Testing for hybridisation of the Critically Endangered Iguana delicatissima on Anguilla to inform conservation efforts

The Caribbean Island of Anguilla in the north-eastern Lesser Antilles is home to one of the last populations of the Critically Endangered Lesser Antillean iguana Iguana delicatissima. This population is highly threatened primarily because of hybridisation with non-native Iguana iguana. This study assesses the degree of hybridisation between Anguilla’s Iguana species firstly using morphological characteristics and then genetic analysis to validate the genetic integrity of morphologically identified I. delicatissima. We also examined the genetic diversity of Anguilla’s I. delicatissima population, and that of a population on the nearby island of Îlet Fourchue, St Barthélemy. Forty-five iguanas were captured in Anguilla and 10 in St Barthélemy, and sequences from 3 nuclear and 1 mtDNA genes were obtained for each. Of the 45 iguanas captured in Anguilla, 22 were morphologically identified as I. delicatissima, 12 as I. iguana and the remainder were identified as hybrids. Morphological assignments were all confirmed by genetic analyses except for one I. iguana and one hybrid individual. These two individuals appeared likely to have originated following ancestral hybridisation events several generations ago. A significant paucity of genetic diversity was found within Anguillan and St Barthélemy I. delicatissima populations, with a single haplotype being identified for each of the three nuclear genes and the mtDNA sequence. This study highlights the urgency for immediate action to conserve Anguilla’s remnant I. delicatissima population. Protection from hybridisation will require translocation to I. iguana-free offshore cays, with supplementary individuals being sourced from neighbouring islands to enhance the genetic diversity of the population

Opportunity mapping for nature-based solutions: Mitigating storm surge and land erosion in the Caribbean

The islands of the Caribbean are particularly susceptible to the effects of climate change due to their low-lying coastal areas and location within the Atlantic basin's hurricane belt. The UK Overseas Territory of Anguilla is one such island. The predicted increase in the severity of hurricanes and sea-level rise is highly likely to increase the flood risk of already vulnerable island communities. In this study, flood risk and erosion models are used to prioritise opportunity areas for nature-based restoration and to identify those that would have the greatest impact on coastal and in-land flood risk reduction. Two study sites in Anguilla were selected to highlight this ecologically-based modelling approach; Cove Bay and Pond, a degraded sand dune system and brackish pond, and the East End Pond, an Important Bird and Biodiversity Area that floods following heavy rainfall events. At the coastal site, the restoration of mangroves, sand dunes and coral reefs have the potential to provide flood risk reduction up to 500 m inland and protect homes, infrastructure and tourism developments. For the in-land East End Pond, areas of high erosion risk were predominately identified as bare or disturbed land within 1 km of the pond's basin. Habitat restoration of these areas was identified as having the greatest impact on reducing flood risk. The creation of flood risk, opportunity and impact models are invaluable tools that can be used to inform, advocate and justify the implementation of nature-based solutions to a range of stakeholders from policy-makers to local communties

Saving the sea turtles of Anguilla: Combining scientific data with community perspectives to inform policy decisions

Historic over-exploitation and the more recent threats caused by fisheries by-catch, disease and climate change have left sea turtle populations in the Wider Caribbean at risk of extinction. In 1995, following regional declines in nesting and foraging populations, the island of Anguilla implemented a moratorium on the hunting of turtles. At the request of the Government of Anguilla for scientific data to either support or remove the moratorium, comprehensive population estimates were obtained, and foraging, nesting and migratory movements were examined. In addition, community perspectives on turtles and their protection were assessed. Between 2015 and 18 surveys of 30 nesting beaches estimated low nesting activity with a maximum of 41 hawksbill, 15 green, and 1–2 leatherback turtles nesting in Anguilla annually. The inter-nesting range of hawksbills exhibited high levels of geographic overlap and occurred within 1.5 km of nesting beaches. Migratory tracks of hawksbill turtles traversed through seven exclusive economic zones, two of which allow a legal turtle fishery. Site fidelity was observed in foraging areas of green turtles and genetic analysis revealed population differentiation between green turtle foraging sites in Anguilla and between hawksbill rookeries in Anguilla compared to other Leeward Islands, indicating the individual importance of each foraging and nesting site. The Anguillan public (n = 302) overwhelmingly agreed with the current ban on harvesting sea turtles and considered turtles important for ecotourism. Our work provides a case-study, that can be applied globally, of how scientific research combined with community perspectives can effectively inform policy and ultimately protect endangered species, and highlights that local Governments provided with high quality data in a timely fashion for their policy making timetable are more likely to integrate findings into their decision-making process

Orthotopic transplantation of immortalized mesencephalic progenitors (CSM14.1 cells) into the substantia nigra of hemiparkinsonian rats induces neuronal differentiation and motoric improvement

Neural progenitor cell grafting is a promising therapeutic option in the treatment of Parkinson's disease. In previous experiments we grafted temperature-sensitive immortalized CSM14.1 cells, derived from the ventral mesencephalon of E14-rats, bilaterally in the caudate putamen of adult hemiparkinsonian rats. In these studies we were not able to demonstrate either a therapeutic improvement or neuronal differentiation of transplanted cells. Here we examined whether CSM14.1 cells grafted bilaterally orthotopically in the substantia nigra of hemiparkinsonian rats have the potential to differentiate into dopaminergic neurons. Adult male rats received 6-hydroxydopamine into the right medial forebrain bundle, and successful lesions were evaluated with apomorphine-induced rotations 12 days after surgery. Two weeks after a successful lesion the animals received bilateral intranigral grafts consisting of either about 50 000 PKH26-labelled undifferentiated CSM14.1 cells (n = 16) or a sham-graft (n = 9). Rotations were evaluated 3, 6, 9 and 12 weeks post-grafting. Animals were finally perfused with 4% paraformaldehyde. Cryoprotected brain slices were prepared for immunohistochemistry using the freeze-thaw technique to preserve PKH26-labelling. Slices were immunostained against neuronal epitopes (NeuN, tyrosine hydroxylase) or glial fibrillary acidic protein. The CSM14.1-cell grafts significantly reduced the apomorphine-induced rotations 12 weeks post-grafting compared to the sham-grafts (P < 0.05). There was an extensive mediolateral migration (400–700 µm) of the PKH26-labelled cells within the host substantia nigra. Colocalization with NeuN or glial fibrillary acidic protein in transplanted cells was confirmed with confocal microscopy. No tyrosine hydroxylase-immunoreactive grafted cells were detectable. The therapeutic effect of the CSM14.1 cells could be explained either by their glial cell-derived neurotrophic factor-expression or their neural differentiation with positive effects on the basal ganglia neuronal networks