Postnatal probiotic supplementation can prevent and optimize treatment of childhood asthma and atopic disorders: a systematic review of randomized controlled trials

Background: Although several randomized controlled trials (RCTs) published over the past 5 years show that prenatal or postnatal probiotics may prevent or optimize the treatment of childhood asthma and atopic disorders, findings from the systematic reviews and meta-analyses of these studies appear inconsistent. More recent RCTs have focused on postnatal probiotics, and linked specific probiotic strains to better disease outcomes. Objective: This systematic review aimed to determine if postnatal probiotics are as effective as prenatal probiotics in preventing or treating childhood asthma and atopic disorders. Methods: We searched the PubMed, Medline, Google Scholar, and EMBASE databases for RCTs published within the past 5 years (from 2017 to 2022). We included only full-text RCTs on human subjects published in or translated into the English language. We retrieved relevant data items with a preconceived data-extraction form and assessed the methodological quality of the selected RCTs using the Cochrane Collaboration's tool for assessing the risk of bias in randomized trials. We qualitatively synthesized the retrieved data to determine any significant differences in study endpoints of the probiotic and placebo groups. Results: A total of 1,320 participants (688 and 632 in the probiotic and placebo groups) from six RCTs were investigated. One RCT showed that early Lactobacillus rhamnosus GG (LGG) led to a reduction in the cumulative incidence rate of asthma. Another study demonstrated that mixed strains of Lactobacillus paracasei and Lactobacillus fermentum could support clinical improvement in children with asthma while one trial reported a significant reduction in the frequency of asthma exacerbations using a mixture of Ligilactobacillus salivarius and Bifidobacterium breve. Three trials showed that a combination of LGG and Bifidobacterium animalis subsp lactis, Lactobacillus rhamnosus alone, and a probiotic mixture of Lactobacillus LOCK strains improved clinical outcomes in children with atopic dermatitis and cow-milk protein allergy. Conclusions: Postnatal strain-specific probiotics (in single or mixed forms) are beneficial in preventing and treating atopic dermatitis and other allergies. Similarly, specific strains are more effective in preventing asthma or improving asthma outcomes. We recommend more interventional studies to establish the most useful probiotic strain in these allergic diseases

Experimental Investigation of the Effect of Sisal Fiber on the Partially Replaced Cement with Groundnut Shell Ash in Concrete

An investigation on the effect of sisal fiber on the partially replaced cement with Groundnut Shell Ash in Concrete was carried out. Sisal fiber of 3.5cm length which forms 1% of the mix by weight with groundnut shell ash as a partial replacement for cement was used in preparing the concrete specimen. Compressive strength test was carried out using 0%, 5%, 10%, 15%, 20%, 25% and 30% of Groundnut shell ash as replacement of cement at different curing ages of 7, 14, 21 and 28 days. It was observed that at 7 and 14days of curing, it is needless introducing the GSA since the maximum compressive strength obtained were at 0% GSA. At 21 and 28days of curing, a considerable increase in compressive strength was observed for 5% and 10% of GSA. However, 5% of GSA can be regarded as the optimum content since it gives the maximum compressive strength value of 30.1N/mm2 at 28days of curing. This is followed by 10% replacement of GSA yielding 28.10N/mm2 and then 0% GSA replacement yielding 25.01N/mm2

High-resolution X-ray Spectra Of The Symbiotic Star SS73 17

SS73 17 was an innocuous Mira-type symbiotic star until Integral and Swift discovered its bright hard X-ray emission, adding it to the small class of "hard X-ray emitting symbiotics." Suzaku observations in 2006 then showed it emits three bright iron lines as well, with little to no emission in the 0.3-2 keV bandpass. We present here followup observations with the Chandra HETG and Suzaku that confirm the earlier detection of strong emission lines of Fe Kalpha fluorescence, Fe XXV and Fe XXVI but also show significantly more soft X-ray emission. The high resolution spectrum also shows emission lines of other highly ionized ions as Si XIV and possibly S XVI. In addition, a reanalysis of the 2006 Suzaku data using the latest calibration shows that the hard (15-50 keV) X-ray emission is brighter than previously thought and remains constant in both the 2006 and 2008 data. The G ratio calculated from the Fe XXV lines shows that these lines are thermal, not photoionized, in origin. With the exception of the hard X-ray emission, the spectra from both epochs can be fit using thermal radiation assuming a differential emission measure based on a cooling flow model combined with a full and partial absorber. We show that acceptable fits can be obtained for all the data in the 1-10 keV band varying only the partial absorber. Based on the temperature and accretion rate, the thermal emission appears to be arising from the boundary layer between the accreting white dwarf and the accretion disk.Comment: 7 pages, 5 figures. Accepted by the Astrophysical Journa

Effect of Total Solids Concentration of Municipal Solid Waste on the Biogas Produced in an Anaerobic Continuous Digester

Municipal Solid Waste (MSW) contains a relatively large amount of organic matter, which decomposes by the actions of microorganisms under anaerobic conditions to produce biogas. The total solids (TS) concentration of the waste influences the pH, temperature and effectiveness of the microorganisms in the decomposition process. This work investigated various concentrations of the TS of MSW in an anaerobic continuously stirred tank reactor (CSTR) and the corresponding amounts of biogas produced, in order to determine conditions for optimum gas production. Five laboratory-scale anaerobic batch digesters of 5 litres volume each were set up for the digestion of 2kg of shredded MSW diluted to a %TS concentration of 26.7%. The results from the batch experimentation were adapted to the design of a CSTR for the digestion of MSW. The CSTR was simulated over a range of %TS concentration of 4-10, at a maximum fractional conversion of 0.8 to cater for system inefficiencies. Mathematical models were developed for the process and solved using the Microsoft Visual Basic version 6.0 Programme. The results show the amount of biogas produced as a power function of the %TS concentration, indicating that as the process continues, a time comes when any marginal increase in the %TS concentration would no longer contribute to the increasing volume of biogas produced. The results further show that, given the limiting upper boundary of the %TS concentration, optimum biogas production in a CSTR digesting MSW cannot be ascertained with the concentration of the %TS alone

Assessment of Selected Physico Chemical Properties of Soil for Site Suitability for Waste Disposal in Abakaliki, Southeast, Nigeria

An assessment of the physicochemical properties of soil for site waste disposal was carried out in Abakaliki urban, southeastern Nigeria. Three sites namely: Waterworks road (WR), Hill top (HT) and Azuiyiokwu (AZ) were randomly selected. Soil samples were collected from 0-15 cm and 15-30 cm depths from the sites. The soil samples were analyzed for physicochemical properties. The results showed variations in soil properties of the three sites which indicate suitability of the different sites or otherwise for waste disposal. The relationship between  percent sand  moisture content on dry mass basis and total porosity was generally highly significant (P>0.01). Similarly, the relationship between percent sand and saturated hydraulic conductivity was also highly significant. Available P highly correlated with total porosity and saturated hydraulic conductivity. The sites with high sand percentage are good for refuse disposal while the ones with high clay percentage have problems of water logging and  build up of pollution for ground water. Dumpsites Water works road (WR) and Hilltop (HT) are better for waste disposal than dumpsite Azuiyiokwu (AZ). Keywords: Dumpsites, physicochemical, refuse, soil

Compressive shock resulting from gastric distension after arterial switch operation: a case report

Compressive shock is an important cause of reversible cardiovascular compromise. Abdominal causes of compressive shock have been known to be difficult to diagnose. We report our experience in the management of compressive shock resulting from gastric distension. We consider gastric distension to be an unusual cause of compressive shock. Immediate relief of intra-abdominal tension is key in the management

Moringa oleifera Improves Skeletal Muscle Metabolism and Running Performance in Mice

Background: Recent estimates suggest that 7% of Americans use plant-derived nutritional supplements to treat a variety of complications and/or to improve athletic performance and skeletal muscle health. Unfortunately, these supplements are largely unregulated and understudied. For example, Moringa oleifera (M. oleifera) is a subtropical plant and is routinely used to treat inflammation, diabetes, obesity, cancer and HIV. However, the mechanism of action of M. oleifera has not been fully elucidated, thus the purpose of this study is to evaluate the role of M. oleifera as a novel ergogenic aid to improve exercise performance by driving peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)-dependent signaling pathways implicated in mitochondrial biogenesis and oxidative metabolism in skeletal muscle tissue. Methods: Adult male C57BL/6 mice were treated with 1.0 g of M. oleifera (N = 20) per day or vehicle control (N = 20) for a total of 5 weeks. Following 3 weeks of supplementation, half of each group (RUN) was given access to running wheels every night for 2 weeks (Remaining half = SED), distances ran were recorded daily. After treatment protocols were complete, the gastrocnemius muscles were excised and assayed for known markers of mitochondrial biogenesis, angiogenesis, endurance capacity, and capillary density using immunohistochemistry and RT-PCR. Results: Our results showed a significant increase in average distance run in the M. oleifera + SED and M. oleifera + RUN groups. This physiological trend was consistent with the molecular profile of key metabolic markers, i.e., there was an increase in levels of PGC-1α, PPARγ, SDHB, SUCLG1, VEGF, PGAM-2, PGK1, and MYLPF in the M. oleifera treated groups compared to vehicle + SED. Moreover, M. oleifera also increased CSA and decreased markers of protein degradation. Conclusions: This data suggests that M. oleifera has the potential to be an ergogenic aid via enhancing energy metabolism in adult skeletal muscle by increasing the expression of key metabolic markers, including those involved in glycolysis, oxidative phosphorylation, mitochondrial biogenesis and angiogenesis

Molecular Detection of Mycobacterium bovis in Cattle Milk in Enugu State, Nigeria

The study was carried out to detect Mycobacterium bovis in cattle milk in Enugu. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile and 21 from Monarch in Enugu. DNA was extracted from milk samples using Relia prep DNA spin column method and screened for Tuberculosis using Nested Polymerase Chain Reaction (PCR) with specific Tuberculosis primer; Insertion sequence 6110 (IS6110) while Restriction Fragment Length Polymorphism (IS6110- RFLP) method was used to differentiate between Mycobacterium bovis and Mycobacterium tuberculosis using Nar 1 digestion enzyme. Statistical tools used to analyze the data were: Chi-square, fishers’ exact test and non parametric t test. 9 (18%) samples out of the 50milk samples were positive for tuberculosis with the PCR method. 1 (2%) out of the 9 positive milk samples was found to be Mycobacterium tuberculosis while the remaining 8 (16%) were detected to be Mycobacterium bovis after using the digestion enzyme. 1 (6.3%) of the 16 milk samples collected from the Fulani settlement in 9th Mile was positive for M.bovis while a total of 2 (15.4%) out of the 13 milk samples from Gariki were positive for M.bovis and a total positive of 6 (28.6%) were detected out of the 21 milk samples analyzed from Monarch, 5 (23.8%) of which were found to be M.bovis while M. tuberculosis was detected in 1 (4.8%). The differences however, were not statistically significant (P>0.05). Keywords: Tuberculosis, Mycobacterium bovis, Mycobacterium tuberculosis, DN

Functional analysis of drug resistance-associated mutations in the Trypanosoma brucei Adenosine Transporter 1 (TbAT1) and the proposal of a structural model for the protein

The Trypanosoma brucei aminopurine transporter P2/TbAT1 has long been implicated in the transport of, and resistance to, the diamidine and melaminophenyl arsenical classes of drugs that form the backbone of the pharmacopoeia against African trypanosomiasis. Genetic alterations including deletions and single nucleotide polymorphisms (SNPs) have been observed in numerous strains and clinical isolates. Here, we systematically investigate each reported mutation and assess their effects on transporter function after expression in a tbat1 -/- T. brucei line. Out of a set of six reported SNPs from a reported ‘resistance allele’, none significantly impaired sensitivity to pentamidine, diminazene or melarsoprol, relative to the TbAT1-WT allele, although several combinations, and the deletion of the codon for residue F316, resulted in highly significant impairment. These combinations of SNPs, and ΔF316, also strongly impaired the uptake of [3H]-adenosine and [3H]-diminazene, identical to the tbat1-/- control. The TbAT1 protein model predicted that residues F19, D140 and F316 interact with the substrate of the transporter. Mutation of D140 to alanine resulted in an inactive transporter, whereas the mutation F19A produced a transporter with a slightly increased affinity for [3H]-diminazene, but reduced the uptake rate. The results presented here validate earlier hypotheses of drug binding motifs for TbAT1

Output-based assessment of herd-level freedom from infection in endemic situations:Application of a Bayesian Hidden Markov model

International audienceCountries have implemented control programmes (CPs) for cattle diseases such as bovine viral diarrhoea virus (BVDV) that are tailored to each country-specific situation. Practical methods are needed to assess the output of these CPs in terms of the confidence of freedom from infection that is achieved. As part of the STOC free project, a Bayesian Hidden Markov model was developed, called STOC free model, to estimate the probability of infection at herd-level. In the current study, the STOC free model was applied to BVDV field data in four study regions, from CPs based on ear notch samples. The aim of this study was to estimate the probability of herd-level freedom from BVDV in regions that are not (yet) free. We additionally evaluated the sensitivity of the parameter estimates and predicted probabilities of freedom to the prior distributions for the different model parameters. First, default priors were used in the model to enable comparison of model outputs between study regions. Thereafter, country-specific priors based on expert opinion or historical data were used in the model, to study the influence of the priors on the results and to obtain country-specific estimates.The STOC free model calculates a posterior value for the model parameters (e.g. herd-level test sensitivity and specificity, probability of introduction of infection) and a predicted probability of infection. The probability of freedom from infection was computed as one minus the probability of infection. For dairy herds that were considered free from infection within their own CP, the predicted probabilities of freedom were very high for all study regions ranging from 0.98 to 1.00, regardless of the use of default or country-specific priors. The priors did have more influence on two of the model parameters, herd-level sensitivity and the probability of remaining infected, due to the low prevalence and incidence of BVDV in the study regions. The advantage of STOC free model compared to scenario tree modelling, the reference method, is that actual data from the CP can be used and estimates are easily updated when new data becomes availabl