ester in the absence of external Ca2+ (Di Virgilio, F., Lew, P.D., and Pozzan, T. (1984) Nature 310, 691-693) or by loading [ethyl-enebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) into the cytoplasm via reversible permeabilization of the plasma membrane with extracellular ATP (Steinberg, T.H., Newman, A.S., Swanson, J.A., and Silverstein, SS.C. (1987) J. Biol. Chem. 262, 8884-8888; Di Virgilio, F., Meyer, B.C., Greenberg, S., and Silverstein, S.C. (1988) J. Cell Biol. 106, 657-666). After removal of ATP from the incubation medium, ATP-permeabilized Ca2+i-depleted macrophages recovered a near-normal plasma membrane potential which slowly depolarized over a 2-4 h incubation at low [Ca2+]i. In both ATP-treated and quin2-loaded cells, depolarization of plasma membrane potential was paralleled by an increase in plasma membrane permeability to low molecular weight aqueous solutes such as eosin yellowish (Mr 692), ethidium bromide (Mr 394), and lucifer yellow (Mr 463). This increased plasma membrane permeability was not accompanied by release of the cytoplasmic marker lactic dehydrogenase for incubations up to 4 h and was likely a specific effect of Ca2+i depletion since it was not caused by: (i) the mere incubation of macrophages with extracellular EGTA, i.e. at near-normal [Ca2+]i; and (ii) loading into the cytoplasm of diethylenetriaminepentaacetic acid, a specific chelator of heavy metals with low affinity for Ca2+. Treatment of Ca2+i-depleted cells with direct (phorbol 12-myristate 13-acetate) or indirect (platelet-activating factor) activators of protein kinase C prevented the increase in plasma membrane permeability. Down-regulation of protein kinase C rendered Ca2+i-depleted macrophages refractory to the protective effect of phorbol 12-myristate 13-acetate. This report suggests a role for Ca2+i and possibly protein kinase C in the regulation of plasma membrane permeability to low molecular weight aqueous solutes

IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS

Information on the potential effects of fusariotoxins on reproductive system in ruminants are few. Therefore, the purpose of this study is to evaluate if DON and ZEA major hydroxylated metabolites, \u3b1-zearalenol (\u3b1-Zol) and \u3b2-zearalenone (\u3b2-Zol), alone or in combination, can impair bovine reproductive activity via affecting cell proliferation, estradiol (E2) and progesterone (P4) production and gene expression (CYP11A1 and CYP19A1 mRNA abundance) of primary granulosa cells (GC). GC were obtained aspirating aseptically small (1 to 5 mm) (SMGC) and large (8-22 mm) (LGGC) ovarian follicles. GC were cultured for 2 days in medium containing 10% fetal bovine serum followed by 1 or 2 days in serum free medium without (control) or with added treatments. At the end of the experiments, numbers of GC were determined using a Coulter counter, the concentration of steroid hormones was evaluated via radioimmunoassay and RNA analysis was performed. In SMGC cell proliferation was negatively affected after exposure to \u3b2-Zol at 31 \ub5M and after exposure to \u3b1-Zol (3.1 \ub5M) alone and combined with DON (3.3 \ub5M). Steroidogenesis was differently affected by tested mycotoxins: DON and \u3b1-Zol had inhibitory effects while \u3b2-Zol at high concentration (31 \ub5M) stimulated steroid production. DON (3.3 \ub5M) and \u3b2-Zol (31 \ub5M) increased GC CYP19A1 mRNA abundance. CYP11A1 mRNA abundance was stimulated by DON, alone and combined with \u3b1-Zol and \u3b2-Zol, whereas was inhibited by \u3b2-Zol alone. In LGGC, \u3b1-Zol alone and in combination with DON, increased cell proliferation whereas DON strongly inhibited P4 and E2 production mainly at high doses (3.3 \ub5M). In conclusion these results demonstrated that DON and ZEA metabolites may affect in vitro bovine GC function and also provides information on the interaction between DON and ZEA metabolites

Contributions to the Fourth Solar Wind Conference

Recent results in interplanetary physics are examined. These include observations of shock waves and post-shock magnetic fields made by Voyager 1, 2; observations of the electron temperature as a function of distance between 1.36 AU and 2.25 AU; and observations of the structure of sector boundaries observed by Helios 1. A theory of electron energy transport in the collisionless solar wind is presented, and compared with observations. Alfven waves and Alvenic fluctuations in the solar wind are also discussed