Occurrence of the alien nudibranch Melibe viridis (Kelaart, 1858) (Opisthobranchia, Tethydidae), in the Maltese Islands

The alien dendronotacean nudibranch Melibe viridis (Kelaart, 1858), a tropical Indo-Pacific species that seems to have been introduced by shipping into the Mediterranean via the Suez Canal, and which has established populations in Greece, Turkey, Cyprus, Montenegro, Croatia, NW Sicily, southern peninsular Italy and Djerba Island in the Gulf of Gabes, is recorded for the first time from Malta. A thriving population was observed on a soft sediment bottom at a depth of 18-20 m off the western coast of the island of Comino (Maltese Islands). It is suggested that this species was introduced into Malta due to a natural range expansion of surrounding populations

Application of SCOPE-C to measure social inclusion among mental health services users in Hong Kong

This study describes the construction of the Chinese version of the Social and Communities Opportunities Profile (SCOPE), henceforth, the SCOPE-C, to measure social inclusion among mental health services users in Hong Kong. The SCOPE-C was developed based on concept-mapping and benchmarking of census questions. The questionnaire consisted of 56 items, went through a standardized linguistic validation process and was pilot tested with qualitative feedback from five users of mental health services. Altogether 168 Chinese service users were recruited through various NGO mental health services to have three times face-to-face interview between October 2013 and July 2014. Results indicated that items related to satisfaction with opportunities and perceived opportunities in various social domains had high consistency. Nearly all the Kappa statistics and Pearson correlation coefficients between the baseline and two rounds of re-test were significant. The SCOPE-C was considered a valid instrument for Hong Kong mental health user population

Finite element analysis and computer aided tissue engineering design of a replacement lumbar intervertebral disc

Paper presented at the 2006 IEEE 32nd Annual Northeast Bioengineering Conference, Easton, PA.The treatment of disc degeneration disease of the spine has been a subject of particular interest in the medical community due to its effects on the lifestyle of afflicted patients. Current treatment modalities range from non-invasive treatment with physical therapy, to the invasive surgical repair of the degenerated disc(s). However, despite the existence of these treatment methods, each has its own set of drawbacks and limitations, most notably the use of surgical intervention. Examples of such limitations have included the mechanical failure of spinal implants, the destruction of the vertebral bone structure due to implant subsistence, graft site morbidity due to bone harvesting, and the promotion of disc degeneration at the surrounding spinal units. To overcome these limitations, a new implant design was conceived combining the concepts of arthrodesis, arthroplasty, and fusion via bone graft, allowing for the biological fusion of adjacent vertebra, in conjunction with the use of tissue engineering principals. In addition, this new approach to implant development, utilizing the practices of computer aided tissue engineering, permits the patient specific design of the implant, enhancing the ability to match implant design and architecture with patient anatomy. Resulting from these design criteria, the proposed novel design eliminates the drawbacks associated with the current vertebral implant designs, potentially extending both the lifespan and effectiveness of the implant, thereby improving the long term outcomes for the treatment of spinal disc degeneration

Illuminating the bacterial microbiome of Australian ticks with 16S and Rickettsia-specific next-generation sequencing

Next-generation sequencing (NGS) studies show that mosquito and tick microbiomes influence the transmission of pathogens, opening new avenues for vector-borne pathogen control. Recent microbiological studies of Australian ticks highlight fundamental knowledge gaps of tick-borne agents. This investigation explored the composition, diversity and prevalence of bacteria in Australian ticks (n = 655) from companion animals (dogs, cats and horses). Bacterial 16S NGS was used to identify most bacterial taxa and a Rickettsia-specific NGS assay was developed to identify Rickettsia species that were indistinguishable at the V1-2 regions of 16S. Sanger sequencing of near full-length 16S was used to confirm whether species detected by 16S NGS were novel. The haemotropic bacterial pathogens Anaplasma platys, Bartonella clarridgeiae, “Candidatus Mycoplasma haematoparvum” and Coxiella burnetii were identified in Rhipicephalus sanguineus (s.l.) from Queensland (QLD), Western Australia, the Northern Territory (NT), and South Australia, Ixodes holocyclus from QLD, Rh. sanguineus (s.l.) from the NT, and I. holocyclus from QLD, respectively. Analysis of the control data showed that cross-talk compromises the detection of rare species as filtering thresholds for less abundant sequences had to be applied to mitigate false positives. A comparison of the taxonomic assignments made with 16S sequence databases revealed inconsistencies. The Rickettsia-specific citrate synthase gene NGS assay enabled the identification of Rickettsia co-infections with potentially novel species and genotypes most similar (97.9–99.1%) to Rickettsia raoultii and Rickettsia gravesii. “Candidatus Rickettsia jingxinensis” was identified for the first time in Australia. Phylogenetic analysis of near full-length 16S sequences confirmed a novel Coxiellaceae genus and species, two novel Francisella species, and two novel Francisella genotypes. Cross-talk raises concerns for the MiSeq platform as a diagnostic tool for clinical samples. This study provides recommendations for adjustments to Illuminaʼs 16S metagenomic sequencing protocol that help track and reduce cross-talk from cross-contamination during library preparation. The inconsistencies in taxonomic assignment emphasise the need for curated and quality-checked sequence databases

Percolation and jamming in random sequential adsorption of linear segments on square lattice

We present the results of study of random sequential adsorption of linear segments (needles) on sites of a square lattice. We show that the percolation threshold is a nonmonotonic function of the length of the adsorbed needle, showing a minimum for a certain length of the needles, while the jamming threshold decreases to a constant with a power law. The ratio of the two thresholds is also nonmonotonic and it remains constant only in a restricted range of the needles length. We determine the values of the correlation length exponent for percolation, jamming and their ratio

Singular Casimir Elements of the Euler Equation and Equilibrium Points

The problem of the nonequivalence of the sets of equilibrium points and energy-Casimir extremal points, which occurs in the noncanonical Hamiltonian formulation of equations describing ideal fluid and plasma dynamics, is addressed in the context of the Euler equation for an incompressible inviscid fluid. The problem is traced to a Casimir deficit, where Casimir elements constitute the center of the Lie-Poisson algebra underlying the Hamiltonian formulation, and this leads to a study of the symplectic operator defining the Poisson bracket. The kernel of the symplectic operator, for this typical example of an infinite-dimensional Hamiltonian system for media in terms of Eulerian variables, is analyzed. For two-dimensional flows, a rigorously solvable system is formulated. The nonlinearity of the Euler equation makes the symplectic operator inhomogeneous on phase space (the function space of the state variable), and it is seen that this creates a singularity where the nullity of the symplectic operator (the "dimension" of the center) changes. Singular Casimir elements stemming from this singularity are unearthed using a generalization of the functional derivative that occurs in the Poisson bracket

Jamming coverage in competitive random sequential adsorption of binary mixture

We propose a generalized car parking problem where cars of two different sizes are sequentially parked on a line with a given probability $q$. The free parameter $q$ interpolates between the classical car parking problem of only one car size and the competitive random sequential adsorption (CRSA) of a binary mixture. We give an exact solution to the CRSA rate equations and find that the final coverage, the jamming limit, of the line is always larger for a binary mixture than for the uni-sized case. The analytical results are in good agreement with our direct numerical simulations of the problem.Comment: 4 pages 2-column RevTeX, Four figures, (there was an error in the previous version. We replaced it (including figures) with corrected and improved version that lead to new results and conclusions

Mineral resource information in support of national, regional and local planning : Suffolk

This report is one of a series prepared by the British Geological Survey for various administrative areas in England for the Office of the Deputy Prime Minister's research project Mineral Resource Information in Support of National, Regional and Local Planning. The accompanying map relates to the county of Suffolk and delineates the mineral resources of current, or potential, economic interest in the area and the sites where minerals are or have been worked. It also relates these to national planning designations, which may represent constraints on the extraction of minerals. Three major elements of information are presented: • the geological distribution and importance of mineral resources; • the extent of mineral planning permissions and the location of current mineral workings, and • the extent of selected, nationally-designated planning constraints. This wide range of information, much of which is scattered and not always available in a consistent and convenient form, is presented on a digitally-generated summary map on the scale of 1:100 000. This scale is convenient for the overall display of the data and allows for a legible topographic base on which to depict the information. However, all the data are held digitally at larger scales using a Geographical Information System (GIS), which allows easy revision, updating and customisation of the information together with its possible integration with other datasets. The information will form part of a Summary of the Mineral Resources of the East of England Region. The purpose of the work is to assist all interested parties involved in the preparation and review of development plans, both in relation to the extraction of minerals and the protection of mineral resources from sterilisation. It provides a knowledge base, in a consistent format, on the nature and extent of mineral resources and the environmental constraints, which may affect their extraction. An important objective is to provide baseline data for the long term. The results may also provide a starting point for discussions on specific planning proposals for mineral extraction or on proposals, which may sterilise resources. It is anticipated that the maps and report will also provide valuable background data for a much wider audience, including the different sectors of the minerals industry, other agencies and authorities (e.g. The Planning Inspectorate Agency, the Environment Agency, the Countryside Agency and English Nature), environmental interests and the general public. Basic mineral resource information is essential to support mineral exploration and development activities, for resource management and land-use planning, and to establish baseline data for environmental impact studies and environmental guidelines. It also enables a more sustainable pattern and standard of development to be achieved by valuing mineral resources as national assets. The mineral resources covered are sand and gravel, brick clay, hydrocarbons, building stone, chalk and peat

Determining patterns in the composition of dissolved organic matter in fresh waters according to land use and management

In fresh waters, the origins of dissolved organic matter (DOM) have been found to exert a fundamental control on its reactivity, and ultimately, its ecosystem functional role. A detailed understanding of landscape scale factors that control the export of DOM to aquatic ecosystems is, therefore, pivotal if the effects of DOM flux to fresh waters are to be fully understood. In this study we present data from a national sampling campaign across the United Kingdom in which we explore the variability in DOM composition in three broad landscape types defined by similar precipitation, geology, land use and management, hydrology, and nutrient enrichment status. We characterised samples from fifty-one sites, grouping them into one of three major underlying classifications: circumneutral streams underlain by clay and mudstone (referred to as ‘clay’), alkaline streams underlain by Cretaceous Chalk or by Carboniferous or Jurassic Limestone (‘limestone’), and acidic streams in peatland catchments underlain by a range of low permeability lithologies (‘peat’). DOM composition was assessed through organic matter stoichiometry (organic carbon: organic nitrogen; organic carbon: organic phosphorus; C/N(P)DOM) and metrics derived from ultra-violet (UV)/visible spectroscopic analysis of DOM such as specific UV absorption (a254 nm; SUVA254). We found similar SUVA254, C/NDOM and DOM/a254 relationships within classifications, demonstrating that despite a large degree of heterogeneity within environments, catchments with shared environmental character and anthropogenic disturbance export DOM with a similar composition and character. Improving our understanding of DOM characterisation is important to help predict shifts in stream ecosystem function, and ecological responses to enrichment or mitigation efforts and how these may result in species composition shifts and biodiversity loss in freshwater ecosystems