GLOVEBOX DISMANTLEMENT AND EQUIPMENT PROTECTION IN CONTAMINATED ENVIRONMENTS

ABSTRACT It has been revealed from the experiences of Decontamination and Decommissioning (D&D) activities that even a small improvement in performance can result in significant risk reduction and cost savings. For example, Race Scan Ear Mic System, which was originally developed for communications between racecar drivers and crews in loud environments, has been successfully applied to D&D work and proved to enhance worker safety and communications. Glovebox dismantlement is an important and costly process in D&D activities of nuclear facilities. Adequate decontamination and size reduction of the gloveboxes are especially important in this activity because they have the potential to reduce risks and costs significantly. This paper presents some simple approaches to support D&D tasks and discusses their potential advantages. Examples discussed include: • Repeated shear wiping of large pipes and ducts • Application of thin layers on radiological counters for uninterrupted use • Partial use of robotics for glovebox dismantling The paper also discusses schematics for protecting equipment interiors and/or glovebox inner surfaces from contamination, which may result in significant savings and waste minimization upon future dismantlement. Examples discussed include: • Smart coating for contamination prevention • Protecting equipment by geometrically simple cove

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Glovebox Dismantlement and Equipment Protection in Contaminated Environments

It has been revealed from the experiences of Decontamination and Decommissioning (D&D) activities that even a small improvement in performance can result in significant risk reduction and cost savings. For example, Race Scan Ear Mic System, which was originally developed for communications between racecar drivers and crews in loud environments, has been successfully applied to D&D work and proved to enhance worker safety and communications. Glovebox dismantlement is an important and costly process in D&D activities of nuclear facilities. Adequate decontamination and size reduction of the gloveboxes are especially important in this activity because they have the potential to reduce risks and costs significantly. This paper presents some simple approaches to support D&D tasks and discusses their potential advantages. Examples discussed include: Repeated shear wiping of large pipes and ducts; Application of thin layers on radiological counters for uninterrupted use; and Partial use of robotics for glovebox dismantling. The paper also discusses schematics for protecting equipment interiors and/or glovebox inner surfaces from contamination, which may result in significant savings and waste minimization upon future dismantlement. Examples discussed include: Smart coating for contamination prevention; and Protecting equipment by geometrically simple cover

Conducting a large, multi-site survey about patients’ views on broad consent: challenges and solutions

Abstract Background As biobanks play an increasing role in the genomic research that will lead to precision medicine, input from diverse and large populations of patients in a variety of health care settings will be important in order to successfully carry out such studies. One important topic is participants’ views towards consent and data sharing, especially since the 2011 Advanced Notice of Proposed Rulemaking (ANPRM), and subsequently the 2015 Notice of Proposed Rulemaking (NPRM) were issued by the Department of Health and Human Services (HHS) and Office of Science and Technology Policy (OSTP). These notices required that participants consent to research uses of their de-identified tissue samples and most clinical data, and allowing such consent be obtained in a one-time, open-ended or “broad” fashion. Conducting a survey across multiple sites provides clear advantages to either a single site survey or using a large online database, and is a potentially powerful way of understanding the views of diverse populations on this topic. Methods A workgroup of the Electronic Medical Records and Genomics (eMERGE) Network, a national consortium of 9 sites (13 separate institutions, 11 clinical centers) supported by the National Human Genome Research Institute (NHGRI) that combines DNA biorepositories with electronic medical record (EMR) systems for large-scale genetic research, conducted a survey to understand patients’ views on consent, sample and data sharing for future research, biobank governance, data protection, and return of research results. Results Working across 9 sites to design and conduct a national survey presented challenges in organization, meeting human subjects guidelines at each institution, and survey development and implementation. The challenges were met through a committee structure to address each aspect of the project with representatives from all sites. Each committee’s output was integrated into the overall survey plan. A number of site-specific issues were successfully managed allowing the survey to be developed and implemented uniformly across 11 clinical centers. Conclusions Conducting a survey across a number of institutions with different cultures and practices is a methodological and logistical challenge. With a clear infrastructure, collaborative attitudes, excellent lines of communication, and the right expertise, this can be accomplished successfully

Sequencing of neuroblastoma identifies chromothripsis and defects in neuritogenesis genes

Neuroblastomais a childhood tumour of the peripheral sympathetic nervous system. The pathogenesis has for a long time been quite enigmatic, as only very few gene defects were identified in this often lethal tumour(1). Frequently detected gene alterations are limited to MYCN amplification (20%) and ALK activations (7%)(2-5). Here we present a whole-genome sequence analysis of 87 neuroblastoma of all stages. Few recurrent amino-acid-changing mutations were found. In contrast, analysis of structural defects identified a local shredding of chromosomes, known as chromothripsis, in 18% of high-stage neuroblastoma(6). These tumours are associated with a poor outcome. Structural alterations recurrently affected ODZ3, PTPRD and CSMD1, which are involved in neuronal growth cone stabilization(7-9). In addition, ATRX, TIAM1 and a series of regulators of the Rac/Rho pathway were mutated, further implicating defects in neuritogenesis in neuroblastoma. Most tumours with defects in these genes were aggressive high-stage neuroblastomas, but did not carry MYCN amplifications. The genomic landscape of neuroblastoma therefore reveals two novel molecular defects, chromothripsis and neuritogenesis gene alterations, which frequently occur in high-risk tumours