THE INFLUENCE OF THE TREATMENT OF IMPULSE PRESSURE ON LIPID PEROXIDATION AND THE CONTENT OF ANTIOXIDANTS IN WHEAT GRAINS DURING AGING

Background. The aging of seeds results in physiological and biochemical changes such as decrease of the activity of antioxidants (AO), and structural and functional degradation of proteins due to lipid peroxidation (LP), as well as degradation of membranes and DNA. Purpose. Identification of the traits of the antioxidant system of wheat grains with powdery and vitreous endosperm during aging and under the influence of a stressor. Materials and methods. Soft wheat grains were treated with impulse pressure (IP) and stored for 3 years. Germination was determined. The content AO was determined by the o-phenanthroline method, and LP products were assessed by the reaction of malonic dialdehyde with thiobarbituric acid. Results. Germination of control seeds with powdery endosperm after storage for 3 years was the lowest (20%). In control part of seeds with vitreous endosperm, germination was 27%. ID increased the amount of seeds with vitreous endosperm and germination. The content of AO in control grains with powdery endosperm was higher than in vitreous grains, and the content of LP products was lower. After the treatment of IP the content of AO increased in vitreous grains; it did not change as compared to the control in powdery grains. The content of LP products in the grains treated with ID 11 MPa was lower than in the control variant. An increase in POL at ID 29 MPa indicates the accumulation of damage; Conclusion. During the storage of wheat grains, germination decreased mainly due to the appearance of dead grains. The greatest decrease in germination was observed in grains with powdery endosperm. IP promoted vitrification of biopolymers which led to an increase in germination as compared to the control after 3 years of storage. A higher content of AO in grains with vitreous endosperm after IP treatment and storage contributed to the preservation of grain viability during storage

Diabetes mellitus type 1 in childhood

Public organization “Russian Association of Endocrinologists”. Clinical guidlines

Interval Hypoxic Training in Rehabilitation Program for Adolescents with Overweight / Obesity and Comorbid Arterial Hypertension: Open-Label Randomized Study

Background. Searching for new strategies for the rehabilitation of adolescents with obesity and comorbid arterial hypertension (AHT) before significant pathological changes development in the cardiovascular system remains the urgent challenge. Objective. The aim of the study was to examine the effect of interval hypoxic training (IHT) on blood pressure (BP) levels in adolescents with overweight / obesity and comorbid AHT. Methods. Adolescents aged 14-17 years with body mass index SDS ≥ 1 and grade I AHT (mean level of systolic and/or diastolic BP ≥ 95th percentile for population of corresponding age, sex and height) have been randomized to the group «aerobic training» (treadmill walking) and “aerobic training + IHT” (usage of hypoxicator in intermittent operation cycles). All patients were on subcaloric diet (10% reduction in caloric intake for given age). Primary outcome measure was the difference between groups on systolic/diastolic BP levels according to 24-hour BP monitoring after completion of training program (10 classes each). The effects of IHT on body composition (bioelectrical impedance analysis), heart rate variability and psychoemotional state (Spielberger scale, assessment of health, activity and mood) were further estimated. Results. 43 patients were assigned to the index group, and 42 — to the control group. 67 patients have completed the research program. 5 patients (12%) from the IHT group and 13 patients (31%; p = 0.029) from the control group prematurely discontinued participation in the trial due to poor exercise tolerance. The groups were comparable in baseline systolic and diastolic BP. Decrease in BP occurred in both groups after 10 workouts. There were no differences in decrease value: mean difference for SBP was 2.4 mm Hg (95% CI -6.6 ... 1.8), for DBP — 0.2 mm Hg (-3.6 ... 4.0). However, the incidence of reaching the targeted SBP (< 95th percentile for the corresponding age and sex) after completion of the treatment was recorded in 66% patients in the experimental group and in 42% patients in the control group (p = 0,047). Positive dynamics in several indicators of heart rate variability and psychoemotional state were mentioned in the IHT group. Conclusion. The IHT implementation in the complex of rehabilitation program for adolescents with overweight / obesity and AHT has no additional positive effect on BP levels. However, the incidence of reaching the targeted SBP (< 95th percentile for the corresponding age and sex) after completion of the treatment was recorded in 66% patients in the experimental group and in 42% patients in the control group (p = 0,054)