521 research outputs found
Using infrared spectral features to probe circumstellar dust shells around cool stars
IRAS observations of cool stars provide low resolution spectra in the mid-infrared and also give fluxes at four wavelength bands from which color-color diagrams are constructed. The later have been used to study the evolution of these stars: as an O-rich star evolves to become a C-rich star and its detached dust shell moves further away, its evolution can be tracked on a color-color diagram. A major factor in determining the position of either C-rich or O-rich stars on the 12-25-60 micron color-color diagram is the presence of spectral features in the mid-IR. O-rich stars show a 9.8 micron silicate feature, while C-rich stars have a SiC feature at 11.2 microns. IRAS observations indicate that the SiC feature is quite narrow and uniform in shape showing little variation from star to star. The full width at half maximum (FWHM) is 1.6 + or - 0.15 microns. On the other hand, the shape of the silicate feature varies widely among the O-rich stars, with a FWHM ranging from 2 to 3 microns. The characteristics of circumstellar dust shells should manifest themselves both in the flux spectrum and in the details of the spectral features. To provide a coherent interpretation for these IRAS observations, models were constructed (using a radiative transfer code) of dust shells around O-rich and C-rich stars. Realistic grain opacities were used which include spectral features of varying intrinsic widths (e.g., Gaussian features at 10 microns with half width at half maximum of 0.5 and 1.0 microns)
A Post-AGB Star in the Small Magellanic Cloud Observed with the Spitzer Infrared Spectrograph
We have observed an evolved star with a rare combination of spectral
features, MSX SMC 029, in the Small Magellanic Cloud (SMC) using the
low-resolution modules of the Infrared Spectrograph on the Spitzer Space
Telescope. A cool dust continuum dominates the spectrum of MSX SMC 029. The
spectrum also shows both emission from polycyclic aromatic hydrocarbons (PAHs)
and absorption at 13.7 micron from C2H2, a juxtaposition seen in only two other
sources, AFGL 2688 and IRAS 13416-6243, both post-asymptotic giant branch (AGB)
objects. As in these sources, the PAH spectrum has the unusual trait that the
peak emission in the 7-9 micron complex lies beyond 8.0 micron. In addition,
the 8.6 micron feature has an intensity as strong as the C-C modes which
normally peak between 7.7 and 7.9 micron. The relative flux of the feature at
11.3 micron to that at 8 micron suggests that the PAHs in MSX SMC 029 either
have a low ionization fraction or are largely unprocessed. The 13-16 micron
wavelength region shows strong absorption features similar to those observed in
the post-AGB objects AFGL 618 and SMP LMC 11. This broad absorption may arise
from the same molecules which have been identified in those sources: C2H2,
C4H2, HC3N, and C6H6. The similarities between MSX SMC 029, AFGL 2688, and AFGL
618 lead us to conclude that MSX SMC 029 has evolved off the AGB in only the
past few hundred years, making it the third post-AGB object identified in the
SMC.Comment: 4 figures, Fig. 4 color; to appear in the 20 November 2006
Astrophysical Journal Letter
Effect of Airborne-particle Abrasion on 3-dimensional Surface Roughness and Characteristic Failure Load of Fiber-reinforced Posts
Statement of problem Debonding is the most common complication of fiber-reinforced posts (FRPs). Airborne-particle abrasion (APA) has been suggested to increase resin cement adhesion to the surface of FRPs. However, which abrasion protocol is the most favorable is unclear. Purpose The purpose of this in vitro study was to compare the surface roughness and characteristic failure load of three FRP systems following different APA protocols. Material and methods A total of 150 posts from 3 manufacturers (glass FRP, quartz FRP, and zirconia-enriched glass FRP) were randomly assigned to different surface treatments (NT: no treatment—control; E0: cleaned with 96% ethanol solution; E2: APA for 2 seconds/mm2—ethanol cleaned, E5: APA for 5 seconds/mm2—ethanol cleaned; and E10: APA for 10 seconds/mm2—ethanol cleaned) forming 15 groups in total. APA was performed with 50-μm aluminum oxide. Each post was observed under a 3-dimensional (3D) laser microscope, and average 3D surface roughness (Sa) was measured. Failure was induced with a universal testing machine. Two specimens per group were evaluated under the same microscope to evaluate failure patterns. Surface roughness data were analyzed with the Welch ANOVA (α=.05), followed by the post hoc Games-Howell test. Failure load differences were determined by 2-parameter Weibull statistics and likelihood ratio contour plots (95% confidence bounds). Results
Statistically significant differences were found in the mean surface roughness among the groups (Welch ANOVA, P Conclusions APA significantly increased surface roughness in all post systems. APA effects on characteristic failure load were dependent on the material used
MSX, 2MASS, and the LMC: A Combined Near and Mid Infrared View
The Large Magellanic Cloud (LMC) has been observed by both the Midcourse
Space Experiment (MSX) in the mid-infrared and the Two Micron All Sky Survey
(2MASS) in the near-infrared. We have performed a cross-correlation of the 1806
MSX catalog sources and nearly 1.4 million 2MASS catalogued point and extended
sources and find 1664 matches. Using the available color information, we
identify a number of stellar populations and nebulae, including main sequence
stars, giant stars, red supergiants, carbon- and oxygen-rich asymptotic giant
branch (AGB) stars, planetary nebulae, H II regions, and other dusty objects
likely associated with early-type stars. 731 of these sources have no previous
identification. We compile a listing of all objects, which includes photometry
and astrometry. The 8.3 micron MSX sensitivity is the limiting factor for
object detection: only the brighter red objects, specifically the red
supergiants, AGB stars, planetary nebulae and HII regions, are detected in the
LMC. The remaining objects are likely in the Galactic foreground. The spatial
distribution of the infrared LMC sources may contribute to understanding
stellar formation and evolution and the overall galactic evolution. We
demonstrate that a combined mid- and near-infrared photometric baseline
provides a powerful means of identifying new objects in the LMC for future
ground-based and space-based follow-up observations.Comment: 23 pages, 10 figures, to appear in the AJ (2001 Oct issue). N.B:
Tables 2 & 3 corrected and available as html file
Circumstellar Structure around Evolved Stars in the Cygnus-X Star Formation Region
We present observations of newly discovered 24 micron circumstellar
structures detected with the Multiband Imaging Photometer for Spitzer (MIPS)
around three evolved stars in the Cygnus-X star forming region. One of the
objects, BD+43 3710, has a bipolar nebula, possibly due to an outflow or a
torus of material. A second, HBHA 4202-22, a Wolf-Rayet candidate, shows a
circular shell of 24 micron emission suggestive of either a limb-brightened
shell or disk seen face-on. No diffuse emission was detected around either of
these two objects in the Spitzer 3.6-8 micron Infrared Array Camera (IRAC)
bands. The third object is the luminous blue variable candidate G79.29+0.46. We
resolved the previously known inner ring in all four IRAC bands. The 24 micron
emission from the inner ring extends ~1.2 arcmin beyond the shorter wavelength
emission, well beyond what can be attributed to the difference in resolutions
between MIPS and IRAC. Additionally, we have discovered an outer ring of 24
micron emission, possibly due to an earlier episode of mass loss. For the two
shell stars, we present the results of radiative transfer models, constraining
the stellar and dust shell parameters. The shells are composed of amorphous
carbon grains, plus polycyclic aromatic hydrocarbons in the case of
G79.29+0.46. Both G79.29+0.46 and HBHA 4202-22 lie behind the main Cygnus-X
cloud. Although G79.29+0.46 may simply be on the far side of the cloud, HBHA
4202-22 is unrelated to the Cygnus-X star formation region.Comment: Accepted by A
A Spitzer IRS Spectral Atlas of Luminous 8 micron Sources in the Large Magellanic Cloud
We present an atlas of Spitzer Space Telescope Infrared Spectrograph (IRS)
spectra of highly luminous, compact mid-infrared sources in the Large
Magellanic Cloud. Sources were selected on the basis of infrared colors and 8
micron (MSX) fluxes indicative of highly evolved, intermediate- to high-mass
stars with current or recent mass loss at large rates. We determine the
chemistry of the circumstellar envelope from the mid-IR continuum and spectral
features and classify the spectral types of the stars. In the sample of 60
sources, we find 21 Red Supergiants (RSGs), 16 C-rich Asymptotic Giant Branch
(AGB) stars, 11 HII regions, 4 likely O-rich AGB stars, 4 Galactic O-rich AGB
stars, 2 OH/IR stars, and 2 B[e] supergiants with peculiar IR spectra. We find
that the overwhelming majority of the sample AGB stars (with typical IR
luminosities ~1.0E4 L_sun) have C-rich envelopes, while the O-rich objects are
predominantly luminous RSGs with L_IR ~ 1.0E5 L_sun. We determine mean
bolometric corrections to the stellar K-band flux densities and find that for
carbon stars, the bolometric corrections depend on the infrared color, whereas
for RSGs, the bolometric correction is independent of IR color. Our results
reveal that objects previously classified as PNe on the basis of IR colors are
in fact compact HII regions with very red IRS spectra that include strong
atomic recombination lines and PAH emission features. We demonstrate that the
IRS spectral classes in our sample separate clearly in infrared color-color
diagrams that use combinations of 2MASS data and synthetic IRAC/MIPS fluxes
derived from the IRS spectra. On this basis, we suggest diagnostics to identify
and classify, with high confidence levels, IR-luminous evolved stars and HII
regions in nearby galaxies using Spitzer and near-infrared photometry.Comment: 46 pages, 9 figures; accepted for publication in AJ; abstract
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CRISPR/Cas9 knockouts reveal genetic interaction between strain-transcendent erythrocyte determinants of Plasmodium falciparum invasion.
During malaria blood-stage infections, Plasmodium parasites interact with the RBC surface to enable invasion followed by intracellular proliferation. Critical factors involved in invasion have been identified using biochemical and genetic approaches including specific knockdowns of genes of interest from primary CD34+ hematopoietic stem cells (cRBCs). Here we report the development of a robust in vitro culture system to produce RBCs that allow the generation of gene knockouts via CRISPR/Cas9 using the immortal JK-1 erythroleukemia line. JK-1 cells spontaneously differentiate, generating cells at different stages of erythropoiesis, including terminally differentiated nucleated RBCs that we term "jkRBCs." A screen of small-molecule epigenetic regulators identified several bromodomain-specific inhibitors that promote differentiation and enable production of synchronous populations of jkRBCs. Global surface proteomic profiling revealed that jkRBCs express all known Pfalciparum host receptors in a similar fashion to cRBCs and that multiple Pfalciparum strains invade jkRBCs at comparable levels to cRBCs and RBCs. Using CRISPR/Cas9, we deleted two host factors, basigin (BSG) and CD44, for which no natural nulls exist. BSG interacts with the parasite ligand Rh5, a prominent vaccine candidate. A BSG knockout was completely refractory to parasite invasion in a strain-transcendent manner, confirming the essential role for BSG during invasion. CD44 was recently identified in an RNAi screen of blood group genes as a host factor for invasion, and we show that CD44 knockout results in strain-transcendent reduction in invasion. Furthermore, we demonstrate a functional interaction between these two determinants in mediating Pfalciparum erythrocyte invasion
R CrB Candidates in the Small Magellanic Cloud: Observations of Cold, Featureless Dust with the Spitzer Infrared Spectrograph
We observed 36 evolved stars in the Small Magellanic Cloud (SMC) using the
low-resolution mode of the Infrared Spectrograph (IRS) on the Spitzer Space
Telescope. Two of these stars, MSX SMC 014 and 155, have nearly featureless
spectral energy distributions over the IRS wavelength range (5.2-35 um) and
F_nu peaking at ~8-9 um. The data can be fit by sets of amorphous carbon shells
or by single 600-700 K blackbodies. The most similar spectra found in extant
spectral databases are of R CrB, although the spectral structure seen in R CrB
and similar stars is much weaker or absent in the SMC sources. Both SMC stars
show variability in the near-infrared. Ground-based visual spectra confirm that
MSX SMC 155 is carbon-rich, as expected for R CrB (RCB) stars, and coincides
with an object previously identified as an RCB candidate. The temperature of
the underlying star is lower for MSX SMC 155 than for typical RCB stars. The
strength of the C_2 Swan bands and the low temperature suggest that it may be a
rare DY Per-type star, only the fifth such identified. MSX SMC 014 represents a
new RCB candidate in the SMC, bringing the number of RCB candidates in the SMC
to six. It is the first RCB candidate discovered with Spitzer and the first
identified by its infrared spectral characteristics rather than its visual
variability.Comment: Accepted by ApJ Letters 25 August, 2005, 4 pages (emulateapj), 5
figure
UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction.
Brown adipose tissue (BAT) mitochondria exhibit high oxidative capacity and abundant expression of both electron transport chain components and uncoupling protein 1 (UCP1). UCP1 dissipates the mitochondrial proton motive force (Δp) generated by the respiratory chain and increases thermogenesis. Here we find that in mice genetically lacking UCP1, cold-induced activation of metabolism triggers innate immune signaling and markers of cell death in BAT. Moreover, global proteomic analysis reveals that this cascade induced by UCP1 deletion is associated with a dramatic reduction in electron transport chain abundance. UCP1-deficient BAT mitochondria exhibit reduced mitochondrial calcium buffering capacity and are highly sensitive to mitochondrial permeability transition induced by reactive oxygen species (ROS) and calcium overload. This dysfunction depends on ROS production by reverse electron transport through mitochondrial complex I, and can be rescued by inhibition of electron transfer through complex I or pharmacologic depletion of ROS levels. Our findings indicate that the interscapular BAT of Ucp1 knockout mice exhibits mitochondrial disruptions that extend well beyond the deletion of UCP1 itself. This finding should be carefully considered when using this mouse model to examine the role of UCP1 in physiology
Hubble Space Telescope NICMOS Polarization Observations of Three Edge-on Massive YSOs
Massive young stellar objects (YSOs), like low-mass YSOs, appear to be
surrounded by optically thick envelopes and/or disks and have regions, often
bipolar, that are seen in polarized scattered light at near-infrared
wavelengths. We are using the 0.2'' spatial resolution of NICMOS on Hubble
Space Telescope to examine the structure of the disks and outflow regions of
massive YSOs in star-forming regions within a few kpc of the Sun. Here we
report on 2 micron polarimetry of NGC 6334 V and S255 IRS1. NGC 6334 V consists
of a double-lobed bright reflection nebula seen against a dark region, probably
an optically thick molecular cloud. Our polarization measurements show that the
illuminating star lies ~ 2'' south of the line connecting the two lobes; we do
not detect this star at 2 micron, but there are a small radio source and a
mid-infrared source at this location. S255 IRS1 consists of two YSOs (NIRS1 and
NIRS3) with overlapping scattered light lobes and luminosities corresponding to
early B stars. Included in IRS1 is a cluster of stars from whose polarization
we determine the local magnetic field direction. Neither YSO has its scattered
light lobes aligned with this magnetic field. The line connecting the scattered
light lobes of NIRS1 is twisted symmetrically around the star; the best
explanation is that the star is part of a close binary and the outflow axis of
NIRS1 is precessing as a result of non-coplanar disk and orbit. The star NIRS3
is also offset from the line connecting its two scattered light lobes. We
suggest that all three YSOs show evidence of episodic ejection of material as
they accrete from dense, optically thick envelopes.Comment: 39 pages, 7 figures, 4 tables To be published in The Astrophysical
Journa
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