Overexpression of water-responsive genes promoted by elevated CO2 reduces ROS and enhances drought tolerance in Coffea species

Drought is a major constraint to plant growth and productivity worldwide and will aggravate as water availability becomes scarcer. Although elevated air [CO2] might mitigate some of these effects in plants, the mechanisms underlying the involved responses are poorly understood in woody economically important crops such as Coffea. This study analyzed transcriptome changes in Coffea canephora cv. CL153 and C. arabica cv. Icatu exposed to moderate (MWD) or severe water deficits (SWD) and grown under ambient (aCO2) or elevated (eCO2) air [CO2]. We found that changes in expression levels and regulatory pathways were barely affected by MWD, while the SWD condition led to a down-regulation of most differentially expressed genes (DEGs). eCO2 attenuated the impacts of drought in the transcripts of both genotypes but mostly in Icatu, in agreement with physiological and metabolic studies. A predominance of protective and reactive oxygen species (ROS)-scavenging-related genes, directly or indirectly associated with ABA signaling pathways, was found in Coffea responses, including genes involved in water deprivation and desiccation, such as protein phosphatases in Icatu, and aspartic proteases and dehydrins in CL153, whose expression was validated by qRT-PCR. The existence of a complex post-transcriptional regulatory mechanism appears to occur in Coffea explaining some apparent discrepancies between transcriptomic, proteomic, and physiological data in these genotypes.info:eu-repo/semantics/publishedVersio

Agronomic performance and genetic divergence between genotypes of Manihot esculenta

The morphoagronomic characterization of 12 genotypes of M. esculenta was performed during the 2013/2014 and 2014/2015 crop years. The 12 genotypes were planted in a randomized block design, with four replicates per genotype. Number of tuberous roots per plant, weight of tuberous roots, root yield, total plant weight, harvest index, plant height, height of first branch, number of shoots, stem diameter, number of buds, leaf dry weight and petiole length were evaluated. Genotypes “Camuquem” and “Goiás” were the most productive, and “Amarela” and “Gema de Ovo” were the most divergent. Seventy percent of genetic diversity was due to petiole length (22.86%), root yield (19.20%), weight of tuberous roots (14.89%) and number of buds (13.72%). Overall, the present results indicate a broad genetic basis for the evaluated genotypes, so that such genetic variation benefits the plant breeding for future scenarios Further studies of the evaluated genotypes should be performed under environmental limitations, using biochemical and molecular tools to identify markers for genetic improvementinfo:eu-repo/semantics/publishedVersio

Understanding the Impact of Drought in Coffea Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit

Water scarcity is the most significant factor limiting coffee production, although some cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of two coffee cultivars with contrasting physiological responses, Coffea canephora cv. CL153 and Coffea. arabica cv. Icatu, subjected to moderate (MWD) or severe water deficits (SWD). We found that MWD had a low impact compared with SWD, where 10% of all genes in Icatu and 17% in CL153 reacted to drought, being mainly down-regulated upon stress. Drought triggered a genotype-specific response involving the up-regulation of reticuline oxidase genes in CL153 and heat shock proteins in Icatu. Responsiveness to drought also included desiccation protectant genes, but primarily, aspartic proteases, especially in CL153. A total of 83 Transcription Factors were found engaged in response to drought, mainly up-regulated, especially under SWD. Together with the enrollment of 49 phosphatases and 272 protein kinases, results suggest the involvement of ABA-signaling processes in drought acclimation. The integration of these findings with complementing physiological and biochemical studies reveals that both genotypes are more resilient to moderate drought than previously thought and suggests the existence of post-transcriptional mechanisms modulating the response to drought.info:eu-repo/semantics/publishedVersio

Biometric traits as a tool for the identification and breeding of coffea canephora genotypes

grants n. 84320893 grants n. 420789/2016-2 n. 304687/2017-0 E-26/202.323/2017 UID/04129/2020 UIDP/04035/2020Cross-pollination and gametophytic self-incompatibility reduce the stability of Coffea canephora genotypes. This is an important crop for Brazil, the largest producer of this type of coffee and also a major exporter. The study of biometric characteristics is essential to assist in the selection of promising plant materials. We examined the diversity of morpho-agronomic traits of genotypes of C. canephora cv. Conilon through the evaluation of branch and leaf parameters. Assessments included plagiotropic branch length, number of nodes in plagiotropic branches, distance between nodes in plagiotropic branches, orthotropic branch length, number of nodes in orthotropic branch, distance between nodes in orthotropic branch, plant height, canopy diameter, leaf length, leaf width, and leaf area in two periods. The data from the 43 coffee genotypes were tested by multivariate and cluster analyses. Six groups were formed by the Tocher optimization method, and five groups by the unweighted pair group method with arithmetic mean (UPGMA) hierarchical method, suggesting an important genetic variability among plant materials. Both Tocher optimization and UPGMA hierarchical methods were consistent for clustering the genotypes, ordering them in six and five dissimilar groups, respectively, with genotypes 25 and 37 standing out with the greatest dissimilarity, constituting isolated groups by both methods. Pearson’s correlation ranged from very weak to very strong, positive and negative, among the characteristics, as also shown by principal component analyses. These analyses indicated the morpho-agronomic traits with a greater degree of correlation, assisting in the choice of promising plant materials. The genetic parameters estimates demonstrate genetic variability and thus breeding potential within the Conilon coffee genotypes studied. These results emphasize the usefulness of biometric evaluations as a tool for the identification and breeding of genotypes to compose new Conilon coffee cultivars.publishersversionpublishe

A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora

Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 C) than previously assumed.info:eu-repo/semantics/publishedVersio

Treinamento resistido com oclusão vascular e respostas adaptativas em idosos hipertensos controlados / Resisted training with vascular occlusion and adaptive responses in controlled hypertensive elderly

O envelhecimento está associado ao declínio das capacidades fisiológicas, onde se destaca a importância do Treinamento Resistido - TR, evidenciando seus efeitos positivos em comorbidades comuns como a Hipertensão Arterial Sistêmica. O objetivo do estudo foi avaliar as respostas adaptativas do treinamento resistido com Oclusão Vascular - OV em idosos hipertensos controlados. Foram avaliados 07 indivíduos do sexo feminino, idade média de (64,6 ± 4,37 anos), em uma única sessão de treinamento resistido com oclusão vascular. A pressão utilizada para a oclusão foi de 200mmHg, além da utilização de exercícios para membros inferiores com carga ajustada em 30% de 1RM. Antes durante e após a sessão foram realizadas mensurações dos parâmetros hemodinâmicos, sendo eles: Pressão Arterial Sistólica - PAS, Pressão Arterial Diastólica – PAD e Frequência Cardíaca – FC. Através desta variáveis foram calculadas a Pressão Arterial Média - PAM e Duplo Produto - DP. Assim, conclui-se que o TR se apresentou como opção viável e segura para este grupo, em especial, pelo uso de baixas cargas estimadas para o exercício

Impact of climate changes in the suitable areas for Coffea arabica L. production in Mozambique: Agroforestry as an alternative management system to strengthen crop sustainability

Climate changes (CC) are a main global phenomenon, with a worldwide impact on natural and agricultural ecosystems. The objective of this study was to analyse the potential impact of future CC on the suitability of areas for rainfed coffee growth, both at the Mozambique national scale and in the Gorongosa Mountain, under Agroforestry (AFS) and Full Sun (FS) management systems. The latter study site is part of the Gorongosa National Park (PNG), one of the most biodiverse places and an outstanding case of successful ecosystem restoration, including the rainforest from Gorongosa Mountain. Additionally, coffee cultivation in PNG under AFS is part of a strategy to strengthen the socio-economic sustainability of the local population, and the recovery of biodiversity in a degraded tropical rainforest ecosystem. Future climate assessments were elaborated through bioclimatic and biophysical variables (Elevation), with Coffea arabica L. being modeled under the current conditions and four global climate models (GCMs) using four Shared Socio-economic Pathways (SSPs). Isothermality, annual precipitation, and altitude were the most important variables influencing suitable areas in Mozambique. The analysis revealed that currently suitable areas where C. arabica is grown in Mozambique will be negatively affected under future scenarios (SSP126 to SSP585) in both systems (AFS and FS), although with clear worst impacts for FS. Under AFS, suitable areas will be reduced between about half and two-thirds by 2041–2060, and up to 91% by 2081–2100 (depending on scenarios) at the whole country level. Additionally, in Gorongosa Mountain, almost all scenarios point to a 30% reduction of the suitable area by 2041–2060, reaching 50% by 2081–2100, both in SSP126 and SSP245 scenarios. In sharp contrast, at the whole country level, the FS system is projected to be unsuitable for most of Mozambique, with area losses close to or above two-thirds already in 2021–2040, and greater than 80% by 2061–2080. Under this system, the projections were even more dramatic, pointing to a total absence of adequate areas at Gorongosa Mountain already by 2021–2040. Overall, our study provides clear evidence that the implementation of AFS greatly reduces CC deleterious impacts, being crucial to guarantee the sustainability of the coffee crop in the near future.info:eu-repo/semantics/publishedVersio

Agroforestry as an alternative management system to strengthen crop sustainability

Funding Information: This work was supported by national funds of Camões – Instituto da Cooperação e da Língua (Portugal), Agência Brasileira de Cooperação (Brazil), and Parque Nacional da Gorongosa (Mozambique), through the project GorongosaCafé (TriCafé), as well as through Portuguese national funds from Fundação para a Ciência e a Tecnologia, I.P. (FCT), through the grants SFRH/BD/135357/2017 (C. Cassamo), and the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (I. Marques), and to the research units UIDB/00239/2020 (CEF), UIDB/00329/2020 (CE3C) and UIDP/04035/2020 (GeoBioTec). Funding from CNPq, Brazil (F. Partelli) is also greatly acknowledged. Funding Information: This work was supported by national funds of Camões – Instituto da Cooperação e da Língua (Portugal), Agência Brasileira de Cooperação (Brazil), and Parque Nacional da Gorongosa (Mozambique), through the project GorongosaCafé (TriCafé), as well as through Portuguese national funds from Fundação para a Ciência e a Tecnologia , I.P. (FCT), through the grants SFRH/BD/135357/2017 (C. Cassamo), and the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (I. Marques), and to the research units UIDB/00239/2020 (CEF), UIDB/00329/2020 (CE3C) and UIDP/04035/2020 (GeoBioTec). Funding from CNPq , Brazil (F. Partelli) is also greatly acknowledged. Publisher Copyright: © 2023 The AuthorsClimate changes (CC) are a main global phenomenon, with a worldwide impact on natural and agricultural ecosystems. The objective of this study was to analyse the potential impact of future CC on the suitability of areas for rainfed coffee growth, both at the Mozambique national scale and in the Gorongosa Mountain, under Agroforestry (AFS) and Full Sun (FS) management systems. The latter study site is part of the Gorongosa National Park (PNG), one of the most biodiverse places and an outstanding case of successful ecosystem restoration, including the rainforest from Gorongosa Mountain. Additionally, coffee cultivation in PNG under AFS is part of a strategy to strengthen the socio-economic sustainability of the local population, and the recovery of biodiversity in a degraded tropical rainforest ecosystem. Future climate assessments were elaborated through bioclimatic and biophysical variables (Elevation), with Coffea arabica L. being modeled under the current conditions and four global climate models (GCMs) using four Shared Socio-economic Pathways (SSPs). Isothermality, annual precipitation, and altitude were the most important variables influencing suitable areas in Mozambique. The analysis revealed that currently suitable areas where C. arabica is grown in Mozambique will be negatively affected under future scenarios (SSP126 to SSP585) in both systems (AFS and FS), although with clear worst impacts for FS. Under AFS, suitable areas will be reduced between about half and two-thirds by 2041–2060, and up to 91% by 2081–2100 (depending on scenarios) at the whole country level. Additionally, in Gorongosa Mountain, almost all scenarios point to a 30% reduction of the suitable area by 2041–2060, reaching 50% by 2081–2100, both in SSP126 and SSP245 scenarios. In sharp contrast, at the whole country level, the FS system is projected to be unsuitable for most of Mozambique, with area losses close to or above two-thirds already in 2021–2040, and greater than 80% by 2061–2080. Under this system, the projections were even more dramatic, pointing to a total absence of adequate areas at Gorongosa Mountain already by 2021–2040. Overall, our study provides clear evidence that the implementation of AFS greatly reduces CC deleterious impacts, being crucial to guarantee the sustainability of the coffee crop in the near future.publishersversionpublishe

Measurement of serum lactate values in domestic cats (Felis catus) submitted to physical and chemical restraint

The use of serum lactate has become increasingly common in Veterinary Medicine, since it is an excellent marker of tissue oxygenation, elevated in situations of tissue hypoxia, where anaerobic glycolysis is used as an energy source. Studies point out its use in dogs as a prognostic factor in several affections; however it is not commonly used in domestic felines due to the high stress threshold of the species, which may interfere with their values. The aim of the present study was to evaluate serum lactate values in domestic cats during physical restraint and after chemical restraint using the combination of 8mg/kg ketamine-S, 0.4 mg/kg midazolam, and 2 mg/kg Tramadol. To this end, 20 clinically healthy female cats without defined race, from six months to five years of age, were divided into two groups, GPR (n = 10) represented by felines that had only physical restraint and the GCR (n = 10) by felines that underwent physical restraint (TPR) and after 30 hours by chemical restraint (TCR). It was observed that in both groups (GPR and CPR) during physical restraint presented hyperlactatemia (GPR = 6.23 ± 0.9 mmol/L – GCR – Tpr = 6.66 ± 1.59 mmol/L) and mean values of 4.42 ± 1.28 mmol/L during chemical restraint (GCR – Tcr). It was concluded that physical restraint interferes with serum lactate values, with the reduction of such values with the use of chemical restraint in the feline species.A utilização do lactato sérico é cada vez mais comum na Medicina Veterinária, por ser um excelente marcador de oxigenação tecidual, elevando-se em situações de hipóxia tecidual e realizando a glicólise anaeróbica como fonte de energia. Pesquisas científicas em cães o apontam como fator prognóstico em diversas afecções, sendo pouco utilizado em felinos domésticos devido ao elevado limiar de estresse da espécie, podendo interferir em seus valores. Assim, este trabalho avaliou os valores de lactato sérico em felinos domésticos durante contenção física e após contenção química, utilizando a associação de 8 mg/kg de cetamina-S, 0,4 mg/kg de midazolam e 2 mg/kg de cloridrato de tramadol. Foram estudados 20 felinos, fêmeas, sem raça definida,  de seis meses a cinco anos de idade, clinicamente saudáveis, divididos em dois grupos, com GPR (n = 10) representado por felinos que passaram apenas por contenção física e com GCR por felinos que passaram por contenção física (TPR) e após 30 horas por contenção química (TCR). Foi observado em ambos os grupos (GPR e GCR) e durante a contenção física a hiperlactatemia sérica (GPR = 6.23 ± 0.9 mmol / L – GCR – TPR = 6.66 ± 1.59 mmol / L) com valores médios de 4,42 ± 1,28 mmol/L durante a contenção química (GCR – TCR). Conclui-se que a contenção física interfere nos valores do lactato sérico, com redução dos valores na utilização da contenção química na espécie felina

Primary metabolite profile changes in coffea spp. Promoted by single and combined exposure to drought and elevated co2 concentration

info:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F113475%2F2015/PT PD/00035/2013 CRA-RED-00053-16Climate change scenarios pose major threats to many crops worldwide, including coffee. We explored the primary metabolite responses in two Coffea genotypes, C. canephora cv. Conilon Clone 153 and C. arabica cv. Icatu, grown at normal (aCO2) or elevated (eCO2) CO2 concentrations of 380 or 700 ppm, respectively, under well-watered (WW), moderate (MWD), or severe (SWD) water deficit conditions, in order to assess coffee responses to drought and how eCO2 can influence such responses. Primary metabolites were analyzed with a gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). A total of 48 primary metabolites were identified in both genotypes (23 amino acids and derivatives, 10 organic acids, 11 sugars, and 4 other metabolites), with differences recorded in both genotypes. Increased metabolite levels were observed in CL153 plants under single and combined conditions of aCO2 and drought (MWD and SWD), as opposed to the observed decreased levels under eCO2 in both drought conditions. In contrast, Icatu showed minor differences under MWD, and increased levels (especially amino acids) only under SWD at both CO2 concentration conditions, although with a tendency towards greater increases under eCO2. Altogether, CL153 demonstrated large impact under MWD, and seemed not to benefit from eCO2 in either MWD and SWD, in contrast with Icatu.publishersversionpublishe