Survival and Movement of Adult Rainbow Trout During Winter and Spring in the Henrys Fork of the Snake River

Discharge downstream from Island Park Dam on the Henrys Fork of the Snake River in Idaho is reduced each winter to facilitate storage of irrigation water. The effect this has on survival and movement of adult rainbow trout (Oncorhynchus mykiss) in this area is unknown. Additionally, fish movement during the spring has not been evaluated but may affect population estimates conducted in the tailwater monitoring area downstream from Island Park Dam prior to opening of fishing season. Therefore, we used radio telemetry to evaluate winter survival and movement of 61 adult rainbow trout in the Henrys Fork downstream from Island Park Dam under low and extremely low early winter flow conditions. Spring movement was also evaluated to asses whether the population estimates conducted in the monitoring area each spring represent fish from downstream adjacent reaches of the river, and how emigration between mark and recapture periods may affect the population estimate. Survival of radio-tagged trout was nearly 100 percent during early winter under both low and extremely low flow conditions and winter movement did not differ between the two years. Few radio-tagged rainbow trout from downriver were present in the monitoring reach during the time when the population estimate is normally conducted, indicating that large fluctuations in fish numbers in downstream reaches would likely be undetected based on population estimates conducted in the monitoring area. To remedy this, establishing a separate, regular population monitoring area in downstream reaches is recommended. We determined emigration from the monitoring reach between mark and recapture to have a minimal effect on the population estimate. However, we noted that all radio-tagged trout moving out of the monitoring reach during May moved into a short section of river between the monitoring reach and Island Park Dam, presumably to spawn. Therefore, emigration could be largely eliminated by extending the monitoring reach upstream to the dam

Foliar Water Uptake: Processes, Pathways, and Integration into Plant Water Budgets

Nearly all plant families, represented across most major biomes, absorb water directly through their leaves. This phenomenon is commonly referred to as foliar water uptake. Recent studies have suggested that foliar water uptake provides a significant water subsidy that can influence both plant water and carbon balance across multiple spatial and temporal scales. Despite this, our mechanistic understanding of when, where, how, and to what end water is absorbed through leaf surfaces remains limited. We first review the evidence for the biophysical conditions necessary for foliar water uptake to occur, focusing on the plant and atmospheric water potentials necessary to create a gradient for water flow. We then consider the different pathways for uptake, as well as the potential fates of the water once inside the leaf. Given that one fate of water from foliar uptake is to increase leaf water potentials and contribute to the demands of transpiration, we also provide a quantitative synthesis of observed rates of change in leaf water potential and total fluxes of water into the leaf. Finally, we identify critical research themes that should be addressed to effectively incorporate foliar water uptake into traditional frameworks of plant water movement

Quantitative Analysis of Protein Dynamics during Asymmetric Cell Division

SummaryIn dividing Drosophila sensory organ precursor (SOP) cells, the fate determinant Numb and its associated adaptor protein Pon localize asymmetrically and segregate into the anterior daughter cell, where Numb influences cell fate by repressing Notch signaling. Asymmetric localization of both proteins requires the protein kinase aPKC and its substrate Lethal (2) giant larvae (Lgl) [1–3]. Because both Numb and Pon localization require actin and myosin [4–6], lateral transport along the cell cortex has been proposed as a possible mechanism for their asymmetric distribution [5]. Here, we use quantitative live analysis of GFP-Pon and Numb-GFP fluorescence and fluorescence recovery after photobleaching (FRAP) to characterize the dynamics of Numb and Pon localization during SOP division. We demonstrate that Numb and Pon rapidly exchange between a cytoplasmic pool and the cell cortex and that preferential recruitment from the cytoplasm is responsible for their asymmetric distribution during mitosis. Expression of a constitutively active form of aPKC impairs membrane recruitment of GFP-Pon. This defect can be rescued by coexpression of nonphosphorylatable Lgl, indicating that Lgl is the main target of aPKC. We propose that a high-affinity binding site is asymmetrically distributed by aPKC and Lgl and is responsible for asymmetric localization of cell-fate determinants during mitosis

High-temperature superconductivity in doped antiferromagnets

In the context of an effective model for doped antiferromagnets, whereby the charge carriers are treated as hard-core bosons, we demonstrate that the ground state energy close to half-filling is an even periodic function of the external magnetic flux threading the square lattice in an Aharonov-Bohm geometry. The period is equal to the flux quantum $\Phi_{0}=2\pi\hbar c/q$ entering the Peierls phase factor of the hopping matrix elements. Thus flux quantization and a concomitant finite value of superfluid weight D_{s} occur along with metallic antiferromagnetism. We argue that the charge q in the associated flux quantum might be set equal to 2e. The superconducting transition temperature T_{c} is related to D_{s} linearly, in accordance to the generic Kosterlitz-Thouless type of transition in a two-dimensional system, signalling the coherence of the phase fluctuations of the condensate. The calculated dependence of T_{c} on hole concentration is qualitatively similar to that observed in the high-temperature superconducting cuprates.Comment: 5 pages, REVTEX file (2 Postscript figures). Proc. of 1st Euroconference on "Anomalous Complex Superconductors". To appear in Physica C (1999

Neural population partitioning and a concurrent brain-machine interface for sequential motor function

Although brain-machine interfaces (BMIs) have focused largely on performing single-targeted movements, many natural tasks involve planning a complete sequence of such movements before execution. For these tasks, a BMI that can concurrently decode the full planned sequence before its execution may also consider the higher-level goal of the task to reformulate and perform it more effectively. Using population-wide modeling, we discovered two distinct subpopulations of neurons in the rhesus monkey premotor cortex that allow two planned targets of a sequential movement to be simultaneously held in working memory without degradation. Such marked stability occurred because each subpopulation encoded either only currently held or only newly added target information irrespective of the exact sequence. On the basis of these findings, we developed a BMI that concurrently decodes a full motor sequence in advance of movement and can then accurately execute it as desired.National Institutes of Health (U.S.) (DP1 OD003646

A Real-Time Brain-Machine Interface Combining Motor Target and Trajectory Intent Using an Optimal Feedback Control Design

Real-time brain-machine interfaces (BMI) have focused on either estimating the continuous movement trajectory or target intent. However, natural movement often incorporates both. Additionally, BMIs can be modeled as a feedback control system in which the subject modulates the neural activity to move the prosthetic device towards a desired target while receiving real-time sensory feedback of the state of the movement. We develop a novel real-time BMI using an optimal feedback control design that jointly estimates the movement target and trajectory of monkeys in two stages. First, the target is decoded from neural spiking activity before movement initiation. Second, the trajectory is decoded by combining the decoded target with the peri-movement spiking activity using an optimal feedback control design. This design exploits a recursive Bayesian decoder that uses an optimal feedback control model of the sensorimotor system to take into account the intended target location and the sensory feedback in its trajectory estimation from spiking activity. The real-time BMI processes the spiking activity directly using point process modeling. We implement the BMI in experiments consisting of an instructed-delay center-out task in which monkeys are presented with a target location on the screen during a delay period and then have to move a cursor to it without touching the incorrect targets. We show that the two-stage BMI performs more accurately than either stage alone. Correct target prediction can compensate for inaccurate trajectory estimation and vice versa. The optimal feedback control design also results in trajectories that are smoother and have lower estimation error. The two-stage decoder also performs better than linear regression approaches in offline cross-validation analyses. Our results demonstrate the advantage of a BMI design that jointly estimates the target and trajectory of movement and more closely mimics the sensorimotor control system.National Institutes of Health (U.S.) (NIH grant No.DP1-0D003646-01)National Institutes of Health (U.S.) (NIH grant R01-EB006385

Flux quantization and superfluid weight in doped antiferromagnets

Doped antiferromagnets, described by a t-t'-J model and a suitable 1/N expansion, exhibit a metallic phase-modulated antiferromagnetic ground state close to half-filling. Here we demonstrate that the energy of latter state is an even periodic function of the external magnetic flux threading the square lattice in an Aharonov-Bohm geometry. The period is equal to the flux quantum $\Phi_{0}=2\pi\hbar c/q$ entering the Peierls phase factor of the hopping matrix elements. Thus flux quantization and a concomitant finite value of superfluid weight D_s occur along with metallic antiferromagnetism. We argue that in the context of the present effective model, whereby carriers are treated as hard-core bosons, the charge q in the associated flux quantum might be set equal to 2e. Finally, the superconducting transition temperature T_c is related to D_s linearly, in accordance to the generic Kosterlitz-Thouless type of transition in a two-dimensional system, signaling the coherence of the phase fluctuations of the condensate. The calculated dependence of T_c on hole concentration is qualitatively similar to that observed in the high-temperature superconducting cuprates.Comment: 5 pages, 2 figures, to be published in J. Phys. Condens. Matte

Neural population partitioning and a concurrent brain-machine interface for sequential motor function

While brain-machine interfaces (BMIs) have largely focused on performing single-targeted movements, many natural tasks involve planning a complete sequence of such movements before execution. For these tasks, a BMI that can concurrently decode the full planned sequence prior to its execution may also consider the higher-level goal of the task to reformulate and perform it more effectively. Here, we show that concurrent BMI decoding is possible. Using population-wide modeling, we discover two distinct subpopulations of neurons in the rhesus monkey premotor cortex that allow two planned targets of a sequential movement to be simultaneously held in working memory without degradation. Such surprising stability occurred because each subpopulation encoded either only currently held or only newly added target information irrespective of the exact sequence. Based on these findings, we develop a BMI that concurrently decodes a full motor sequence in advance of movement and then can accurately execute it as desired

The trans-membrane protein p25 forms highly specialized domains that regulate membrane composition and dynamics

Trans-membrane proteins of the p24 family are abundant, oligomeric proteins predominantly found in cis-Golgi membranes. They are not easily studied in vivo and their functions are controversial. We found that p25 can be targeted to the plasma membrane after inactivation of its canonical KKXX motif (KK to SS, p25SS), and that p25SS causes the co-transport of other p24 proteins beyond the Golgi complex, indicating that wild-type p25 plays a crucial role in retaining p24 proteins in cis-Golgi membranes. We then made use of these observations to study the intrinsic properties of these proteins, when present in a different membrane context. At the cell surface, the p25SS mutant segregates away from both the transferrin receptor and markers of lipid rafts, which are enriched in cholesterol and glycosphingolipids. This suggests that p25SS localizes to, or contributes to form, specialized membrane domains, presumably corresponding to oligomers of p25SS and other p24 proteins. Once at the cell surface, p25SS is endocytosed, together with other p24 proteins, and eventually accumulates in late endosomes, where it remains confined to well-defined membrane regions visible by electron microscopy. We find that this p25SS accumulation causes a concomitant accumulation of cholesterol in late endosomes, and an inhibition of their motility - two processes that are functionally linked. Yet, the p25SS-rich regions themselves seem to-exclude not only Lamp1 but also accumulated cholesterol. One may envision that p25SS accumulation, by excluding cholesterol from oligomers, eventually overloads neighboring late endosomal membranes with cholesterol beyond their capacity (see Discussion). In any case, our data show that p25 and presumably other p24 proteins are endowed with the intrinsic capacity to form highly specialized domains that control membrane composition and dynamics. We propose that p25 and other p24 proteins control the fidelity of membrane transport by maintaining cholesterol-poor membranes in the Golgi complex