Usporedno istraživanje terapijskog učinka antiseptično-antibiotske paste za lokalno liječenje digitalnog dermatitisa u mliječnih krava.

The present study was designed to evaluate the effificacy of the concomitant use of antiseptic paste with a reduced amount of broad spectrum antibiotic as an alternative topical treatment of digital dermatitis (DD) in dairy cows. Thirty Holstein-Friesian dairy cows with active DD lesions were selected randomly from 93 diseased cases. Cows were randomly allocated for three topical treatment trials (10 each) using antiseptic paste (3 g iodine, 2 g potassium iodine and 30 g zinc oxide/100 g), antibiotic paste (250 mg/ 3gm of cefazolin) and antiseptic-antibiotic paste, under a light bandage for 72 hours. The clinical index scores and case defifinition scores were assessed on day 0, pre-treatment, and at 7, 14, 21 and 28 days post-treatment. The group treated with antiseptic-antibiotic paste showed significantly decreased scores of lesion depth (P<0.05) and size (P<0.05) when compared with the groups treated with antiseptic or antibiotic paste on the 21 and 28 days posttreatment. Collectively, the summation of clinical index scores showed a significant decrease (P<0.05) in the group treated with the antiseptic-antibiotic combination compared with the antiseptic and antibiotic groups at 14 days post-treatment. By the 28th day, the recovery rates were 9/10, 5/10 and 4/10 for the groups treated with the antiseptic-antibiotic combination, antibiotic or antiseptic paste, respectively. In conclusion, our results suggest the clinically synergistic effect of the topical antiseptic-antibiotic combination, which appears to be an effective alternative treatment for DD in dairy cows, minimizing the amount of antibiotic residues.Istraživanje je provedeno radi procjene učinkovitosti istodobne primjene antiseptične paste sa smanjenom količinom antibiotika širokog spektra kao alternative za lokalno liječenje digitalnog dermatitisa (DD) u mliječnih krava. Trideset mliječnih krava holštajnsko-frizijske pasmine s aktivnim DD nasumce je bilo izabrano iz skupine od 93 oboljelih. Krave su bile nasumce raspoređene u tri pokusne skupine po 10 životinja. Jedna skupina bila je liječena antiseptičnom pastom (3 g joda, 2 g kalijeva jodida i 30 g cinkova oksida/100 g), druga antibiotskom pastom (250 mg/3 gm cefazolina), a treća antiseptično-antibiotskom pastom. Sve su životinje bile s laganim zavojem tijekom 72 sata. Klinički bodovni indeks i rezultati za pojedini slučaj bili su procijenjeni 0-tog dana, tj. prije liječenja te 7., 14., 21. i 28. dana nakon liječenja. Skupina liječena antiseptično-antibiotskom pastom ocijenjena je sa značajno manjim brojem bodova 21. i 28. dana nakon liječenja s obzirom na dubinu (P<0,05) i veličinu (P<0,05) lezija u usporedbi sa skupinama koje su bile liječene antiseptičnom ili antibiotskom pastom. Zbroj kliničkog bodovnog indeksa bio je značajno smanjen (P<0,05) u skupini liječenoj antiseptičnoantibiotskom pastom u usporedbi s rezultatima za antiseptičnu ili antibiotsku skupinu 14. dana nakon liječenja. 28. dana broj oporavljenih u skupini liječenih antiseptično-antibiotskom pastom iznosio je 9/10, u skupini liječenih antibiotskom pastom bio je 5/10, a u skupini liječenih antiseptičnom pastom broj izliječenih bio je 4/10. Zaključno se može reći da rezultati upućuju na sinergistički učinak lokalno primijenjenog antiseptičnoantibiotskog pripravka što se može rabiti za liječenje DD u mliječnih krava i za smanjenje količine rezidua

EFFECT OF A BINARY BLEND COMPOSED OF POLOXAMER AND PVP ON AQUEOUS SOLUBILITY OF GRISEOFULVIN

Objective: The aim of this study was to improve the aqueous solubility of a poorly water soluble drug griseofulvin.Methods: To accomplish this goal a technique of solid dispersion with polyvinyl pyrolidone (k30) and poloxamer 407 was used. Solubility of griseofulvin alone and the solid dispersion were performed in 10 ml purified water and measured spectrophotometricaly atλ max 295 nm to detect the coefficient of improvement.Results: Solubility of griseofulvin alone was found to be 0.812 mg and the solubility of solid dispersion ranged from1.64 to 9.56 mg. After generating polynomial models correlating the variables using a D-Optimal mixture design, an optimal formulation with desired response was proposed by the statistical package. For validation, a new solid dispersion formulation based on the optimized composition was prepared and tested for solubility of griseofulvin. The optimized solid dispersion formulation enhances the solubility of griseofulvin by about 12 folds. Increase the amount of poloxamer in the optimized formulation increases the griseofulvin solubility to about 6 folds more than the optimized form.Conclusion: solid dispersion technique in conjunction with statistical design was shown to be very efficient for the optimization and improvement of aqueous solubility of poorly soluble drugs.Â

Flotation-separation of toxic metal ions from aqueous solutions using thiosemicarbazide derivatives as chelating agents and oleic acid as a surfactant

A simple and rapid procedure was developed for flotation-separation of toxic metal ions namely Hg2+, Mn2+ and Cd2+ from aqueous solutions. Thiosemicarbazide derivatives such as: 1-(amino-N-phenylmethanethio)-4-(pyridine-2-yl)thiosemicarbazide (H2PPS), N-phenyl-2-(pyridine-2-ylcarbamothioyl)hydrazinecarboxamide (H2PBO), 1-(amino(thioformyl)-N-phenylform)-4-(pyridine-2-yl)thiosemicarbazide (H2APO), and 1-(amino-N-(pyridine-3-yl) methanethio)-4-(pyridine-2-yl)thiosemicarbazide (H2PPY) have been used as organic chelating agents and oleic acid (HOL) as a surfactant. The different parameters affecting the flotation process namely, metal ion, ligands and surfactant concentrations, foreign ions (which are normally present in fresh and saline waters), pH and temperature are examined. About 100% of mercury, cadmium and manganese ions float at room temperature (~ 25 oC), at a metal:ligand ratio of 1:2  and at pH ~5. The procedure was successfully applied to recover Hg2+, Mn2+ and Cd2+ ions spiked into some water samples. The flotation mechanism is suggested based on some physical and chemical studies on the ligands and metal-complexes isolated from the floated layers

Bioactive compounds from Acokanthera oblongifolia

One cardiotonic glycoside, three triterpenes and one steroidal glycoside were isolated from Acokanthera oblongifolia fruits (pericarp) growing in Libya. Their structures were investigated by extensive application of IR, MS, 1DNMR and 2DNMR spectroscopy. The isolated compounds have evidenced in-vitro cytotoxicity on selected human cell lines (A-549, H-1299) when compared to doxorubicin. Keywords: Cardenolide; Acokanthera oblongifolia; antitumor activity; lung carcinoma cell line (A-549, H-1299)

Benign prostatic hyperplasia: Enucleation versus resection using plasmakinetic energy: a prospective randomized study at Zagazig University Hospital

Background: Transuretheral resection of the prostate (TURP) has been the gold standard endoscopic treatment for bladder outlet obstruction (BOO) secondary to benign prostatic hyperplasia (BPH). New technologies have been developed to minimize the morbidity of TURP. Recently, the Gyrus Plasma Kinetic (PK) System is the first bipolar device used in urological practice, as a new modality in treatment of BPH.Objective: To evaluate the efficacy and safety of plasmakinetic enucleation compared to plasmakinetic resection of the prostate in the management of BOO induced by BPH. Patients and Methods: This study was conducted on 58 volunteers from Department of Urology, Faculty of Medicine, Zagazig University between January 2018, and January 2020. Patients were randomized to either plasmakinetic enucleation of the prostate (PKEP) group or plasmakinetic resection of the prostate (PKRP) group. All patients were indicated for surgical treatment (prostate size ≥ 60 g and ≤ 120 g). Results: There were no statistically significant differences between the two groups preoperatively. PKEP resulted in a greater volume of prostatic tissue removal than the PKRP. Tissue retrieved/total operative time in PKEP group was greater than in PKRP group (0.69 gm/min vs 0.67 gm/min respectively). The mean indwelling uretheral catheter time was shorter in PKEP group (34.7 ±4.40 hrs.) than in PKRP (48.79 ±4.31 hrs.). Regarding postoperative complication (early and late), there were no statistically significant differences between the two groups.Conclusions: We concluded that regarding, surgical safety and efficacy PKEP is comparable to PKRP for prostates (60-120 ml). Either PKEP or PKRP can be on an equal footing to TURP as an endoscopic management of BP

Clinical Utility of Melatonin in Fibromyalgia Diagnosis

       Fibromyalgia syndrome (FMS) is a chronic disease with an unknown etiology, which is characterized by reduced pain threshold (hyperallgesia) &amp; pain with normally innocuous stimuli (allodynia).This diffuse pain is often disease associated  with wide range of  other symptoms including fatigue, sleep disturbance, stiffness&amp; more.FMS often occur concomitantly  with other rheumatologic disease such as rheumatoid arthritis(RA), systemic lupus erthymatosus(SLE).       The pineal hormone melatonin (MT) exerts a variety of effects on the immune system. MT activates immune cells and enhances inflammatory cytokine and nitric oxide production.Methods: We were studied 75 subjects, 55 of subjects were FMS patients defined by the American Colleague of Rheumatology (ACR 2010) criteria. Patients' mean age was 32.5 ± 13.9 years. They were classified into two groups: Group I of 25  primary FMS patients . Group II of 30 secondary FMS patients with other rheumatologic disease such as RA, SLE. Twenty age and sex matched healthy individuals were included in the study as a control group.Results:Mean Melatonin titers were significantly reduced (p&lt;0.0001) in primary FMs patients compared to the controls (21.32vs. 30.9 pg/ml), but they were significantly elevated (p&lt;0.0001) in secondary FMS compared to controls (138.1vs.30.9 pg/ml). Our data imposed that, in 1ry FMS there were negative correlations of MT titers with tender points (r=-0.848**,p&lt;0.0001), sleep disturbance(r=-0.963**, p&lt; 0.0001**), Fatigue (r= -0.972**, p&lt;0.001**), WPI (r= -0,953 **, p&lt;0.0001) and SS (r=-0.901**, p&lt; 0.0001). Conclusions:In primary FMS patients melatonin level is lower than melatonin level in control, but MT level is high in secondary FMS patients. There was a negative correlation between MT with tender points, sleep disturbance, fatigue, SS &amp; WPI. But there was a positive correlation between MT &amp; cognitive symptoms

Plasma microRNA-192 expression as a potential biomarker of diabetic kidney disease in patients with type 2 diabetes mellitus

Background. Albuminuria is an early clinical indicator of diabetic kidney disease (DKD). However, it has several limitations. The aim of this study was to evaluate the plasma microRNA-192 (miRNA-192) expression and its diagnostic performance in patients with type 2 diabetes mellitus (T2DM) and DKD.Methods. In this case-control study, 75 subjects were included into 3 groups: group (1): 20 patients with T2DM and UACR (urinary albumin creatinine ratio) &lt; 30 mg/gm, group (2): 30 patients with T2DM and ACR ≥ 30 mg/gm, and group (3): 25 healthy controls. Patients were recruited from the outpatient clinic of the Diabetes unit at our institution. Real-Time Quantitative Reverse Transcription PCR was used to assess plasma miRNA-192 expression.Results. Plasma miRNA-192 was significantly higher in T2DM patients with DKD compared to those with normal UAE. Additionally, in patients with T2DM, plasma miRNA-192 was positively correlated with UACR. The ROC curve analysis for miRNA-192 plasma expression in patients with T2DM, revealed that miRNA-192 had a good diagnostic performance (AUC = 0.778) to define T2DM patients with DKD.Conclusion. Plasma expression of miRNA-192 was able to discriminate increased UAE among patients with T2DM; suggesting a promising role for miRNA-192 as a potential biomarker for DKD

Smart android based home automation system using internet of things (IoT)

Recently, home automation system has getting significant attention because of the fast and advanced technology, making daily living more convenient. Almost everything has been digitalized and automated. The development of home automation will become easier and more popular because of the use of the Internet of Things (IoT). This paper described various interconnection systems of actuators, sensors to enable multiple home automation implementations. The system is known as HAS (Home automation system). It operates by connecting the robust Application Programming Interface (API), which is the key to a universal communication method. The HAS used devices, often implemented the actuators or sensors that have an upwards communication network followed by HAS (API). Most of the devices of the HAS (home automation system) used Raspberry Pi boards and ESP8285 chips. A smartphone application has been developed that allows users to control a wide range of home appliances and sensors from their smartphones. The application is user-friendly, adaptable, and beneficial for consumers and disabled people. It has the potential to be further extended via the use of various devices. The main objectives of this work are to make our home automation system, more secure and intelligent. HAS is a highly effective and efficient computational system that may be enhanced with a variety of devices and add-ons

Amelogenesis Imperfecta; Genes, Proteins And Pathways

Amelogenesis imperfecta (AI) is the name given to a heterogeneous group of conditions characterised by inherited developmental enamel defects. AI enamel is abnormally thin, soft, fragile, pitted and/or badly discoloured, with poor function and aesthetics, causing patients problems such as early tooth loss, severe embarrassment, eating difficulties and pain. It was first described separately from diseases of dentine nearly 80 years ago, but the underlying genetic and mechanistic basis of the condition is only now coming to light. Mutations in the gene AMELX, encoding an extracellular matrix protein secreted by ameloblasts during enamel formation, were first identified as a cause of AI in 1991. Since then, mutations in at least eighteen genes have been shown to cause AI presenting in isolation of other health problems, with many more implicated in syndromic AI. Some of the encoded proteins have well documented roles in amelogenesis, acting as enamel matrix proteins or the proteases that degrade them, cell adhesion molecules or regulators of calcium homeostasis. However, for others, function is less clear and further research is needed to understand the pathways and processes essential for the development of healthy enamel. Here, we review the genes and mutations underlying AI presenting in isolation of other health problems, the proteins they encode and knowledge of their roles in amelogenesis, combining evidence from human phenotypes, inheritance patterns, mouse models and in vitro studies. An LOVD resource (http://dna2.leeds.ac.uk/LOVD/) containing all published gene mutations for AI presenting in isolation of other health problems is described. We use this resource to identify trends in the genes and mutations reported to cause AI in the 270 families for which molecular diagnoses have been reported by 23rd May 2017. Finally we discuss the potential value of the translation of AI genetics to clinical care with improved patient pathways and speculate on the possibility of novel treatments and prevention strategies for AI