Such News Of The Land: U.S. Women Nature Writers

This pathbreaking collection, which contains 19 essays from scholars in a variety of fields, illuminates the work of two centuries of American women nature writers. Some discuss traditional nature writers such as Susan Fenimore Cooper, Mary Austin, Gene Stratton Porter, and Annie Dillard. Others examine the work of Zora Neale Hurston, Gloria Anzaldua, and Leslie Marmon Silko, writers not often associated with this genre. Essays on germinal texts such as Marjory Stoneman Douglas\u27s The Everglades: River of Grass stand alongside examinations of market bulletins and women\u27s gardens, showing how the rich diversity of women\u27s nature writing has shaped and expanded the genre, and enlarged the audience for whom nature mattered. This second, digital edition contains an updated introduction and author biographies.https://dune.une.edu/history_facbooks/1000/thumbnail.jp

Time Spent on Higher-Order Tasks in Two Teacher-Apprentice Options

This study investigates the process of assisting a teacher prior to student teaching. Teacher candidates in two certification programs kept a log documenting the tasks they did during their on-site experiences and the time spent on eight types of tasks. A one-way analysis for repeated measures showed that, in both programs, candidates spent a majority of their time on clerical tasks, despite the value they saw in interpersonal contact. A t test for independent measures revealed no significant difference between constant and varied placements in time spent on teaching a whole class and on other highly valued tasks

Phosphorylation of androgen receptors at serine 515 is a potential prognostic marker for triple negative breast cancer

1.7 million cases of breast cancer are diagnosed every year with 522,000 deaths. Molecular classifications of breast cancer have resulted in improved treatments. However, treatments for triple negative breast cancer (TNBC) are lacking. Analysis of molecular targets for TNBC is a priority. One potential candidate is androgen receptor (AR) phosphorylation. This study assessed the role of AR phosphorylation at ser81/ser515 and their two upstream effectors, cyclin-dependent kinase 1 (pCDK1) and extracellular-regulated kinase 1/2 (pERK1/2) in 332 ductal breast cancer patients by immunohistochemistry. pERK1/2 combined with AR-515 associated with improved cancer-specific survival (CSS, p = 0.038), decreased size (p = 0.001), invasive grade (p < 0.001), necrosis (p = 0.003), b-lymphocytes (p = 0.020), molecular subtype (p < 0.001) and estrogen receptor (ER)/progesterone receptor (PR)-status (p < 0.001). The cohort was therefore stratified into ER+ve and ER-ve patients. In ER+ve tumours, pERK1/2 combined with AR-515 associated with improved CSS (p = 0.038), smaller size (p = 0.004), invasive grade (p = 0.001), decreased b-lymphocytes (p = 0.013) and increased plasma cells (p = 0.048). In contrast, in TNBC patients, phosphorylation of AR-515 associated with poorer CSS (p = 0.007). pERK1/2 combined with AR-515 associated with decreased inflammation (p = 0.003), increased tumour stroma (p = 0.003) and tumour budding (p = 0.011), with trends towards decrease CSS (p = 0.065) and macrophage levels (p = 0.093). In Conclusions, AR-515 may be an important regulator of inflammation in breast cancer potential via ERK1/2 phosphorylation. AR-515 is a potential prognostic marker and therapeutic target for TNBC

Quality of Runoff from Plots Treated with Municipal Sludge and Horse Bedding

Land application of horse stall bedding and municipal sludge can increase runoff concentrations of nutrients, organic matter, and bacteria as well as steroidal hormones such as estrogen. Concentrations of materials in runoff from sites treated with animal manure can be reduced by aluminum sulfate, or alum [Al2(SO4)3•14H2O] treatment. The objectives of this study were to assess plots treated with horse stall bedding or municipal sludge for: (a) runoff quality [concentrations of nitrate nitrogen (NO3-N), ammonia nitrogen (NH3-N), orthophosphate-phosphorus (PO4-P), fecal coliform (FC), chemical oxygen demand (COD) and 17- β estradiol (17 β-E, a form of estrogen)]; (b) changes in runoff quality caused by alum treatment; and (c) time variations in concentrations of the analysis parameters. Horse bedding and municipal sludge were applied to twelve 2.4 × 6.1 m fescue plots (six each for the bedding and sludge). Three of the bedding-treated and three of the sludge-treated plots were also treated with alum. Simulated rainfall (64 mm/h) was applied to the 12 treated plots and to three control (no treatment) plots. The data were analyzed as originating from separate completely randomized, one-way designs with three replications of each treatment. The first design had treatment levels of bedding, bedding and sludge, and control, while the second design had treatment levels of sludge, sludge and alum, and control. The control data were common to both designs. The first 0.5 h runoff was sampled and analyzed for the parameters described above. Analysis parameter concentrations for the waste treated plots were generally lower than those previously reported for runoff after organic treatments. In some cases, concentrations were no different from the controls. Mass losses of all parameters were low and agronomically insignificant. Alum addition decreased runoff PO4-P concentrations and increased NO3-N concentrations but had no effect on concentrations of other parameters. A significant effect of alum addition on 17 β-E and COD concentrations was anticipated on the basis of previous studies; its absence might have been due to inadequate mixing or interval between addition and simulated rainfall. Relationships between concentration and collection time followed two patterns: (a) highest concentrations occurring during the first sample (two minutes following runoff initiation; NO3-N, COD, FC and 17 β-E) and (b) delay in peak concentration until four minutes following runoff initiation (NH3-N and PO4-P). The detection of different general relationships between concentration and time suggests that different mechanisms are dominant in transport of the parameters analyzed

Characterizing the metabolism of Dehalococcoides with a constraint-based model

Dehalococcoides strains respire a wide variety of chloro-organic compounds and are important for the bioremediation of toxic, persistent, carcinogenic, and ubiquitous ground water pollutants. In order to better understand metabolism and optimize their application, we have developed a pan-genome-scale metabolic network and constraint-based metabolic model of Dehalococcoides. The pan-genome was constructed from publicly available complete genome sequences of Dehalococcoides sp. strain CBDB1, strain 195, strain BAV1, and strain VS. We found that Dehalococcoides pan-genome consisted of 1118 core genes (shared by all), 457 dispensable genes (shared by some), and 486 unique genes (found in only one genome). The model included 549 metabolic genes that encoded 356 proteins catalyzing 497 gene-associated model reactions. Of these 497 reactions, 477 were associated with core metabolic genes, 18 with dispensable genes, and 2 with unique genes. This study, in addition to analyzing the metabolism of an environmentally important phylogenetic group on a pan-genome scale, provides valuable insights into Dehalococcoides metabolic limitations, low growth yields, and energy conservation. The model also provides a framework to anchor and compare disparate experimental data, as well as to give insights on the physiological impact of "incomplete" pathways, such as the TCA-cycle, CO 2 fixation, and cobalamin biosynthesis pathways. The model, referred to as iAI549, highlights the specialized and highly conserved nature of Dehalococcoides metabolism, and suggests that evolution of Dehalococcoides species is driven by the electron acceptor availability

Treating Opioid Use Disorders in Drug Court: Participants’ Views on Using Medication-Assisted Treatments (MATs) to Support Recovery

Drug courts began in 1989 in Miami-Dade County, FL. Due to their success in treating substance use disorders and reducing criminal recidivism, they have expanded globally and are currently operating in countries such as Australia, Canada, and Scotland, to name a few. Drug courts can be a key intervention in addressing the opioid epidemic. This is the first known qualitative study to ask drug court participants (n = 38) who have opioid use disorders questions related to their lived experiences in drug court, as well as direct questions related to the use of medication-assisted treatments (MATs) in drug court. Overall, drug court participants felt that MATs were helpful for treating their opioid use disorders; however, some participants reported using other drugs while on MATs and they viewed their recovery through a harm reduction lens. Additionally, participants emphasized the importance of using MATs in combination with counseling that used cognitive and behavioral therapies. Implications for drug court practice and future research are discussed

Expression of RUNX1 correlates with poor patient prognosis in triple negative breast cancer

The RUNX1 transcription factor is widely recognised for its tumour suppressor effects in leukaemia. Recently a putative link to breast cancer has started to emerge, however the function of RUNX1 in breast cancer is still unknown. To investigate if RUNX1 expression was important to clinical outcome in primary breast tumours a tissue microarray (TMA) containing biopsies from 483 patients with primary operable invasive ductal breast cancer was stained by immunohistochemistry. RUNX1 was associated with progesterone receptor (PR)-positive tumours (P<0.05), more tumour CD4+(P<0.05) and CD8+(P<0.01) T-lymphocytic infiltrate, increased tumour CD138+plasma cell (P<0.01) and more CD68+macrophage infiltrate (P<0.001). RUNX1 expression did not influence outcome of oestrogen receptor (ER)-positive or HER2-positive disease, however on univariate analysis a high RUNX1 protein was significantly associated with poorer cancer-specific survival in patients with ER-negative (P<0.05) and with triple negative (TN) invasive breast cancer (P<0.05). Furthermore, multivariate Cox regression analysis of cancer-specific survival showed a trend towards significance in ER-negative patients (P<0.1) and was significant in triple negative patients (P<0.05). Of relevance, triple negative breast cancer currently lacks good biomarkers and patients with this subtype do not benefit from the option of targeted therapy unlike patients with ER-positive or HER2-positive disease. Using multivariate analysis RUNX1 was identified as an independent prognostic marker in the triple negative subgroup. Overall, our study identifies RUNX1 as a new prognostic indicator correlating with poor prognosis specifically in the triple negative subtype of human breast cancer

Effects of Chemical Amendments to Swine Manure on Runoff Quality

Land-applied swine manure can be an environmental concern when runoff losses of manure constituents occur. The use of chemical amendments to mitigate these losses has been investigated for poultry litter, but materials such as swine manure have received less attention in this context, particularly at the plot scale or larger. The objective of this experiment was to evaluate the impacts of aluminum sulfate (alum; Al2(SO4)3), aluminum chloride (AlCl3), and ferric chloride (FeCl3) addition on runoff of selected constituents of land-applied swine manure. Manure was collected from feeder pigs fed a standard diet. Alum and FeCl3 were added at a stoichiometric ratio of 1.1:1 [Al:total P (TP)], and AlCl3 was added at a ratio of 1.3:1 [Al:TP]. The amended manure was incubated for six days prior to land application to fescue (Festuca arundinacea Schreber) plots. Simulated rainfall (100 mm h-1 for 0.5 h of runoff) was applied to the plots on the day of application and followed by two additional simulated rainfall events at 7 d intervals. Runoff samples were collected and analyzed for dissolved reactive phosphorus (DP), TP, ammonia nitrogen (NH3-N), nitrate nitrogen (NO3-N), total Kjeldahl nitrogen (TKN), total suspended solids (TSS), fecal coliform (FC), and 17 β-estradiol. Runoff concentrations of all constituents except NO3-N were highest for the first simulated rainfall event, approaching background levels thereafter. Relative to untreated manure, all chemical amendments were effective in reducing first-event DP, TP, and TKN concentrations. Both AlCl3 and FeCl3 reduced first-event NH3-N concentrations, and FeCl3 addition led to FC concentrations indistinguishable from the control (no manure) plots. The results indicate that these amendments have potential for promoting both environmental and agronomic benefits, implying that studies involving practicality and long-term considerations should be undertaken