Mol. Cell. Proteomics

Chemical cross-linking in combination with mass spectrometric analysis offers the potential to obtain low-resolution structural information from proteins and protein complexes. Identification of peptides connected by a cross-link provides direct evidence for the physical interaction of amino acid side chains, information that can be used for computational modeling purposes. Despite impressive advances that were made in recent years, the number of experimentally observed cross-links still falls below the number of possible contacts of cross-linkable side chains within the span of the cross-linker. Here, we propose two complementary experimental strategies to expand cross-linking data sets. First, enrichment of cross-linked peptides by size exclusion chromatography selects cross-linked peptides based on their higher molecular mass, thereby depleting the majority of unmodified peptides present in proteolytic digests of cross-linked samples. Second, we demonstrate that the use of proteases in addition to trypsin, such as Asp-N, can additionally boost the number of observable cross-linking sites. The benefits of both SEC enrichment and multiprotease digests are demonstrated on a set of model proteins and the improved workflow is applied to the characterization of the 20S proteasome from rabbit and Schizosaccharomyces pombe

Central Acceptance Testing for Camera Technologies for CTA

The Cherenkov Telescope Array (CTA) is an international initiative to build the next generation ground based very-high energy gamma-ray observatory. It will consist of telescopes of three different sizes, employing several different technologies for the cameras that detect the Cherenkov light from the observed air showers. In order to ensure the compliance of each camera technology with CTA requirements, CTA will perform central acceptance testing of each camera technology. To assist with this, the Camera Test Facilities (CTF) work package is developing a detailed test program covering the most important performance, stability, and durability requirements, including setting up the necessary equipment. Performance testing will include a wide range of tests like signal amplitude, time resolution, dead-time determination, trigger efficiency, performance testing under temperature and humidity variations and several others. These tests can be performed on fully-integrated cameras using a portable setup at the camera construction sites. In addition, two different setups for performance tests on camera sub-units are being built, which can provide early feedback for camera development. Stability and durability tests will include the long-term functionality of movable parts, water tightness of the camera housing, temperature and humidity cycling, resistance to vibrations during transport or due to possible earthquakes, UV-resistance of materials and several others. Some durability tests will need to be contracted out because they will need dedicated equipment not currently available within CTA. The planned test procedures and the current status of the test facilities will be presented.Comment: 8 pages, 3 figures. In Proceedings of the 34th International Cosmic Ray Conference (ICRC2015), The Hague, The Netherlands. All CTA contributions at arXiv:1508.0589

Foetal and Maternal Magnesium Metabolism: Effect of Magnesium Deficiency and Isoproterenol

Peer Reviewe

Quantum molecular dynamics simulations for the nonmetal-to-metal transition in fluid helium

We have performed quantum molecular dynamics simulations for dense helium to study the nonmetal-to-metal transition at high pressures. We present new results for the equation of state and the Hugoniot curve in the warm dense matter region. The optical conductivity is calculated via the Kubo-Greenwood formula from which the dc conductivity is derived. The nonmetal-to-metal transition is identified at about 1 g/ccm. We compare with experimental results as well as with other theoretical approaches, especially with predictions of chemical models.Comment: 4 pages, 5 figure

Analysis of the positive ionospheric response to a moderate geomagnetic storm using a global numerical model

International audienceCurrent theories of F-layer storms are discussed using numerical simulations with the Upper Atmosphere Model, a global self-consistent, time dependent numerical model of the thermosphere-ionosphere-plasmasphere-magnetosphere system including electrodynamical coupling effects. A case study of a moderate geomagnetic storm at low solar activity during the northern winter solstice exemplifies the complex storm phenomena. The study focuses on positive ionospheric storm effects in relation to thermospheric disturbances in general and thermospheric composition changes in particular. It investigates the dynamical effects of both neutral meridional winds and electric fields caused by the disturbance dynamo effect. The penetration of short-time electric fields of magnetospheric origin during storm intensification phases is shown for the first time in this model study. Comparisons of the calculated thermospheric composition changes with satellite observations of AE-C and ESRO-4 during storm time show a good agreement. The empirical MSISE90 model, however, is less consistent with the simulations. It does not show the equatorward propagation of the disturbances and predicts that they have a gentler latitudinal gradient. Both theoretical and experimental data reveal that although the ratio of [O]/[N2] at high latitudes decreases significantly during the magnetic storm compared with the quiet time level, at mid to low latitudes it does not increase (at fixed altitudes) above the quiet reference level. Meanwhile, the ionospheric storm is positive there. We conclude that the positive phase of the ionospheric storm is mainly due to uplifting of ionospheric F2-region plasma at mid latitudes and its equatorward movement at low latitudes along geomagnetic field lines caused by large-scale neutral wind circulation and the passage of travelling atmospheric disturbances (TADs). The calculated zonal electric field disturbances also help to create the positive ionospheric disturbances both at middle and low latitudes. Minor contributions arise from the general density enhancement of all constituents during geomagnetic storms, which favours ion production processes above ion losses at fixed height under day-light conditions

Review of SIS Experimental Results on Strangeness

>A review of meson emission in heavy ion collisions at incident energies around 1 -- 2 $A\cdot$GeV is presented. It is shown how the shape of the spectra and the various particle yields vary with system size, with centrality and with incident energy. A statistical model assuming thermal and chemical equilibrium and exact strangeness conservation (i.e. strangeness conservation per collision) explains most of the observed features. Emphasis is put onto the study of $K^+$ and $K^-$ emission. In the framework of this statistical model it is shown that the experimentally observed equality of $K^+$ and $K^-$ rates at threshold corrected energies $\sqrt{s} - \sqrt{s_{th}}$ is due to a crossing of two excitation functions. Furthermore, the independence of the $K^+$ to $K^-$ ratio on the number of participating nucleons observed between 1 and 10 $A\cdot$GeV is consistent with this model. The observed flow effects are beyond the scope of this model.Comment: 10 pages, 9 figures, Strangeness 2000, V International Conference on Strangeness in Quark Matter, July, 2000, Berkeley, Californi

Precision preparation of strings of trapped neutral atoms

We have recently demonstrated the creation of regular strings of neutral caesium atoms in a standing wave optical dipole trap using optical tweezers [Y. Miroshnychenko et al., Nature, in press (2006)]. The rearrangement is realized atom-by-atom, extracting an atom and re-inserting it at the desired position with sub-micrometer resolution. We describe our experimental setup and present detailed measurements as well as simple analytical models for the resolution of the extraction process, for the precision of the insertion, and for heating processes. We compare two different methods of insertion, one of which permits the placement of two atoms into one optical micropotential. The theoretical models largely explain our experimental results and allow us to identify the main limiting factors for the precision and efficiency of the manipulations. Strategies for future improvements are discussed.Comment: 25 pages, 18 figure