Percepción sobre habilidades investigativas de las estudiantes de segunda especialidad de enfermeria en docentes de La Universidad Privada Antenor Orrego, Trujillo-2022

El estudio denominado Percepción sobre habilidades investigativas de las estudiantes de segunda especialidad de enfermería en docentes de la Universidad Privada Antenor Orrego, Trujillo-2020, cuyo propósito fue comprender las significaciones que le atribuyen los docentes respecto a las habilidades investigativas de los estudiantes de segunda especialidad en enfermería, como respuesta una problemática sentida en la formación de profesionales y especialistas en enfermería. Investigación de tipo descriptiva cualitativa orientada a la comprensión de hechos o vivencias en contextos académicos que involucra a estudiantes y docentes de la segunda especialidad de la Universidad Antenor Orrego en situaciones vivenciales de formación y en los cursos formativos de especialización, desde la perspectiva de la comprensión subjetiva de los hechos y comportamientos del estudiante en su proceso de enfrentar al estudio con manejo de la investigación científica. Los resultados obtenidos producto de las significaciones de los docentes sobre las habilidades investigativas que muestran los estudiantes de especialización en enfermería, se comprende que existe mucha dificultad en el manejo de los procesos de la investigación desde la problematización hasta la comprobación de la realidad mediante o de los hechos a partir de la triangulación de informantes y aporte teórico de los fundamentos, enfoques y teorías que dan soporte a la investigación y también constituye soporte teórico metodológico que garantiza la credibilidad de losresultados. El aporte de la fenomenología hacia la educación es entendido como un proceso de formación del estudiante desde una perspectiva holística o globalizante y con el uso de la hermenéutica como un razonamiento subjetivo permitió, explorar, valorar y evaluar sus interacciones, vivencias y emociones para la comprensión de los sujetosTesi

Cuidado de la salud renal en el primer nivel de atención

Objetivo: Proponer intervenciones para el cuidado de la salud renal en el primer nivel de atención. Metodología: Se realizó un análisis documental y búsqueda bibliográfica de los beneficios de la prevención e identificación de daño renal temprano. Resultados: Según la revisión de investigaciones, se demuestra que las actividades de los programas de salud renal aportarían a la prevención de la enfermedad, que esta no llegue a ser degenerativa y/o crónica. Conclusiones: La implementación de un programa de salud renal en el primer nivel de atención permitiría realizar detección precoz de injuria renal, brindando tratamiento oportuno y referencia adecuada. Además, el programa permitirá dar énfasis en cambios de estilo de vida y hábitos alimenticios con enfermedades de riesgo que conlleva a daño renal

Receptores dopaminergicos en la enfermedad de parkinson y otras enfermedades de los ganglios

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura 04-06-198

Ultrasmall, water dispersible, TWEEN80 modified Yb:Er:NaGd(WO4)2 nanoparticles with record upconversion ratiometric thermal sensitivity and their internalization by mesenchymal stem cells

This work presents the synthesis by coprecipitation of diamond shaped Yb:Er:NaGd(WO) crystalline nanoparticles (NPs) with diagonal dimensions in the 5-7 nm ×10-12 nm range which have been modified with TWEEN80 for their dispersion in water, and their interaction with mesenchymal stem cells (MSCs) proposed as cellular NP vehicles. These NPs belong to a large family of tetragonal Yb:Er:NaT(XO) (T = Y, La, Gd, Lu; X = Mo, W) compounds with green (H + S → I) Er-related upconversion (UC) efficiency comparable to that of Yb:Er:β-NaYF reference compound, but with a ratiometric thermal sensitivity (S) 2.5-3.5 times larger than that of the fluoride. At the temperature range of interest for biomedical applications (∼293-317 K/20-44 °C) S = 108-118 ×10 K for 20 at%Yb:5 at%Er:NaGd(WO) NPs, being the largest values so far reported using the H/S Er intensity ratiometric method. Cultured MSCs, incubated with these water NP emulsions, internalize and accumulate the NPs enclosed in endosomes/lysosomes. Incubations with up to 10 μg of NPs per ml of culture medium maintain cellular metabolism at 72 h. A thermal assisted excitation path is discussed as responsible for the UC behavior of Yb:Er:NaT(XO) compounds.Work supported by the Spanish Ministry of Economy, Industry and Competitiveness and by the European Regional Development Fund trough MAT2014-56607-R project

Liver Growth Factor “LGF” as a Therapeutic Agent for Alzheimer’s Disease

Alzheimer’s disease (AD) is a progressive degenerative disorder and the most common cause of dementia in aging populations. Although the pathological hallmarks of AD are well defined, currently no effective therapy exists. Liver growth factor (LGF) is a hepatic albumin–bilirubin complex with activity as a tissue regenerating factor in several neurodegenerative disorders such as Parkinson’s disease and Friedreich’s ataxia. Our aim here was to analyze the potential therapeutic effect of LGF on the APPswe mouse model of AD. Twenty-month-old mice received intraperitoneal (i.p.) injections of 1.6 µg LGF or saline, twice a week during three weeks. Mice were sacrificed one week later, and the hippocampus and dorsal cortex were prepared for immunohistochemical and biochemical studies. LGF treatment reduced amyloid-β (Aβ) content, phospho-Tau/Tau ratio and the number of Aβ plaques with diameter larger than 25 µm. LGF administration also modulated protein ubiquitination and HSP70 protein levels, reduced glial reactivity and inflammation, and the expression of the pro-apoptotic protein Bax. Because the administration of this factor also restored cognitive damage in APPswe mice, we propose LGF as a novel therapeutic tool that may be useful for the treatment of AD.R.G.-G. was the recipient of a Research Supporting Staff Grant Contract (Spanish Health Research Fund (FIS)).Peer reviewe

Immunohistochemical Study of ASC Expression and Distribution in the Hippocampus of an Aged Murine Model of Alzheimer’s Disease

Neuroinflammation is involved in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease (AD), and is notably dependent on age. One important inflammatory pathway exerted by innate immune cells of the nervous system in response to danger signals is mediated by inflammasomes (IF) and leads to the generation of potent pro-inflammatory cytokines. The protein “apoptosis-associated speck-like protein containing a caspase recruitment domain” (ASC) modulates IF activation but has also other functions which are crucial in AD. We intended to characterize immunohistochemically ASC and pattern recognition receptors (PRR) of IF in the hippocampus (HP) of the transgenic mouse model Tg2576 (APP), in which amyloid-beta (Aβ) pathology is directly dependent on age. We show in old-aged APP a significant amount of ASC in microglia and astrocytes associated withAβ plaques, in the absence of PRR described by others in glial cells. In addition, APP developed foci with clusters of extracellular ASC granules not spatiallyrelated to Aβ plaques, which density correlated with the advanced age of mice and AD development. Clusters were associated withspecific astrocytes characterized by their enlarged ring-shaped process terminals, ASC content, and frequent perivascular location. Their possible implication in ASC clearance and propagation of inflammation is discussed

Temporal Profile of Levels of Monoamines and Their Metabolites in Striata of Rats Implanted with Dialysis Tubes

4 páginas.We have measured the levels of monoamines and their metabolites in rat striata implanted with a dialysis tube, in contralateral nonimplanted striata, and in diialysates obtained from the dialysis tube. The perfusion was done with Ringer solution. The animals were perfused either for a continuous period of 7 h at 1 day after implantation or for periods of 2 h on days 1, 4, and 7 after implantation] In animals perfused for 7 h, levels of monoamine metabolites in dialy-sates remained stable for the first 4 h of perfusion, but a reduction was observed during the last 3 h. In Animals perfused for 2 h on days 1, 4, and 7 after implantation, we observed a progressive reduction in levels of metabolites in di-alysates with respect to the first day of perfusion The levels of dopamine and its metabolites in the striata in which a dialysis cannula had been implanted showed a progressive reduction during the period postimplantation comparable to that observed in dialysates. The levels of 3-methoxytyramine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid were elevated 24 h after implantation in the implanted striata with respect to the contralateral nonimplanted striata, but 7 days after implantation, the levels of dopamine were decreased in the implanted striata, and tne levels of metabolites were unchanged.Peer reviewe

Effects of Intravenous Administration of Human Umbilical Cord Blood Stem Cells in 3-Acetylpyridine-Lesioned Rats

Cerebellar ataxias include a heterogeneous group of infrequent diseases characterized by lack of motor coordination caused by disturbances in the cerebellum and its associated circuits. Current therapies are based on the use of drugs that correct some of the molecular processes involved in their pathogenesis. Although these treatments yielded promising results, there is not yet an effective therapy for these diseases. Cell replacement strategies using human umbilical cord blood mononuclear cells (HuUCBMCs) have emerged as a promising approach for restoration of function in neurodegenerative diseases. The aim of this work was to investigate the potential therapeutic activity of HuUCBMCs in the 3-acetylpyridine (3-AP) rat model of cerebellar ataxia. Intravenous administered HuUCBMCs reached the cerebellum and brain stem of 3-AP ataxic rats. Grafted cells reduced 3-AP-induced neuronal loss promoted the activation of microglia in the brain stem, and prevented the overexpression of GFAP elicited by 3-AP in the cerebellum. In addition, HuUCBMCs upregulated the expression of proteins that are critical for cell survival, such as phospho-Akt and Bcl-2, in the cerebellum and brain stem of 3-AP ataxic rats. As all these effects were accompanied by a temporal but significant improvement in motor coordination, HuUCBMCs grafts can be considered as an effective cell replacement therapy for cerebellar disorders