Sinai and Norfa chicken diversity revealed by microsatellite markers

The present study aimed to outline the population differentiation of Sinai and Norfa chicken, native to Egypt, with microsatellite markers. Twenty microsatellite loci recommended by the Food and Agriculture Organization (FAO) were used. Fifty eight birds were sampled (29 for each strain: 12 males + 17 females). Data were collected and genetic diversity indicators were assessed utilizing the approaches implemented in FSTAT, Cervus 3.0.7 and GenAlEx 6.5 software programmes. A total number of 182 alleles were detected with an average value of 9.1 allele per locus. The expected heterozygosity was 6.625 and 6.343 in Norfa and Sinai chickens, respectively. Norfa chickens produced 15 private alleles, while there were 9 unique alleles detected in Sinai chickens (13.18% private alleles as a percentage of the total observed number of alleles). Fixation indices’ (FST, FIS, and FIT) values were 0.060, 0.410 and 0.438, respectively, across all 20 loci investigated. Results indicated that the studied populations were genetically differentiated. Consequently, they have high breeding potential. Efforts should be made to incorporate the other local chicken strains as unique genetic resources into conservation programmes. This should begin with proper management of these flocks to ensure the maintenance of their genetic diversity over time by avoiding inbreeding. Such information is likely to have a profound effect on the success of genetic improvement and completes information from phenotypes and biometric measurements of the domestic chickens in Egypt.Keywords: Egyptian, genetic diversity, local chicken, microsatellit

A TRIAL TO DETECT THE EFFECT OF STRAIN AND DIFFERENT SOURCES OF DRINKING WATER ON SOME PRODUCTIVE TRAITS IN BROILER CHICKS

The main goal of this experiment was to evaluate the effect of broiler strain and source of drinking water on some performance traits (body weight and carcass traits). Sample of water were taken from two different location sources, Giza governorate (as Nile water) and Qalubia governorate (as well water or ground water). Three different broiler strains were used in this study (Arbor acres, Hubbard and Cobb). Lead concentration (as a heavy metal element) and bacteriological analysis (total bacteria and fecal coliforms) in both water and carcass were carried out. The main results obtained can be summarized as follow: Arbor acres strain recorded the heaviest body at most ages compared to the other strains, the difference was highly significant at 5 and 6 weeks of ages. Total bacteria were significantly high in ground water compared to Nile water. Conversely, Coliforms bacteria count was significantly high in Nile water compared to ground water source. Regardless strain type, high positive correlation between lead concentration in drinking water and lead concentration in liver, kidney and carcass weight was observe

Sunflower oil-based hyperbranched alkyd/spherical ZnO nanocomposite modeling for mechanical and anticorrosive applications

Approaches for designing advanced nanomaterials with hyperbranched architectures and lack of volatile organic content (VOC) have attracted considerable attention. In this study, eco-friendly hyperbranched alkyd resins for mechanical and anticorrosive coatings with high solid content were successfully synthesized based on sunflower oil (SFO) via a polyesterification approach. These resins are characterized by energy-efficient polymer synthesis, lack of gelation properties, high functionality, and low viscosity. A chemical precipitation process was used to fabricate zinc oxide (ZnO) spherical nanostructures with controlled diameters and morphologies. A series of conformal, novel, low-cost SFO-based hyperbranched alkyd/spherical ZnO nanocomposites were fabricated through an ex situ method. Various nanofiller concentrations were distributed to establish synergetic effects on the micro-nano binary scale performance of the materials. The features of the nanocomposites, including the molecular weight, acid and hydroxyl values of the prepared alkyd resins, were concomitantly assessed through various standard tests. The nanocomposites were also subjected to various tests to determine their surface adhesion and mechanical properties, such as impact, T-bending, crosscut, and abrasion resistance tests. Furthermore, the physico-mechanical properties, anticorrosive behavior, thermal stabilities and cellular cytotoxicities of the fabricated materials were assessed. The anticorrosive features of the nanocomposites were investigated through salt spray tests in 5 wt% NaCl. The results indicate that well-dispersed ZnO nanospheres (0.5%) in the interior of the hyperbranched alkyd matrix improve the durability and anticorrosive attributes of the composites; thus, they exhibit potential applications in eco-friendly surface coatings

Mesoscopic open-eye core–shell spheroid carved anode/cathode electrodes for fully reversible and dynamic lithium-ion battery models

We report on the key influence of mesoscopic super-open-eye core–shell spheroids of TiO2- and LiFePO4-wrapped nanocarbon carved anode/cathode electrodes with uniform interior accommodation/storage pockets for the creation of fully reversible and dynamic Li-ion power battery (LIB) models. The mesoscopic core–shell anode/cathode electrodes provide potential half- and full-cell LIB-CR2032 configuration designs, and large-scale pouch models. In these variable mesoscopic LIB models, the broad-free-access and large-open-eye like gate-in-transport surfaces featured electrodes are key factors of built-in LIBs with excellent charge/discharge capacity, energy density performances, and outstanding cycling stability. Mesoscopic open-eye spheroid full-LIB-CR2032 configuration models retain 77.8% of the 1st cycle discharge specific capacity of 168.68 mA h g−1 after multiple cycling (i.e., 1st to 2000th cycles), efficient coulombic performance of approximately 99.6% at 0.1C, and high specific energy density battery of approximately 165.66 W h kg−1 at 0.1C. Furthermore, we have built a dynamic, super-open-mesoeye pouch LIB model using dense packing sets that are technically significant to meet the tradeoff requirements and long-term driving range of electric vehicles (EVs). The full-pouch package LIB models retain a powerful gate-in-transport system for heavy loaded electron/Li+ ion storage, diffusion, and truck movement through open-ended out/in and then up/downward eye circular/curvy folds, thereby leading to substantial durability, and remarkable electrochemical performances even after long-life charge/discharge cycling

EFFECT OF STRAIN, SEASON AND BREEDER'S AGE ON SOME PHYSIOLOGICAL PARAMETERS FOR LAYER DEAD EMBRYO

The effect of breederʼs strain (Hy-line Brown and Hy-line White W36) flock aged (28, 36, 46, 55, 66 weeks of age) and breeding season on some physiological measurements in egg, demand organs, dead supply organs and lymphoid organs were examined. At each age, 10 unhatched eggs from each genotype were examined on embryo weight percentage, Chorioallotoic  weight %, albumen pH %, yolk pH %, residual yolk %, pectoral  muscles weight %, hatch muscles weight %, heart, liver, lunges, spleen, burse weight percentage. There was a significant difference between layer breeder genotypes for lunges weight percentage the brown was higher (0.84) than the white (0.62). The breeding season had a significant effect on heart, liver, lunges weight percentages in winter higher than summer but yolk pH and residual yolk the summer higher than the winter. Breeder's age significant effect on embryo weight percentage, Chorioallotoic weight %, albumen pH %, yolk pH%, residual yolk %, hatch muscles weight % heart, liver, lunges and burse weight percentage.  It could be observed that the genetic differences among layer breeder strain and age of breeders can affect the hatchery performance. Additionally, handling of hatching eggs and incubation management can play a major role to overcome numerous hatching problems. In turn, increasing the production of table eggs and achieves the balance between supply and demand of that strategic commodity in Egyptian market

SYNTHESIS AND ANTIVIRAL ACTIVITY OF NOVEL ETHYL 2-(3-HETEROCYCLE-1H-INDOL-1-YL) ACETATE DERIVATIVES

Objective: Marek's disease (MD) is a widespread, herpesvirus-induced neoplastic disease in the domestic chicken that is caused by Marek's disease virus (MDV). Marek’s disease virus (MDV) belongs to the alphaherpesvirus family such as Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2). Recently Bag and co-workers 2014 reported that, 7-methoxy-1-methyl-4, 9-dihydro-3H-pyrido [3, 4-b]indole (Harmaline) showed potent anti-HSV-1 activity against both wild type and clinical isolates of HSV-1. The present work aimed to synthesize some new heterocyclic systems incorporated to indole moiety starting from ethyl 2-(3-acetyl-1H-indol-1-yl)acetate (1) in order to evaluate their antiviral activity in a trail to explore potential antiviral agents against MDV to limit the disease course and losses. Methods: Reaction of ethyl 2-(3-acetyl-1H-indol-1-yl) acetate (1) with semicarbazide hydrochloride yielded semicarbazone derivative 2. The oxidative cyclization of 2 using thionyl chloride and selenium dioxide afforded 1, 2, 3-thia and 1, 2, 3-selenadiazole derivatives 3 and 4, respectively. On the other hand, reaction of 1 with 4-chloro and 4-nitrobenzaldehydes under Claisen-Schmidt conditions gave α, β-unsaturated keto derivatives 5a, b. Cyclization of 5a, b using hydrazine hydrate, phenyl hydrazine, urea, thiourea or guanidine led to the formation of pyrazoles 6a, b, 7a, b, and pyrimidines derivatives 8a, b-10a, b; respectively. Condensation of 1 with phenyl hydrazine followed by Vilsmeier Haack formylation gave pyrazole-4-carboxaldehyde derivative 12. Reaction of aldehydic function group of 12 with different reagents led to the formation of pyrazol-5-ones 14-16, thiazolidinone 18, aziditine 19, 1, 6-diaminopyridine 21, triazolo(1, 5-a)pyridine 22 and pyrano(2, 3-c) pyrazole derivatives 23. The in vitro antiviral activity of the selected compounds 6a, b 7a, b 8a, b 9a, b and 10a, b was studied against Marek's disease virus (MDV). Results: Chicken embryo experiment showed that compounds 7b, 8b, 9b and 10a possessed significant antiviral activity with IC50 ranged between 5 and 6 µg/ml and substantial therapeutic indices (TI) of 80 and 83 were recorded. Cytotoxicity assay indicated that CC50 of 7b, 8b, 9b and 10 were greater than 400 and 500 mg/ml. Conclusion: Compounds 7b, 8b, 9b and 10a showed promising effect as anti-MDV infectivity application

Progress in biomimetic leverages for marine antifouling using nanocomposite coatings

Because of the environmental and economic casualties of biofouling on maritime navigation, modern studies have been devoted toward formulating advanced nanoscale composites in the controlled development of effective marine antifouling self-cleaning surfaces. Natural biomimetic surfaces have the advantages of micro-/nanoroughness and minimized free energy characteristics that can motivate the dynamic fabrication of superhydrophobic antifouling surfaces. This review provides an architectural panorama of the biomimetic antifouling designs and their key leverages to broaden horizons in the controlled fabrication of nanocomposite building blocks as force-driven marine antifouling models. As primary antifouling designs, understanding the key functions of surface geometry, heterogeneity, superhydrophobicity, and complexity of polymer/nanofiller composite building blocks on fouling-resistant systems is crucial. This review also discusses a wide range of fouling release coating systems that satisfy the growing demand in a sustainable future environment. For instance, the integration of block, segmented copolymer-based coatings and inorganic–organic hybrid nanofillers enhanced the model's antifouling properties with mechanical, superhydrophobic, chemically inert, and robust surfaces. These nanoscale antifouling systems offered surfaces with minimized free energy, micro-/nanoroughness, anisotropic heterogeneity, superior hydrophobicity, tunable non-wettability, antibacterial efficiency, and mechanical robustness. The confined fabrication of nanoscale orientation, configuration, arrangement, and direction along the architectural composite building blocks would yield excellent air-entrapping ability along the interfacial surface grooves and interfaces, which optimized the antifouling coating surfaces for long-term durability. This review provides systematic evidence of the effect of structurally folded nanocomposites, nanofiller tectonics, and building blocks on the creation of outstanding superhydrophobicity, self-cleaning surfaces, and potential antifouling coatings. The development of modern research gateways is a candidate for the sustainable future of antifouling coatings

Complex Structure Model Mutated Anode/Cathode Electrodes for Improving Large-Scale Battery Designs

We fabricate diverse geometric scales of lithium-ion battery (LIB) pattern assemblies in CR2032-circular coin designs by using complex building-block (CBB) anode/cathode electrodes as hierarchical models. The CBB anode/cathode electrode architectonics are designed with multiple complex hierarchies, including uni-, bi-, and tri-modal morphologies, multi-directional configurations, geometrical assemblies oriented in nano-/micro-scale structures, and surface mesh topologies, which allow us to leverage half- and full-cell CBB–LIB models. The CBB–LIB CR2032-circular coin designs have a Coulombic efficacy of ~99.7% even after 2000th lithiation/delithiation (discharge/charge) cycles, an outstanding battery energy density of 154.4 Wh/kg, and a specific discharge capacity of 163.6 mAh/g from 0.8 V to 3.5 V and at 0.1 C. The architectonic configurations and geometrics of the modulated full-cell CBB–LIB CR2032-circular designs play key roles in creating sustainable, full-scale CBB-mutated-LIBs with continuous and non-resisted surface transports and in achieving a sensible distribution of electron/Li+ ions. With hierarchical uni-, bi-, and tri-modal complexities, a dense collar packing f anode/cathode CBBmutated- LIB pouch-type sets in stacked layers can facilitate a rational design of CBB-pouch-type LIBs. Our CBB-mutated pouch-type LIB models have a sustainable Li+ ion-transport along multicomplex CBB-surfaces, substantial areal discharging capacity, and excellent volumetric- and gravimetric-cell energy densities and specific capacitances that fulfill the powerful force-driving range and tradeoff requirements in electric vehicle applications

Occupational exposure to dusts and risk of renal cell carcinoma

Background: Occupational exposures to dusts have generally been examined in relation to cancers of the respiratory system and have rarely been examined in relation to other cancers, such as renal cell carcinoma (RCC). Although previous epidemiological studies, though few, have shown certain dusts, such as asbestos, to increase renal cancer risk, the potential for other occupational dust exposures to cause kidney damage and/or cancer may exist. We investigated whether asbestos, as well as 20 other occupational dust exposures, were associated with RCC risk in a large European, multi-center, hospital-based renal case-control study.Methods: General occupational histories and job-specific questionnaires were reviewed by occupational hygienists for subject-specific information. Odds ratios (ORs) and 95% confidence intervals (95% CIs) between RCC risk and exposures were calculated using unconditional logistic regression. Results: Among participants ever exposed to dusts, significant associations were observed for glass fibres (OR: 2.1; 95% CI: 1.1-3.9), mineral wool fibres (OR: 2.5; 95% CI: 1.2-5.1), and brick dust (OR: 1.5; 95% CI: 1.0-2.4). Significant trends were also observed with exposure duration and cumulative exposure. No association between RCC risk and asbestos exposure was observed. Conclusion: Results suggest that increased RCC risk may be associated with occupational exposure to specific types of dusts. Additional studies are needed to replicate and extend findings. © 2011 Cancer Research UK All rights reserved