Dietary Inclusion of Halobacterium salinarum Modulates Growth Performances and Immune Responses in Farmed Gilthead Seabream (Sparus aurata L.)

The use of natural immunostimulants is considered the most promising alternative to promote fish health, productive performance and quality, increasing the aquaculture profitability, sustainability and social acceptance. The purpose of this study was to evaluate the effect of the integration of a potential probiotic strain, Halobacterium salinarum, belonging to the Archaea domain, in the formulated diets of farmed gilthead seabream (Sparus aurata L.) in terms of growth performances and immunity responses. The experiment was set up to test two different levels of inclusion of the bacteria in the diet: 0.05% (D1) and 0.1% (D2). The effects on fish growth performances; humoral (peroxidase, protease, antiprotease and IgM levels) and cellular immunity parameters (phagocytosis, respiratory burst and myeloperoxidase), along with bactericidal activity, were evaluated after 15 and 30 days of experimental feeding. The obtained results showed that the inclusion of H. salinarum at the highest concentration (D2 0.1%) improved growth performances, bactericidal activity against Vibrio anguillarum and some parameters related both to the humoral and cellular immune response, suggesting exploring other aspects of welfare in view of future supplementations of this probiotic strain in the diet of S. aurata

Estudio de coste-efectividad de mastectomía contralateral profiláctica

Analizar si la mastectomía contralateral profiláctica es más coste efectiva que el resto de cirugía

La fortaleza altomedieval del Cerro Calvario, en La Puebla de Castro : un "Hisn" en el extremo norte de la Marca Superior de Al-Ándalus

Bibliografía p. 156-173

Terminal carbohydrates abundance, immune related enzymes, bactericidal activity and physico-chemical parameters of the Senegalese sole (Solea senegalensis, Kaup) skin mucus

Recently, interest in mucosal surfaces, more specifically fish skin and its secreted mucus, has greatly increased among immunologists. The abundance of terminal carbohydrates, several enzymes (proteases, lysozyme, peroxidase, alkaline phosphatase, esterases and ceruloplasmin), bactericidal activity against fish pathogenic and non-pathogenic bacteria and several physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolarity, density and viscosity) in the skin mucus of Senegalese sole (Solea senegalensis, Kaup) have been evaluated. Present results evidence the abundance of N-acetylneuraminic acid, mannose, glucose and N-acetyl-galactosamine in skin mucus. The levels of lysozyme, proteases, esterases and alkaline phosphatase were very similar (from 20 to 30 Units mg 1 protein). However, 93 Units mg 1 protein were detected of ceruloplasmin and only 4 0 88 Units mg 1 protein of peroxidase. Skin mucus of S. senegalensis showed high bactericidal activity against the tested pathogen bacteria but weak activity against non-pathogenic bacteria. Finally, a clear relationship between mucus density and temperature was detected, while viscosity showed a direct shear- and temperature-dependent behaviour. These results could be useful for better understanding the role of the skin mucus as a key component of the innate immune system, as well as, for elucidating possible relationships between biological and physico-chemical parameters and disease susceptibility

Concepción Bonet Muñoz (1916-2014). Pioneer of professional geology and photogeology in Spain

Concepción Bonet Muñoz (1916-2014) fue la primera mujer que se incorporó a la geología profesional y, además contribuyó en la implementación de la fotogeología en España. Cursó Ciencias Naturales en Madrid, graduándose en 1943. Se dedicó a la enseñanza, y en 1950 obtuvo una beca del CSIC para especializarse como paleontóloga. Entre 1953 y 1954, realizó una estancia en la Universidad de Heerlen (Holanda) para estudiar las megasporas carboníferas. Defendió su Tesis Doctoral en 1955. Entre 1954 y 1956 dió clases en la Facultad de Ciencias Naturales. Se especializó en Fotogeología y entre 1956 y 1973 impartió clases de fotogeología. En 1957 ingresó en la Junta de Energía Nuclear, dónde creó el Departamento de Fotogeología y realizó varias estancias de especialización en el extranjero. En 1964 entró en la Dirección General de Carreteras del Ministerio de Obras Públicas, donde permaneció hasta que se jubiló en 1984. Trabajó en el desarrollo del Plan General de Carreteras y participó en los Estudios Geológicos Previos de las principales carreteras, donde influyó en la metodología de trabajo, incluyendo los fotoplanos geológicos como herramienta fundamental de trabajo, tanto por su aportación al conocimiento de la geomorfología como por su utilidad para buscar materias primas para la obra públicaConcepción Bonet Muñoz (1916-2014) was the first woman who joined professional geology, and also she contributed to the implementation of photogeology in Spain. She studied Natural Sciences at Madrid University, finally she was graduated in 1943. She devoted herself to teaching, and in 1950 she obtained a CSIC research grant to specialize as a palaeontologist. Between 1953 and 1954, she got a research grant to stay at the University of Heerlen (Holland) to study the carboniferous megasporas. She completed her PhD in 1955. Between 1954 and 1956 she taught at the Faculty of Natural Sciences. She specialized in Photogeology and between 1956 and 1973 taught photogeology classes. In 1957 she joined the Nuclear Energy Board, where she created the Department of Photogeology and made several specialization stays abroad. In 1964 she joined the Department of Roads of the Ministry of Public Works, where she retired in 1984. She joined the development team of the General Road Plan and participated in the Previous Geological Studies of the main roads, where she strongly influenced the work methodology, making geological photoplanes the fundamental work tool, both for her contribution to the knowledge of geomorphology and for its usefulness in looking for raw materials for public work

Activity of grindelanes against important maize pest Spodoptera frugiperda and their selectivity of action on non-target environmental bacteria

In the search for new plant-derived products with antifeedant and insecticidal potential against economically important insects, 11 grindelanes (two as natural acids and nine as methylated derivatives) purified from the foliar tissue of Grindelia chiloensis (Cornel.) Cabrera (Asteraceae) were evaluated against the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Larvae of this moth are considered to be the main pest of maize that causes severe losses on local agricultural production. Remarkable alterations in feeding behavior, larval weight, larval and pupal stage lengths, and adult fertility, as well as malformations and mortality in specimens during their life cycle were noticed in the feeding preference tests and no-choice diet assays. Antifeedant indexes (AI%) calculated in food preference bioassays reached up to 79% for the lipophilic grindelane 3b, the most potent antifeedant agent. Grindelane 7b showed a better insecticidal activity (median lethal dose, LD50 = 26.49 µg g−1) and 8b was potentially neurotoxic due to acetylcholinesterase inhibition. Ultrastructure of the middle portion of the digestive tract of larvae fed with small amounts of natural grindelane 9a supplemented to the diet revealed cell damage, and thickening of the peritrophic membrane and a striking number of mitochondria, as evident signs of defense against toxic substances ingested by the larvae. Besides, this study demonstrated that these effective insecticides did not have inhibitory effects on beneficial plant and soil bacteria which would allow ecological pest management.Fil: Mesurado, María de Los Angeles. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Orgánica; ArgentinaFil: Chalup, Adriana Elizabeth. Fundación Miguel Lillo. Dirección de Zoología. Instituto de Entomología; ArgentinaFil: Ortiz, Javier Esteban. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Zaragoza Puchol, José Daniel. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Bardon, Alicia del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cartagena, Elena. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentin

Characterization of a population of helicotylenchus dihystera (cobb, 1893) sher, 1961, parasitizing maize roots, in southern Córdoba, Argentina

Los nematodos en espiral del género Helicotylenchus, son parásitos de raíz de numerosas plantas cultivadas y no cultivadas. La identificación específica puede resultar compleja porque comparten diferentes caracteres diagnósticos similares y muestran una considerable variabilidad interespecífica. Su reconocimiento resulta esencial para poder establecer estrategias apropiadas de control o para prevenir su propagación a otras áreas. En este trabajo se evaluaron caracteres morfológicos y caracteres morfométricos de una población relacionada con el cultivo de maíz. Al mismo tiempo, análisis moleculares por PCR de una región del D2-D3 del gen 28s y la región 5.8s-ITS2 permitieron generar dos nuevas secuencias que fueron depositadas en Genbank. Además, por análisis filogenéticos utilizando inferencia Bayesiana, se complementó la identificación de la especie. Este trabajo constituye la primera caracterización morfológica, morfométrica y molecular de una población de Helicotylenchus dihystera para Argentina.Fil: Brücher, Elsa. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Vuletic, Ezequiel Esteban.Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Guerra, Fernando Andrés. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: De Rossi, Roberto Luis. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Plazas, María Cristina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Guerra, Gustavo Darío. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentin

Autophagy and ER-stress in BPAN: insights from the Dictyostelium model

Trabajo presentado en First BPAN disease symposium in France, celebrado en Lyon (Francia) del 13 al 14 de mayo de 2022.WIPIs are a conserved family of proteins with a characteristic 7-bladed b-helix structure. Mutations in human WIPI4 cause BPAN, a rare disease characterized by developmental delay, motor disorders and seizures. The pathological mechanisms leading to BPAN are still unclear and it has been proposed that autophagy, mitochondria homeostasis and endoplasmic reticulum (ER) stress are involved. We have generated a KO mutant in the WIPI4 homologue of Dictyostelium, Wdr45l. This mutant exhibits a complete block in autophagy that is associated with additional phenotypes: increased PI3P, growth arrest, and chronic activation of ER stress. PI3P accumulation is responsible for the growth arrest and chronic ER stress response and can therefore be considered as a potential therapeutic target. We have also analyzed the functionality of a number of pathological variants of WIPI4 with nonsense. Our results show that some of these mutations affect the subcellular localization of the protein, while others impair ATG2 binding

Effects of HCV Eradication on Bone mineral density in HIV/HCV Coinfected Patients

Little is known about the effects of eradication of HCV on bone mineral density (BMD) and biomarkers of bone remodeling in HIV/HCV coinfected patients. We prospectively assessed standardized BMD (sBMD) at the lumbar spine and femoral neck, World Health Organization (WHO) BMD categories at both sites, and plasma concentrations of soluble receptor activator of nuclear factor-kappaβ ligand (sRANKL), and osteoprotegerin (OPG) at baseline (the date of initiation of anti-HCV therapy) and at 96 weeks. A total of 238 patients were included, median age 49.5 years, 76.5% males, 48.3% with cirrhosis, 98.3% on antiretroviral therapy, median CD4+ cell count 527 cells/mm 3, 86.6% with HIV-1 RNA < 50 copies/mL. The prevalence of osteoporosis at baseline at the lumbar spine (LS) and femoral neck (FN) was 17.6% and 7.2%, respectively. Anti-HCV therapy comprised pegylated interferon and ribavirin (PegIFN-RBV) plus one direct-acting antiviral in 53.4%, PegIFN-RBV in 34.5%, and sofosbuvir/RBV in 12.2%. A total of 145 (60.9%) patients achieved sustained viral response (SVR). No significant effect of SVR was observed on sBMD for the interaction between time and SVR either in the LS (P=0.801) or the FN (P=0.911). Likewise, no significant effect of SVR was observed in plasma levels of sRANKL (P=0.205), OPG (P=0.249), and sRANKL/OPG ratio (P=0.123) for the interaction between time and SVR. No significant correlation was found between fibrosis by transient elastography, and LS and FN sBMD, at baseline, and week 96. SVR was not associated with significant changes in BMD nor biomarkers of bone remodeling in HIV/HCV-coinfected persons.This study was supported by Instituto de Salud Carlos III (ISCII), grant numbers PI11/01556, PI14/01094, PI14/01581, and PI14CIII/00011, and by Ministerio de Sanidad, Servicios Sociales e Igualdad, grant number EC11-241. The study was also funded by the RD16/0025/0017, RD16/0025/0018 and RD16CIII/0002/0002 projects as part of the Plan Nacional R + D + I and cofunded by ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER).S

Composición farmacéutica y medicamento de liberación controlada de morfina basado en un complejo de morfina con un polímero acrílico

Composición farmacéutica y medicamento de liberación controlada de morfina basado en un complejo de morfina con un polímero acrílico. La composición farmacéutica comprende un complejo de liberación controlada de morfina que contiene morfina complejada con un polímero acrílico, una sal farmacéuticamente aceptable de morfina libre, y uno o más excipientes inertes respecto a la liberación controlada. Dicha composición es útil para elaborar un comprimido oral de liberación controlada de morfina para el tratamiento del dolor crónico severo sin necesidad de tener que administrar simultáneamente morfina vía parenteral.Españ