«Nos queremos gordas»: un reflejo vivencial de las opresiones gordofóbicas y cómo combatirlas.

Este trabajo tiene como finalidad visibilizar los distintos tipos de violencia a las que están expuestas las mujeres gordas en su vida cotidiana. De esta manera, y, a través de las experiencias y vivencias personales y compartidas, se busca mostrar las diferentesrealidades y situaciones en las que se ejerce la violencia estructural hacia las personas gordas llamada: gordofobia. A lo largo del trabajo se muestra que la gordofobia no es solo una cuestión de rechazo individual, sino que va más allá y está presente en todos los espacios a los que accedemos las personas. Se muestra la gordofobia estética con la presencia de un canon que nos excluye y nos oprime a través de la falta de prendas con las que podamos vestirnos, una gordofobia verbal y material utilizada para elaborar un discurso de odio y culpa y, una violencia médica que nos impide recibir un diagnóstico apropiado y, que, en ocasiones, nos mata. Para luchar contra la opresión, nace con fuerza el activismo gordo y nos enseña que nuestros cuerpos son válidos y merecen el mismo respeto y los mismos cuidados que las corporalidades normativas. Porque la opresión existe y seguirá existiendo, pero hemos creado comunidad y ya no estamos solas para enfrentarnos a ella. <br /

Combinatorial formulas for Kazhdan-Lusztig polynomials with respect to W-graph ideals

In \cite{y1} Yin generalized the definition of $W$-graph ideal $E_J$ in weighted Coxeter groups and introduced the weighted Kazhdan-Lusztig polynomials $\left \{ P_{x,y} \mid x,y\in E_J\right \}$, where $J$ is a subset of simple generators $S$. In this paper, we study the combinatorial formulas for those polynomials, which extend the results of Deodhar \cite{v3} and Tagawa \cite{h1}.Comment: 16 page

Innovación Educativa en la Enseñanza del Diseño de Fármacos en el Grado y en el Máster de Farmacia

Memoria ID11-159. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2011-2012

Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica : Identification of genes and pathways involved in conferring immunoprotection in a murine model

Background: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F. hepatica. Methods: The mice were immunised with synthetic peptides containing B- and T-cell epitopes, which are derived from F. hepatica cathepsin B and amoebapore proteins, as novel vaccine candidates against F. hepatica formulated in an adjuvant adaptation vaccination system; they were experimentally challenged with F. hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. Data was then background corrected, normalised and the expression signal was calculated. The Ingenuity Pathway Analysis tool was then used for analysing differentially expressed gene identifiers for annotating bio-functions and constructing and visualising molecular interaction networks. Results: Mice immunised with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes, 168 genes being up-regulated and 652 genes being down-regulated, p value &lt;0.05, fold change ranging from -2.944 to 7.632. A functional study of these genes revealed changes in the pathways related to nitric oxide and reactive oxygen species production, Interleukin-12 signalling and production in macrophages and Interleukin-8 signalling with up-regulation of S100 calcium-binding protein A8, Matrix metallopeptidase 9 and CXC chemokine receptor 2 genes. Conclusion: The data obtained in the present study provided us with a more comprehensive overview concerning the possible molecular pathways implied in inducing protection against F. hepatica in a murine model, which could be useful for evaluating future vaccine candidates. © 2017 The Author(s)

Neoflavonoids as Inhibitors of HIV-1 Replication by Targeting the Tat and NF-kB Pathways

Twenty-eight neoflavonoids have been prepared and evaluated in vitro against HIV-1. Antiviral activity was assessed on MT-2 cells infected with viral clones carrying the luciferase reporter gene. Inhibition of HIV transcription and Tat function were tested on cells stably transfected with the HIV-LTR and Tat protein. Seven 4-phenylchromen-2-one derivatives showed HIV transcriptional inhibitory activity but only the phenylchrome-2-one 10 inhibited NF- B and displayed anti-Tat activity simultaneously. Compounds 10, 14, and 25, inhibited HIV replication in both targets at concentrations <25 M. The assays of these synthetic 4-phenylchromen-2-ones may aid in the investigation of some aspects of the anti-HIV activity of such compounds and could serve as a scaffold for designing better anti-HIV compounds, which may lead to a potential anti-HIV therapeutic dru

Peptides Derived of Kunitz-Type Serine Protease Inhibitor as Potential Vaccine Against Experimental Schistosomiasis

Schistosomiasis is a significant public health problem in sub-Saharan Africa, China, Southeast Asia, and regions of South and Central America affecting about 189 million people. Kunitz-type serine protease inhibitors have been identified as important players in the interaction of other flatworm parasites with their mammalian hosts. They are involved in host blood coagulation, fibrinolysis, inflammation, and ion channel blocking, all of them critical biological processes, which make them interesting targets to develop a vaccine. Here, we evaluate the protective efficacy of chemically synthesized T- and B-cell peptide epitopes derived from a kunitz protein from Schistosoma mansoni. Putative kunitz-type protease inhibitor proteins were identified in the S. mansoni genome, and their expression was analyzed by RNA-seq. Gene expression analyses showed that the kunitz protein Smp_147730 (Syn. Smp_311670) was dramatically and significantly up-regulated in schistosomula and adult worms when compared to the invading cercariae. T- and B-cell epitopes were predicted using bioinformatics tools, chemically synthesized, and formulated in the Adjuvant Adaptation (ADAD) vaccination system. BALB/c mice were vaccinated and challenged with S. mansoni cercariae. Kunitz peptides were highly protective in vaccinated BALB/c mice showing significant reductions in recovery of adult females (89–91%) and in the numbers of eggs trapped in the livers (77–81%) and guts (57–77%) of mice. Moreover, liver lesions were significantly reduced in vaccinated mice (64–65%) compared to infected control mice. The vaccination regime was well-tolerated with both peptides. We propose the use of these peptides, alone or in combination, as reliable candidates for vaccination against schistosomiasis. © Copyright © 2019 Hernández-Goenaga, López-Abán, Protasio, Vicente Santiago, del Olmo, Vanegas, Fernández-Soto, Patarroyo and Muro

Máster de Enfermedades Tropicales en las Redes Sociales: creación y uso de materiales audiovisuales, gestión de contenidos y difusión dentro del EEES.

Memoria ID-255. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014

Intervención socio - familiar para la reinserción social de hombres cuyo consumo principal es la cocaína.

Las drogodependencias son uno de los principales problemas que más preocupa a la sociedad, por sus consecuencias en el plano social, personal, familiar, sanitario, laboral y económico del individuo. La familia puede verse afectada por la drogodependencia de uno de sus miembros provocando alteraciones en el sistema familiar. Por este motivo, poder trabajar con la familia del adicto es una herramienta de apoyo muy importante a lo largo de todo el proceso de intervención. Para abordar este tipo de problemática es necesaria una intervención multidimensional en los diferentes aspectos de la vida personal, debido a que afecta tanto a la vida de la persona como al entorno directo e inmediato ocasionando una serie de conflictos que conducen a una inminente y progresiva exclusión social

Actividad analgésica y antiinflamatoria de derivados de podofilotoxina

La podofilotoxina es un producto natural inhibidor de la polimerización de la tubulina, que ha servido de prototipo para el desarrollo de distintos agentes antitumorales, actualmente en uso clínico como etopósido, tenipósido y etopophos. Reumacon, otro derivado semisintético similar a los anteriores, llegó a fase clínica para el tratamiento de la artritis reumatoide. Durante un estudio fitoquímico llevado a cabo sobre las hojas de Juniperus thurifera L., se aislaron, entre otros productos, varios ciclolignanos, podofilotoxina, desoxipodofilotoxina, desoxipicropodofilina y ácido thuriférico. Estos compuestos, obtenidos posteriormente mediante semisíntesis, fueron ensayados como analgésicos y antiinflamatorios. Desoxipodofilotoxina, una sustancia conformacionalmente rígida debido a la trans-lactona tensionada, muestra una elevada citotoxicidad; su epímero en C-8’, desoxipicropodofilina, con una cis-lactona más flexible y cien veces menos citotóxica, muestra un potente efecto analgésico, aunque menor actividad antiinflamatoria

Benzimidazole and aminoalcohol derivatives show in vitro anthelmintic activity against Trichuris muris and Heligmosomoides polygyrus

[EN]Background: Infections by gastrointestinal nematodes cause significant economic losses and disease in both humans and animals worldwide. The discovery of novel anthelmintic drugs is crucial for maintaining control of these parasitic infections. Methods: For this purpose, the aim of the present study was to evaluate the potential anthelmintic activity of three series of compounds against the gastrointestinal nematodes Trichuris muris and Heligmosomoides polygyrus in vitro. The compounds tested were derivatives of benzimidazole, lipidic aminoalcohols and diamines. A primary screening was performed to select those compounds with an ability to inhibit T. muris L1 motility by > 90% at a single concentration of 100 µM; then, their respective IC50 values were calculated. Those compounds with IC50 < 10 µM were also tested against the adult stage of T. muris and H. polygyrus at a single concentration of 10 µM. Results: Of the 41 initial compounds screened, only compounds AO14, BZ6 and BZ12 had IC50 values < 10 µM on T. muris L1 assay, showing IC50 values of 3.30, 8.89 and 4.17 µM, respectively. However, only two of them displayed activity against the adult stage of the parasites: BZ12 killed 81% of adults of T. muris (IC50 of 8.1 µM) and 53% of H. polygyrus while BZ6 killed 100% of H. polygyrus adults (IC50 of 5.3 µM) but only 17% of T. muris. Conclusions: BZ6 and BZ12 could be considered as a starting point for the synthesis of further structurally related compounds.SIFinancial support came from MINECO: RETOS (AGL2016-79813-C2-1R/2R) and Junta de Castilla y León co-financed by FEDER, UE [LE020P17]. EVG was funded by FPU17/00627; VCGA and MAB are recipients of Junta de Castilla y Leon (JCyL) (LE082-18, LE051-18, respectively) and MMV by the Spanish “Ramon y Cajal” Programme (Ministerio de Economía y competitividad; MMV, RYC-2015-18368