The estimation of oestriol, oestrone and oestradiol-17B in human blood

The fluorescence reaction described by Brown (1952) was reinvestigated for use with 0.05 - 0.1 jig. pure oestrogen and with blood extracts prepared in the same manner as urine extracts as described by Brown (1955). The fluorescence reaction proved satisfactory for the measurement of these small amounts of oestriol, oestrone and oestradiol in pure solution, but it was not specific enough for use with blood extracts prepared by Brown's method. Extracts which should have contained no oestrogens gave as much fluorescent activity as extracts from blood which contained large amounts of oestrogens.The Kober reaction is a highly specific reaction of the natural oestrogens, but at the time of commencement of this work was normally carried out with several microgrammes of oestrogens in a final volume of reagent of 3-4ml. The 'micro'-Kober reaction was therefore developed so that 0.05 - 1 jig. oestrogen could be measured in a final volume of 0.4 ml., optical density measurements being made in a cuvette with a light -path of 10 mm. The 'micro' - Kober reaction proved to be sufficiently sensitive and specific for use with extracts of blood obtained from the umbilical cord and from the pregnant woman. It was not sensitive enough to measure with any degree of accuracy the amount of oestrogens present in blood from non-pregnant subjects, although on a few occasions small amounts of oestrone were detected in blood from this source. Contamination with dust particles, etc. had to be rigorously excluded from the tubes during the 'micro'-Kober reaction, since this gave yellow colours which interfered in the measurement of the optical densities. The wavelength absorption curves of the colours developed in the Kober reaction indicated the presence of impurities which were derived from the benzene and benzene -ethanol used for chromatography. The impurities came in part from the quinol and in part from the benzene and the ethanol. Several attempts to purify the benzene were unsuccessful and the smallest amount of quinol necessary to give consistent readings was used. The absorption spectra of extracts of blood from pregnant women were almost identical to those of the corresponding pure oestrogen methyl ethers dissolved in the same volumes of solvents as those used for eluting them from the alumina columns, indicating that the blood extracts contained few impurities other than those derived from the solvents themselves. The absorption spectrum of 'pure' benzene and extracts from non -pregnant subjects were practically linear between 480 and 560 mμ. The use of the Allen correction formula to correct for the optical densities given by the impurities was therefore justifiable. This conclusion was further supported by the following findings. By the use of the Allen correction formula it was possible to calculate the optical densities given by the impurities. This was done for a series of standards (with and without added solvent) and for a series of blood extracts. The amounts of impurities present in the blood extracts were the same as those present in the series of pure oestrogens and solvents.The method finally adopted was almost identical to that described by Brown (1955) for the measurement of urinary oestrogens. Acid hydrolysis of whole blood proved the most satisfactory means of getting the maximum yields of oestrogens. Diluted, whole blood was hydrolysed by boiling with 15 vol. % concentrated HCl, the hydrolysed blood was extracted with ether, and the ether extracts were processed exactly as described by Brown, except that chromatography was performed with 0.7 g. alumina and the final measurement was made by the 'micro'-Kober reaction. Troublesome emulsions formed during the initial ether extractions and at partition between benzene /light petroleum and 1.6% NaOH, but these were handled by careful manipulation and centrifugation.The accuracy of the method was less than that of the urinary method. The recovery of oestrogens added after hydrolysis was 60 -7%, and the recovery of oestrogens added before hydrolysis was some 5 -20% lower. The sensitivity of the method was such that 0.18 μg. oestriol, 0.11 μg. oestrone and 0.08 μg. oestradiol could be detected in 100 ml. blood. This proved satisfactory for the measurement of oestrogens in blood from women from the 12th week of pregnancy to term.The problem of extracting oestrogens from blood was investigated. Blood was separated into plasma and red cell fractions, and in some instances the cells were washed with 0.9¡o saline. The plasma was found to contain practically all the oestrogens present in whole blood although traces of oestriol were sometimes found in the red cell fractions, particularly if the cells were not washed with saline. This is in agreement with the work of Wall and Migeon (1959) who showed that successive saline or plasma washes removed all the oestrogen from the red cell fraction, which they found to contain up to 20% of the total oestrogen content.Direct ether extraction of whole blood, or extraction with ethanol -ether or ethanol removed 10 -20% of the total oestriol and 80 -90% of the oestrone and oestradiol (yield given by boiling with 15 vol. % HCl being taken as 100%). The ethanol or ethanol -ether fraction yielded a further 30-40% of the oestriol on hydrolysis by boiling with HC1 or by incubating with ß- glucuronidase. The remainder of the oestriol was recovered from the protein precipitate by acid hydrolysis followed by ether extraction. Hydrolysis of the whole blood by boiling with HCl in concentrations varying from 2.5 to 20 vol.% gave the highest yields of 'oestrogens, the acid concentration not being critical. There was no increase in the amount of oestriol which was extractable with ether after incubation of the blood with ß-glucuronidase. These experiments indicate that almost all of the oestrone and oestradiol are in the free form in blood, 10-20% of the oestriol is in the free form, 30-40% is either protein-bound or conjugated or possibly both protein-bound and conjugated, and the remainder is more firmly protein- bound.Blood oestrogen levels were measured in normal, healthy, pregnant women, patients who were delivered by Caesarean section and patients suffering from pre-eclampsia. There were no differences in the levels in primigravid and parous subjects. There was a very wide scatter of results in the normal patients studied. Since there were no significant diurnal or daily variations in the concentration of blood oestrogens, as judged by the results obtained at various times of the day, and on consecutive days in four patients studied in the last trimester of pregnancy, this scatter was due to real differences in the individual levels. The concentrations of oestrogens rose from the 12th week of pregnancy to term. This rise was usually continuous, but in some patients the levels sometimes fell temporarily, usually to rise again. The three oestrogens rose at about the same rate until the 32nd week of pregnancy when the oestriol began to increase more rapidly than the oestrone and oestradiol. These rises in blood oestrogen levels closely paralleled the corresponding rises in urinary oestrogen output. Using the mean values in Series A and the figures for urinary output (Brown, 1956) of 5,100; 510 and 140 μg. oestriol, oestrone and oestradiol at 20 weeks, and 33,500; 1,550 and 570 μg. at 40 weeks, the factors for the increases in oestrogen concentrations between the 20th and 40th week were (urine /blood) 6.5/6.0, 3.0/3.3 and 4.1/4.4 for oestriol, oestrone and oestradiol respectively. These calculations showed that the increases in the concentrations of all three oestrogens in urine closely paralleled the increases in blood, even to the extent of the more rapid increase in oestriol concentration after the 32nd week. Although the increases in blood and urine followed one another closely, the ratio of the relative concentrations of oestriol, oestrone and oestradiol were markedly different for blood and urine. Using the same values as in the above calculations, the ratio of oestriol: oestrone: oestradiol were 22:1:0.37 and 1.8 :1:0.22 for blood and urine respectively. The difference in ratio of oestriol to oestrone suggested that oestriol is cleared very much more rapidly than oestrone, but, on the other hand it might be the results of the difference in the relative proportions of free, conjugated and "protein- bound" oestriol and oestrone in blood.Since the blood levels showed no significant diurnal variation, and the ratio of blood concentration to urinary oestrogen output per hour remained more or less constant, it appears that the rate of excretion of oestrogens must also remain constant. These ratios were used as a basis for speculation on the levels one might expect to find in blood from non -pregnant subjects, and on possible modes of excretion of the oestrogens.The concentration of oestrogens in amniotic fluid, umbilical venous and arterial blood and uterine venous and arm venous blood of the mother at Caesarean section were measured. Liquor contained very large amounts of conjugated oestriol and only 4% of free oestriol; in other words its oestrogen content resembled that of urine rather than that of blood. There was no significant difference in the concentration of the three oestrogens in umbilical venous and arterial blood. Small differences might have been expected if the foetus were an important site for the metabolism of oestrone and oestradiol (Diczfalusy and Magnusson, 1958). The concentrations in maternal uterine venous blood were significantly higher than those in the maternal peripheral blood, sometimes by as much as 25%. This difference probably represents the gradient caused by entry of oestrogens into the maternal circulation from the placenta and loss through maternal metabolism and renal and faecal excretion of the oestrogens. The fact that a difference is detectable with the high uterine blood flow of approximately 600 ml/minute indicates a considerable production of oestrogens by the placenta. The peripheral oestrone levels were significantly lower in the women undergoing Caesarean section than in the normal series. This difference was shown not to be due to any premedication administered to the patients, and the possibility that it is due to rest in bed is under investigation. In support of this possibility it is interesting to note that the blood oestrone levels in a series of patients admitted to hospital with a variety of minor ailments were also significantly lower than those of the normal out-patients and that the urinary output of oestrogens falls overnight in pregnant and non-pregnant subjects (Brown, unpublished observations); similarly Fotherby and Strong (1960) showed that the urinary output of the metabolites of a number of adrenal steroids fell between 9 p.m. and 6.30 a.m.In the majority of cases of pre-eclampsia, there was a decrease in the concentration of oestriol and oestrone in blood, but little or no change in the oestradiol concentration, except in some cases of intra-uterine death. In all cases of still-birth the oestrone concentration was below the normal minimum and in almost all these cases the oestriol concentration was also below the normal minimum. These low concentrations of blood oestrogens were presumably due to placental dysfunction. In a few cases of severe pre -eclampsia where the symptoms were of short duration and had appeared after the 34th week of pregnancy, the oestriol concentration was above the normal mean value and in one case was higher than the maximum value observed in the normal series, although the oestrone values were low. It was suggested that these higher levels might be the result of the renal damage which is known to occur in pre-eclampsia.The results obtained by using the present method agreed well with those obtained by Preedy and co- workers and Oertel et al, while the oestrone values of Svendsen were lower, but again there was good agreement in the oestradiol values. There was very close agreement between the results for cord blood quoted here and those of Diczfalusy and Magnusson. The specificity of these methods and the fluorimetric methods of Veldhuis and Varangot et al were discussed.Very small amounts of oestriol and oestrone were found in samples of blood taken from non-pregnant subjects, and the mean values were below the limits of sensitivity of the method. Preliminary observations using the more sensitive fluorimetric method of Ittrich (1958) suggest that the levels are even lower than those indicated by the results of the 'micro'-Kober reaction and are near the limits of sensitivity of the Ittrich reaction

Predictors of activity engagement during pre-school physical education

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An Integrated Single Vendor-Buyer Stochastic Inventory Model with Partial Backordering under Imperfect Production and Carbon Emissions

This paper develops an integrated single vendor single buyer inventory model with imperfect quality and environmental impact. The demand during lead time is assumed to be stochastic and follows the normal distribution. An integrated system with controllable lead time and logarithmic investment to reduce the defective percentage is discussed in this model.100% error-free screening process is adopted by the buyer to separate defective and non-defective items. We assume that shortages are allowed and are partially backordered at the buyer’s end. Logistics management is the component of supply chain management that focusses on how and when to get raw materials, intermediate products and finished goods from their respective origins to their destinations.Thus, transportation play a major role in supply chain. As transportation increases, it affects the weather by the matter of carbon emission.The fixed and variable carbon emission cost for both vendor and buyer is considered. The prime motive is to determine the optimal policies regarding optimal order quantity, reorder point, lead time and the number of lots delivered in a production run by minimizing the expected total cost of the system. Finally, a numerical example is provided to demonstrate the model

Analysis of cost estimation using the web metrics and cost driver in the high performance of web developers

In recent years, many researchers and software industries have given significant attention to the estimation of software effort. Software effort estimation is a challenge that often appears in the project of making software. To address this issue, various software expansion cost estimations have been familiarized. A basic traditional method for cost estimation is the Constructive Cost Model II (COCOMO II Model), which generates huge gradations of substantiality. The proposed COCOMO II model employs cost drivers, measures and the streak of programmes to predict the energy and the growth period of a web rise. However, the model is still lacking in terms of accuracy in effort and development time estimation. In this study, we do investigate the influence of components and attributes to achieve new better accuracy improvement on the COCOMO II model. The NASA93 dataset is used to test the suggested methodology. The system has outperformed earlier investigations and arrangements capable of imperfect data input, and further advancements in the reliability of the estimated technique

Keberhasilan Asimilasi Dalam Merubah Karakter Narapidana Di Balai Pemasyarakatan Kelas Dua Manado

Penelitian ini bertujuan untuk memberikan gambaran umum dan khusus tentang keberhasilan proses asimilasi, mereintegrasi narapidana dalam masyarakat. Menjadi lokasi penelitian ini adalah Kantor Balai Pemasyarakatan Kelas II Manado yang terletak di Jalan Pumorow nomor 106, Kelurahan Teling, Manado Utara. Dalam pengumpulan data, penulis menggunakan teknik observasi dan wawancara, adapun jumlah informan dalam penelitian ini, ada 7 informan. Hasil penelitian ini membuktikan bahwa pelaksanaaan proses asimilasi sisi efisiensi sudah berhasil, namun dari sisi penerapan secara merata ke narapidana masih perlu dimaksimalkan. Karena masih ada narapidana yang berhak mengikuti proses asimilasi, namun terhalang oleh indikasi latar belakang berbuatan yang di nilai beresiko terhadap masyarakat sekitar, contohnya; penipuan dan pencurian

Effect of Relative Marker Movement on the Calculation of the Foot Torsion Axis Using a Combined Cardan Angle and Helical Axis Approach

The two main movements occurring between the forefoot and rearfoot segment of a human foot are flexion at the metatarsophalangeal joints and torsion in the midfoot. The location of the torsion axis within the foot is currently unknown. The purpose of this study was to develop a method based on Cardan angles and the finite helical axis approach to calculate the torsion axis without the effect of flexion. As the finite helical axis method is susceptible to error due to noise with small helical rotations, a minimal amount of rotation was defined in order to accurately determine the torsion axis location. Using simulation, the location of the axis based on data containing noise was compared to the axis location of data without noise with a one-sample t-test and Fisher's combined probability score. When using only data with helical rotation of seven degrees or more, the location of the torsion axis based on the data with noise was within 0.2 mm of the reference location. Therefore, the proposed method allowed an accurate calculation of the foot torsion axis location